Rajaee Sturgeon Hatchery Center

Sari, Iran

Rajaee Sturgeon Hatchery Center

Sari, Iran
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Aramli M.S.,Islamic Azad University at Ardabil | Habibi E.,Tarbiat Modares University | Aramli S.,Ardebil University of Medical science | Nouri H.A.,Rajaee Sturgeon Hatchery Center
Journal of Animal Physiology and Animal Nutrition | Year: 2015

Blood and seminal plasma ionic parameters are essential for monitoring health status, detecting illnesses, fish stock conservation and development of artificial propagation methods via extender improvement. In this study, comparison of blood and seminal plasma ionic parameters in beluga, Huso huso (30-45 kg, 1-2 m, n = 10), was made. The results obtained show that Na+ (82.54 ± 5.46), Cl- (15.95 ± 0.72) and K+ (3.57 ± 0.15) were predominant ions in the seminal plasma (as mm). Blood ionic values (as mm) were determined for Na+ (110.2 ± 1.26), K+ (3.77 ± 0.081), Cl- (60.12 ± 1.5), Ca2+ (2.05 ± 0.35) and Mg2+ (1.9 ± 0.16). Results of the comparison between ionic parameters of seminal and blood plasma indicated that the concentrations of all parameters of blood plasma with the exception of K+ were significantly (p < 0.05) higher than those of seminal plasma. © 2014 Blackwell Verlag GmbH.


PubMed | Ardebil University of Medical science, Urmia University and Rajaee Sturgeon Hatchery Center
Type: Journal Article | Journal: Reproduction in domestic animals = Zuchthygiene | Year: 2016

In this study, motility and oxidative-antioxidant capacity (thiobarbituric acid-reactive substance level [TBARS], superoxide dismutase [SOD], catalase [CAT] and glutathione reductase [GR]) of beluga sturgeon (Huso huso) sperm, stored for 6days at -80C, were evaluated. After 2days of storage, sperm motility was significantly decreased (no motile sperm were observed after 6days of storage; p<.05), while TBARS and SOD values were significantly increased (p<.05). CAT and GR activities did not show significant changes among storage times (p>.05). Furthermore, all investigated parameters showed a significant difference between semen stored at 4C (control) and -80C during in vitro storage (p<.05). Data from this work can potentially be useful in sturgeon sperm cryobanking.


Aramli M.S.,Urmia University | Golshahi K.,Islamic Azad University at Tehran | Nazari R.M.,Rajaee Sturgeon Hatchery Center | Aramli S.,Ardabil University of Medical Sciences
Cryobiology | Year: 2015

Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10°C, -15°C, -20°C, -30°C and -40°C/min) on gamete quality characteristics (i.e., duration of motility (s), motility percentage (%), ATP content (nmol/108 cells), fertilization rate (%), and hatching rate (%)) in beluga sturgeon, Huso huso. After sampling, beluga sturgeon sperm were diluted in an extender composed of 23.4mM sucrose, 0.25mM KCl, and 30mM Tris-HCl, pH 8.0 containing 10% methanol and subsequently frozen in a programmable freezer. Sperm frozen at -40°C/min resulted in means for duration of motility (134s), motility percentage (69%), ATP concentration (4.8nmol/108 cells), fertilization rate (72%) and hatching rate (65%) that were higher (P<0.05) than those for slower cooling rates. Based on our results, -40°C/min was the best freezing rate (among those tested) for cryopreservation of beluga sturgeon sperm. © 2015 Elsevier Inc..


Aramli M.S.,Islamic Azad University at Ardabil | Golshahi K.,Islamic Azad University at Tehran | Nazari R.M.,Rajaee Sturgeon Hatchery Center
Aquaculture International | Year: 2014

Reuse of sturgeon broodfish for propagation could provide an opportunity to ease fishing pressure on the wild stocks of these valued species. A minimally invasive surgical technique (MIST) for the removal of ovulated eggs from the beluga, Huso huso, was tested on broodfish caught from the wild to determine whether it affected fecundity or hatching rates when compared with the traditional stripping method that involves killing the fish and removing the eggs. In comparison with the traditional procedures, MIST is rapid: it can be performed in between 8 and 15 min per fish. Morphological parameters of female beluga, germinal vesicle position, weight of obtained eggs, fertilization rate, and percentage hatching during incubation were not significantly different between the MIST and the traditional procedure. The fish remained healthy 4 weeks after surgery. The final results of this study showed that the MIST approach is efficient, practical, and less stressful to beluga broodfish during artificial propagation than other reported egg collection procedures. © 2013 Springer Science+Business Media Dordrecht.


Hosseini S.V.,Tarbiat Modares University | Abedian-Kenari A.,Tarbiat Modares University | Rezaei M.,Tarbiat Modares University | Nazari R.M.,Rajaee Sturgeon Hatchery Center | And 2 more authors.
Food Chemistry | Year: 2010

This study was conducted to investigate the effect of dietary alpha-tocopheryl acetate (vitamin E) and oil sources on fish flesh quality characteristics of Huso huso during frozen storage. Practical-type diets containing 0 or 250 mg vitamin E kg-1 with three lipid sources, fish oil (FO), soybean oil (SO) and canola oil (CO), were fed to H. huso for 120 days. Fillet samples were analysed fresh or after storage at -18 ± 1 °C for 12 months. Replacement of FO by SO and CO in diets for H. huso significantly altered the fatty acid (FA) profile, which also influenced the FA composition during frozen storage. Dietary vitamin E had a significant effect on muscle vitamin E content and lipid oxidation during storage (P > 0.05). Oxidation was reduced for fish fed vitamin E and results showed that dietary vitamin E supplementation can slow down the level of lipid oxidation in H. huso muscles during frozen storage. © 2009.


PubMed | Ardabil University of Medical Sciences, Urmia University, Rajaee Sturgeon Hatchery Center and Islamic Azad University at Tehran
Type: Journal Article | Journal: Cryobiology | Year: 2015

Broodstock selection programs are currently underway for sturgeon species. To complement and further these selection programs we need to develop sperm cryopreservation procedures. In the present study, we describe the effects of freezing rate (-10C, -15C, -20C, -30C and -40C/min) on gamete quality characteristics (i.e., duration of motility (s), motility percentage (%), ATP content (nmol/10(8) cells), fertilization rate (%), and hatching rate (%)) in beluga sturgeon, Huso huso. After sampling, beluga sturgeon sperm were diluted in an extender composed of 23.4mM sucrose, 0.25 mM KCl, and 30 mM Tris-HCl, pH 8.0 containing 10% methanol and subsequently frozen in a programmable freezer. Sperm frozen at -40C/min resulted in means for duration of motility (134 s), motility percentage (69%), ATP concentration (4.8 nmol/10(8) cells), fertilization rate (72%) and hatching rate (65%) that were higher (P<0.05) than those for slower cooling rates. Based on our results, -40C/min was the best freezing rate (among those tested) for cryopreservation of beluga sturgeon sperm.


Hosseini S.V.,Tarbiat Modares University | Hosseini S.V.,University of Tehran | Kenari A.A.,Tarbiat Modares University | Regenstein J.M.,Cornell University | And 5 more authors.
Journal of the World Aquaculture Society | Year: 2010

The main aim of this investigation was to determine the impact of a total dietary fish oil (FO) replacement by vegetable oils (soybean [SO] and canola [CO] oil) on the growth and fatty acid (FA) composition of juvenile Beluga sturgeon, Huso huso. Three practical-type diets with equal protein and lipid content were formulated using FO, SO, and CO. Each of the diets was fed to apparent satiation five times daily to H. huso (initial weight 206 ± 7.3 g) for 120 d. All groups grew equally well. Fish weight gain, condition factor, daily growth, feed intake, feed conversion, feed efficiency, protein efficiency, and survival were not affected by diet treatment. Fish lipid composition reflected the inclusion of vegetable oils and their respective FA compositions. Monounsaturated FA and polyunsaturated FA significantly increased in fish fed the CO and SO diets, respectively, but the ratio n - 3/n - 6 were significantly reduced by the inclusion of dietary vegetable oils (P < 0.05). This study suggests that FO can be replaced by SO and CO in H. huso diets under our test conditions with no significant effect on growth. However, longer assessments of these substitutions are warranted to ensure that these treatments do not have an adverse effect on fish health. © Copyright by the World Aquaculture Society 2010.


Aramli M.S.,Tarbiat Modares University | Kalbassi M.R.,Tarbiat Modares University | Nazari R.M.,Rajaee Sturgeon Hatchery Center
Italian Journal of Zoology | Year: 2013

An important aspect in the management and protection of endangered species from extinction is the determination of normal physiological values, including the blood and seminal plasma properties. This study was undertaken to establish a reference range for six important blood and seminal plasma enzymes. Samples of 20 Acipenser persicus Borodin, 1897 (10 female and 10 male) were analyzed. The reference ranges for the total samples and also for each sex were determined. The activities of measured enzymes in blood and semen were: alkaline phosphatase (ALP, 363.2 ± 25.24 and 12.23 ± 3.66 IU/L), acid phosphatase (ACP, 4.98 ± 0.39 and 1.86 ± 0.71 IU/L), lactate dehydrogenase (LDH, 1978.8 ± 176.5 and 472 ± 138.7 IU/L), creatine kinase (CK, 1149.6 ± 110 and 553.8 ± 119.9 IU/L), aspartate aminotransferase (AST, 51.12 ± 3.5 and 2.29 ± 1.34 IU/L) and alanine aminotransferase (ALT, 14.7 ± 0.89 and 6.9 ± 2.5 IU/L), respectively. There were significant differences between male and female fish in terms of LDH, CK and ALT activity (P < 0.05). To detect relationships among investigated parameters we used the Pearson correlation coefficient; no significant correlations were observed between these parameters in either blood or seminal plasma. © 2013 Unione Zoologica Italiana.


Aramli M.,Tarbiat Modares University | Kalbassi M.,Tarbiat Modares University | Nazari R.,Rajaee Sturgeon Hatchery Center
Reproduction in Domestic Animals | Year: 2013

Contents: The objectives of the present study were to determine ionic and organic composition of seminal plasma, sperm concentration and their relationships in the Persian sturgeon (Acipenser persicus). In this regard, ionic content (Na+, K+, Cl-, Ca2+ and Mg2+) and organic content (total protein, glucose, cholesterol and triglyceride) along with sperm concentration were measured in 17 specimens of the Persian sturgeon. The seminal plasma contained 59.53 ± 2.56 mm/l sodium, 9.1 ± 1.42 mm chloride, 4.72 ± 0.3 mm potassium, 1.45 ± 0.075 mm calcium and 0.7 ± 0.072 mm magnesium. The following organic contents were found: total protein 0.11 ± 0.02 g/dl, glucose 22.18 ± 4.16 mg/dl, cholesterol 6.67 ± 1.04 mg/dl and triglyceride 15.2 ± 0.65 mg/dl. The mean sperm concentration was estimated to be 1.6 ± 0.12 (×109 sperm/ml). A significant relationship was found between sperm concentration and K+ of seminal plasma (r = 0.533, p < 0.05). Significant correlations were observed between ionic contents: Na+ vs Cl- (r = -0.854, p < 0.01) and Mg2+ vs K+ (-0.583, p < 0.05). Also, level of triglyceride was negatively correlated with Mg2+ (r = -0.503, p < 0.05). Presented data could be considered as a complementary study for developing special extenders and protectant solutions for improving artificial fertilization in this valuable species. © 2013 Blackwell Verlag GmbH.


Aramli M.S.,Tarbiat Modares University | Kalbassi M.R.,Tarbiat Modares University | Nazari R.M.,Rajaee Sturgeon Hatchery Center
Comparative Clinical Pathology | Year: 2014

The investigation of blood and seminal plasma biochemical parameters is essential for fish stock conservation and development of artificial propagation methods via extender improvement. In this research, comparison of blood and seminal plasma compounds and their relationship in Persian sturgeon, Acipenser persicus, were studied. Seminal plasma contained 59.53 ± 2.56 mEq/l sodium (Na+), 4.72 ± 0.31 mEq/l potassium (K+), 1.45 ± 0.075 mEq/l chloride (Cl−), 9.1 ± 1.42 mEq/l calcium (Ca2+), 0.7 ± 0.072 mEq/l magnesium (Mg2+), 0.11 ± 0.021 g/dl total protein, 6.67 ± 1.04 mg/dl cholesterol, 15.2 ± 0.65 mg/dl triacylglycerol, and 18.22 ± 4.16 mg/dl glucose. Also, blood biochemical values were determined (mean ± SEM) for Na+ (123.2 ± 1.31 mEq/l), K+ (2.77 ± 0.088 mEq/l), Cl− (97.18 ± 1.23 mEq/l), Ca2+ (6.67 ± 0.24 mEq/l), Mg2+ (1.29 ± 0.074 mEq/l), glucose (198.49 ± 18.03 mg/dl), triacylglycerol (214.22 ± 13.38 mg/dl), total protein (3.62 ± 0.23 g/dl), and cholesterol (127.11 ± 6.94 mg/dl). The mean blood and seminal osmolality values were 244.62 ± 3.66 and 86.9 ± 4.22 mOsm/kg, respectively. Results of the comparison between biochemical parameters of seminal and blood plasma indicated that the concentrations of all parameters of blood plasma were significantly (P < 0.05) higher than seminal plasma except potassium. The statistical Pearson correlation revealed a significant relationship between blood osmolality with levels of Mg++ and Na+ of the seminal plasma (P < 0.05), and glucose levels of blood and seminal plasma (P < 0.01). © 2013, Springer-Verlag London.

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