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Ranchi, India

Rani S.,Ranchi Womens College | Srivastava R.,Jn College | Gupta D.K.,Kcb College
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2013

Vermicomposting of vegetable wastes amended with cattle manure using earthworm species Eisenia foetida was studied for a period of 45 days. The vegetable waste mixture had 45.15 ± 1.9% organic carbon, 1.5 ± 0.21% total nitrogen and 30.1 ± 2.1 C: N ratio initially. Organic carbon and C/N ratio decreased in both the control and experimental pots whereas nitrogen content increased during the study period. Significant variation in total organic carbon, nitrogen content and C/N ratio (P<0.05) was observed on 15th, 30th and 45th day of composting. © Global Science Publications.

Mehta S.,Ranchi Womens College
Vegetos | Year: 2013

Aloe vera L. is an economically important plant. In nature its regeneration is very slow. Therefore to fulfill its demand at commercial level it is necessary to use in-vitro propagation for rapid plant production. Leaf and shoot explants of Aloe Vera L. cultured on Murashige & Skoog’s medium (1962) supplemented with NAA, BAP and IBA alone or in combination. Development of root and callus were investigated from excised leaf. © 2013, Society for Plant Research. All rights reserved.

Roy S.,Ranchi Womens College | Mehta A.,Ranchi Womens College | Rani Mishra R.,SASTRA University
Vegetos | Year: 2013

Microbial enzymes meet industrial demands. Over 60% of the total Phosphorus in cereal grains and oil seeds as well as their by-products is found as phytate, myo-inositol hexakisphosphate. Phytases are a group of enzymes that initiate the phosphate hydrolysis from phytate and produce myo-inositol and inorganic phosphate by catalyzing the stepwise removal of the phosphate groups. Microbial phytases effectively improve dietary phytate-P utilization. Extracellular phytase produced by Bacillus subtilis ATCC 6051 was purified by acetone precipitation, DEAE- sepharose and phenylsepharose column chromatographies. The molecular weight of the enzyme was estimated to be 48 kDa on gel filtration and 43kDa on SDS-polyacrylamide gel electrophoresis. Its optimum pH and temperature for phytase activity were pH 6.2 to 8.2 and 40°C without 10mM CaCl2 and pH 6.5 to 9.5 and 60° with 10mM CaCl2. The enzyme activity was stable from pH 6.5to 10. The enzyme had an isoelectric point of 6.8. It was very specific for sodium phytate. The Km value for sodium phytate was 50μM. Its activity was inhibited by EDTA and metal ions such as Mn2+,Hg2+,Cu2+,Cr3+, Co2+,Ba2+and Cd2+ions. The enzyme has great potential in food industry, probiotics, animal feed supplement and transgenic crops.

Pandey M.,Ranchi Womens College | Srivastava P.K.,Ranchi Womens College | Bhattacharjee V.,Birla Institute of Technology
International Journal of Engineering and Technology | Year: 2015

Fuzzy approach is based on premise that the key elements in human thinking are not just numbers. Decision maker's response to the different alternatives and also preferences to the various attributes may be sometimes expressed in linguistic variables.

Purak I.,Ranchi Womens College | Mehta A.,Ranchi Womens College
Vegetos | Year: 2011

Cardiospermum halicacabum Linn. belonging to family Sapindaceae is commonly known as Balloon Vine due to inflated membranous balloon like tri-celled ridged fruits having black seeds with prominent white heart shaped scar. It is recognized as a medicinal plant of repute in Ayurvedic and Homoeopathic mode of treatment under different names such as Jyotishmati, Kanphuti, Lataphatkari, Indravalli, Heart pea, Love- in-a -puff, Heart seed, Winter cherry etc. The plant possesses active ingredients viz. alkaloids, flavonoids, proanthocyanide, cyanolipids, glycosides, saponins, tri-terpenes, steroids ec. It is used to cure rheumatism, diarrhoea, chronic bronchitis, nervous diseases, stiffness of limbs, snake bite, pulmonary troubles, itchy skin, problems in menstrual cycle, gastric ulcer, earache, eyesore and piles. Standardization of protocol was undertaken for achieving callus mass from different parts of Cardiospermum halicacabum. Different explants responded differently to chemicals and media they had been subjected.MS Medium supplemented with low concentration of 2,4 -D had a tendency to regenerate into shoot and complete plantlet but high concentration induced callus formation as well as proliferation. Green embryogenic callus from leaf explants was observed in media containing 2,4-D. Callus tissue is good source of genetic and karyotypic variability so variants can be regenerated from these genetically variable cells. Callus culture is useful for obtaining commercially important secondary metabolities. Several biochemical assays can be performed from callus culture.

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