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Aramli M.S.,Tarbiat Modares University | Kalbassi M.R.,Tarbiat Modares University | Nazari R.M.,Rajaee Sturgeon Hatchery Center
Italian Journal of Zoology

An important aspect in the management and protection of endangered species from extinction is the determination of normal physiological values, including the blood and seminal plasma properties. This study was undertaken to establish a reference range for six important blood and seminal plasma enzymes. Samples of 20 Acipenser persicus Borodin, 1897 (10 female and 10 male) were analyzed. The reference ranges for the total samples and also for each sex were determined. The activities of measured enzymes in blood and semen were: alkaline phosphatase (ALP, 363.2 ± 25.24 and 12.23 ± 3.66 IU/L), acid phosphatase (ACP, 4.98 ± 0.39 and 1.86 ± 0.71 IU/L), lactate dehydrogenase (LDH, 1978.8 ± 176.5 and 472 ± 138.7 IU/L), creatine kinase (CK, 1149.6 ± 110 and 553.8 ± 119.9 IU/L), aspartate aminotransferase (AST, 51.12 ± 3.5 and 2.29 ± 1.34 IU/L) and alanine aminotransferase (ALT, 14.7 ± 0.89 and 6.9 ± 2.5 IU/L), respectively. There were significant differences between male and female fish in terms of LDH, CK and ALT activity (P < 0.05). To detect relationships among investigated parameters we used the Pearson correlation coefficient; no significant correlations were observed between these parameters in either blood or seminal plasma. © 2013 Unione Zoologica Italiana. Source

Aramli M.S.,Tarbiat Modares University | Kalbassi M.R.,Tarbiat Modares University | Nazari R.M.,Rajaee Sturgeon Hatchery Center
Comparative Clinical Pathology

The investigation of blood and seminal plasma biochemical parameters is essential for fish stock conservation and development of artificial propagation methods via extender improvement. In this research, comparison of blood and seminal plasma compounds and their relationship in Persian sturgeon, Acipenser persicus, were studied. Seminal plasma contained 59.53 ± 2.56 mEq/l sodium (Na+), 4.72 ± 0.31 mEq/l potassium (K+), 1.45 ± 0.075 mEq/l chloride (Cl−), 9.1 ± 1.42 mEq/l calcium (Ca2+), 0.7 ± 0.072 mEq/l magnesium (Mg2+), 0.11 ± 0.021 g/dl total protein, 6.67 ± 1.04 mg/dl cholesterol, 15.2 ± 0.65 mg/dl triacylglycerol, and 18.22 ± 4.16 mg/dl glucose. Also, blood biochemical values were determined (mean ± SEM) for Na+ (123.2 ± 1.31 mEq/l), K+ (2.77 ± 0.088 mEq/l), Cl− (97.18 ± 1.23 mEq/l), Ca2+ (6.67 ± 0.24 mEq/l), Mg2+ (1.29 ± 0.074 mEq/l), glucose (198.49 ± 18.03 mg/dl), triacylglycerol (214.22 ± 13.38 mg/dl), total protein (3.62 ± 0.23 g/dl), and cholesterol (127.11 ± 6.94 mg/dl). The mean blood and seminal osmolality values were 244.62 ± 3.66 and 86.9 ± 4.22 mOsm/kg, respectively. Results of the comparison between biochemical parameters of seminal and blood plasma indicated that the concentrations of all parameters of blood plasma were significantly (P < 0.05) higher than seminal plasma except potassium. The statistical Pearson correlation revealed a significant relationship between blood osmolality with levels of Mg++ and Na+ of the seminal plasma (P < 0.05), and glucose levels of blood and seminal plasma (P < 0.01). © 2013, Springer-Verlag London. Source

Aramli M.,Tarbiat Modares University | Kalbassi M.,Tarbiat Modares University | Nazari R.,Rajaee Sturgeon Hatchery Center
Reproduction in Domestic Animals

Contents: The objectives of the present study were to determine ionic and organic composition of seminal plasma, sperm concentration and their relationships in the Persian sturgeon (Acipenser persicus). In this regard, ionic content (Na+, K+, Cl-, Ca2+ and Mg2+) and organic content (total protein, glucose, cholesterol and triglyceride) along with sperm concentration were measured in 17 specimens of the Persian sturgeon. The seminal plasma contained 59.53 ± 2.56 mm/l sodium, 9.1 ± 1.42 mm chloride, 4.72 ± 0.3 mm potassium, 1.45 ± 0.075 mm calcium and 0.7 ± 0.072 mm magnesium. The following organic contents were found: total protein 0.11 ± 0.02 g/dl, glucose 22.18 ± 4.16 mg/dl, cholesterol 6.67 ± 1.04 mg/dl and triglyceride 15.2 ± 0.65 mg/dl. The mean sperm concentration was estimated to be 1.6 ± 0.12 (×109 sperm/ml). A significant relationship was found between sperm concentration and K+ of seminal plasma (r = 0.533, p < 0.05). Significant correlations were observed between ionic contents: Na+ vs Cl- (r = -0.854, p < 0.01) and Mg2+ vs K+ (-0.583, p < 0.05). Also, level of triglyceride was negatively correlated with Mg2+ (r = -0.503, p < 0.05). Presented data could be considered as a complementary study for developing special extenders and protectant solutions for improving artificial fertilization in this valuable species. © 2013 Blackwell Verlag GmbH. Source

Aramli M.S.,Urmia University | Golshahi K.,Islamic Azad University at Tehran | Nazari R.M.,Rajaee Sturgeon Hatchery Center | Aramli S.,Ardabil University of Medical Sciences

Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10°C, -15°C, -20°C, -30°C and -40°C/min) on gamete quality characteristics (i.e., duration of motility (s), motility percentage (%), ATP content (nmol/108 cells), fertilization rate (%), and hatching rate (%)) in beluga sturgeon, Huso huso. After sampling, beluga sturgeon sperm were diluted in an extender composed of 23.4mM sucrose, 0.25mM KCl, and 30mM Tris-HCl, pH 8.0 containing 10% methanol and subsequently frozen in a programmable freezer. Sperm frozen at -40°C/min resulted in means for duration of motility (134s), motility percentage (69%), ATP concentration (4.8nmol/108 cells), fertilization rate (72%) and hatching rate (65%) that were higher (P<0.05) than those for slower cooling rates. Based on our results, -40°C/min was the best freezing rate (among those tested) for cryopreservation of beluga sturgeon sperm. © 2015 Elsevier Inc.. Source

Hosseini S.V.,Tarbiat Modares University | Abedian-Kenari A.,Tarbiat Modares University | Rezaei M.,Tarbiat Modares University | Nazari R.M.,Rajaee Sturgeon Hatchery Center | And 2 more authors.
Food Chemistry

This study was conducted to investigate the effect of dietary alpha-tocopheryl acetate (vitamin E) and oil sources on fish flesh quality characteristics of Huso huso during frozen storage. Practical-type diets containing 0 or 250 mg vitamin E kg-1 with three lipid sources, fish oil (FO), soybean oil (SO) and canola oil (CO), were fed to H. huso for 120 days. Fillet samples were analysed fresh or after storage at -18 ± 1 °C for 12 months. Replacement of FO by SO and CO in diets for H. huso significantly altered the fatty acid (FA) profile, which also influenced the FA composition during frozen storage. Dietary vitamin E had a significant effect on muscle vitamin E content and lipid oxidation during storage (P > 0.05). Oxidation was reduced for fish fed vitamin E and results showed that dietary vitamin E supplementation can slow down the level of lipid oxidation in H. huso muscles during frozen storage. © 2009. Source

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