Gradosova I.,Ustav Klinicke Biochemie A Diagnostiky |
Svejkovska K.,Ustav Klinicke Biochemie A Diagnostiky |
Zivna H.,Vivarium A Radioizotopove Laboratore |
Zivny P.,Ustav Klinicke Biochemie A Diagnostiky |
And 3 more authors.
Osteologicky Bulletin | Year: 2011
Objective: The study aimed at assessing the effect of a frequently used antihypertensive, metoprolol (a β1-selective blocker of adrenergic receptors devoid of internal sympathomimetic activity), on bone metabolism in healthy adult male Wistar rats. Material and methods: Healthy adult male Wistar rats (240 ± 10 g) were administered metoprorol orally once daily by gastric tube for 8 weeks. The rats were divided into two groups of eight animals. The control group (CON) was given water for injection (0.2 mL/100 g BW) and the study group received metoprolol (MET; 0.5 mg in 0.2 mL of water for injection/100 g BW) in the form of a suspension. In the serum, a bone resorption marker carboxy-terminal telopeptide of collagen type I (ICTP) and bone formation markers osteocalcin (OC), alkaline phosphatase bone isoenzyme (BALP) and amino-terminal propeptide of type I procollagen (PINP) were assessed using the ELISA method. A bone homogenate from the proximal tibia was used to measure BMP-2 by the Western blot. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry (DXA). Mechanical resistance of bone tissue was tested on the femurs. Results: In the MET group, resistance to pressure of the femoral neck decreased by 21 % after 8 weeks, as compared with the CON group. Expression of BMP-2 in the bone increased. No significant changes were observed in BMD and bone marker concentrations. Conclusion: The results suggest that metoprolol affects bone tissue in healthy adult male Wistar rats by decreasing the resistance of bone tissue in the femoral neck and by increased expression of BMP-2 in the proximal tibia.