Rajiv Gandhi University of Health science, centered in Bangalore, India, is a unitary university set up in 1996 by the government of Karnataka, India, for the regulation and promotion of higher education in health science throughout the state of Karnataka. Wikipedia.
Pawar H.,Institute of Bioinformatics |
Pawar H.,Rajiv Gandhi University of Health Sciences |
Kulkarni A.,National Center for Cell science |
Dixit T.,National Center for Cell science |
And 2 more authors.
Genomics | Year: 2014
Leishmania donovani is a kinetoplastid protozoan parasite which causes the fatal disease visceral leishmaniasis in humans. Genome sequencing of L. donovani revealed information about the arrangement of genes and genome architecture. After curation of the genome sequence, many genes in L. donovani were assigned as truncated or "partial" genes by the genome sequencing group. In the present study, we have carried out an extensive analysis and attempted to improve the gene models of these partial genes. Our analysis resulted in the identification of 308 partial genes in L. donovani, which were further categorized as C-terminal extensions, joining of genes, tandemly repeated paralogs and wrong chromosomal assignments. We have analyzed each of these genes from these categories and have improved the annotation of existing gene models in L. donovani. Some of these corrections have been confirmed by mass spectrometry derived peptide data from our previous comparative proteogenomics study in L. donovani. © 2014 Elsevier Inc.
Muthukrishnan R.,Rajiv Gandhi University of Health Sciences |
Muthukrishnan R.,Guru Nanak Dev University |
Shenoy S.D.,Guru Nanak Dev University |
Jaspal S.S.,Guru Nanak Dev University |
And 2 more authors.
Sports Medicine, Arthroscopy, Rehabilitation, Therapy and Technology | Year: 2010
Background: The purpose of the present study was to examine the differential effect of core stability exercise training and conventional physiotherapy regime on altered postural control parameters in patients with chronic low back pain (CLBP). As heterogeneity in CLBP population moderates the effect of intervention on outcomes, in this study, interventions approaches were used based on sub-groups of CLBP.Methods: This was an allocation concealed, blinded, sequential and pragmatic control trial. Three groups of participants were investigated during postural perturbations: 1) CLBP patients with movement impairment (n = 15, MI group) randomized to conventional physiotherapy regime 2) fifteen CLBP patients with control impairment randomized to core stability group (CI group) and 3) fifteen healthy controls (HC).Results: The MI group did not show any significant changes in postural control parameters after the intervention period however they improved significantly in disability scores and fear avoidance belief questionnaire work score (P < 0.05). The CI group showed significant improvements in Fx, Fz, and My variables (p < 0.013, p < 0.006, and p < 0.002 respectively with larger effect sizes: Hedges's g > 0.8) after 8 weeks of core stability exercises for the adjusted p values. Postural control parameters of HC group were analyzed independently with pre and post postural control parameters of CI and MI group. This revealed the significant improvements in postural control parameters in CI group compared to MI group indicating the specific adaptation to the core stability exercises in CI group. Though the disability scores were reduced significantly in CI and MI groups (p < 0.001), the post intervention scores between groups were not found significant (p < 0.288). Twenty percentage absolute risk reduction in flare-up rates during intervention was found in CI group (95% CI: 0.69-0.98).Conclusions: In this study core stability exercise group demonstrated significant improvements after intervention in ground reaction forces (Fz, Mz; g > 0.8) indicating changes in load transfer patterns during perturbation similar to HC group.Trial registration: UTRN095032158-06012009423714. © 2010 Muthukrishnan et al; licensee BioMed Central Ltd.
Babu K.S.,Andhra University |
Rao J.V.L.N.S.,Andhra University |
Bhargava K.V.,Rajiv Gandhi University of Health Sciences
Rasayan Journal of Chemistry | Year: 2011
A simple and easy to use reverse phase HPLC method for the determination of Aliskiren hemifumarate in tablet dosage forms was developed. Liquid chromatographic separation of aliskiren was achieved on a Waters Xbridge C18 (150 × 4.6mm, 5μM particle size) column employing a mobile phase of 0.03 Trifluroacetic acid (TFA) in water and 0.03%TFA in Acetonitrile and water (95:5) at a flow rate of 0.8mL/min. Aliskiren was monitored using a dual mode wavelength detector at 230/254nm. The method was linear in the concentration of 1-100 μg/mL and the limit of detection and limit of quantification of the method was 0.2μg/mL and 0.6μg/mL. The method developed had also been validated for its accuracy, robustness, specificity and repeatability and succefully applied to the determination of aliskiren in pharmaceutical dosage forms The tablets. The method offers good sensitivity and reliable accuracy to be used for routine analysis. © 2011 RASYAN. All rights reserved.
Maheswari E.,Rajiv Gandhi University of Health Sciences |
Saraswathy G.R.L.,Rajiv Gandhi University of Health Sciences |
Santhranii T.,Andhra University
Indian Journal of Pharmacology | Year: 2014
Objectives: The present study evaluates the hepatoprotective activity of N-acetyl cysteine (NAC) against carbamazepine (CBZ)-induced hepatotoxicity. Materials and Methods: Rats were treated with CBZ (50 mg/kg p.o.) and CBZ supplemented with NAC 50, 100 and 200 mg/kg for 45 days, after which blood samples were collected and subjected to liver function tests. Animals were killed, liver was separated, weighed and the levels of antioxidants and liver enzymes were estimated. In addition, histopathological investigation was also performed. Results: Serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate (SGOT) transaminase, alkaline phosphatase (ALP), bilirubin, lipid peroxidation, absolute and relative liver weights were significantly (P < 0.05) elevated, whereas serum levels of albumin, total protein and body weight were decreased in the CBZ-treated animals. CBZ also produced vacuolar degeneration, centrilobular congestion and hepatic necrosis as evidenced from histopathological report. NAC significantly reduced the levels of serum transaminase, ALP, bilirubin and liver weight and increased the levels of total protein, albumin and body weight. Conclusion: It was observed that NAC increased the glutathione (GSH) content, reduced lipid peroxidation and reversed the CBZ-induced histopathological abnormalities. CBZ-induced hepatotoxicity may be due its toxic epoxide metabolite-induced oxidative stress.
Mitra S.K.,Rajiv Gandhi University of Health Sciences |
Prakash N.S.,Himalaya |
Indian Journal of Pharmacology | Year: 2012
Objectives: The anticancer activity of shatavarins (containing shatavarin IV) isolated from the roots of Asparagus racemosus (Wild) was evaluated using in vitro and in vivo experimental models. Material and Methods: The shatavarin IV was isolated from ethyl acetate insoluble fraction (AR-2B) of chloroform:methanol (2:1) (AR-2) extract of A. racemosus roots. The cytotoxicity (in vitro) of shatavarin IV and other shatavarins rich fraction was carried out using of MTT assay using MCF-7 (human breast cancer), HT-29 (human colon adenocarcinoma), and A-498 (human kidney carcinoma) cell lines. The in vivo anticancer activity of shatavarins (containing shatavarin IV) was evaluated against Ehrlich ascites carcinoma (EAC) tumor bearing mice. Results: The isolated shatavarin IV (84.69 %) along with shatavarins rich fraction, coded AR-2B containing 5.05% shatavarin IV showed potent cytotoxicity. Oral administration of AR-2B to tumor bearing mice at doses of 250 and 500 mg/kg body weight for 10 days, showed significant reduction in percent increase in body weight, tumor volume, packed cell volume, viable tumor cell count, and increased non-viable cell count when compared to the untreated mice of the EAC control group. The restoration of hematological parameters towards normalcy was also observed. Conclusion: The result suggests that the shatavarins (containing shatavarin IV) rich fraction (AR-2B) exhibits significant anticancer activity in both in vitro and in vivo experimental models.
Rajappa G.C.,Rajiv Gandhi University of Health Sciences |
Anandaswamy T.C.,Rajiv Gandhi University of Health Sciences
Anesthesiology and Pain Medicine | Year: 2014
Introduction: Pheochromocytoma is a catecholamine-secreting tumor, which is seen rarely in children. These tumors predominantly secrete norepinephrine and epinephrine. They might be familial and associated with hereditary tumors such as Von Hippel-Lindau syndrome and multiple endocrine neoplasia type II. Case Presentation: The child might present with a spectrum of clinical manifestation including hypertension, headache, visual disturbances, and behavioral problems. A meticulous preoperative preparation is essential for a stable intraoperative and postoperative outcome Conclusions: We described successful perioperative management of a child who underwent bilateral laparoscopic cortical sparing adrenalectomy and a repeated surgery for the residual tumor removal. © 2014, Iranian Society of Regional Anesthesia and Pain Medicine (ISRAPM).
Kumar P.,Rajiv Gandhi University of Health Sciences
Oral health and dental management | Year: 2013
Oral lichen planus (OLP) is a chronic mucocutaneous disease with an unknown aetiology, affecting 0.5-2% of the population and with a predilection for females in fourth to fifth decade of life. Most oral lichen planus lesions are asymptomatic but the atrophic and erosive forms of OLP can cause symptoms ranging from spontaneous soreness to severe pain interfering with eating, speech and swallowing. Various drugs have been used for the treatment of OLP including corticosteroids and other immunomodulators. However, no therapy is considered as the single most effective and without side effects in the management of this enigmatic disease. This paper presents a case of successful management of extensive, symptomatic atrophic OLP with a novel treatment protocol: oral mini pulse therapy with betamethasone. In spite of using long-term systemic corticosteroids, side effects were minimal and clinically uneventful. Further controlled trials with this therapy may provide a definitive mode of treatment for severe OLP cases.
Xavier A.M.,Rajiv Gandhi University of Health Sciences |
Hegde A.M.,Rajiv Gandhi University of Health Sciences
Asian Pacific Journal of Cancer Prevention | Year: 2010
The leukemias are the most common form of childhood malignancy. The pediatric dental professional plays a major role in the prevention, stabilization and treatment of the oral and dental problems that can compromise the child's health and quality of life before, during and after the cancer treatment. This manuscript highlights the incidence of oral complications in leukemic children receiving oncology treatment and the systematic preventive protocol followed during different phases of medical treatment.
Munir A.R.,Rajiv Gandhi University of Health Sciences |
Prem K.D.,Rajiv Gandhi University of Health Sciences
Journal of Complementary and Integrative Medicine | Year: 2016
Background: There is a growing awareness among teachers in the complementary and alternative medicine (CAM) disciplines that a formal training in educational methodology can improve their performance as teachers and student evaluators. The Training of Trainers programs conducted by Rajiv Gandhi University of Health Sciences, Karnataka, in the previous years have brought about a transformation among the teachers who attended those programs. Also the teachers were witness to a changing perception among students towards teachers who adapt innovative teaching/assessment strategies. This report illustrates an innovative training activity that was adapted to design a reference model that can be developed as an operational model for large-scale execution. Methods: Teachers who are under the affiliated CAM Institutions in Rajiv Gandhi University of Health Sciences, Karnataka, participated in a three-month 'Short Course in Educational Methodology'. This program was delivered on distance learning mode. The course was organised into four modules. Study material was provided for each of the module in the form of a study guide and related reference articles in electronic form. There were three contact programs - Induction and Introduction that also addressed overview of entire course and the subject matter of Module 1, and this was at the beginning of the course, first contact program to address the learner needs of Modules 2 and 3 and second contact program for the contents in Module 4. The participants were engaged during the entire course duration with interactive contact programs, self-study and application of concepts in their teaching/assessment practices, submission of assignments online, and microteaching presentation and peer review. Results: The documentation and raw data generated during the course of training were used to generate an operational model for training of university teachers of health sciences faculty in general and teachers of CAM disciplines in particular. Conclusions: Establishing a model of training for university teachers who are engaged in health sciences education provides a strong platform to realise the roles of teacher, evolve as a conscientious and committed teacher and infuse their learners with passion and commitment to become competent in their professional performance. © 2016 by De Gruyter.
Prakash N.S.,Himalaya |
Sundaram R.,Himalaya |
Mitra S.K.,Rajiv Gandhi University of Health Sciences
American Journal of Pharmacology and Toxicology | Year: 2011
Problem statement: Natural products have long been a fertile source of cure for cancer, which is projected to become the major cause of death in this century. Major classes of anticancer compounds include alkaloids, terpenoids, flavonoids and lignans. We have chosen terpenoids (bacosides) because terpenoids like taxol are currently being used in cancer chemotherapy. The anticancer activity of Bacoside A (containing Bacoside A3) isolated from the whole plant of plant Bacopa monnieiri (Linn.) was evaluated in in vitro and in vivo experimental models. Approach: The Bacoside A was isolated from ethyl acetate insoluble fraction (BM-2B) of chloroform: Methanol (2: 1) (BM-2) extract of B. monnieiri whole plant. The cytotoxicity (in vitro) of Bacoside A (BM2BF 8-BSD) was carried out by means of MTT assay using MCF-7 (Human breast cancer), HT-29 (Human colon adeno carcinoma) and A-498 (Human kidney carcinoma) cell lines. The in vivo anticancer activity of Bacoside A was evaluated against Ehrlich ascites carcinoma (EAC) tumor bearing mice. Results: The Bacoside A (31.38%) rich fraction, coded BM-2B containing Bacoside A3 (8.09%) was showing potent cytotoxicity. Oral administration of BM-2B to tumor bearing mice at the dose of 250 and 500 mg kg -1 body weight for 10 days, showed significant reduction in percent increase in body weight, tumor volume, packed cell volume, viable tumor cell count and increased non-viable cell count when compared to the untreated mice of the EAC control group. The restoration of hematological parameters towards normalcy was also observed. Conclusion: The results suggests that the Bacoside A (31.38% Bacoside A containing 8.09% Bacoside A3) rich fraction (BM-2B) exhibits significant anticancer activity in both in vitro and in vivo experimental models.