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Feyisa S.G.,Tehran University of Medical Sciences | Haeili M.,University of Tabriz | Zahednamazi F.,Tehran University of Medical Sciences | Mosavari N.,Razi Vaccine & Serum Research Institute | And 4 more authors.
Revista da Sociedade Brasileira de Medicina Tropical | Year: 2016

INTRODUCTION Characterization of Mycobacterium tuberculosis (MTB) isolates by DNA fingerprinting has contributed to tuberculosis (TB) control. The aim of this study was to determine the genetic diversity of MTB isolates from Tehran province in Iran. METHODS MTB isolates from 60 Iranian and 10 Afghan TB patients were fingerprinted by standard IS6110-restriction fragment length polymorphism (RFLP) analysis and spoligotyping. RESULTS The copy number of IS6110 ranged from 10-24 per isolate. The isolates were classified into 22 clusters showing ≥ 80% similarity by RFLP analysis. Fourteen multidrug-resistant (MDR) isolates were grouped into 4 IS6110-RFLP clusters, with 10 isolates [71% (95% CI: 45-89%)] in 1 cluster, suggesting a possible epidemiological linkage. Eighteen Iranian isolates showed ≥ 80% similarity with Afghan isolates. There were no strains with identical fingerprints. Spoligotyping of 70 isolates produced 23 distinct patterns. Sixty (85.7%) isolates were grouped into 13 clusters, while the remaining 10 isolates (14.2%) were not clustered. Ural (formerly Haarlem4) (n = 22, 31.4%) was the most common family followed by Central Asian strain (CAS) (n = 18, 25.7%) and T (n = 9, 12.8%) families. Only 1strain was characterized as having the Beijing genotype. Among 60 Iranian and 10 Afghan MTB isolates, 25% (95% CI: 16-37) and 70% (95% CI: 39-89) were categorized as Ural lineage, respectively. CONCLUSIONS A higher prevalence of Ural family MTB isolates among Afghan patients than among Iranian patients suggests the possible transmission of this lineage following the immigration of Afghans to Iran. © 2016, Sociedade Brasileira de Medicina Tropical. All rights reserved. Source

Gharib Mombeni E.,Animal Diseases Control and Monitoring | Mosavari N.,Razi Vaccine & Serum Research Institute | Keshavarz R.,Razi Vaccine & Serum Research Institute | Tadayon K.,Razi Vaccine & Serum Research Institute | And 2 more authors.
Iranian Journal of Public Health | Year: 2016

At present, most of Iran is free of bovine tuberculosis (TB). The strategy of control and eradication in Iran involves a tuberculation test and slaughter of reactors, a procedure transformed the present-day prevalence of TB into a sporadic occurrence. This paper describes the first report of bovine tuberculosis in a European fallow deer (Dama dama dama) in Iran. The deer was emaciated and found dead in the Hoveize Provincial Zoo Park. Post-mortem examinations revealed multifocal granulomatous and suppurative abscesses in the lungs and mesenteric lymph nodes. These postmortem indicators led the authors to suspect TB, and the PCR test and bacteriology tests confirmed it as an infection by the Mycobacterium bovis. This survey discusses the important implications of such findings for wildlife, especially livestock, as well as for human TB disease control, because deer are often conserved in public zoos and humans often come into contact with them. © 2016, Iranian Journal of Public Health. All rights reservsd. Source

Shahsavandi S.,Razi Vaccine & Serum Research Institute | Ebrahimi M.M.,Razi Vaccine & Serum Research Institute | Sadeghi K.,Razi Vaccine & Serum Research Institute | Mahravani H.,Razi Vaccine & Serum Research Institute
Virologica Sinica | Year: 2015

Influenza viruses continue to emerge and re-emerge, posing new threats for public health. Control and treatment of influenza depends mainly on vaccination and chemoprophylaxis with approved antiviral drugs. Identification of specific epitopes derived from influenza viruses has significantly advanced the development of epitope-based vaccines. Here, we explore the idea of using HLA binding data to design an epitope-based vaccine that can elicit heterosubtypic T-cell responses against circulating H7N9, H5N1, and H9N2 subtypes. The hemokinin-1 (HK-1) peptide sequence was used to induce immune responses against the influenza viruses. Five conserved high score cytotoxic T lymphocyte (CTL) epitopes restricted to HLA-A*0201-binding peptides within the hemagglutinin (HA) protein of the viruses were chosen, and two HA CTL/HK-1 chimera protein models designed. Using in silico analysis, which involves interferon epitope scanning, protein structure prediction, antigenic epitope determination, and model quality evaluation, chimeric proteins were designed. The applicability of one of these proteins as a heterosubtypic epitopebased vaccine candidate was analyzed. © 2015, Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg. Source

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