R and D Ultrafast Lasers Ltd

Budapest, Hungary

R and D Ultrafast Lasers Ltd

Budapest, Hungary
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Krolopp A.,Hungarian Academy of Sciences | Krolopp A.,R and D Ultrafast Lasers Ltd | Csakanyi A.,Hungarian Academy of Sciences | Haluszka D.,Hungarian Academy of Sciences | And 5 more authors.
Optics InfoBase Conference Papers | Year: 2016

A novel, Yb-fiber laser based, handheld 2PEF/SHG microscope imaging system is introduced being suitable for in vivo imaging of murine skin at an average power level as low as 5 mW at 200 kHz sampling rate. © OSA 2016.


Grosz T.,University of Szeged | Grosz T.,R and D Ultrafast Lasers Ltd | Kovacs A.P.,University of Szeged | Szipocs R.,R and D Ultrafast Lasers Ltd | Szipocs R.,Hungarian Academy of Sciences
Optics InfoBase Conference Papers | Year: 2016

Broadband dielectric beam steering mirrors are key components for nonlinear microscopes comprising tunable Ti:sapphire lasers. We characterize dispersion of a few mirror samples by spectral interferometry and show how it affects imaging quality. © OSA 2016.


Toke E.R.,Genetic Immunity Kft | Toke E.R.,EMMUNITY Inc | Lorincz O.,Genetic Immunity Kft | Lorincz O.,EMMUNITY Inc | And 25 more authors.
Gene Therapy | Year: 2014

There is no clinically available cancer immunotherapy that exploits Langerhans cells (LCs), the epidermal precursors of dendritic cells (DCs) that are the natural agent of antigen delivery. We developed a DNA formulation with a polymer and obtained synthetic 'pathogen-like' nanoparticles that preferentially targeted LCs in epidermal cultures. These nanoparticles applied topically under a patch-elicited robust immune responses in human subjects. To demonstrate the mechanism of action of this novel vaccination strategy in live animals, we assembled a high-resolution two-photon laser scanning-microscope. Nanoparticles applied on the native skin poorly penetrated and poorly induced LC motility. The combination of nanoparticle administration and skin treatment was essential both for efficient loading the vaccine into the epidermis and for potent activation of the LCs to migrate into the lymph nodes. LCs in the epidermis picked up nanoparticles and accumulated them in the nuclear region demonstrating an effective nuclear DNA delivery in vivo. Tissue distribution studies revealed that the majority of the DNA was targeted to the lymph nodes. Preclinical toxicity of the LC-targeting DNA vaccine was limited to mild and transient local erythema caused by the skin treatment. This novel, clinically proven LC-targeting DNA vaccine platform technology broadens the options on DC-targeting vaccines to generate therapeutic immunity against cancer. © 2014 Macmillan Publishers Limited.


Kolonics A.,Hungarian Academy of Sciences | Kolonics A.,R and D Ultrafast Lasers Ltd | Csiszovszki Z.,Genetic Immunity Kft | Csiszovszki Z.,EMMUNITY Inc. | And 8 more authors.
Experimental Dermatology | Year: 2014

Epidermal Langerhans cells (LCs) function as professional antigen-presenting cells of the skin. We investigated the LC-targeting properties of a special mannose-moiety-coated pathogen-like synthetic nanomedicine DermaVir (DV), which is capable to express antigens to induce immune responses and kill HIV-infected cells. Our aim was to use multiphoton laser microscopy (MLM) in vivo in order to visualize the uptake of Alexa-labelled DV (AF546-DV) by LCs. Knock-in mice expressing enhanced green fluorescent protein (eGFP) under the control of the langerin gene (CD207) were used to visualize LCs. After 1 h, AF546-DV penetrated the epidermis and entered the eGFP-LCs. The AF546-DV signal was equally distributed inside the LCs. After 9 h, we observed AF546-DV signal accumulation that occurred mainly at the cell body. We demonstrated in live animals that LCs picked up and accumulated the nanoparticles in the cell body. © 2014 John Wiley & Sons A/S.


Csati A.,Hungarian Academy of Sciences | Antal P.,Hungarian Academy of Sciences | Szipics R.,Hungarian Academy of Sciences | Szipics R.,R and D Ultrafast Lasers Ltd
Advances in Optical Materials, AIOM 2012 | Year: 2012

An inherently synchronized Yb-fiber laser based extension unit for femtosecond pulse, broadly tunable Ti-sapphire lasers is introduced, which is well suited for coherent anti-Stokes Raman scattering microscopy. © 2012 OSA.


Csati D.,Hungarian Academy of Sciences | Szipocs R.,Hungarian Academy of Sciences | Szipocs R.,R and D Ultrafast Lasers Ltd
Fiber Lasers and Applications, FILAS 2012 | Year: 2012

Mode-locked operation of an all-fiber, all-normal dispersion Yb-fiber ring laser is actively optimized by a two-channel polarization controller according to the measured, normalised, single-sideband noise power. © 2012 OSA.


Szipocs R.,Hungarian Academy of Sciences | Szipocs R.,RandD Ultrafast Lasers Ltd. | Antal P.G.,Hungarian Academy of Sciences | Szigligeti A.,Hungarian Academy of Sciences | And 2 more authors.
Optics InfoBase Conference Papers | Year: 2011

We report on a broadly tunable, long-cavity, low-pump-threshold, pulsed Ti:Sapphire laser. The laser delivers nearly transform limited ~300 fs, ~10 nJ pulses at 22 MHz repetition rate being ideal for nonlinear microscopy. © 2011 OSA.


Kolonics A.,Hungarian Academy of Sciences | Kolonics A.,R and D Ultrafast Lasers Ltd | Csati D.,Hungarian Academy of Sciences | Antal P.,Hungarian Academy of Sciences | And 2 more authors.
Biomedical Optics, BIOMED 2012 | Year: 2012

An inherently synchronized Yb-fiber amplifier based extension unit for femtosecond pulse, broadly tunable Ti-sapphire lasers is introduced, which is well suited for coherent anti-Stokes Raman scattering microscopy. © 2012 OSA.


Haluszka D.,Semmelweis University | Haluszka D.,Hungarian Academy of Sciences | Lorincz K.,Semmelweis University | Molnar G.,University of Szeged | And 7 more authors.
Microscopy Research and Technique | Year: 2015

Nonlinear microscopy techniques are being increasingly used to perform in vivo studies in dermatology. These methods enable us to investigate the morphology and monitor the physiological process in the skin by the use of femtosecond lasers operating in the red, near-infrared spectral range (680-1,300 nm). In this work we used two different techniques that require no labeling: second harmonic generation (SHG) for collagen detection and coherent anti-Stokes Raman scattering (CARS) to assess lipid distribution in genetically obese murine skin. Obesity is one of the most serious public health problems due to its high and increasing prevalence and the associated risk of type 2 diabetes and cardiovascular diseases. Other than these diseases, nearly half of patients with diabetes mellitus suffer from dermatological complications such as delayed wound healing, foot ulcers and several other skin changes. In our experiment we investigated and followed the effects of obesity on dermal collagen alterations and adipocyte enlargement using a technique not reported in the literature so far. Our results indicate that the in vivo SHG and ex vivo CARS imaging technique might be an important tool for diagnosis of diabetes-related skin disorders in the near future. © 2015 Wiley Periodicals, Inc.


Krolopp A.,Hungarian Academy of Sciences | Krolopp A.,R and D Ultrafast Lasers Ltd | Csakanyi A.,Hungarian Academy of Sciences | Haluszka D.,Hungarian Academy of Sciences | And 8 more authors.
Biomedical Optics Express | Year: 2016

A novel, Yb-fiber laser based, handheld 2PEF/SHG microscope imaging system is introduced. It is suitable for in vivo imaging of murine skin at an average power level as low as 5 mW at 200 kHz sampling rate. Amplified and compressed laser pulses having a spectral bandwidth of 8 to 12 nm at around 1030 nm excite the biological samples at a ~1.89 MHz repetition rate, which explains how the high quality two-photon excitation fluorescence (2PEF) and second harmonic generation (SHG) images are obtained at the average power level of a laser pointer. The scanning, imaging and detection head, which comprises a conventional microscope objective for beam focusing, has a physical length of ~180 mm owing to the custom designed imaging telescope system between the laser scanner mirrors and the entrance aperture of the microscope objective. Operation of the all-fiber, all-normal dispersion Yb-fiber ring laser oscillator is electronically controlled by a two-channel polarization controller for Q-switching free mode-locked operation. The whole nonlinear microscope imaging system has the main advantages of the low price of the fs laser applied, fiber optics flexibility, a relatively small, light-weight scanning and detection head, and a very low risk of thermal or photochemical damage of the skin samples. © 2016 Optical Society of America.

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