Razi Vaccine & Serum Research Institute

Karaj, Iran

Razi Vaccine & Serum Research Institute

Karaj, Iran
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Asgari S.,Sharif University of Technology | Bagheri H.,Sharif University of Technology | Es-haghi A.,Razi Vaccine & Serum Research Institute | AminiTabrizi R.,Sharif University of Technology
Journal of Chromatography A | Year: 2017

An imprinted interpenetrating polymer network (IPN) was synthesized and used as a medium for isolation of carbamazepine from urine samples. The polymer network consisted of a homogeneous polystyrene–sol gel hybrid constructed by in–situ radical polymerization method. In this process, within the sol–gel reaction duration, styrene monomer could penetrate into the reaction mixture and after the polymerization initiation, a monolithic IPN structure was prepared. The scanning electron microscopy (SEM) image and energy dispersive spectroscopy (EDX) are indications of the polystyrene dispersion at nano- to micro-meter level in the sol gel matrix. Eventually, the synthesized IPN was used as a sorbent in microextraction in packed syringe (MEPS) combined with high performance liquid chromatography (HPLC) for isolation of carbamazepine, naproxen and dexamethasone from urine samples. The molecularly imprinted IPN showed some degree of selectivity towards carbamazepine. To assess the important parameters influencing the extraction and desorption processes, an experimental design strategy was used. By the current method, low limits of detection (1.3–1.5 μg L−1) and quantification (4.2–5 μg L−1) were achieved (hydrocortisone as the internal standard). The intra- and inter-day precision data at 50 and 300 μg L−1 were 1.3–7.4%, while the working linear dynamic range was from 4.2 to 500 μg L−1. © 2017 Elsevier B.V.

PubMed | Razi Vaccine & Serum Research Institute, Tehran University of Medical Sciences, University of Tabriz and Regional TB reference laboratory
Type: Journal Article | Journal: Revista da Sociedade Brasileira de Medicina Tropical | Year: 2016

INTRODUCTION Characterization of Mycobacterium tuberculosis (MTB) isolates by DNA fingerprinting has contributed to tuberculosis (TB) control. The aim of this study was to determine the genetic diversity of MTB isolates from Tehran province in Iran. METHODS MTB isolates from 60 Iranian and 10 Afghan TB patients were fingerprinted by standard IS6110-restriction fragment length polymorphism (RFLP) analysis and spoligotyping. RESULTS The copy number of IS6110 ranged from 10-24 per isolate. The isolates were classified into 22 clusters showing 80% similarity by RFLP analysis. Fourteen multidrug-resistant (MDR) isolates were grouped into 4 IS6110-RFLP clusters, with 10 isolates [71% (95% CI: 45-89%)] in 1 cluster, suggesting a possible epidemiological linkage. Eighteen Iranian isolates showed 80% similarity with Afghan isolates. There were no strains with identical fingerprints. Spoligotyping of 70 isolates produced 23 distinct patterns. Sixty (85.7%) isolates were grouped into 13 clusters, while the remaining 10 isolates (14.2%) were not clustered. Ural (formerly Haarlem4) (n = 22, 31.4%) was the most common family followed by Central Asian strain (CAS) (n = 18, 25.7%) and T (n = 9, 12.8%) families. Only 1strain was characterized as having the Beijing genotype. Among 60 Iranian and 10 Afghan MTB isolates, 25% (95% CI: 16-37) and 70% (95% CI: 39-89) were categorized as Ural lineage, respectively. CONCLUSIONS A higher prevalence of Ural family MTB isolates among Afghan patients than among Iranian patients suggests the possible transmission of this lineage following the immigration of Afghans to Iran.

PubMed | Lorestan University of Medical Sciences and Razi Vaccine Serum Research Institute
Type: Journal Article | Journal: International journal of high risk behaviors & addiction | Year: 2016

Hepatitis C is an infectious disease caused by blood-borne pathogen, hepatitis C virus (HCV).The purpose of this study was to investigate the prevalence of HCV infection and associated risk factors among addicts in drug treatment centers in Lorestan Province, Iran.A cross-sectional sero-behavioral survey was given to drug addicts in the drug treatment centers of Khorramabad, Lorestan Province, Iran during June 2012 - March 2013. Drug addicts were interviewed using a standard questionnaire including demographic, imprisonment history, and HCV-related risk behavior items. Thereafter, the sera drawn from the participants were tested for anti-HCV antibody (Ab), anti-human immunodeficiency virus (HIV) Ab, and hepatitis B surface antigen (HBsAg).The mean age of the cohorts was 31.7. Up to 60.2% of drug users had educational levels less than high school, 67.5% were self-employed, and 32.5% were office workers. The mean duration of drug injection was 6.8 years. Statistical analyses indicated that the prevalence of HCV among drug addicts was positively associated with age, past incarceration, drug injection history, the duration of drug use, and tattooing. In addition, 16.23% of volunteers were HCV-positive. Of those infected with HCV, 1.10% was co-infected with HBV, 2.95% were positive for HIV, and 0.36% of HCV-positive cases were infected with all three viruses.The high prevalence of HCV infection among this group implies a high rate of transmission and exposure to the risk of serious diseases. It is important that the high prevalence of HCV infection be taken into consideration to control further transmission of this infection.

PubMed | Razi Vaccine & Serum Research Institute and University of Tehran
Type: Journal Article | Journal: Advanced pharmaceutical bulletin | Year: 2016

The direct transmission of avian influenza viruses to human and increasing drug resisted strains posing new threats for public health. Therefore, development of efficient vaccines is needed to generate protective and persistent immunity to the viruses.Three motifs of Mx protein sequence in human, mouse and poultry located in interferon induced (GTP ase) domain were candidate as biologic adjuvant for enhancing the immune responses against influenza virus. Chimera proteins composed with the conserved HA2 subunit of influenza virus and the Mx motifs named HA2/Mx were modeled and evaluated by in silico analysis includes bioinformatics algorithms in order to explore biological characteristics of these peptides.Amongst the predicted models, HA2/Mx1 peptide showed the better results following protein structures prediction, antigenic epitopes determination and model quality evaluation. Comparative homology modeling was performed with Swiss Model and the model was validated using ProSA. Epitope predictions revealed the construct could induce both B and T cell epitopes that expect a high immune response.Taken together, these data indicate that the HA2/Mx1 chimera peptide can be potentiated for developing an adjuvant-fused influenza vaccine capable of stimulating effective immune response.

Shahsavandi S.,Razi Vaccine & Serum Research Institute | Ebrahimi M.M.,Razi Vaccine & Serum Research Institute | Sadeghi K.,Razi Vaccine & Serum Research Institute | Mahravani H.,Razi Vaccine & Serum Research Institute
Virologica Sinica | Year: 2015

Influenza viruses continue to emerge and re-emerge, posing new threats for public health. Control and treatment of influenza depends mainly on vaccination and chemoprophylaxis with approved antiviral drugs. Identification of specific epitopes derived from influenza viruses has significantly advanced the development of epitope-based vaccines. Here, we explore the idea of using HLA binding data to design an epitope-based vaccine that can elicit heterosubtypic T-cell responses against circulating H7N9, H5N1, and H9N2 subtypes. The hemokinin-1 (HK-1) peptide sequence was used to induce immune responses against the influenza viruses. Five conserved high score cytotoxic T lymphocyte (CTL) epitopes restricted to HLA-A*0201-binding peptides within the hemagglutinin (HA) protein of the viruses were chosen, and two HA CTL/HK-1 chimera protein models designed. Using in silico analysis, which involves interferon epitope scanning, protein structure prediction, antigenic epitope determination, and model quality evaluation, chimeric proteins were designed. The applicability of one of these proteins as a heterosubtypic epitopebased vaccine candidate was analyzed. © 2015, Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg.

PubMed | Razi Vaccine & Serum Research Institute and Razi University
Type: | Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences | Year: 2016

Electrospinning technique was used to convert polydimethyl siloxane (PDMS) sol-gel solution to a new nanostructure on a stainless steel wire. The surface morphology of the fiber was observed by scanning electron microscopy (SEM). It showed a diameter range of 30-60nm for PDMS nanoparticles with a homogeneous and porous surface structure. The applicability of this coating was assessed for the headspace SPME (HS-SPME) of benzene, toluene, ethylbenzene and xylenes (BTEX) from water samples followed by gas chromatography-mass spectrometry. The important parameters affecting extraction efficiency such as extraction time and temperature, desorption conditions, agitation rate and ionic strength were investigated and optimized. Under the optimized conditions, LODs and LOQs of 0.3-5gL(-1) and 1-10gL(-1) were obtained, respectively. The method showed linearity in the broad range of 1-5000gL(-1) with correlation coefficient of >0.99. Inter-day and intra-day precisions of the developed method ranged from 2.43% to 6.54% and from 5.24% to 13.73%, respectively. The thermal stability of the fiber was investigated on stainless steel wire. It was found to be durable at 260C for more than 360min. Furthermore, the proposed method was successfully applied for quantification of BTEX in real water samples.

PubMed | Razi Vaccine & Serum Research Institute, Islamic Azad University at Sāveh and Mashhad University of Medical Sciences
Type: | Journal: International journal of mycobacteriology | Year: 2017

Tuberculosis has long been recognized as a zoonotic disease and remains one of the most life-threatening diseases worldwide. In spite of the successful treatment of this disease, the World Health Organization considers it as a disease with the highest priority in 1993, and it is now the second leading cause of infectious mortality. Since the identification of Mycobacterium tuberculosis complex species is of special importance, the aim of this study was to identify the species from the submitted mycobacterial isolates of tuberculars in Khorasan Razavi sent to the cell reference laboratory of Razavi Institute by the molecular method.Today, various molecular methods are used as a useful and quick tool to identify the isolates. In this research, we used polymerase chain reaction (PCR) test on the 16sr RNA gene to determine if the isolates belonged to the Mycobacterium genus. Then another PCR test of IS6110 performed to confirm that all of isolates are belonging to M. tuberculosis complex. Finally RD typing method was used to differentiate the complex members, and the member species of the complex was identified by this method.Using molecular identification of 100 submitted isolates in Khorasan Razavi province, it was found that all isolates are M. tuberculosis. The results showed that the sanatorium in this province was not infected with M. bovis and other members of the complex. This is either due to the widespread use of pasteurized milk and nonuse of milk contaminated with M. bovis or due to the lack of regular tuberculosis control and eradication programs in the city.

PubMed | Razi Vaccine & Serum Research Institute
Type: | Journal: Advances in virology | Year: 2016

A cDNA clone for factor 10 (FX) isolated from chicken embryo inserted into the mammalian cell expression vector pCDNA3.1 was transfected into the baby hamster kidney (BHK-21) cell line. The generated BHK-21 cells with inducible expression of FX were used to investigate the efficacy of the serine transmembrane protease to proteolytic activation of influenza virus hemagglutinin (HA) with monobasic cleavage site. Data showed that the BHK-21/FX stably expressed FX after ten serial passages. The cells could proteolytically cleave the HA of low pathogenic avian influenza virus at multiplicity of infection 0.01. Growth kinetics of the virus on BHK-21/FX, BHK-21, and MDCK cells were evaluated by titrations of virus particles in each culture supernatant. Efficient multicycle viral replication was markedly detected in the cell at subsequent passages. Virus titration demonstrated that BHK-21/FX cell supported high-titer growth of the virus in which the viral titer is comparable to the virus grown in BHK-21 or MDCK cells with TPCK-trypsin. The results indicate potential application for the BHK-21/FX in influenza virus replication procedure and related studies.

PubMed | Razi Vaccine & Serum Research Institute and Mashhad University of Medical Sciences
Type: Journal Article | Journal: Reports of biochemistry & molecular biology | Year: 2016

Allergy is a clinical disorder affecting humans worldwide. Allergenic extracts prepared from natural source materials remain heterogeneous in composition and content, but are regularly used for diagnosis and immunotherapy. Recombinant allergens are suitable candidates to use in place of natural allergens; however, the recombinant allergens should be assessed and compared with the natural ones. Cuc m 2 (profilin), one of the most important allergens of melon (Cucumis melo), has been cloned and was expressed in Escherichia coli (E. coli). We aimed to evaluate the validity of recombinant Cuc m 2 (rCuc m 2) in the diagnosis of melon allergy and investigate whether rCuc m 2 could be used as a replacement for natural Cuc m 2 (nCuc m 2).nCuc m 2 was purified by immuno-affinity chromatography and rCuc m 2 was purified by metal-affinity chromatography. SDS-PAGE and western blotting were carried out to evaluate the purification methods. Skin prick tests (SPT), and enzyme immunoassays to determine specific IgE, were performed with the natural and recombinant purified allergens on 53 patients with melon allergy.rCuc m 2 elicited no significantly different responses in skin compared with nCuc m 2. All patients sera showed similar ODs in ELISAs with natural and recombinant profilin.rCuc m 2 evoked strong immuno-reactivity equivalent to nCuc m 2, and has potential for diagnosis of melon allergy.

PubMed | Razi Vaccine & Serum Research Institute and Mashhad University of Medical Sciences
Type: Journal Article | Journal: Reports of biochemistry & molecular biology | Year: 2016

Allergy is a clinical disorder affecting the human population with wide geographical distribution. Platanus orientalis (P. orientalis) trees are planted in many countries and their pollen causes allergic reactions. Cyclophilin has recently been identified as one of the most important allergens of P. orientalis pollen. We aimed to clone and purify this allergen in Escherichia coli for further studies and therapeutic and diagnostic purposes for allergy to P. orientalis .RNA was extracted from P. orientalis. A full-length fragment encoding cyclophilin was prepared by polymerase chain reaction amplification of the first-strand cDNA synthesized from P. orientalis RNA. The cDNA was inserted into the pET32b (+) vector, and the construct transformed into E. coli Top10 and BL21 cells. The expressed protein was purified by the CuSO4 method.The cDNA for the cyclophilin of P. orientalis pollen was cloned, and a specific reactivity of recombinant cyclophin was confirmed by immunoblotting using sera from patients allergic to P. orientalis pollen.The recombinant cyclophilin has a potential for immunologic assays for evaluation of allergy to P. orientalis pollen.

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