Development and validation of spectroscopic method for simultaneous estimation of salbutamol sulphate, Ambroxol hydrochloride and cetirizine hydrochloride in combined pharmaceutical tablet formulation: A novel technique for in-vitro dissolution studies
Sharma D.,Rayat Bahra Institute of Pharmacy |
Singh G.,Guru Nanak Institute of Pharmacy |
Kumar D.,Chandigarh Institute of Microbial Technology |
Singh M.,Quantum Solutions
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014
Objective: To develop a simple, rapid, accurate, precise and economical spectroscopic method for the simultaneous determination of Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine Hydrochloride in combined pharmaceutical tablet formulation and validate as per ICH guidelines.Methods: In this method, the 6.8 pH phosphate buffer was selected for the developing spectral characteristics of the three drugs. The overlay spectra of Salbutamol Sulphate, Ambroxol hydrochloride and Cetirizine hydrochloride were resolved by making the use of simultaneous equation method based on measurement of absorbance at three wavelengths.Results: The λmaxof Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine hydrochloride were found to be 276 nm, 244 nm and 230 nm. The method obeyed Beer Lambert’s law in the concentration range of 10-100 μg/ml for SAL, 2- 18 μg/ml for AMB HCl and 2-20 μg/ml for CET HCl. The high values of correlation coefficient (R) indicated good linearity of calibration plot for the drugs. Result of percentage recovery study confirms the accuracy of proposed method. Percentage RSD for precision and accuracy of the method was found to be less than 2%. LOD values for SAL, AMB, and CET were found to be 0.523 ððg/mL, 0.450 ððg/mL and 0.457 ððg/mL, respectively. LOQ values for SAL, AMB, and CET were found to be 1.372 ððg/mL, 1.424 ððg/mL and 1.386 ððg/mL, respectively.Conclusion: A rapid, economical, accurate, precise and reproducible simultaneous equation spectroscopic method was developed and validated. The proposed method can be employed for routine analysis of Salbutamol Sulphate, Ambroxol Hydrochloride and Cetirizine Hydrochloride in combined pharmaceutical tablet formulation. ©.2014, International Journal of Pharmacy and Pharmaceutical Science. All Right Reserved.
Singh S.,Panacea Biotec Pvt. Ltd. |
Mohan C.,Rayat Bahra Institute of Pharmacy
European Journal of Medicinal Chemistry | Year: 2014
Within the vast range of heterocycles, benzimidazole and its derivatives are found to be trendy structures employed for discovery of drugs in the field of pharmaceutical and medicinal chemistry. The unique structural features of benzimidazole and a wide range of biological activities of its derivatives made it privileged structure in drug discovery. Recently, benzimidazole scaffold has emerged as a pharmacophore of choice for designing analgesic and anti-inflammatory agents active on different clinically approved targets. To pave the way for future research, there is a need to collect the latest information in this promising area. In the present review we have collated published reports on this versatile core to provide an insight so that its full therapeutic potential can be utilized for the treatment of pain and inflammation. © 2014 Elsevier Masson SAS. All rights reserved.
Sachdeva R.K.,Rayat Bahra Institute of Pharmacy |
Kapoor G.,Lovely Professional University
International Journal of Pharma and Bio Sciences | Year: 2013
The present study investigates the effect of crystalline and amorphous carriers for the improvement of dissolution profile of broad spectrum antifungal agent Itraconazole. A comparative study was performed to estimate the ability of Poloxamer-407 (crystalline carrier) and modified Gum Karaya (amorphous carries) for enhancing the dissolution profile of Itraconazole (BCS class II). Solid dispersions prepared (using Poloxamer-407) by hot melt method, exhibited 92% drug release at the end of 2 hrs with good percent yield and percent drug content. While solid dispersions prepared (using modified gum karaya) by solvent evaporation method, exhibited 86% drug release at the end of 2 hrs but it possessed low drug content and low drug yield. Thus, complete amorphous state and absence of crystalline peaks of Itraconazole were observed with Poloxamer-407 while presence of small number of crystalline peaks with much reduced intensity (of Itraconazole) shifting towards lower melting endotherm were observed in case of modified gum karaya.
Verma S.,Rayat Bahra Institute of Pharmacy |
Jain A.K.,Guru Nanak Institute of Pharmacy
Artificial Cells, Nanomedicine and Biotechnology | Year: 2015
Human immunodeciency virus (HIV) infection has become devastating in last a few years. Nearly 7400 new infection cases are coming every day. Highly active antiretroviral therapy (HAART), which involves combination of at least three antiretroviral (ARV) drugs, has been used to extend the life span of the HIV-infected patients. HAART has played an important role to reduce mortality rate in the developed countries but in the developing countries condition is still worst with millions of people being infected by this disease. For the improvement of the situation, nanotechnology-based drug system has been explored for the HIV therapeutics. Nanosystems used for HIV therapeutics offer some unique advantage like enhancement of bioavailability, water solubility, stability, and targeting ability of ARV drugs. Main nanotechnology-based systems explored for HIV therapeutics are liposomes, nanoparticles, niosomes, polymeric micelles, and dendrimers. Present manuscript reviews conventional method of HIV therapeutics and recent advances in the eld of nanotechnology-based systems for treatment of HIV-AIDS. © 2014 Informa Healthcare USA, Inc.
Sharma S.,Guru Nanak Dev University |
Singh J.,Rayat Bahra Institute of Pharmacy |
Bedi P.M.S.,Guru Nanak Dev University
Recent Patents on Anti-Cancer Drug Discovery | Year: 2015
Protein kinases constitute one of the largest and most functionally diverse gene families that regulate key cell functions. In past several years, kinase inhibition has emerged as potential anti-cancer drug target. Purine is a priveleged heterocyclicnucleus which exists in the chemical architecture of various bioactive compounds. Numerous reports on the use of purine analogues in the treatment of acute leukemias (thiopurines, pentostatin), as antiviral (acyclovir, penciclovir, ganciclovir), as immunosuppressive (azathioprine), as antitumor (vidarabine), as bronchodilator (theophylline) have been revealed. In the past decade, purine analogues have emerged as significantly potent kinase inhibitors. A fair amount of research has been done and several patents have also been published highlighting the kinase inhibitory action of purines. Caffeine, 2- aminopurine, purvalanol-A, seleciclib, FSBA, adenosine thiol analogue possessing purine as the basic moiety fall under this category. In view of the use of purines for the inhibition of kinases, there is need for compilation of data specifying the prominence of purines in the treatment of cancer through this mechanism. The structure of the potent compounds, their IC50 values, models used and the enzymes/ receptors/ targets involved have been presented in this review. The present compilation covers the patents published entailing the purines as kinase inhibitors and the purine drugs employed in chemotherapy. © 2015 Bentham Science Publishers.
Formulation development and evaluation of fast disintegrating tablets of salbutamol sulphate, cetirizine hydrochloride in combined pharmaceutical dosage form: a new era in novel drug delivery for pediatrics and geriatrics
PubMed | Quantum Solutions, Rayat Bahra Institute of Pharmacy and Chandigarh Institute of Microbial Technology
Type: | Journal: Journal of drug delivery | Year: 2015
The objective of the present study was to prepare the fast disintegrating tablet of Salbutamol Sulphate, Cetirizine Hydrochloride in combined tablet dosage form for respiratory disorders such as bronchitis, asthma, and coughing for pediatrics and geriatrics. The tablets were prepared by direct compression technique. Superdisintegrant such as Sodium Starch Glycolate was optimized as 4% on the basis of least disintegration time. Different binders such as MCC and PVP K-30 were optimized along with optimized superdisintegrant concentration. 1% MCC was selected as optimum binder concentration on the basis of least disintegration time. The tablets were evaluated for hardness, friability, weight variation, wetting time, disintegration time, and drug content uniformity. Optimized formulation was further evaluated by in vitro dissolution test, drug-excipient compatibility, and accelerated stability study. Percent weight variation and content uniformity were within the acceptable limit. The friability was less than 1%. The wetting time and disintegration time were practically good for all formulations. FTIR studies and accelerated stability study showed that there was no interaction between the drug and excipients. It was concluded that, by employing commonly available pharmaceutical excipients such as superdisintegrants, hydrophilic and swellable excipients and proper filler, a fast disintegrating tablet of Salbutamol Sulphate, Cetirizine Hydrochloride in combined tablet dosage form, were formulated successfully with desired characteristics.
Sharma A.,Rayat Bahra Institute of Pharmacy |
Piplani P.,University Institute of Pharmaceutical science
Central Nervous System Agents in Medicinal Chemistry | Year: 2013
In view of the large libraries of acetylcholinesterase inhibitors (AChEIs) that are now being handled in organic synthesis, the identification of drug biological activity is advisable prior to synthesis and this can be achieved by employing predictive biological property methods. In this sense, Quantitative Structure-Activity Relationships (QSAR) or docking approaches have emerged as promising tools. The intention of this review is to summarize the present knowledge concerning computational predictions of AChEIs and AChE. © 2013 Bentham Science Publishers.
Poonia P.,Rayat Bahra Institute of Pharmacy |
Niazi J.,Rayat Bahra Institute of Pharmacy |
Chaudhary G.,Rayat Bahra Institute of Pharmacy |
Kalia A.N.,Director Herbal Drug Research
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011
Antioxidant activity of 90% methanol and aqueous extracts of Jasminum mesnyi leaves and total phenolic and flavonoids contents were investigated for free and peroxyl radical scavenging activity, reducing capacity by adopting in vitro models. DPPH scavenging activity showed IC50 of 90% methanol and aqueous extracts (25.27±0.6 μg/ml and 71.84±0.06 μg/ml respectively) when compared with ascorbic acid and rutin (8.84±0.05 and 3.78±0.153 μg/ml respectively). The reducing power of extracts increased in concentration dependent manner in FRAP method. The IC50 value of lipid peroxidation assay for 90% methanol, aqueous extracts and BHT were 84.69±0.008 μg/ml, 145.62±0.007 μg/ml, and 48.89±0.01 μg/ml respectively. 90% methanol extract of J. mesnyi leaves have more antioxidant potential in comparison to the aqueous extract and are at par with standard antioxidants in all assay techniques. The results obtained in the present study indicate that the leaves of J. mesnyi are a potential source of natural antioxidants.
PubMed | Rayat Bahra Institute of Pharmacy
Type: Journal Article | Journal: Central nervous system agents in medicinal chemistry | Year: 2013
In view of the large libraries of acetylcholinesterase inhibitors (AChEIs) that are now being handled in organic synthesis, the identification of drug biological activity is advisable prior to synthesis and this can be achieved by employing predictive biological property methods. In this sense, Quantitative Structure-Activity Relationships (QSAR) or docking approaches have emerged as promising tools. The intention of this review is to summarize the present knowledge concerning computational predictions of AChEIs and AChE.
PubMed | Quantum Solutions, Rayat Bahra Institute of Pharmacy, Chandigarh Institute of Microbial Technology and CT Institute of Pharmaceutical science
Type: | Journal: Journal of pharmaceutics | Year: 2015
Recent developments in fast disintegrating tablets have brought convenience in dosing to pediatric and elderly patients who have trouble in swallowing tablets. The objective of the present study was to prepare the fast disintegrating tablet of Cetirizine Hydrochloride for allergic and respiratory disorders. As precision of dosing and patients compliance become important prerequisite for a long-term treatment, there is a need to develop a formulation for this drug which overcomes problems such as difficulty in swallowing, inconvenience in administration while travelling, and patients acceptability. Hence, the present investigation was undertaken with a view to develop a fast disintegrating tablet of Cetirizine Hydrochloride which offers a new range of products having desired characteristics and intended benefits. Superdisintegrants such as Sodium Starch Glycolate were optimized. Different binders were optimized along with optimized superdisintegrant concentration. The tablets were prepared by direct compression technique. The tablets were evaluated for hardness, friability, weight variation, wetting time, disintegration time and uniformity of content. Optimized formulation was evaluated by in vitro dissolution test, drug excipient compatibility and accelerated stability study. It was concluded that fast disintegrating tablets of Cetirizine Hydrochloride were formulated successfully with desired characteristics which disintegrated rapidly, provide rapid onset of action, and enhance the patient convenience and compliance.