Hande P.,Gurunanak Khalsa College |
Ajitkumar B.S.,Gurunanak Khalsa College |
Rane S.,Ruia College |
Hate M.,Ruia College |
Charegaonkar A.,Ruia College
International Journal of Pharma and Bio Sciences | Year: 2015
Leucas aspera linn. is commonly called as 'Pandharpheda' and is widely distributed throughout south India, having many medicinal properties. A sensitive, simple and accurate HPTLC method has been established for identification and quantification of beta-sitosterol from leucas aspera linn. whole plant powder. Quantification was carried out on pre-coated plate of HPTLC silica gel 60 F254 using mobile phase (toluene: ethyl acetate: formic acid, 12:7:1,v/v/v). CAMAG scanner IV set at visible light (520nm) for detection and quantification. The percentage concentrations of the beta-sitosterol were found to be 0.0672.Response was linear over the range of 0.1μg to 0.5μg with 0.66 and 0.314 of %CV respectively.CAMAG TLC-MS interface was used for the confirmation of m/z values of beta-sitosterol in standard as well as sample. The m/z value was found to be 397(M+H+-H2O). The method i.e. HPTLC and TLC-MS together provides a rapid and secure method for detection, identification and quantification of biomarker betasitosterol.
Patil P.G.,Ruia College |
Nachane R.P.,Central Institute for Research on Cotton Technology
International Conference and Workshop on Emerging Trends in Technology 2011, ICWET 2011 - Conference Proceedings | Year: 2011
Creep has been known and studied for textile materials for decades. In comparison, a newly observed phenomenon of inverse creep seems not to have received much attention. A new instrument has been fabricated to measure creep and inverse creep in textile materials particularly yarns. Creep and Inverse creep measurements on a few Cotton Yarns at different levels of stress have been studied, using the new instrument and results are reported in the present paper. Copyright © 2011 ACM.