Rajiv Gandhi University of Health science, centered in Bangalore, India, is a unitary university set up in 1996 by the government of Karnataka, India, for the regulation and promotion of higher education in health science throughout the state of Karnataka. Wikipedia.
Babu K.S.,Andhra University |
Rao J.V.L.N.S.,Andhra University |
Bhargava K.V.,Rajiv Gandhi University of Health Sciences
Rasayan Journal of Chemistry | Year: 2011
A simple and easy to use reverse phase HPLC method for the determination of Aliskiren hemifumarate in tablet dosage forms was developed. Liquid chromatographic separation of aliskiren was achieved on a Waters Xbridge C18 (150 × 4.6mm, 5μM particle size) column employing a mobile phase of 0.03 Trifluroacetic acid (TFA) in water and 0.03%TFA in Acetonitrile and water (95:5) at a flow rate of 0.8mL/min. Aliskiren was monitored using a dual mode wavelength detector at 230/254nm. The method was linear in the concentration of 1-100 μg/mL and the limit of detection and limit of quantification of the method was 0.2μg/mL and 0.6μg/mL. The method developed had also been validated for its accuracy, robustness, specificity and repeatability and succefully applied to the determination of aliskiren in pharmaceutical dosage forms The tablets. The method offers good sensitivity and reliable accuracy to be used for routine analysis. © 2011 RASYAN. All rights reserved.
Mitra S.K.,Rajiv Gandhi University of Health Sciences |
Prakash N.S.,Himalaya |
Indian Journal of Pharmacology | Year: 2012
Objectives: The anticancer activity of shatavarins (containing shatavarin IV) isolated from the roots of Asparagus racemosus (Wild) was evaluated using in vitro and in vivo experimental models. Material and Methods: The shatavarin IV was isolated from ethyl acetate insoluble fraction (AR-2B) of chloroform:methanol (2:1) (AR-2) extract of A. racemosus roots. The cytotoxicity (in vitro) of shatavarin IV and other shatavarins rich fraction was carried out using of MTT assay using MCF-7 (human breast cancer), HT-29 (human colon adenocarcinoma), and A-498 (human kidney carcinoma) cell lines. The in vivo anticancer activity of shatavarins (containing shatavarin IV) was evaluated against Ehrlich ascites carcinoma (EAC) tumor bearing mice. Results: The isolated shatavarin IV (84.69 %) along with shatavarins rich fraction, coded AR-2B containing 5.05% shatavarin IV showed potent cytotoxicity. Oral administration of AR-2B to tumor bearing mice at doses of 250 and 500 mg/kg body weight for 10 days, showed significant reduction in percent increase in body weight, tumor volume, packed cell volume, viable tumor cell count, and increased non-viable cell count when compared to the untreated mice of the EAC control group. The restoration of hematological parameters towards normalcy was also observed. Conclusion: The result suggests that the shatavarins (containing shatavarin IV) rich fraction (AR-2B) exhibits significant anticancer activity in both in vitro and in vivo experimental models.
Kumar P.,Rajiv Gandhi University of Health Sciences
Oral health and dental management | Year: 2013
Oral lichen planus (OLP) is a chronic mucocutaneous disease with an unknown aetiology, affecting 0.5-2% of the population and with a predilection for females in fourth to fifth decade of life. Most oral lichen planus lesions are asymptomatic but the atrophic and erosive forms of OLP can cause symptoms ranging from spontaneous soreness to severe pain interfering with eating, speech and swallowing. Various drugs have been used for the treatment of OLP including corticosteroids and other immunomodulators. However, no therapy is considered as the single most effective and without side effects in the management of this enigmatic disease. This paper presents a case of successful management of extensive, symptomatic atrophic OLP with a novel treatment protocol: oral mini pulse therapy with betamethasone. In spite of using long-term systemic corticosteroids, side effects were minimal and clinically uneventful. Further controlled trials with this therapy may provide a definitive mode of treatment for severe OLP cases.
Pawar H.,Institute of Bioinformatics |
Pawar H.,Rajiv Gandhi University of Health Sciences |
Kulkarni A.,National Center for Cell Science |
Dixit T.,National Center for Cell Science |
And 2 more authors.
Genomics | Year: 2014
Leishmania donovani is a kinetoplastid protozoan parasite which causes the fatal disease visceral leishmaniasis in humans. Genome sequencing of L. donovani revealed information about the arrangement of genes and genome architecture. After curation of the genome sequence, many genes in L. donovani were assigned as truncated or "partial" genes by the genome sequencing group. In the present study, we have carried out an extensive analysis and attempted to improve the gene models of these partial genes. Our analysis resulted in the identification of 308 partial genes in L. donovani, which were further categorized as C-terminal extensions, joining of genes, tandemly repeated paralogs and wrong chromosomal assignments. We have analyzed each of these genes from these categories and have improved the annotation of existing gene models in L. donovani. Some of these corrections have been confirmed by mass spectrometry derived peptide data from our previous comparative proteogenomics study in L. donovani. © 2014 Elsevier Inc.
Prakash N.S.,Himalaya |
Sundaram R.,Himalaya |
Mitra S.K.,Rajiv Gandhi University of Health Sciences
American Journal of Pharmacology and Toxicology | Year: 2011
Problem statement: Natural products have long been a fertile source of cure for cancer, which is projected to become the major cause of death in this century. Major classes of anticancer compounds include alkaloids, terpenoids, flavonoids and lignans. We have chosen terpenoids (bacosides) because terpenoids like taxol are currently being used in cancer chemotherapy. The anticancer activity of Bacoside A (containing Bacoside A3) isolated from the whole plant of plant Bacopa monnieiri (Linn.) was evaluated in in vitro and in vivo experimental models. Approach: The Bacoside A was isolated from ethyl acetate insoluble fraction (BM-2B) of chloroform: Methanol (2: 1) (BM-2) extract of B. monnieiri whole plant. The cytotoxicity (in vitro) of Bacoside A (BM2BF 8-BSD) was carried out by means of MTT assay using MCF-7 (Human breast cancer), HT-29 (Human colon adeno carcinoma) and A-498 (Human kidney carcinoma) cell lines. The in vivo anticancer activity of Bacoside A was evaluated against Ehrlich ascites carcinoma (EAC) tumor bearing mice. Results: The Bacoside A (31.38%) rich fraction, coded BM-2B containing Bacoside A3 (8.09%) was showing potent cytotoxicity. Oral administration of BM-2B to tumor bearing mice at the dose of 250 and 500 mg kg -1 body weight for 10 days, showed significant reduction in percent increase in body weight, tumor volume, packed cell volume, viable tumor cell count and increased non-viable cell count when compared to the untreated mice of the EAC control group. The restoration of hematological parameters towards normalcy was also observed. Conclusion: The results suggests that the Bacoside A (31.38% Bacoside A containing 8.09% Bacoside A3) rich fraction (BM-2B) exhibits significant anticancer activity in both in vitro and in vivo experimental models.