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Volkova S.A.,RAS Pacific Institute of Bioorganic Chemistry
Turczaninowia | Year: 2016

The leaf epidermis in Conioselinum longifolium and C. tataricum tribe Ligusticeae family Apiaceae has been studied. The plants have hypostomatous leaves, and anomocytic, anisocytic, gemiparacytic, gemidiacytic of stomatal apparatus identified. Sometimes there are diacytic, paracytic and tetracytic stomatotypes. The prevailing stomatal apparatus type is anomocytic. There are several types of stomata on the one leaf at the same time. The main diagnostic features in the studied species are: the number of cells and stomata per unit area, the shape and character of the outline of the epidermal cells and the number of cells adjacent to the stomata. Both types of stomatas surrounded by 3-6 cells. The most common type is anomocytic stomata surrounding 4 cells. Anomocytic type compiles 75 % of the total number of the stomatas, and C. tataricum has 90 % of the stomatas this type. Stomatal complexes with 6 neighboring cell are very rary. The number of the stomatas and cell per 1 mm2 in C. longifolium is about 1.5 times greater than in C. tataricum. Outlines of the leaf epidermal cells on the adaxial surface of C. longifolium are straightforward rounded. C. tataricum has a large-winding wall of the cells. There are twisting and waving outlines of the epidermal cell on the abaxial side in C. longifolium, and large-twisting in C. tataricum. Stomatal index differs insignificantly. The variability of the characteristics of the leaf epidermis is less average.


Kim S.B.,Chungnam National University | Nedashkovskaya O.I.,RAS Pacific Institute of Bioorganic Chemistry
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

A novel strain, designated KMM 6019T, was isolated from coastal seawater and subjected to taxonomic examination using a polyphasic approach. A comparative analysis of 16S rRNA gene sequences revealed that strain KMM 6019T formed a distinct phyletic line within the genus Winogradskyella, a member of the family Flavobacteriaceae, phylum Bacteroidetes. The 16S rRNA gene sequence similarity between the novel isolate and the type strains of the recognized species of the genus Winogradskyella was 94.0-97.8 %. Winogradskyella thalassocola KMM 3907T was the closest relative, with 97.8% sequence similarity. The predominant fatty acids of strain KMM 6019 T were iso-C15 : 0, iso-C15 : 1, anteiso-C 15 : 0, iso-C15 : 0 3-OH, iso-C16 : 0 3-OH, C15 : 0, iso-C17 : 0 3-OH and summed feature 3 comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c, which are characteristic for members of the genus Winogradskyella. The DNA G+C content was 35.3 mol%. Strain KMM 6019T moved by gliding and grew with 1-5% NaCl and at 4-30 °C. The novel strain degraded gelatin, casein and starch and produced acid from cellobiose, D-glucose and maltose. Strain KMM 6019 T could clearly be differentiated from the other Winogradskyella species by the ability to utilize inositol and sorbitol and to produce hydrogen sulphide. On the basis of phylogenetic and phenotypic findings, strain KMM 6019T is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella pacifica sp. nov. is proposed. The type strain is KMM 6019T (=KCTC 22997T=LMG 22568T). © 2010 IUMS.


Bulgakov V.P.,Russian Academy of Sciences | Inyushkina Y.V.,Russian Academy of Sciences | Fedoreyev S.A.,RAS Pacific Institute of Bioorganic Chemistry
Critical Reviews in Biotechnology | Year: 2012

Rosmarinic acid (RA) is one of the first secondary metabolites produced in plant cell cultures in extremely high yields, up to 19% of the cell dry weight. More complex derivatives of RA, such as rabdosiin and lithospermic acid B, later were also obtained in cell cultures at high yields. RA and its derivatives possess promising biological activities, such as improvement of cognitive performance, prevention of the development of Alzheimer's disease, cardioprotective effects, reduction of the severity of kidney diseases and cancer chemoprevention. The TNF-α-induced NF-κB signaling pathway has emerged as a central target for RA. Despite these impressive activities and high yields, the biotechnological production of these metabolites on an industrial scale has not progressed. We summarized data suggesting that external stimuli, the Ca2-dependent NADPH oxidase pathway and processes of protein phosphorylation/dephosphorylation are involved in the regulation of biosynthesis of these substances in cultured plant cells. In spite of growing information about pathways regulating biosynthesis of RA and its derivatives in cultured plant cells, the exact mechanism of regulation remains unknown. We suggest that further progress in the biotechnology of RA and its derivatives can be achieved by using new high-throughput techniques. © 2012 Informa Healthcare USA, Inc.


Evtushenko E.V.,RAS Pacific Institute of Bioorganic Chemistry
Carbohydrate Research | Year: 2012

Benzoylation of methyl and benzyl glycopyranosides by benzoic anhydride in acetonitrile in the presence of copper(II) trifluoroacetate as a promoter has given monobenzoates with a good yield and high regioselectivity. The composition of monobenzoates depended both on a configuration of hydroxyl groups and on a configuration of aglycone. The simple syntheses of the monobenzoates of some glycosides are offered. © 2012 Elsevier Ltd. All rights reserved.


Santalova E.A.,RAS Pacific Institute of Bioorganic Chemistry
Natural Product Communications | Year: 2012

Treatment of 3,5-dibromoverongiaquinol (1) with NaHCO3-MeOH at room temperature gave cavernicolin-1 (2), cavernicolin-2 (3), the 4,7-dimethoxy analogue of subereatensin (4) and dimethyl ketal (5). These transformations may clarify the origin of the cavernicolins and subereatensin isolated from the extracts of some verongid sponges.


Ivanchina N.V.,RAS Pacific Institute of Bioorganic Chemistry | Kicha A.A.,RAS Pacific Institute of Bioorganic Chemistry | Stonik V.A.,RAS Pacific Institute of Bioorganic Chemistry
Steroids | Year: 2011

Structures, taxonomic distribution and biological activities of steroid glycosides isolated from marine organisms over the last 8-10 years are reviewed. The bibliography includes 130 references. © 2010 Elsevier Inc.


Utkina N.K.,RAS Pacific Institute of Bioorganic Chemistry
Natural Product Communications | Year: 2013

Antioxidant activities of zyzzyanones A-D (1-4) and makaluvamines C (8), E (5), G (6), H (9), and L (7) isolated from the marine sponge Zyzzya fuliginosa (Carter, 1879) were evaluated using 2,2'-azinobis(3-ethylbenzothiazoline-6- sulfonate) (ABTS) scavenging assay and AAPH (2,2'-azobis(2-amidinopropane) dihydrochloride) induced autoxidation of linoleic acid. Zyzzyanones A, B (1, 2; TE = 0.3), C, D (3, 4; TE = 0.24) and makaluvamines 5-7 (TE = 0.6) displayed moderate activities in the ABTS assay and in the autoxidation of linoleic acid (61-66 % inhibition at concentrations of 0.1 mM). Makaluvamines C (8) and H (9) were essentially inactive in the both assays. Structure-activity relationships showed that antioxidant activities of tested compounds depended on the presence of a phenolic function in molecules. Makaluvamines 5-7 possessing a p-hydroxystyryl moiety were more active than zyzzyanones 1-4 possessing a p-hydroxyphenyl fragment. The presence of a charged side chain in 1 and 2 slightly increases their ABTS scavenging activity in comparison with compounds 3 and 4. Structural variations in a pyrroloquinoline skeleton of 5-7 and in a dipyrroloquinone core of 1-4 have no effect on activities.


Evtushenko E.V.,RAS Pacific Institute of Bioorganic Chemistry
Journal of Carbohydrate Chemistry | Year: 2015

Benzoylation of 4,6-O-benzylidene acetals of glycopyranosides by benzoic anhydride in acetonitrile in the presence of Cu(CF3COO)2 as a promoter gave 2-benzoates for α-D-glucopyranosides and α-D-mannopyranosides and 3-benzoates for β-D-galactopyranosides in good yields with high regioselectivity. Benzoylation of 4,6-O-benzylidene acetals of glycopyranosides of D-galactose and D-mannose by benzoyl chloride in the presence of MoO2(acac)2 as a catalyst in all studied cases led to regioselective 3-substitution. © 2015 Taylor and Francis Group, LLC.


Nedashkovskaya O.I.,RAS Pacific Institute of Bioorganic Chemistry
Archives of microbiology | Year: 2014

A Gram-negative, aerobic, rod-shaped, motile by gliding and yellow-pigmented bacterium, designated strain 10Alg 130(T), that displayed the ability to destroy polysaccharides of red and brown algae, was isolated from the red alga Ahnfeltia tobuchiensis. The phylogenetic analysis based on 16S rRNA gene sequence placed the novel strain within the genus Flavobacterium, the type genus of the family Flavobacteriaceae, the phylum Bacteroidetes, with sequence similarities of 96.2 and 95.7 % to Flavobacterium jumunjiense KCTC 23618(T) and Flavobacterium ponti CCUG 58402(T), and 95.3-92.5 % to other recognized Flavobacterium species. The prevalent fatty acids of strain 10Alg 130(T) were iso-C15:0, iso-C15:0 3-OH, iso-C17:0 3-OH, C15:0 and iso-C17:1ω9c. The polar lipid profile consisted of phosphatidylethanolamine, two unknown aminolipids and three unknown lipids. The DNA G+C content of the type strain was 34.3 mol%. The new isolate and the type strains of recognized species of the genus Flavobacterium could strongly be distinguished by a number of phenotypic characteristics. A combination of the genotypic and phenotypic data showed that the algal isolate represents a novel species of the genus Flavobacterium, for which the name Flavobacterium ahnfeltiae sp. nov. is proposed. The type strain is 10Alg 130(T) (=KCTC 32467(T) = KMM 6686(T)).


Evtushenko E.V.,RAS Pacific Institute of Bioorganic Chemistry
Journal of Carbohydrate Chemistry | Year: 2010

Benzoylation of methyl and benzyl glycopyranosides by benzoyl chloride in the presence of MoO2(acac)2 as a catalyst resulted in 3-benzoates with good yield and high regioselectivity. Simple synthesis of the monobenzoates of some glycopyranosides is suggested. Copyright © Taylor & Francis Group, LLC.

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