Rama University

Kānpur, India

Rama University

Kānpur, India

Time filter

Source Type

Thomas D.,Manav Rachna International University | Singh G.K.,Rama University | Mishra S.,University of Sao Paulo
Materials Today: Proceedings | Year: 2015

Electric Discharge Machining (EDM) has been used for machining of electrically conductive difficult materials and Wire EDM is a kind of EDM where a wire is used as the tool and machining takes place due to erosion. Usually, Copper and brass wires were effectively used for machining in WEDM but it was found that the productivity and characteristics for coated wires had better results hence zinc coated wires will be used. The study has been conducted for the modelling of a WEDM process performed on the Tool Steel EN31. Surface Roughness (SR) is the major performance characteristic that is evaluated. The corresponding parameters acting as the input are Current, Pulse on Time and Pulse off Time, Wire Tension. The central composite design is utilized to plan the experiment and Response Surface Methodology is implied and an experimental model has been developed. Analysis on machining characteristic of coated WEDM is made based on the developed model. The model has been verified and checked for adequacy. © 2015 Elsevier Ltd.


Singh G.K.,Rama University | Thomas D.,Departmnt of Mechanical Engineering Manav Rachna University | Srivastava T.,Gautam Buddha University
Materials Today: Proceedings | Year: 2015

Wire electric discharge machining, a non-traditional machining process comprising zinc coated wire electrode, has been developed for machining of electrically conductive difficult-to-machine very hard materials. Modernization of mechanical industry has lead to increase in demand for hard and tough materials and, having wide applications in aircraft and rocket industries, is important from the quality of cut point of view. Keeping this in view, a hybrid approach of Taguchi method (TM) and principal component analysis (PCA) has been applied for multi-objective optimization (MOO) WEDM of AISI D3 tool steel to achieve better cut qualities within existing resources. The quality characteristics MRR, have been considered for simultaneous optimization. The input parameters considered are pulse on time .pulse off time, peak current and servo voltage. Initially, single-objective optimization has been performed using TM and then the signal-to-noise (S/N) ratios obtained from TM have been further used in PCA for multi-objective optimization. The results of MOO include the prediction of optimum input parameter level and their relative significance on multiple quality characteristics (MQC). © 2015 Elsevier Ltd.


PubMed | Rama University, Bhagwan Mahavir Medical Superspeciality Hospital, PDU Dental College, Index Institute of Dental science and Sharda University
Type: Journal Article | Journal: The journal of contemporary dental practice | Year: 2016

Various agents are used these days for increasing the esthetics. One such procedure is bleaching that offers various advantages, as it is minimal invasive and cheap option to color the teeth and remove stain. The altered enamel after the bleaching process shows surface demineralization and porosities. The present study aimed to evaluate the effect of different bleaching agents on the microhardness of enamel.A total of 100 freshly human extracted maxillary premolar teeth were selected for the study. Teeth with sound tooth structure were included for the study. All the specimens were randomly divided into four groups with 25 specimens in each group depending upon the type of bleaching agent used: Group A, artificial saliva (Control group); Group B, 35% hydrogen peroxide (HP); Group C, 25% HP; Group D, 10% carbamide peroxide (CP). Knoop Hardness Number (KHN) was calculated at 24, 48-hour, and 7-week interval.Results showed no statistical significant differences between the microhardness of enamel of different groups (p < 0.005). A slight fall in the value of KHN was seen in all the groups, except for the control group, although the results were statistically nonsignificant (p > 0.005).Although nonsignificantly, all the bleaching solutions produced some amount of alterations in the microstructure of enamel. More studies with higher study groups and more advanced estimation technologies are required to minimize microstructure alterations and promote for better outcome of bleaching procedures.


PubMed | Rama University and Madhav Institute of Technology and Science
Type: | Journal: Advanced biomedical research | Year: 2015

Lassa fever is a severe, often-fatal and one of the most virulent disease in primates. However, the mechanism of escape of virus from the T-cell mediated immune response of the host cell is not explained in any studies yet. In our studies we had aimed to predict B- and T- cell epitope of Lassa virus protein, for impaling the futuristic approach of developing preventive measures against this disease, further we can also study its presumed viral- host mechanism.Peptide based subunit vaccine was developed from all four protein against Lassa virus. We adopted sequence, 3D structure and fold level in silico analysis to predict B-cell and T-cell epitopes. The 3-D structure was determined for all protein by homology modeling and the modeled structure validated.One T-cell epitope from Glycoprotein (WDCIMTSYQ) and one from Nucleoprotein (WPYIASRTS) binds to maximum no of MHC class I and MHC class II alleles. They also specially bind to HLA alleles namely, A*0201, A*2705, DRB*0101 and DRB*0401.Taken together, the results indicate the Glycoprotein and nucleoprotein are most suitable vaccine candidates against Lassa virus.


Thomas D.,ManavRachna University | Kumar R.,P.A. College | Singh G.K.,Rama University | Sinha P.,P.A. College | Mishra S.,University of Sao Paulo
Materials Today: Proceedings | Year: 2015

Electric Discharge Machining (EDM) has been used for machining of electrically conductive difficult materials. Wire EDM is a kind of EDM where a wire is used to act as the tool and due to erosion, machining takes place. Generally, Brass and Copper wires were effectively used for machining but it was found that the productivity and characteristics for coated wires had better results hence coated wires (zinc) will be used. The study has been conducted for the optimization and design of WEDM process performed on the Tool Steel EN31. The major performance characteristic that is evaluated is Material Removal Rate (MRR). The corresponding parameters acting as the input are Current, Pulse on Time and Pulse off Time, Wire Tension. The central composite design is utilized to plan the experiment and Response Surface Methodology is implied and an experimental model has been developed. Analysis on machining characteristic of coated WEDM is made based on the developed model. The model has been verified and checked for adequacy. © 2015 Elsevier Ltd.


Sharma P.,IFTM University | Sharma P.,Mangalayatan University | Srivastav S.,Mangalayatan University | Mishra S.,IFTM University | Kumar A.,Rama University
International Journal of Peptide Research and Therapeutics | Year: 2016

Japanese encephalitis is a major threat in developing countries, even the availability of several conventional vaccines, which demand development of more effective vaccines. The present study used propred I and Immune Epitope Database Artificial Neural Network (ANN) algorithm (IEDB-ANN) to identify the conserve and promiscuous T cell epitopes from JEV proteome followed by structure based analysis of potential epitopes. Among all identified 102 epitopes, ten epitope were promiscuous but two epitopes of glycoprotein viz. 55LVTVNPFVA63 and 38IPIVSVASL46 were found most promiscuous, highly conserved and high population coverage in comparison of known antigenic positive control peptides. The B cell epitopes of glycoprotein also share these two T cell epitopes revealed by BCPred algorithm which can be a basis to confer the protection by neutralizing antibody combined with an effective cell-mediated response. Further, Autodock 4.2 and NAMD–VMD molecular dynamics simulation were used for docking and molecular dynamics simulation respectively, to validate epitope and allele complex binding stability. The 3D structure models were generated for epitopes and corresponding HLA allele by Pepstr and Modeller 9.10 respectively. Epitope LVTVNPFVA–B5101 allele complex showed best energy minimization and stability over the time window during simulation. Here we also present the binding sequel of epitope LVTVNPFVA and its eventual transport through cTAP1 (PDB ID: 1JJ7) revealed by Autodock 4.2, which is an essential path for HLA class I binding epitopes to elicit immune response. The docking experiment of epitope LVTVNPFVA and cTAP1 very well show a 2 H-bond with a binding energy of −1.88 kcal/mol and other binding state of epitope forming no H-bond with a binding energy of −1.13 kcal/mol in the lower area of cTAP1 cavity. These results show a smooth pass through of the epitope across the channel of cTAP1. Overall, identified peptides have potential application in the design and development of short peptide based vaccines and diagnostic agents for Japanese encephalitis. © 2016 Springer Science+Business Media New York


Sinha P.,P.A. College | Kumar R.,P.A. College | Singh G.K.,Rama University | Thomas D.,Departmnt of Mechanical Engineering Manav Rachna University
Materials Today: Proceedings | Year: 2015

Wire electric discharge machining, a non-traditional machining process comprising zinc coated wire electrode, has been developed for machining of electrically conductive difficult-to-machine very hard materials. Modernization of mechanical industry has lead to increase in demand for hard and tough materials and, having wide applications in aircraft and rocket industries, is important from the quality of cut point of view. Keeping this in view, a hybrid approach of Taguchi method (TM) and principal component analysis (PCA) has been applied for multi-objective optimization (MOO) WEDM of AISI D3 tool steel to achieve better cut qualities within existing resources. The three-quality characteristics MRR, Length of Cut, and Surface roughness have been considered for simultaneous optimization. The input parameters considered are pulse on time .pulse off time, peak current and servo voltage. Initially, single-objective optimization has been performed using TM and then the signal-to-noise (S/N) ratios obtained from TM have been further used in PCA for multi-objective optimization. The results of MOO include the prediction of optimum input parameter level and their relative significance on multiple quality characteristics (MQC). © 2015 Elsevier Ltd.


Prajapat R.,Rama University | Bhattacharya I.,Rama University | Kumar A.,Rama University
Trends in Bioinformatics | Year: 2016

Background: New study findings indicate that variation in the transcription factor 7-like 2 (TCF7L2) gene, linked to the pathogenesis of type 2 diabetes. In the present study, the protein structure model of TCF7L2 was generated, to understand the structure, function and mechanism of the action of proteins. The present study was designed to enlist some of the physiochemical and functional properties of TCF7L2 protein and provides information about its three-dimensional structure. Materials and Methods: The PDB file of TCF7L2 [CAG38811] was generated by Phyre 2 servers. Model construction and regularization (including geometry optimization) of model were done by optimization protocol in YASARA. The energy of the model was minimized using the standard protocols of combined application of simulated annealing, conjugate gradient and steepest descent. The UCLA-DOE server was used to visual analysis of the quality of a putative crystal structure for protein. The validation for structure models was performed by using PROCHECK. The model was further analyzed by WHATIF, QMEAN and ProSA. Results: The model showed good stereo-chemical property in terms of overall G-factor value of -0.64 indicating that geometry of model corresponds to the probability conformation with 67.9% residue in the core region of Ramachandran plot showing high accuracy of model prediction. The Z-score of -6.07 predicted by ProSA represents the good quality of the model. The Z-score also measures the divergence of total energy of the structure with respect to an energy distribution derived from random conformations. The scores indicate a highly reliable structure and are well within the range of scores typically found for proteins of similar size. The energy plot shows the local model quality by plotting knowledge-based energies as a function of amino acid sequence position. Conclusions: The generated model could be supportive to understand the functional characteristics of transcription factor 7-like 2 (TCF7L2). The variants in TCF7L2 associated with the risk for type 2 diabetes. © 2016 Rajneesh Prajapat et al.

Loading Rama University collaborators
Loading Rama University collaborators