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Amiri A.,Shiraz University of Medical Sciences | Namavari M.,Razi Research Institute of Vaccine and Serum | Rashidi M.,Shiraz University of Medical Sciences | Fahmidehkar M.A.,Shiraz University of Medical Sciences | Seghatoleslam A.,Shiraz University of Medical Sciences
Asian Pacific Journal of Cancer Prevention | Year: 2015

Breast and colorectal cancers rank high in Iran as causes of mortality. Most of the current treatments are expensive and non-specific. The potential anticancer properties of common home gecko, Cyrtopodion scabrum, were investigated in this study. The effects of C. scabrum extract on proliferation, viability and migration of the colorectal cancer (SW-742), breast cancer (MCF-7) and normal (MSC) cell lines were investigated using MTT and in vitro wound healing assay. IC50 values calculated for the extract were 559±28.9μg/ml for MCF-7 and 339±11.3μg/ml for SW-742. No toxic effects on the normal control cells were observed. MCF-7 and SW-742 cell growth was inhibited by 32.6% and 62%, under optimum conditions, compared to the untreated control cells. The extract also decreased the motility and migration ability of both cancer cell lines, with no significant effects on the normal control cells. Data suggest C. scabrum extract as a useful natural resource for targeting cancer cells specifically.

Seghatoleslam A.,Shiraz University of Medical Sciences | Nikseresht M.,Yasuj University of Medical Sciences | Shafiee S.M.,Shiraz University of Medical Sciences | Monabati A.,Shiraz University of Medical Sciences | And 4 more authors.
Molecular Biology Reports | Year: 2012

The novel human gene, designated ubiquitinconjugating enzyme E2Q family member 1 (UBE2Q1) maps to chromosome 1q21.3. The gene has an open reading frame corresponding to 422 amino acids and contains a RWD domain and an E2 ubiquitin conjugating enzyme domain. Here, we investigated the expression levels of both mRNA and protein of UBE2Q1 gene in cancerous versus normal parts of breast specimens from 26 patients. Realtime PCR data showed that the relative expression level of UBE2Q1 mRNA was significantly greater in cancers than in non-cancerous tissues of breast specimens (Mean ± SEM, 0.064 ± 0.015 for cancers and 0.026 ± 0.01 for noncancerous tissues, P<0.05 Mann-Whitney test). A rabbit polyclonal antibody was generated against an amino acid sequence predicted from the DNA sequence of UBE2Q1 gene. This antibody was used to perform Western blotting on 21 cases in our cohort of breast specimens. Thus, 13 (61.904%) of the cases showed an increase in the UBE2Q1 immunoreactivity in their cancerous tissues as compared with the corresponding normal tissues. This result along with the real-time PCR data shows that the novel human gene, UBE2Q1, is expressed in human breast and may have implications for pathogenesis of breast cancer. ©Springer Science+Business Media B.V. 2011.

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