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Parkash V.,Rain Forest Research Institute ICFRE | Aggarwal A.,Kurukshetra University | Sharma V.,SILB
Journal of Plant Nutrition | Year: 2011

A bio-inoculation experiment was performed on Ruta graveolens L., known as "Garden Rue" seedlings for the biomass production. Seedlings were inoculated with single and in combined mycorrhizal treatments. All inoculated seedlings showed significant biomass production than control seedlings. The phosphorus (P) content was more in seedlings inoculated with consortium of three native VAM fungi followed by Glomus mosseae, Acaulospora laevis and Gigaspora gigantea alone treatments. The control seedlings had the least P content but root P content was more than shoot P content in all treatments. The biomass of shoot and root was also significantly more in seedlings inoculated with consortium followed by G. mosseae, A. laevis and G. gigantea alone treatment. The control seedlings again had low shoot and root biomass. The Biovolume index (Bi) was also high in all inoculated treatments than non-inoculated control but consortium treatment (7.98) had at par Bi than rest of inoculation treatments. The quality index (Qi) value was also high in mixed consortium (1.42) and A. laevis treatment (1.42) each respectively than G. mosseae (1.38) and G. gigantea (1.29) treatments. Control seedlings had low value of Qi (1.27). The present study indicates that G. mosseae, A. laevis, G. gigantea are the best strains of VAM symbionts when mixed together for inoculating the R. graveolens to get higher yield of biomass. A. laevis is also good strain of VAM fungi for the same purpose. The G. gigantea alone proved least effective strain for inoculation purpose in R. graveolens but when mixed together in consortium of native VAM fungi as described above executed good results for enhancing the biomass production. © Taylor & Francis Group, LLC.

Parkash V.,Rain Forest Research Institute ICFRE | Aggarwal A.,Kurukshetra University
Indian Journal of Traditional Knowledge | Year: 2010

The paper documents the traditional knowledge of medicinal plants that are used by the indigenous villagers residing in remote foot-hill areas of Himachal Pradesh in household remedies. An ethnobotanical survey was conducted on lower foothills of Himachal Pradesh during 2007-08. About ten different ethnobotanical plant species were recorded for their medicinal uses and for other remedial purposes by the local inhabitants. There were seven families of which Asteraceae and Amaranthaceae families were mostly exploited by the people. Abrus precatorius is found vulnerable in Hamirpur district due to its excessive exploitation for various purposes by the local contractors. Crotolaria juncea is not only used as medicinal plant but it is also used as green manure in the fields. During survey, it is also found that some plant species such as Abrus precatorius, Eclipta alba, Deeringia amaranthoides and Physalis minima require in situ as well as ex situ conservation in the area for maintaining future germplasm source.

Tanwar A.,Kurukshetra University | Aggarwal A.,Kurukshetra University | Parkash V.,Rain Forest Research Institute ICFRE
New Zealand Journal of Crop and Horticultural Science | Year: 2014

A glasshouse pot experiment was conducted to investigate the effect of two species of arbuscular mycorrhizal fungi (Glomus intraradices and Acaulospora laevis) and Pseudomonas fluorescens under three levels of superphosphate fertilizer (half dose, 40 kg/ha; recommended dose, 80 kg/ha; and double dose, 160 g/ha) on the growth and yield of broccoli (Brassica oleracea var. italica). There was negligible colonization of broccoli roots by the mycorrhizal fungi, demonstrating that they have no role in the growth of this plant. However, the strain of P. fluorescens used (MTCC No. 103) was found to have a potential role in enhancing the growth, phosphatase activity, chlorophyll content, nutrient uptake and yield of broccoli when combined with the recommended dose of fertilizer. By contrast, P. fluorescens combined with a half or double dose of fertilizer led to poor growth and delayed head formation. This suggests that root inoculation with P. fluorescens (MTCC No. 103) will improve plant growth, nutrient uptake and yield of broccoli when combined with the recommended dose of superphosphate fertilizer. © 2014 The Royal Society of New Zealand.

Tanwar A.,Kurukshetra University | Aggarwal A.,Kurukshetra University | Parkash V.,Rain Forest Research Institute ICFRE
Journal of Central European Agriculture | Year: 2013

A pot experiment was conducted to test the influence of sugarcane bagasse (fibrous waste left over after sugarcane juice) as a substrate for the inoculum production of Funneliformis mosseae in terms of AM root colonization, spore number and AM colonization pattern using onion as host plant. Their effect on growth performance of onion was also recorded in terms of increase in plant height, above ground fresh and dry weight, root length, root fresh and dry weight. The experiment was a 3×4 factorial design employing three forms of bagasse (fresh, dry and compost) and their four different concentrations (without substrate, 25 g/pot, 50 g/pot and 100 g/pot). The results showed that the compost bagasse promoted higher AM root colonization and sporulation, followed by dry and fresh bagasse. Maximum AM spores, vesicles, arbuscules and 100 per cent colonized roots were detected in onion plants supplemented with 25 g compost bagasse. This treatment also influenced significant increase in plant growth. Although, increasing substrate concentration proved stimulatory to AM fungus as well as onion plant growth but highest concentration (100 g) proved inhibitory. Hence, compost bagasse can be exploited for the multiplication of F. mosseae by farmers as it is a cost effective method of production.

Tanwar A.,Kurukshetra University | Aggarwal A.,Kurukshetra University | Yadav A.,Kurukshetra University | Parkash V.,Rain Forest Research Institute ICFRE
Biological Agriculture and Horticulture | Year: 2013

This investigation reports a novel and low-cost method of inoculum production of arbuscular mycorrhizal (AM) fungus, Funneliformis mosseae (sensu Glomus mosseae), using an organic medium. The experiment is a 3 × 3 × 4 factorial design employing three hosts, maize (Zea mays L.), lemon grass (Cymbopogon nardus (L.) Rendle) and palmarosa (Cymbopogan martini (Roxb.) Wats.), three forms of sugarcane bagasse substrate (fresh, dry and compost) and four different concentrations of each substrate (without substrate, 25, 50 and 100 g pot-1). Mass multiplication of F. mosseae was measured in terms of AM spore number and per cent root colonization. The plant growth was monitored in terms of plant height, above-ground fresh and dry weights and root fresh and dry weights. All three grasses tested varied in their tendency to be colonized by F. mosseae and in spore formation. Maize was found to be the most appropriate host followed by lemon grass and palmarosa. In the case of maize, a positive relationship was observed between spore number and root colonization rate and compost bagasse with highest concentration, 100 g, resulted in the greatest multiplication of F. mosseae. Maize with 100 g compost bagasse, lemon grass with 25 g of dry and 50 g of compost bagasse and palmarosa with 100 g compost and fresh bagasse showed greatest increase in plant growth. © 2013 Taylor & Francis.

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