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Jadhav R.N.,Rajarshi Shahu Mahavidyalaya
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2013

Actinomycetes are ubiquitous group of bacteria that occur in multiplicity of natural and man made environments. They are predominantly Gram positive bacteria with high G+C % (57-79%). Several species of actinomycetes produce antibiotics of agricultural and industrial importance. Biocontrol of plant pathogen is an attractive alternative to chemical pesticides which causes environmental pollution and development of resistant strain. Majority of antibiotics so far isolated were produced from Streptomycetes, which are common inhabitants of the soil. There are 23,000 known secondary metabolite, 42% of which are produced by actinomycetes, 42% by fungi and 16% by other bacteria. In the present study screening of actinomycetes from rhizosphere of sugarcane was done by using specific media. Plates were incubated at 28°C for 4-14 days. Ten isolates of actinomycetes were isolated. They were further purified and identified by using several biochemical tests. The antimicrobial activity of isolates of actinomycetes was studied by using agar diffusion (well) method against Bacillus megaterium NCIM2326; Candida albicans NCIM3103; Escherichia coll NCIM2064; Fusarium oxysporium (soil isolate) and Xanthomonas compestris NCIM2956. It was observed that isolates of actinomycetes ISS06 showed maximum antimicrobial activity against all test organisms. The morphology along with cultural and biochemical tests confirmed that these isolates belonged to genus Streptomyces. © Global Science Publications.


Yadav A.A.,Thin Film Physics Laboratory | Salunke S.D.,Rajarshi Shahu Mahavidyalaya
Journal of Materials Science: Materials in Electronics | Year: 2015

Metal chalcogenide and chalcopyrite thin films have attracted great deal of attention due to their exciting photoelectrical characteristics. Indium selenide thin films have been deposited by computerized chemical spray pyrolysis technique on amorphous glass substrates. The as deposited films are characterized for wide range of properties including structural, surface morphological, optical and electrical, Hall effect and thermo-electrical measurements. X-ray diffraction study revealed that indium selenide thin films are polycrystalline with hexagonal crystal structure irrespective of substrate temperature. Surface morphology and film composition have been analyzed using atomic force microscopy and energy dispersive analysis by X-rays. Nearly stoichiometric deposition of the film at 350 °C was confirmed from EDAX analysis. Optical absorption measurements show that the deposited films possess a direct band gap value of 1.78 eV. The Hall effect study reveals that the films exhibit n-type conductivity. © 2015, Springer Science+Business Media New York.


Kadam R.H.,Materials Science Research Laboratory | Biradar A.R.,Rajarshi Shahu Mahavidyalaya | Mane M.L.,Dr. Babasaheb Ambedkar Marathwada University | Shirsath S.E.,Vivekanand College | Shirsath S.E.,Shinshu University
Journal of Applied Physics | Year: 2012

Ferrite samples of Li + substituted MnFe 2O 4 nano particles were synthesized by sol-gel auto-combustion method. The samples were obtained by annealing at relatively low temperature at 600 °C and characterized by x-ray diffraction, transmission electron microscopy, vibrating sample magnetometry, and infrared (IR) spectroscopy. Lattice parameter, x-ray density, specific surface area, and porosity are found to increase, whereas bulk density and crystallite size showed the decreasing trend with the Li + content. Splitting of major absorption bands related to Li + substituted ferrites were observed in IR spectra. Substitution of Li + ions for Fe 3 caused a decrease in the saturation magnetization from 75.69 to 58.57 emu/g and the coercivity increased from 157 to 308 Oe. DC resistivity decreases with increase in Li + content. The temperature dependent results indicate that the values of dielectric constant (ε′) and loss tangent (ε″) increase with the increase in temperature. © 2012 American Institute of Physics.


Kadam R.H.,Materials Science Research Laboratory | Alone S.T.,Science and Commerce College | Mane M.L.,Rajarshi Shahu Mahavidyalaya | Biradar A.R.,Materials Science Research Laboratory | Shirsath S.E.,Shinshu University
Journal of Magnetism and Magnetic Materials | Year: 2014

Li+ substituted CoFe2O4 with the chemical formula Li3xCoFe2-xO4 were synthesized by sol-gel auto combustion method. The synthesized samples were annealed at 600°C for 4 h. X-ray diffraction data were used to evaluate the structure of the prepared samples. Spinel ferrite phase of CoFe2O4 changes to ordered like lithium ferrite phase with increase in Li+ substitution. Lattice constant increases whereas particle size found to decrease with Li+ substitution. Infrared spectroscopy also confirmed the phase transition of CoFe2O4 after the incorporation of lithium ions. Substitution of Li+ ions for Fe3+ caused a decrease in the saturation magnetization from 69.59 emu/g to 47.71 emu/g and the coercivity increased from 647 Oe to 802 Oe. Resistivity and dielectric properties shows inverse relation to each other. © 2013 Elsevier B.V. All rights reserved.


Yadav A.A.,Thin Film Physics Laboratory | Salunke S.D.,Rajarshi Shahu Mahavidyalaya
Journal of Alloys and Compounds | Year: 2015

In2Se3 thin films have been deposited onto fluorine doped tin oxide coated (FTO) glass substrates at various substrate temperatures by spray pyrolysis. The photoelectrochemical cell configurations were In2Se3 thin film/1 M (NaOH + Na2S + S)/C. From capacitance-voltage (C-V) and current-voltage (I-V) characteristics; it is concluded that In2Se3 thin films are of n-type. The Fill factor (FF) and solar conversion efficiency (η) were calculated from photovoltaic power output characteristics. In this instance, the highest measured photocurrent density of 1.05 mA/cm2 and open circuit voltage of 261 mV is observed for film deposited at 350 °C resulting in maximum power conversion efficiency (η) and fill factor (FF) to be 0.71% and 0.51% respectively. Electrochemical impedance spectroscopy study shows that the In2Se3 film deposited at 350 °C shows better performance in photoelectrochemical cell. The performance of indium selenide thin film observed in our work can motivate further studies concerning solar energy conversion. © 2015 Elsevier B.V. All rights reserved.


Sawale S.D.,G.H. Raisoni College of Engineering | Ambhore P.D.,Rajarshi Shahu Mahavidyalaya | Pawar P.P.,Sfs College | Pathak U.,Defense Research and Development Establishment | And 2 more authors.
Toxicology Mechanisms and Methods | Year: 2013

Sulfur mustard (SM; 2,2′-dichloro diethyl sulfide), an alkylating chemical warfare agent, poses a major threat in both military conflict and chemical terrorism situations. 2-chloroethyl ethyl sulfide (CEES) is a monofunctional analogue of SM, frequently used in laboratory settings, therefore increasing chances of its exposure. S-2(ω-aminoalkylamino) alkylaryl sulfide (DRDE-07) is an analogue of amifostine reported to have protective effects against SM but its effect on CEES is largely unexplored. Therefore, this study was planned to explore the effects of DRDE-07 against CEES-induced toxicity. 0.75 LD50 (1068 mg/kg) of CEES was exposed percutaneously in the presence or absence of DRDE-07 (249 mg/kg p.o.) which is given prophylactically (before 30 minute) to male mice. Animals were sacrificed on 24 h, 7th day and 14th day of CEES exposure, and tissues were collected to study oxidative stress and inflammatory markers. CEES exposure depleted intracellular GSH level and activities of GSH-linked enzymes (GR, GPx and GST) which play a major role in GSH metabolism. CEES exposure augmented lipid peroxidation indicating severe oxidative stress. It also initiated inflammation causing an increase in proinflammatory (IL1-α, IL1-β, IL-6, TNF-α and IFN-Υ) and corresponding decrease in anti-inflammatory cytokines (IL-4 and IL-10). This was also accompanied by neutrophils infiltration indicated by higher than normal myeloperoxidase (MPO) levels. DRDE-07 efficiently reduced the oxidative stress and also facilitated to resolve inflammatory alterations. This study thus evaluated the beneficial role of DRDE-07 in ameliorating the deleterious effects of CEES and can be potentially used against SM/CEES poisoning. © 2013 Informa Healthcare USA, Inc.

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