News Article | May 17, 2017
Chino Basin Water Conservation District unveiled its new Inland Valley Garden Planner website (https://inlandvalleygardenplanner.org/) at an evening garden party in the Water Conservation Garden on May 11. The new garden planning website helps Inland Empire gardeners select and learn about the best plants for the region to create the “have-it-all” garden they want. Through stunning photos and an easy-to-navigate site, the Inland Valley Garden Planner offers free and detailed, regionally-specific information for gardeners in the Inland Empire area. Users can create their own profiles, save project lists, and easily save and print information on their selected or custom plant palettes, choosing from a curated list of over 350 plants that thrive in the Inland Empire. “The Inland Empire’s Mediterranean climate gives us so many incredible options for our gardens,” said Scott Kleinrock, CBWCD’s Conservation Programs Manager. “We truly can have it all in our outdoor spaces, with color, comfort, wonderful scents, and habitat for birds and pollinators, year-round, and without needing to water much, if we choose the right plants and put them in the right places.” New visitors to the site who create free accounts before June 30 will be entered into a raffle for a chance to win a family membership to Rancho Santa Ana Botanic Garden or gift certificates to the Grow Native Nursery. The site also provides cohesive pre-designed plant palettes and helpful lists for common conditions including slopes and small spaces. “We can have so much more than gravel and cactus or lawns that really don’t do much for us,” Kleinrock said. Developed by landscape architect and author Robert Perry for CBWCD, the Inland Valley Garden Planner reflects his decades of research into the best landscape plants for our region and uses his extensive and inspiring photo archive. “We constantly get questions from community members who want to save water in their landscapes while having beautiful, livable outdoor spaces to enjoy with their family and friends,” says Becky Rittenburg, CBWCD Community Programs Manager. “We launched the Inland Valley Garden Planner to provide Inland Empire homeowners with a free resource to help achieve their garden goals.” CBWCD works to sustain the regional water supply through public stewardship, stormwater percolation, demonstration, and education. Stop by the Water Conservation Center and demonstration garden in Montclair and see firsthand how beautiful, functional, and beneficial water conservation can be. The center is free and open to the public Monday through Saturday from 8 a.m. to 5 p.m.
Agency: NSF | Branch: Standard Grant | Program: | Phase: Systematics & Biodiversity Sci | Award Amount: 251.70K | Year: 2011
Loeselia is an obscure and little studied lineage in the flowering plant family Polemoniaceae (phlox family), which includes two genera: Loeselia L. and Dayia J.M. Porter. Despite the lack of detailed knowledge about the lineage, Loeselia has played a pivotal role in hypotheses concerning the origin of temperate members of Polemoniaceae from tropical ancestors. This research program will provide a better understanding of Loeselia (with 22 or more species). Preliminary studies of comparative DNA sequences provide evidence that Loeselia is composed of two groups of species based on ancestry, corresponding to Loeselia L. and Dayia J.M. Porter. Exhaustive taxon sampling within Loeselia and dense sampling of related groups will be used to develop an understanding of genealogical relationships (phylogeny) within and among species, using chloroplast and nuclear DNA sequences, morphological, and anatomical data.
Evidence generated through this research will lead to a new classification system and the first comprehensive monographs of Loeselia, including keys, descriptions, illustrations, distribution maps, and on-line resources. The studies of character evolution, using the same data, will provide a model for analyses of variation in reproductive traits such as self-incompatibility and floral symmetry, which is thought to be an important generator of flowering plant diversity.
Agency: NSF | Branch: Standard Grant | Program: | Phase: | Award Amount: 403.89K | Year: 2012
Parasitic plants obtain nutrients from host plants via a unique organ (the haustorium) and, thus, may negatively impact the survival and reproductive success of the host. Many parasites are completely dependent on their host, having lost the ability to make their own food through photosynthesis. This proposal focuses on a poorly known and diverse lineage of tropical parasitic plants through field studies in diversity hotspots on three continents. Variation in morphology and host preference will be documented and DNA sequence data will be used to assess evolutionary relationships. This evolutionary framework will be used to create a revised classification system and to investigate transitions in modes of parasitism, such as the loss of photosynthetic capability.
This project investigates a plant lineage which includes parasites that infest crops, resulting in annual losses worth several billion $US. An introduction of witchweed (Striga asiatica) was so damaging to the US corn crop that most tropical parasites are classified as noxious weeds. However, USDA agents have no means of differentiating a dangerous parasite from a harmless one. This project will provide an important online resource to assist border agents, farmers, and researchers in identifying noxious parasites wherever they occur, whether at points of entry into the US or in their native ranges.
Agency: NSF | Branch: Standard Grant | Program: | Phase: MAJOR RESEARCH INSTRUMENTATION | Award Amount: 165.05K | Year: 2016
An award is made to Rancho Santa Ana Botanic Garden (RSABG) to acquire modern instrumentation and analytical capacity for the Scanning Electron Microscopy (SEM) laboratory. SEM is one of the principal methods for examination of detailed surface structure (micromorphology) of biological materials. This instrument is of fundamental importance to research and training at RSABG, the largest botanic garden dedicated to California native plants and an important research center for plant comparative biology. Specifically, RSABG will acquire a Hitachi SU3500S Variable Pressure SEM, together with specimen preparation equipment and SEM-image analysis software. Vastly improved SEM capabilities will support research by RSABG faculty, staff scientists and students to further our understanding of the evolution of plant diversity through integrating comparative morphological and anatomical research on plants with cutting-edge molecular methods and phylogenetic analysis. The enhanced view of meristems, surfaces of leaves and stems, floral structures, seeds and pollen grains enabled by SEM imaging expands our knowledge of plant form and function. Notably, for many plant groups, SEM photomicrographs of pollen grains are essential components of species descriptions. In addition, quality SEM micrographs are an important means of effective and exciting visual communication, both in scientific presentations and in formal and informal education. SEM imagery from the Hitachi SEM will enhance our work in plant conservation through enhancing our ability to observe reproduction in rare plants. It will also contribute to public and formal education by providing images that are both beautiful and information-packed to support programming for K-6 and college students, as well as other visitors to RSABG through use of such images in exhibits. The facility will also augment microscopy capabilities available to researchers at the Claremont Colleges.
The SU3500S Variable Pressure Scanning Electron Microscope, the Cressington 108 Auto Sputter Coater with an MTM-20 high resolution thickness controller and MountainsMap Universal software represent significant improvements to and expansion of research infrastructure at RSABG. This integrated system will dramatically improve our conventional (i.e., high vacuum) SEM capabilities and also significantly expand our research tool chest by adding (1) higher resolution and magnification than currently achievable, (2) low vacuum capacity and a Peltier cooling stage for observing hydrated, living tissue and (3) sophisticated image analysis software. The low vacuum capabilities of the instrument will expand considerably the research questions that we can address while also ensuring that observations made are accurate. The image analysis software that is a vital part of the package will transform our analytical power to gather data from SEM images: to characterize, quantify and analyze 2D and 3D structure, and to integrate these morphometric data with comparative phylogenetic analyses to advance our knowledge of the patterns of plant evolution.
Agency: NSF | Branch: Standard Grant | Program: | Phase: PHYLOGENETIC SYSTEMATICS | Award Amount: 295.62K | Year: 2010
Irises originated in the Northern Hemisphere and consist of about 260 species that have colorful multidimensional flowers. This proposal focuses on understanding the evolution of a large group within Irises, a group that is morphologically diverse with plants ranging from tall to dwarf species. The evolution of this group will be studied by sampling the morphological and biogeographical diversity within the focal group. In addition, relationships among Iris species will be assessed using genetic data. The evolution of important vegetative and floral characters will be examined as a result of clarifying the evolutionary relationships among Iris species. In addition, this project will conduct developmental studies to decipher the pattern of formation of the elaborate, tri-dimensional, flowers.
This group of plants are of economic importance and are commonly used in public and private gardens. In many regions of the world Iris species are rare in nature leading several nations to protect all species. This project will train graduate and undergraduate students; in addition an international student will be trained in molecular genetic techniques. Seminars on this research will be given to the general public and horticultural groups.
Agency: NSF | Branch: Standard Grant | Program: | Phase: PHYLOGENETIC SYSTEMATICS | Award Amount: 18.51K | Year: 2015
Polyploidization (whole genome duplication) is, and likely was, instrumental in the diversification of flowering plants. Understanding how and why polyploidy occurs in flowering plants may provide critical insight into the interplay of adaptation and historic environmental changes like glaciation, volcanism, and global climate change. This research will provide an improved understanding of diversification in the Claytonia lanceolata species complex, which is critical for effective conservation and management of the habitats in which they grow. Cutting edge biochemical and computational techniques now allow us to better estimate evolutionary histories, and test hypotheses concerning the ecological, temporal, and geographic context of speciation. This research will increase our understanding of gene dispersal via seeds and pollen, gene sharing among species, and the influence of chromosome number variation on plant diversification. A better understanding of relict alpine communities in California will be an additional, important contribution. Scientific communication, public outreach and mentoring undergraduate students are integral parts of the project as well.
Polyploidy, potentially giving rise to species complexes, represents one of the fundamental processes in diversification and polyploids are important elements of plant communities across the globe. Preliminary study indicates that the Claytonia lanceolata species complex (Montiaceae), as currently circumscribed, is non-monophyletic and consists of many polyploid lineages inhabiting a variety of unique spatial and ecological conditions in the mountains of western North America. This project involves research into biogeography, chromosome evolution, niche diversification, and phylogeny of the C. lanceolata species complex and close relatives. The project investigates (1) abiotic and biotic habitat characteristics in the field to better understand mechanisms responsible for reproductive isolation, (2) chromosome number within and among populations using flow cytometry and chromosome counting methods to characterize potentially adaptive variation, (3) variation in vegetative characters using morphometrics to better develop hypotheses concerning selection acting on leaf and subterranean stem anatomy and morphology, and (4) patterns of inter-specific gene flow in sympatric populations using Next Generation Sequencing (RADseq) to understand the extent to which species are reproductively isolated. This research will result in a significant revision of our knowledge of species boundaries and their distributions in the C. lanceolata complex, in addition to the description of several taxa new to science.
Agency: NSF | Branch: Continuing grant | Program: | Phase: Biodiversity: Discov &Analysis | Award Amount: 309.98K | Year: 2014
This project combines new approaches and methods to understand evolutionary changes in flower color in a group of plants, Ruellia (Acanthaceae), with 200+ species in the Americas. The focus is on anthocyanins (the blue to red pigments that are the most important contributors of color in flowers). Cutting edge DNA sequencing approaches will be used to study the plant biochemical pathways that produce anthocyanins. These results will be placed in an evolutionary context to understand what happens when, for example, purple flowers evolve from red-flowered ancestors. The phylogeny or family tree of Ruellia will be reconstructed using novel molecular markers to be developed for this project; these markers will subsequently be useful to numerous other researchers.
Flowers are among the most common means by which people connect to nature: floral biology has great potential to engage broad audiences in science. This research is also important because it will shed light on the anthocyanins biochemical pathway. Anthocyanins contribute to numerous plant functions (e.g., pollinator and fruit disperser attraction, UV sunscreen, herbivore defense), and have also been linked to human health benefits (e.g., anti-cancer, -viral, -inflammatory, -arteriosclerosis activity; treatments for hypertension, vision disorders). Anthocyanins are common constituents of human diets, being found in frequently consumed fruits and vegetables. This research project will add substantially to knowledge of the genetic basis of anthocyanin production and will thus have implications for human health research.
Agency: NSF | Branch: Standard Grant | Program: | Phase: Systematics & Biodiversity Sci | Award Amount: 10.27K | Year: 2012
Species in genus Pyrola section Scotophylla (part of the Heath family) grow in western North America and possess several intriguing life history attributes that vary among species and will be investigated for this study. These include a special strategy for retrieving nutrition directly from fungi (i.e., mycoheterotrophy), leaves that range from large and photosynthetic to minute and non-functional, and flowers that are buzz-pollinated by bumble bees yet retain the ability to self-pollinate in the absence of bees. At least two findings complicate our general understanding of how species in section Scotophylla achieved (and maintain) reproductive isolation. First, the identification of cryptic species within the complex suggests that different genetic lineages have converged on a very similar form. Second, hybridization among species has been detected at low frequencies in populations where species co-occur. In order to understand how gene flow relates to speciation and morphological diversity, this study focuses on the phylogeography (i.e., mapping geographic and genetic lineage histories) of section Scotophylla using DNA sequence information from plants across revised species ranges. Additionally, this study focuses on (1) understanding how mixed-mating systems contribute to gene flow and species diversity, (2) determining the causes of breakdown in reproductive isolation, and (3) understanding how timing in development has played a role in the evolution of leaf morphology, anatomy, and function in this mycoheterotrophic plant lineage.
This study will provide a revision of geographic ranges for species in section Scotophylla, the description of a new, cryptic species, and a contribution to ongoing discourse concerning how the recognition of cryptic species affects our interpretation of biological diversity. As fundamental, symbiotic elements of mature forest communities, mycoheterotrophic taxa like Pyrola serve effectively as indicators of ecosystem health, ultimately making ecology and evolution more accessible to a range of audiences. Understanding the evolutionary history of Pyrola lineages through time may ultimately change peoples? perceptions of both natural history and ongoing efforts by conservation biologists in western North America. Findings from this study will be available in peer-reviewed journals, at scientific conferences and in non-scientific forums, and as interpretive literature for students and teachers interested in natural history.
Agency: NSF | Branch: Standard Grant | Program: | Phase: | Award Amount: 14.99K | Year: 2012
The flowering plant family Sabiaceae comprises three genera and about 150 species of trees and shrubs that are distributed throughout the Neotropics and Southeast Asia. This group has not been subject to modern evolutionary research, and thus questions remain about the relationships among its members, as well as about the placement of the family among flowering plants. The main goal of this study is to generate a phylogenetic framework across taxonomic levels that will answer these questions and also allow the study of other aspects of the biology of the group, such as biogeography and the evolution of reproductive characters. To achieve this, modern methods, such as the comparison of gene sequences and phylogenetic dating using fossil information, will be used.
This study will generate data that will improve our understanding of a recalcitrant part of the angiosperm tree of life. Furthermore, this study will test biogeographic hypotheses that have been proposed for other groups of plants with similar disjunct distributions, and fossil-based dating will provide geological timeframes in which the divergence and evolution of the family took place.
Agency: NSF | Branch: Continuing grant | Program: | Phase: BIOLOGICAL RESEARCH COLLECTION | Award Amount: 126.39K | Year: 2016
Student Engagement in the Preservation of Historically Significant Collections at Rancho Santa Botanic Garden
Natural history collections offer a lens into the past and a means to envision the future. Collections are especially important in predicting biodiversity change with shifts in climate and land use. The Herbarium at Rancho Santa Ana Botanic Garden (RSABG) houses more than 1.2 million specimens and is heavily utilized for research, education, and public outreach. From multiple sources, the Herbarium acquired 30,000 plant specimens collected by ten significant 20th century botanists. 70% of these were collected from 15 of 34 designated world biodiversity hotspots; 30% represents the California Floristic Province biodiversity hotspot. These specimens are in critical need of curation owing to poor storage conditions. Several specimens have been identified as critical for describing and naming species new to science. Curation of these collections will provide physical access to collections currently unavailable for study, promote discovery of species new to science, and yield new distribution and phenology data. Importantly, curation will ensure critically needed preservation. Digitization efforts will provide access of this rich source of specimen data to researchers, students, and the public. Integral to the project are activities involving the participation of graduate students, undergraduates, and high school students. Notably, RSABG will expand on a successful undergraduate internship program by engaging underserved youth from the Greater Los Angeles metropolitan area. Especially targeted are at-risk and transitional aged youth at the high school level, who will be trained in a six-week summer junior intern program. All interns will participate in five workshops that will serve to connect students to collections, natural history, and biodiversity. An exhibit at RSABG will highlight the activities and student participation in the project.
As herbaria serve expanding needs for research, education, and conservation management, it is critical that collections of historic, taxonomic, and geographic significance are curated and digitized, ensuring their availability for research through investments in their long-term security. Through various sources, the Herbarium at Rancho Santa Ana Botanic Garden acquired 30,000 vascular plant specimens of historic, taxonomic, and geographic importance representing the collections of ten significant botanists of the 20th century. These specimens are in danger of deterioration, owing to unsatisfactory past and current storage conditions (e.g., non-archival newspapers). Thus, these collections are in critical need of curation and improved storage to secure them for posterity. Several specimens have been identified as type material that was thought to have been lost. The principal objective of this three-year project is to curate and digitize these specimens through student participation. A large cadre of students at different levels in their academic career will participate in all aspects of the project, including specimen preparation, databasing, imaging, and georeferencing. Specimen data will become available for more than 18,000 species of vascular plants in ~350 families. All data resulting from this project will be shared with iDigBio, GBIF, the Consortium of California Herbaria, and the RSABG Herbarium web portal. Additional information about RSABG, its outreach activities, and the results of this project is available at http://www.rsabg.org.