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Medinīpur, India

Patra A.,Raja Nl Khans Womens College | Mandal A.,Raja Nl Khans Womens College | Roy S.,Raja Nl Khans Womens College | Mandal S.,Raja Nl Khans Womens College | And 2 more authors.
Biomedicine and Preventive Nutrition

Urease positive probiotic Lactobacillus strains were tested for oxidative stress and uremic profile on experimental rat (Wister strains) induced by acetaminophen (APAP) overdose. Experimental rats received acetaminophen interperitoneally at the dose of 500mg/kg/day, continuously for 10days. From 11th day onwards they were orally fed with Lactobacillus fermentum (MTCC 903), Lactobacillus plantarum (MTCC 4462) and Lactobacillus rhamnosus (MTCC 1408) respectively at the dose of 109 CFU/mL/100g of body weight/day for 15days continuously. Plasma, kidney, liver and fecal samples were tested for uremic profile of the sacrificed rats after the experiment. In APAP treated rats, plasma urea, creatinine (Cr), glutamate oxaloacetate transaminase (GOT) and malonaldehyde (MDA) level elevated and catalase (CAT) and super oxide diusmutase (SOD) level declined significantly compared to negative control. However, level of plasma urea, Cr, GOT and MDA in tested rats were significantly lower in comparison to positive control. The uremic profile of the probiotic induced rats was very much comparable with the negative control, even better for some parametric values. Prevention of DNA fragmentation in kidney tissues and reduction of enteric pathogens in feces of Lactobacillus fed rats were noticed. Electrolytes profile of the tested plasma samples were in acceptable range. To sum up, tested urease positive Lactobacillus strains were shown to improve the clinical condition of the acetaminophen induced uremic experimental rats. © 2014 Elsevier Masson SAS. Source

Mandal A.,Raja Nl Khans Womens College | Das K.,Raja Nl Khans Womens College | Roy S.,Raja Nl Khans Womens College | Mondal K.C.,Vidyasagar University | And 2 more authors.
Journal of Nephrology

Objectives: Acetaminophen is a commonly used antipyretic agent which, at high doses, causes renal tubular damage and uremia. Bacteriotherapy affords a promising approach to mitigating uremic intoxication by ingestion of live microbes able to catabolize uremic solutes in the gut. The present study evaluates the nonpathogenic soil-borne urease-positive bacterium Sporosarcina pasteurii (Sp) as a potential urea-targeted component for such an "enteric dialysis" formulation. Methods: Twenty-four albino male rats were randomly divided into 4 groups: The control group (group NC) received distilled water intraperitoneally for 7 days. The positive control group (group U) received 500 mg/kg acetaminophen intraperitoneally for 7 days. The tested group (group UP) was administered Sp at a dosage of 109 cells/day for 5 weeks, after receiving 500 mg/kg per day of acetaminophen intraperitoneally for 7 days. Vehicle control (group VC) received only Sp at a dosage of 109 cells/day for 5 weeks without acetaminophen treatment. Blood, kidney, liver and stool samples were collected after scarification, for biochemical (urea, creatinine, malondialdehyde, superoxide dismutase, catalase, glutamate oxaloacetate transaminase [GOT] and glutamate pyruvate transaminase [GPT] of blood, kidney and liver) tests. Limited fecal analysis was performed. Results: Blood urea nitrogen (urea, creatinine) and toxicity indicators (GOT, GPT) were increased, and antioxidant enzymes were decreased in group U. Blood urea nitrogen and toxicity indicators were reduced, and antioxidant enzymes were increased significantly in the group UP (p<0.05) compared with group U. The number of Sp was increased in Sp-treated groups compared with groups NC and U. Conclusions: The study demonstrated that the bacteria tested reduced blood urea nitrogen levels significantly. © 2012 Società Italiana di Nefrologia - ISSN 1121-8428. Source

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