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Jat B.L.,Maharana Pratap University of Agriculture and Technology | Sharma P.,Maharana Pratap University of Agriculture and Technology | Sharma P.,Rapeseed Mustard Research ICAR | Gour H.N.,Maharana Pratap University of Agriculture and Technology
Proceedings of the National Academy of Sciences India Section B - Biological Sciences | Year: 2013

Colletotrichum gloeosporioides was found to be associated with banana fruit rot. The fungus produces pectinolytic (Polygalacturonase trans eliminase, Pectin trans eliminase, Polygalacturonase and Pectin methyl esterase) and cellulolytic (cellulase) enzymes in vitro. Hence their involvement in degradation of fruits becomes obvious. Culture filtrate of C. gloeosporioides produced maximum fruit rotting in cork wounding (5.0) followed by carborundum rub method (4.0). Maximum Polygalacturonase trans eliminase (85.73 %) and Pectin trans eliminase (71.43 %) activity was recorded at 30 min time interval while Polygalacturonase was at par with 25 and 30 min (85.73 and 85.33 %, respectively). Cellulase activity was similar at both 25 and 30 min (58.33 %) time intervals. © 2012 The National Academy of Sciences, India.

Thakur A.K.,Himachal Pradesh University | Thakur A.K.,Rapeseed Mustard Research ICAR | Aggarwal G.,Himachal Pradesh University | Srivastava D.K.,Himachal Pradesh University
National Academy Science Letters | Year: 2012

The present studies describe the development of transgenic Himalayan poplar (Populus ciliataWall.) plants via Agrobacterium-mediated antisense cinnamyl alcohol dehydrogenase (CAD) gene transfer for genetic modification of lignin biosynthetic pathway for lowering down the lignin content by post-transcriptional silencing of CAD gene. Agrobacterium tumefaciens strain C58C1rifR(pMP90) harboring CAD gene in antisense orientation and npt-II gene in a binary vector p35SASCAD was used for genetic transformation experiments. By combining the best treatments of 48 h preculturing and 48 h co-cultivation time periods, a transformation frequency of 14% was obtained from petiole explant of P. ciliata. Out of total 9 randomly selected putative transgenic plants of P. ciliata, five were found to show the presence and integration of npt-II gene in poplar genome as confirmed by PCR using npt-II gene specific primers. npt-II gene which is transferred along with antisense CAD gene (in the same gene construct), got amplified with gene specific primers at 0.7 Kb. The lignin content of transgenic plants of P. ciliata was found to be reduced by a fraction of 3-4%as compared to the control plants after 4 months of transfer to the greenhouse conditions. © 2012 The National Academy of Sciences India.

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