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Reddi S.,National Dairy Research Institute | Shanmugam V.P.,Rajiv Gandhi Institute of Veterinary Education and Research | Kapila S.,National Dairy Research Institute | Kapila R.,National Dairy Research Institute
European Food Research and Technology | Year: 2016

Osteoblast cells are the building blocks of bone architecture and most suitable cells for screening osteoanabolic agents. As bone formation begins with osteoblast proliferation, it is the essential stage for screening osteoanbolics. LC–MS/MS analysis of the 11th RP–HPLC fraction of pepsin–trypsin (PT) hydrolysates of buffalo casein showed 15 peptides. Out of these 15 peptides, four peptides of less than 1 kDa were custom-synthesized and evaluated for osteoblast proliferating ability. Proliferation effect of the peptides was studied by formazan crystal formation (MTT method) and relative expression of proliferation marker genes (clay and cdk2). PT hydrolysates and all the four peptides significantly induced osteoblast proliferation indicated by both MTT and relative gene expression studies. This study showed the osteoblast-promoting property of peptides derived from milk proteins, thereby further substantiating the earlier epidemiological data regarding bone health-promoting effect of milk. © 2016 Springer-Verlag Berlin Heidelberg Source


Kemgang T.S.,National Dairy Research Institute | Kemgang T.S.,University of Ngaoundere | Kapila S.,National Dairy Research Institute | Shanmugam V.P.,National Dairy Research Institute | And 2 more authors.
Journal of Applied Microbiology | Year: 2014

The mechanism by which probiotic lactobacilli affect the immune system is strain specific. As the immune system is a multicompartmental system, each strain has its way to interact with it and induce a visible and quantifiable effect. This review summarizes the interplay existing between the host immune system and probiotic lactobacilli, that is, with emphasis on lactobacilli as a prototype probiotic genus. Several aspects including the bacterial-host cross-talk with the mucosal and systemic immune system are presented, as well as short sections on the competing effect towards pathogenic bacteria and their uses as delivery vehicle for antigens. © 2014 The Society for Applied Microbiology. Source


Udgaonkar U.S.,Bharati Vidyapeeth Deemed University | Patil S.S.,Bharati Vidyapeeth Deemed University | Rekha V.B.,Rajiv Gandhi Institute of Veterinary Education and Research | Shah S.,Bharati Vidyapeeth Deemed University
Indian Journal of Medical Microbiology | Year: 2015

Diagnosis of Bacille calmette-guerine (BCG) adenitis is clinical. Conventional laboratory tests do not differentiate BCG adenitis from tuberculous adenitis. We report a case of a 3-month-old healthy baby presenting with suppurative BCG adenitis. FNAC revealed AFB on ZN-Staining, later confirmed to be Mycobacterium bovis by multiplex PCR. The treatment of suppurative BCG adenitis is needle aspiration. Anti-tubercular treatment is unwarranted. Source


Shanmugam V.P.,National Dairy Research Institute | Shanmugam V.P.,Rajiv Gandhi Institute of Veterinary Education and Research | Kapila S.,National Dairy Research Institute | Sonfack T.K.,National Dairy Research Institute | Kapila R.,National Dairy Research Institute
International Dairy Journal | Year: 2015

Bioactive peptides have been defined as specific protein fragments that have a positive impact on body functions or conditions and may ultimately influence health. We scrutinised the antioxidative property of peptides from buffalo casein digested with pepsin, trypsin, chymotrypsin alone and in combinations. Casein hydrolysates were subjected to ultrafiltration followed by reversed phase-high performance liquid chromatography (RP-HPLC). The antioxidant properties of casein, its hydrolysates and synthetic peptides, was assessed using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid). The fraction with highest Trolox equivalent antioxidant capacity (TEAC) was selected for further study. TEAC of casein was 0.862±0.11μmolmg-1, while it is highest for pepsin-trypsin hydrolysates. The 1kDa permeate of pepsin-trypsin hydrolysates possessed the highest antioxidant activity and it was resolved into 16 fractions by RP-HPLC. Antioxidant activity fraction 11 was highest and on custom sequencing yielded 4 peptides, of which the synthetic peptide VLPVPQK possessed the highest TEAC (5.71±0.59μmolmg-1). © 2014 Elsevier Ltd. Source


Ganesan R.,Pondicherry UniversityPuducherry | Ganesan R.,Rajiv Gandhi Institute of Veterinary Education and Research | Dhanavanthan P.,Pondicherry UniversityPuducherry | Balasubramanyam D.,Pondicherry UniversityPuducherry | And 3 more authors.
Indian Journal of Animal Research | Year: 2015

Sheep rearing is an important livelihood for a large number of small and marginal farmers in India. Growth is a trait of interest in domestic animals. Information about growth model parameters is very useful for selection studies. In this paper, the growth of Madras Red sheep, a native breed of Tamil Nadu state in India, is studied through nonlinear growth models using data on average monthly body weight measurements of 1424 sheep from birth to 2 years of age born during 1996 to 2010. Sex of lamb, dam weight at lambing and year of birth were the significant non-genetic factors affecting body weight at birth, 3rd, 6th, 9th and 12th month. The birth weight and 9th month weight showed significant difference between the seasons. Average daily weight gain showed significant difference between sex of lamb, season of birth and year of birth while it showed significant difference between dam weight at lambing only for the period 3-6 months. Brody’s growth model described the average monthly growth remarkably well in males and females suggesting its usefulness in growth monitoring of Madras Red sheep. © 2014, Indian Journal of Animal Research. All Rights Reserved. Source

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