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Kumar P.M.,Padmashree Institute of Management and Sciences | Venkataranganna M.V.,Connexios Life science Pvt Ltd | Manjunath K.,Bangalore University | Ashok G.,Radiant Research Services Pvt Ltd
Chinese Journal of Natural Medicines | Year: 2014

The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria (MFMC) on PPARγ (Peroxisome Proliferator Activated Receptor gamma) and GLUT-4 (Glucose transporter-4) with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL-1 were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC (500 μg·mL-1) was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARγ gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide (CHX), which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARγ and GLUT-4 in vitro. © 2014 China Pharmaceutical University.

Hullatti K.,KLE University | Pathade N.,KLE University | Mandavkar Y.,KLE University | Godavarthi A.,Radiant Research Services Pvt Ltd | Biradi M.,KLE University
Pharmaceutical Biology | Year: 2013

Context: The ethanol extracts and their fractions of three Indian medicinal plants, Ervatamia coronaria (Jacq.) Stapf, (Apocynaceae), Mimosa pudica L. (Mimosaceae) and Caesalpinia bonduc (L.) Roxb. (Caesalpiniaceae) were tested for their cytotoxic activity in the brine shrimp lethality (BSL) bioassay and in various cancer cell lines. The plants were selected based on their traditional use in the treatment of cancer/tumors. Objectives: To investigate the in vitro cytotoxicity of Ervatamia coronaria, Mimosa pudica and Caesalpinia bonduc. Materials and methods: Ethanolic extracts and their fractions of E. coronaria, M. pudica and C. bonduc were subjected to cytotoxicity studies using BSL bioassay method with concentrations of 10, 50, 100, 500 and 1000μg/ml. The alkaloid fraction of E. coronaria with significant cytotoxicity in BSL bioassay was subjected to in vitro cytotoxicity studies with HT-29, A-549, HepG-2, MCF-7 and L-6 cell lines at concentrations of 12.5, 25, 50, 100 and 200μg/ml and a DNA fragmentation study using the HT-29 cell line. Results: The alkaloid fractions of E. coronaria and M. pudica showed significant cytotoxicity with LC50 values of 65.83 and 85.10μg/ml in the BSL bioassay, respectively. The purified alkaloid fraction of E. coronaria exhibited highest cytotoxicity in HT-29, A-549 and MCF-7 cell lines with IC50 values of 32.5, 47.5 and 72.5μg/ml, respectively, and induced DNA fragmentation in the HT-29 cell line at a concentration of 65μg/ml. Conclusion: The alkaloid fraction of E. coronaria exhibited significant cytotoxicity. Alkaloids such as ervatamine, apparicine and coronaridine that were earlier reported may be responsible for this activity. © 2013 Informa Healthcare USA, Inc.

Hebbani Nagarajappa S.,Industrial Research Ltd. | Pandit S.,Industrial Research Ltd. | Divanji M.,Industrial Research Ltd. | Mariyanna B.,Industrial Research Ltd. | And 2 more authors.
Journal of Traditional and Complementary Medicine | Year: 2016

Coleus forskohlii Briq. has been used traditionally for the treatment of several ailments since antiquity in Ayurveda. In the present study, an approach has been made to evaluate the effect of C. forskohlii and its major constituents on cytochrome P450 (CYP3A, CYP2B, and CYP2C) mRNA expression in rat hepatocytes. To gain better understanding of the herb-drug interaction potential of the chemical constituents present in C. forskohlii, the extract was subjected to column chromatography followed by standardization with respect to forskolin, 1-deoxyforskolin, and 1,9-dideoxyforskolin using reversed-phase high-performance liquid chromatography (RP-HPLC). Hepatocytes were treated with extracts, fractions, and phytoconstituents, followed by extraction and purification of total mRNA. Study of mRNA expression was carried out through reverse transcription polymerase chain reaction, followed by agarose gel electrophoresis. Results revealed that the test substances did not show any significant mRNA expression compared to the control against CYP3A, CYP2B, and CYP2C. Positive controls such as dexamethasone and rifampin showed significantly high (p < 0.001) induction potential compared to the control. It can be concluded that C. forskohlii and its major constituents may not be involved in CYP450 induction-based drug interaction. © 2014 Center for Food and Biomolecules, National Taiwan University.

Bellamakondi P.K.,Radiant Research Services Pvt Ltd | Godavarthi A.,Radiant Research Services Pvt Ltd | Ibrahim M.,Nizam Institute of Pharmacy | Kulkarni S.,Radiant Research Services Pvt Ltd | And 2 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2014

Caralluma species have been reported for various traditional claims; the scientific data for possible activities have not yet been studied thoroughly. However the toxicity of a plant has to be studied for further exploration of various biological activities. Extracts were studied for their toxicity by MTT and trypan blue dye exclusion models against a panel of cancer, normal origin cell lines and EAC cells. The extracts found to be moderately toxic and showed dose dependent response.

Bellamakondi P.K.,Radiant Research Services Pvt Ltd | Godavarthi A.,Radiant Research Services Pvt Ltd | Ibrahim M.,Nizam Institute of Pharmacy
BioImpacts | Year: 2014

Introduction: Hyperglycemia is a serious health problem prevailing in diabetes patients. Treatment for hyperglycemia by various oral anti-hyperglycemic drugs have associated with side effects, hence there is growing awareness towards the use of herbal products due to their efficacy, minimal side effects and relatively low costs. This study is designed to evaluate anti-hyperglycemic activity of Caralluma umbellata Haw, which is used as a traditional medicinal plant all over India through in vitro studies. Methods: Methanolic, aqueous and hydro methanolic extracts of Caralluma umbellata were prepared and studied for their anti hyperglycemic activity. The extracts were evaluated for glucose uptake in L6 myotubes in vitro. In addition, the inhibitory activity against alpha amylase and pancreatic lipase was also measured. Results: The methanolic extract (MCU) was found to have significant glucose uptake. Further, MCU was also found to have promising role in inhibiting alpha amylase and pancreatic lipase. Conclusion: The results of present study shows Caralluma umbellata has potential antidiabetic property, thus providing a further scope for study in animal model and understanding the mechanism of action. © 2014 The Author(s).

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