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Ranchi, India

Allu P.K.R.,Indian Institute of Technology Madras | Chakraborty B.,Ranchi University | Das M.,University of Calcutta | Mahapatra N.R.,Indian Institute of Technology Madras | Ghosh S.,Ranchi College
Aquaculture International | Year: 2014

High consumer preference together with its polyculture potential has undoubtedly driven Rohu (Labeo rohita) and Catla (Catla catla) to top the list of the most preferred fishes among the Indian major carps. Commonly found in these fishes are hybrids that can be natural or man-made. Increasing emphasis on biodiversity issues has necessitated proper stock management of these through molecular genetics techniques. Also with few morphological differences that can be used to differentiate wild types and hybrids properly, the problem demands a straightforward molecular approach. Here, we report a simple PCR-based technique that can differentiate the hybrid variety from wild types easily using three different microsatellite markers. Three sets of primers were used to amplify three different microsatellite markers from the genomic DNA isolated from pectoral fins. When the PCR products using all three primer sets were analyzed, 'hybrid-Rohu' could be distinguished from wild types. Whereas the hybrid-Rohu DNA yielded specific PCR products with all three primer pairs, only two PCR products were obtained either from wild-type Catla DNA (by primer sets 1 and 2) or from wild-type Rohu DNA (by primer sets 1 and 3). This study clearly demonstrates that a simple PCR-based technique will help the fish breeders and hatcheries to identify and differentiate suspected hybrid-Rohu carp from the wild types within a few hours. © 2013 Springer Science+Business Media Dordrecht. Source

Mahata M.,University of California at San Diego | Zhang K.,University of California at San Diego | Nandi S.,University of California at San Diego | Brar B.K.,University of California at San Diego | And 5 more authors.
Cell and Tissue Research | Year: 2011

Pituitary adenylyl cyclase activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP) augment the biosynthesis of tyrosine hydroxylase (TH). We tested whether secretin belonging to the glucagon/PACAP/VIP superfamily would increase transcription of the tyrosine hydroxylase (Th) gene and modulate catecholamine secretion. Secretin activated transcription of the endogenous Th gene and its transfected promoter (EC50 ∼4.6 nM) in pheochromocytoma (PC12) cells. This was abolished by pre-treatment with a secretin receptor (SCTR) antagonist and by inhibition of protein kinase A (PKA), mitogen-activated protein kinase, or CREB (cAMP response element-binding protein). In agreement, secretin increased PKA activity and induced phosphorylation of CREB and binding to Th CRE, suggesting secretin signaling to transcription via a PKA-CREB pathway. Secretin stimulated catecholamine secretion (EC50 ∼3.5 μM) from PC12 cells, but this was inhibited by pre-treatment with VIP-preferring receptor (VPAC1)/PACAP-preferring receptor (PAC1) antagonists. Secretin-evoked secretion occurred without extracellular Ca2+ and was abolished by intracellular Ca2+ chelation. Secretin augmented phospholipase C (PLC) activity and increased inositol-1,4,5-triphosphate (IP3) levels in PC12 cells; PLC-β inhibition blocked secretin-induced catecholamine secretion, indicating the participation of intracellular Ca2+ from a phospholipase pathway in secretion. Like PACAP, secretin evoked long-lasting catecholamine secretion, even after only a transient exposure. Thus, transcription is triggered by nanomolar concentrations of the peptide through SCTR, with signaling along the cAMP-PKA and extracellular-signal-regulated kinase 1/2 pathways and through CREB. By contrast, secretion is triggered only by micromolar concentrations of peptide through PAC1/VPAC receptors and by utilizing a PLC/intracellular Ca 2+ pathway. © 2011 Springer-Verlag. Source

Akhouri B.P.,Birsa College | Akhtar K.,Ranchi College
Oriental Journal of Chemistry | Year: 2016

Analysis of the equations of state of the hard convex body chain and hard spheres has been done for predicting the vapor liquid equilibrium of simple fluids of n-alkanes. The repulsive part of the Boublik equation of state for the hard convex body chain has been found as an equivalent alternative either for the well known Carnahan-Starling repulsive term or the established van der Waals repulsive part of hard spheres equations of state. The attractive parts of these equations of state have the similar form as that of the van der Waals and are obeying the power-law temperature dependency. Add-on separation method of compressibility factor has been used for these equations of state. The simulated data for VLE densities from these equations of state are found to agree well with the available experimental data for n-alkane fuids. Source

Singh N.,Ranchi College | Varshney D.,Devi Ahilya University | Kapoor A.,Ranchi University | Dey S.K.,Ranchi University
Optik | Year: 2013

The propagation characteristics of electromagnetic waves through eccentric core optical fibers have been discussed making use of scalar wave equation in conjunction with Graf's addition theorem for cylindrical Bessel's functions. The system under investigation consists of three media with eccentric location of the inner core region. This makes the system birefringent and lifts up the degeneracy present in the corresponding structure with cylindrically symmetric inner core region. Furthermore, the birefringence increases with the increase in eccentricity up to an allowed limit dictated by the geometrical parameters of the system and makes the propagation truly single mode single polarization. © 2013 Elsevier GmbH. All rights reserved. Source

Saw S.K.,Central Instrumentation Facility | Akhtar K.,Ranchi College | Yadav N.,BIT | Singh A.K.,Central Instrumentation Facility
Journal of Natural Fibers | Year: 2014

The action of water in natural fiber-reinforced composite material was studied so as to produce great swelling with resultant changes in the fine structure, dimensional stability, and mechanical properties. Water absorption and thickness swelling test reveal that hybrid composite shows water absorption moderately, which is 15.3% for hybrid coir/jute/coir composite and 11.2% for hybrid jute/coir/jute composite. The thickness swelling, water absorption, and mechanical properties of the hybrid composites slightly increased as the layering pattern of hybrid composites changed. Hybridization of coir fibers composites with jute fibers can improve the dimensional stability, extensibility and density of pure coir composites. Microstructures of the composites were examined to understand the mechanisms for the fiber-matrix interaction in relation to mechanical properties. © 2014 Copyright © Taylor & Francis Group, LLC. Source

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