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Bangalore, India
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Kumar P.M.,Padmashree Institute of Management and Sciences | Venkataranganna M.V.,Connexios Life science Pvt. Ltd. | Manjunath K.,Bangalore University | Ashok G.,Radiant Research Services Pvt. Ltd.
Journal of Intercultural Ethnopharmacology | Year: 2016

Aims: The present study was undertaken to evaluate the effect of methanolic leaf extract of Gymnema sylvestre (MLGS) on glucose transport (GLUT) and insulin resistance in vitro. Materials and Methods: Peroxisome proliferator-activated receptor-gamma (PPAR-γ) and GLUT-4 expression were assessed in L6 myotubes for concluding the GLUT activity, and adiponectin and leptin expression was studied in 3T3 L1 murine adipocyte cell line to determine the effect of MLGS (250-750 μg/ml) on insulin resistance. Results: The findings of the experiments have demonstrated a significant and dose-dependent increase in glucose uptake in all the tested concentrations of MLGS, further the glucose uptake activity of MLGS (750 μg/ml) was at par with rosiglitazone (50 μg/ml). Concomitantly, MLGS has shown enhanced GLUT-4 and PPAR-γ gene expressions in L6 myotubes. Furthermore, cycloheximide (CHX) had completely abolished the glucose uptake activity of MLGS when coincubated, which further confirmed that glucose uptake activity of MLGS was linked to enhanced expression of GLUT-4 and PPAR-γ. In addition, in another experimental set, MLGS showed enhanced expression of adiponectin and leptin, thus confirms the ameliorative effect of MLGS on insulin resistance. Conclusion: These findings suggest that MLGS has an enhanced glucose uptake activity in L6 myotubes, and ameliorate the insulin resistance in 3T3 L1 murine adipocyte cell line in vitro. © SAGEYA.


Godavarthi A.,Radiant Research Services Pvt. Ltd.
Pharmacognosy Journal | Year: 2013

Aim To evaluate in vitro hepatoprotective activity of ethanolic extract from Corchorus depressus (CDEE) against CCl4 induced toxicity in HepG2 cell line. Methods In vitro cytotoxicity and hepatoprotective potential of CDEE were evaluated using HepG2 cells. Based on the cytotoxicity assay, CDEE (50, 100, 200 μg/ml) was assessed for hepatoprotective potential against CCl4 induced toxicity by monitoring cell viability, aspartate aminotransferase (AST), alanine aminotransaminase (ALT), lactate dehydrogenase (LDH) leakage, lipid peroxidation (LPO) and glutathione level (GSH). Results The results indicated that CCl4 treatment caused a significant decrease in cell viability. In addition, the toxin treatment initiated lipid peroxidation (LPO), caused leakage of enzymes like transaminase (AST & ALT) and LDH with a significant decrease in GSH levels in HepG2 cells. It was observed that CDEE effectively alleviated the changes induced by CCl4 in a concentration-dependent manner. Conclusion Our study revealed that CDEE has potent cytoprotective effect against CCl4 induced toxicity in HepG2 cell line and which may be attributed to decrease in CCl4 induced reactive oxygen species levels and resultant oxidative stress. © 2013, Phcog.Net, Published by Reed Elsevier India Pvt. Ltd. All rights reserved.


Ethnopharmacological relevance The whole plant of Fagonia schweinfurthii (Hadidi) Hadidi (Family: Zygophyllaceae) is used in variety of diseases including hepatic ailments in deserts and dry areas of India. Aim of the study To evaluate antioxidant and hepatoprotective activity of ethanolic extract from Fagonia schweinfurthii (Hadidi) Hadidi (FSEE) in carbon tetrachloride (CCl 4) induced hepatotoxicity in HepG2 cell line and rats. Materials and methods In vitro antioxidant activity was determined by DPPH, ABTS radicals and hydrogen peroxide methods. In vitro cytotoxicity and hepatoprotective potential of FSEE were evaluated using HepG2 cells. Based on the cytotoxicity assay, FSEE (50, 100, 200 μg/ml) was assessed for hepatoprotective potential against CCl4 induced toxicity in HepG2 cell line by monitoring cell viability, aspartate aminotransferase (AST), alanine aminotransaminase (ALT), lactate dehydrogenase (LDH) leakage, lipid peroxidation (LPO) and glutathione level (GSH). Further, in vivo hepatoprotective activity of FSEE was evaluated against CCl4 induced hepatotoxicity in male Wistar albino rats. Rats were pre-treated with FSEE (200 mg, 400 mg kg-1 day-1 p.o.) for 7 days followed by a single dose of CCl4 (1.0 ml/kg, i.p.) on 8th day. Silymarin was used as positive control. After 24 h of CCl 4 administration, various biochemical parameters like aspartate aminotransferase (AST), alanine aminotransaminase (ALT), alkaline phosphatase (ALP), total bilirubin (TB) and total protein (TP) levels were estimated in serum. The antioxidant parameters like superoxide dismutase (SOD) activity, catalase (CAT) activity, glutathione (GSH) content and malondialdehyde (MDA) level in the liver homogenate were determined. Histopathological changes in the liver of different groups were also studied. Results The FSEE possessed strong antioxidant activity in vitro. The results indicated that CCl4 treatment caused a significant decrease in cell viability. The CCl 4-induced changes in the HepG2 cells were significantly ameliorated by treatment of the FSEE. FSEE significantly prevented CCl4 induced elevation of AST, ALT, ALP, TB, and CCl4 induced decrease in total protein in rats. FSEE treated rat liver anti-oxidant parameters (SOD, CAT, MDA and GSH,) were significantly antagonized for the pro-oxidant effect of CCl 4. Histopathological studies also supported the protective effect of FSEE. Conclusion The results of this study revealed that FSEE has significant hepatoprotective activity. This effect may be due to the ability of the extract to inhibit lipid peroxidation and increase in the anti-oxidant enzymatic activity. © 2013 Elsevier Ireland Ltd.


Bellamakondi P.K.,Radiant Research Services Pvt Ltd | Godavarthi A.,Radiant Research Services Pvt Ltd | Ibrahim M.,Nizam Institute of Pharmacy | Kulkarni S.,Radiant Research Services Pvt Ltd | And 2 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

Caralluma species have been reported for various traditional claims; the scientific data for possible activities have not yet been studied thoroughly. However the toxicity of a plant has to be studied for further exploration of various biological activities. Extracts were studied for their toxicity by MTT and trypan blue dye exclusion models against a panel of cancer, normal origin cell lines and EAC cells. The extracts found to be moderately toxic and showed dose dependent response.


Bellamakondi P.K.,Radiant Research Services Pvt Ltd | Godavarthi A.,Radiant Research Services Pvt Ltd | Ibrahim M.,Nizam Institute of Pharmacy
BioImpacts | Year: 2014

Introduction: Hyperglycemia is a serious health problem prevailing in diabetes patients. Treatment for hyperglycemia by various oral anti-hyperglycemic drugs have associated with side effects, hence there is growing awareness towards the use of herbal products due to their efficacy, minimal side effects and relatively low costs. This study is designed to evaluate anti-hyperglycemic activity of Caralluma umbellata Haw, which is used as a traditional medicinal plant all over India through in vitro studies. Methods: Methanolic, aqueous and hydro methanolic extracts of Caralluma umbellata were prepared and studied for their anti hyperglycemic activity. The extracts were evaluated for glucose uptake in L6 myotubes in vitro. In addition, the inhibitory activity against alpha amylase and pancreatic lipase was also measured. Results: The methanolic extract (MCU) was found to have significant glucose uptake. Further, MCU was also found to have promising role in inhibiting alpha amylase and pancreatic lipase. Conclusion: The results of present study shows Caralluma umbellata has potential antidiabetic property, thus providing a further scope for study in animal model and understanding the mechanism of action. © 2014 The Author(s).


Kumar P.M.,Padmashree Institute of Management and Sciences | Venkataranganna M.V.,Connexios Life science Pvt Ltd | Manjunath K.,Bangalore University | Ashok G.,Radiant Research Services Pvt Ltd.
Chinese Journal of Natural Medicines | Year: 2014

The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria (MFMC) on PPARγ (Peroxisome Proliferator Activated Receptor gamma) and GLUT-4 (Glucose transporter-4) with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL-1 were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC (500 μg·mL-1) was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARγ gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide (CHX), which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARγ and GLUT-4 in vitro. © 2014 China Pharmaceutical University.


PubMed | Manipal University India, Radiant Research Services Pvt. Ltd., Dr. Babasaheb Ambedkar Marathwada University and International Medical University
Type: Journal Article | Journal: Antioxidants (Basel, Switzerland) | Year: 2016

Hypericum is a well-known plant genus in herbal medicine. Hypericum mysorense (Family: Hypericaceae), a plant belonging to the same genus, is well known in folklore medicine for its varied therapeutic potential.The aim of the present study was to investigate the different parts of the plant for antioxidant and hepatoprotective properties.The methanol extracts of Hypericum mysorense prepared from various parts of the plant were tested in vitro for their free radical scavenging activity against ABTS() (diammonium salt), DPPH() (1,1-diphenyl-2-picrylhydrazyl), NO(), O(-) and ()OH radicals, using standard systems of assays. The total antioxidant capacity, total phenolic and total flavonoid content of the extracts were analyzed. Further, the leaf and flowering top extracts were tested for their in vivo antioxidant and hepatoprotective activities on Wistar rats using a carbon tetrachloride-induced hepatic injury model.The leaf and flowering top extract showed potent antioxidant activity and also possessed highest total phenolic and flavonoid content. The antioxidant activity and the total phenolic and flavonoid content present in these extracts showed a good correlation. The leaf and flowering top extracts at 200 mg/kg restored aspartate amino transferase (ASAT), alanine amino transferase (ALAT), alkaline phosphatase (ALP), total bilirubin and protein levels significantly in CCl-intoxicated rats. The tested extracts also showed a significant (p < 0.001) reduction in 2-thiobarbituric acid reactive substance (TBARS) levels with an increase in SOD and CAT levels. The histopathology of liver did not show any toxicity after the treatment with the extracts. The active extracts were standardized using two marker compounds, hyperoside and rutin, which were isolated from the plant by HPLC. HPLC studies revealed that the maximum concentration of hyperoside and rutin is present in the flowering top extract.


PubMed | Padmashree Institute of Management and Sciences, Bangalore University, Connexios Life science Pvt. Ltd. and Radiant Research Services Pvt. Ltd.
Type: Journal Article | Journal: Journal of intercultural ethnopharmacology | Year: 2016

The present study was undertaken to evaluate the effect of methanolic leaf extract of Gymnema sylvestre (MLGS) on glucose transport (GLUT) and insulin resistance in vitro.Peroxisome proliferator-activated receptor-gamma (PPAR-) and GLUT-4 expression were assessed in L6 myotubes for concluding the GLUT activity, and adiponectin and leptin expression was studied in 3T3 L1 murine adipocyte cell line to determine the effect of MLGS (250-750 g/ml) on insulin resistance.The findings of the experiments have demonstrated a significant and dose-dependent increase in glucose uptake in all the tested concentrations of MLGS, further the glucose uptake activity of MLGS (750 g/ml) was at par with rosiglitazone (50 g/ml). Concomitantly, MLGS has shown enhanced GLUT-4 and PPAR- gene expressions in L6 myotubes. Furthermore, cycloheximide (CHX) had completely abolished the glucose uptake activity of MLGS when co-incubated, which further confirmed that glucose uptake activity of MLGS was linked to enhanced expression of GLUT-4 and PPAR-. In addition, in another experimental set, MLGS showed enhanced expression of adiponectin and leptin, thus confirms the ameliorative effect of MLGS on insulin resistance.These findings suggest that MLGS has an enhanced glucose uptake activity in L6 myotubes, and ameliorate the insulin resistance in 3T3 L1 murine adipocyte cell line in vitro.


PubMed | Radiant Research Services Pvt. Ltd. and Olive Lifesciences Pvt. Ltd.
Type: Journal Article | Journal: Journal of traditional and complementary medicine | Year: 2016

Coleus forskohlii Briq. has been used traditionally for the treatment of several ailments since antiquity in Ayurveda. In the present study, an approach has been made to evaluate the effect of C.forskohlii and its major constituents on cytochrome P450 (CYP3A, CYP2B, and CYP2C) mRNA expression in rat hepatocytes. To gain better understanding of the herb-drug interaction potential of the chemical constituents present in C.forskohlii, the extract was subjected to column chromatography followed by standardization with respect to forskolin, 1-deoxyforskolin, and 1,9-dideoxyforskolin using reversed-phase high-performance liquid chromatography (RP-HPLC). Hepatocytes were treated with extracts, fractions, and phytoconstituents, followed by extraction and purification of total mRNA. Study of mRNA expression was carried out through reverse transcription polymerase chain reaction, followed by agarose gel electrophoresis. Results revealed that the test substances did not show any significant mRNA expression compared to the control against CYP3A, CYP2B, and CYP2C. Positive controls such as dexamethasone and rifampin showed significantly high (p<0.001) induction potential compared to the control. It can be concluded that C.forskohlii and its major constituents may not be involved in CYP450 induction-based drug interaction.


Hullatti K.,KLE University | Pathade N.,KLE University | Mandavkar Y.,KLE University | Godavarthi A.,Radiant Research Services Pvt. Ltd | Biradi M.,KLE University
Pharmaceutical Biology | Year: 2013

Context: The ethanol extracts and their fractions of three Indian medicinal plants, Ervatamia coronaria (Jacq.) Stapf, (Apocynaceae), Mimosa pudica L. (Mimosaceae) and Caesalpinia bonduc (L.) Roxb. (Caesalpiniaceae) were tested for their cytotoxic activity in the brine shrimp lethality (BSL) bioassay and in various cancer cell lines. The plants were selected based on their traditional use in the treatment of cancer/tumors. Objectives: To investigate the in vitro cytotoxicity of Ervatamia coronaria, Mimosa pudica and Caesalpinia bonduc. Materials and methods: Ethanolic extracts and their fractions of E. coronaria, M. pudica and C. bonduc were subjected to cytotoxicity studies using BSL bioassay method with concentrations of 10, 50, 100, 500 and 1000μg/ml. The alkaloid fraction of E. coronaria with significant cytotoxicity in BSL bioassay was subjected to in vitro cytotoxicity studies with HT-29, A-549, HepG-2, MCF-7 and L-6 cell lines at concentrations of 12.5, 25, 50, 100 and 200μg/ml and a DNA fragmentation study using the HT-29 cell line. Results: The alkaloid fractions of E. coronaria and M. pudica showed significant cytotoxicity with LC50 values of 65.83 and 85.10μg/ml in the BSL bioassay, respectively. The purified alkaloid fraction of E. coronaria exhibited highest cytotoxicity in HT-29, A-549 and MCF-7 cell lines with IC50 values of 32.5, 47.5 and 72.5μg/ml, respectively, and induced DNA fragmentation in the HT-29 cell line at a concentration of 65μg/ml. Conclusion: The alkaloid fraction of E. coronaria exhibited significant cytotoxicity. Alkaloids such as ervatamine, apparicine and coronaridine that were earlier reported may be responsible for this activity. © 2013 Informa Healthcare USA, Inc.

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