R and nter for Fermentation Engineering of Hebei Province

Shijiazhuang, China

R and nter for Fermentation Engineering of Hebei Province

Shijiazhuang, China

Time filter

Source Type

Gao W.-H.,Hebei University of Science and Technology | Gao W.-H.,R and nter for Fermentation Engineering of Hebei Province | Liu B.,Hebei University of Science and Technology | Liu B.,R and nter for Fermentation Engineering of Hebei Province | And 6 more authors.
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | Year: 2014

To prepare myclobutanil molecularly imprinted polymer, a method was established for the choice of the appropriate functional monomer and its dosage. UV spectra was applied to study the combination form, the effect intensity, the optimal concentration ratio and the numbers of binding sites between myclobutanil and methyl acrylic acid (MAA) or acrylamide (AM) functional monomer. The results showed that hydrogen-bonding interaction could be formed between myclobutanil and methyl acrylic acid (MAA) or acrylamide (AM) functional monomer. The π electron of the triazole ring conjugated double bond in myclobutanil could transit to π* conjugate antibonding orbital when it absorbed energy. The formation of hydrogen bond could make π→π* absorption band transit. Maximum absorption wavelength produced red shift with the increase in the functional monomer concentration in the system. The research revealed that the optimal concentration ratios between myclobutanil and the two monomers were c(M):c(MAA)=1:4, c(M):c(AM)=1:2. Myclobutanil and the both the functional monomers had the bonding ability, and strong bonding force. The prepared molecularly imprinted polymer using AM as a functional monomer had better stability and specificity of recognition for myclobutanil.


Sun P.,Hebei University of Science and Technology | Sun P.,R and nter for Fermentation Engineering of Hebei Province | Liu J.-G.,Hebei University of Science and Technology | Liu J.-G.,R and nter for Fermentation Engineering of Hebei Province | And 3 more authors.
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | Year: 2010

The solubilities of avermectins in different alcohol-water systems are very important for the industrial production of avermectins. In this paper, the solubilities of avermectins in binary solvents of methanol-water (methanol content 100%~60%) and ethanol-water (ethanol content 100%~70%) were determined by equilibrium method in the temperature range of 30~70°C. The experimental results were correlated with mathematic model of empirical polynomial equation and the semiempirical equation, respectively, and both the correlation coefficients are above 98%. It was found that, in the binary solvent of methanol-water mixture, the higher the water content, the lower the avermectin solubility. At 32°C, the solubility of avermectin in 90% methanol-water mixture decreases by 67% of the solubility in absolute methanol. While the avermectin solubility in 95% ethanol-water mixture only decreases by 28% of the solubility in pure ethanol, this decrease is much lower than that in the methanol-water system.


Liu B.,Hebei University of Science and Technology | Liu B.,R and nter for Fermentation Engineering of Hebei Province | Liu B.,Zhangjiakou Cigarette Factory Co Ltd. | Wang J.,Hebei University of Science and Technology | And 5 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2014

To prepare a myclobutanil molecularly imprinted polymer (MMIP) and study its specific recognition ability, MMIP was prepared by the method of bulk polymerization under different ratios between template molecular and functional monomer, using myclobutanil as template molecule, methacrylate acid (MAA) as functional monomer. The influence of different pore-foaming agents on the interaction between template molecular and functional monomer was investigated, the effect of the prepared polymer using different ratios template molecular and functional monomer on the adsorption content of myclobutanil was also studied. Adsorption property of myclobutanil was examined by static adsorption test and analyzed by Scatchard analysis. The results indicated that the maximum absorption wavelength of myclobutanil could produce red shift and the red shifts were 4 nm and 2 nm, respectively, and the absorption intensity increased using acetonitrile and tetrahydrofuran as pore-foaming agent. Through the Scatchard analysis, it showed that myclobutanil template molecule and functional monomer MAA formed one types of binding sites, the dissociation constants of the binding sites was 1.36 mmol/L, respectively. Tetrahydrofuran and acetonitrile were more suitable for the preperation of MMIP. The molecularly imprinted polymer can be used as the separation media of myclobutanil with high affinity and good specific recognition ability. ©, 2014, Chinese Institute of Food Science and Technology. All right reserved.


Wang X.-X.,Hebei University of Science and Technology | Wang X.-X.,R and nter for Fermentation Engineering of Hebei Province | Zhao Z.-H.,Hebei University of Science and Technology | Zhao Z.-H.,R and nter for Fermentation Engineering of Hebei Province | And 4 more authors.
Bioprocess and Biosystems Engineering | Year: 2011

Wastewater from avermectin production is refractory with high output, high chemical oxygen demand (COD) concentration and high cost further. The current wastewater treatment technology, with little reuse of the organic material, needs high dilution ratio during treatment. Yeast single cell protein was produced from the wastewater of avermectins fermentation in this research. First, the yeast strain (H-1) which show enough tolerance to avermectins residue was screened from the wastewater, and it was identified as being most closely related to Candida tropicalis (100%, EF120592.1) using 18S rDNA gene sequence analysis. Second, its growth characteristics in the avermectins wastewater were studied. The dry biomass reached the maximal point of 10 g/L, the COD removal was up to 66.67%, and avermectins removal in the wastewater was 99.48% at the optimal condition that it was liquid volume 50 ml (250 ml flask), pH 4.0, temperature 30°C, inoculum volume 10% (V/V) and fermentation period 20 h. Third, the nutritional contents of dry yeast powder were determined, in which the water content was 8.12%, ash content was 5.18% and the crude protein was 40.02%. The dry yeast powder from avermectin waste liquor was promising to be used as a raw material or nitrogen source for commercial production of avermectins. The project was economically feasible by primary cost accounting. © 2011 Springer-Verlag.


Zhao C.,Hebei University of Science and Technology | Zhao C.,R and nter for Fermentation Engineering of Hebei Province | Pang J.,Hebei University of Science and Technology | Pang J.,R and nter for Fermentation Engineering of Hebei Province | And 2 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2015

Objective:To develop a method of the determination three triazole fungicides in food by molecularly imprinted solid phase extraction-high performance liquid chromatography. Methods: Molecularly imprinted polymers (MIP) were prepared by the way of bulk polymerization, using bitertanol, myclobutanil and diniconazole as template molecule, respectively, methacrylate acid (MAA) and acrylamide(AM) as functional monomer, ethylene glycol dimethacrylate (ED-MA) as crosslinker. The MIPs of bitertanol, myclobutanil and diniconazole were mixed according to mass ratio of 1:1:1, they were used as the solid-phase extraction (SPE) columns which were used in the pre-treatment of sample. The residues of bitertanol, myclobutanil and diniconazole in the sample were determined by high performance liquid chromatography. Results: The baseline separation among bitertanol, myclobutanil and diniconazole was obtained within 5 min. Under low and high concentration added levels, the average recoveries were between 90.6% and 92.7%, and the RSDs were between 1.2% and 3.3%(n=5). The detection limits were 0.1 µg/g for bitertanol and 0.2 µg/g for myclobutanil and diniconazole. Conclusion: It showed that this method had fast analysis speed, good sensitivity and reproducibility, it was suitable for simultaneous detecting bitertanol, myclobutanil and diniconazole residues in food. ©, 2015, Chinese Institute of Food Science and Technology. All right reserved.

Loading R and nter for Fermentation Engineering of Hebei Province collaborators
Loading R and nter for Fermentation Engineering of Hebei Province collaborators