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Guttikonda V.R.,Mamata Dental College and Hospital | Patil R.,Raja Rajeswari Dental College and Hospital | Kumar G.S.,K S R Institute of Dental science
Journal of Oral and Maxillofacial Pathology | Year: 2014

Aim : To Quantify the DNA single-stranded breaks in the peripheral blood leukocytes (PBLs) of tobacco-habituated individuals with clinically normal mucosa and patients with oral carcinoma.Materials and Methods : Patients who were attending the outpatient department were enrolled in this study. A control group of 30 healthy volunteers included in Group I were selected from various age groups who are not tobacco users in any form. Thirty patients with tobacco habituation but with clinically normal mucosa were included in Group II, while 30 tobacco-habituated patients with oral squamous carcinoma were included in Group III. A biopsy was taken from the representative area and confirmed histologically. Intravenous blood samples were collected from all the groups for evaluation of the extent of DNA damage using ethidium bromide-stained slides under fluorescent microscope. The DNA tail length was calculated by subtracting the diameter from the total length. Twenty-five randomly selected cells per slide were analyzed and mean calculated.Objectives: To evaluate DNA damage levels in PBLs of tobacco-habituated individuals with clinically normal mucosa and patients with oral carcinoma and compare with a control group of healthy volunteers. To evaluate the extent of DNA damage in PBLs using Single Cell Gel Electrophoresis (SCGE) in the above groups.Results : The mean DNA damage levels in patients with tobacco habits were compared with that of the control group and the results were found to be statistically significant. The mean DNA damage level in PBLs between tobacco-habituated patients with normal mucosa and oral cancer patients was found to be statistically significant. The DNA damage in cancer patients was compared with the control group and the results were found to be statistically significant.Conclusion: DNA damage evaluation in PBLs by SCGE technique is a sensitive and reliable indicator of tobacco insult. Source


Singh S.,Raja Rajeswari Dental College and Hospital | Shyamala K.,Raja Rajeswari Dental College and Hospital | Girish H.C.,Raja Rajeswari Dental College and Hospital | Murgod S.,Raja Rajeswari Dental College and Hospital | And 2 more authors.
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2015

The histopathological diagnosis of Oral leukoplakia (OL) and Oral Submucous Fibrosis (OSMF) is based on the subjective evaluation of morphological anomalies within the lesional tissue. Interpretation of dysplasia varies from one pathologist to another. This subjectivity has turned the interest toward applying computer-assisted morphometry to investigate the cellular and nuclear changes in correlation with the histological behaviour of the lesions. To measure the cellular area (CA) and nuclear area (NA) in OL, OSMF and Normal Oral Mucosa (NOM). 2.To measure the nuclear-cytoplasmic ratio (NCR) in basal layer of OL, OSMF and NOM. 3.To compare and correlate the measurements in OL, OSMF and NOM. 45 cases were included in the study with 20 cases of OL, 20 cases of OSMF and 5 cases of NOM. Basal cells were subjected to Morphometric analysis with image analyser software. The cellular and nuclear area were measured and the nuclear-cytoplasmic ratio was calculated in ten fields. Measurements were tabulated and subjected to statistical analysis. The CA & NA in OL, OSMF are larger compared to NOM with a significant P value of 0.015 and 0.40. The Post Hoc Analysis showed a significant difference of 0.023 between CA of OL and OSMF and a significant value of 0.005 between OSMF and NOM. NCR is increased in OL and OSMF when compared to NOM with a significant P value of 0.009 and Post Hoc analysis revealed significant difference of 0.008 between OL & OSMF and between OSMF and NOM. The significant values obtained in the results suggest that histomorphometry can allow objective information to be acquired and can discriminate between the lesions when the diagnosis is ambiguous. Source

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