Kruger A.,University of Cambridge |
Kruger A.,Max Planck Institute for Molecular Genetics |
Vowinckel J.,University of Cambridge |
Mulleder M.,University of Cambridge |
And 6 more authors.
EMBO Reports | Year: 2013
Cells counteract oxidative stress by altering metabolism, cell cycle and gene expression. However, the mechanisms that coordinate these adaptations are only marginally understood. Here we provide evidence that timing of these responses in yeast requires export of the polyamines spermidine and spermine. We show that during hydrogen peroxide (H 2 O 2) exposure, the polyamine transporter Tpo1 controls spermidine and spermine concentrations and mediates induction of antioxidant proteins, including Hsp70, Hsp90, Hsp104 and Sod1. Moreover, Tpo1 determines a cell cycle delay during adaptation to increased oxidant levels, and affects H 2 O 2 tolerance. Thus, central components of the stress response are timed through Tpo1-controlled polyamine export. © 2013 EUROPEAN MOLECULAR BIOLOGY ORGANIZATION.
Farrell D.J.,Quotient Bioresearch |
Krause K.M.,Theravance |
Diagnostic Microbiology and Infectious Disease | Year: 2011
The in vitro activity of telavancin was determined for 94 diverse Staphylococcus spp. Telavancin had MIC 90 values of 0.5 μg/mL for methicillin-susceptible, methicillin-resistant, and vancomycin-susceptible Staphylococcus aureus, and coagulase-negative staphylococci isolates. Telavancin MICs were 0.5-1 μg/mL for vancomycin-intermediate S. aureus isolates and 2-4 μg/mL for vancomycin-resistant S. aureus strains. © 2011 Elsevier Inc.
Lewis J.C.M.,Wildlife Vets International |
Teale P.,LCG Group |
Webber G.,Quotient Bioresearch |
Sear J.W.,University of Oxford |
Taylor P.M.,Taylor Monroe
Veterinary Journal | Year: 2014
Serious post-operative neurological complications of unknown aetiology are reported in tigers after immobilisation using tiletamine and zolazepam. These complications may arise from the persistent effects of tiletamine or active metabolites of tiletamine or zolazepam. Concentrations of tiletamine, zolazepam and some metabolites were measured using high performance liquid chromatography-mass spectrometry in plasma from captive tigers (n=8) and leopards (n=9; an unaffected species, for comparison) during anaesthesia for routine clinical procedures. The zolazepam:tiletamine (Z:T) ratio was calculated. Peak concentrations occurred at 9-33min and ranged from 83.5 to 379.2ng/mL for tiletamine and 301.1 to 1239.3ng/mL for zolazepam after correction for dose by weight. There were no significant differences between tigers and leopards. The Z:T ratio was generally <5 and did not differ between species. In both tigers and leopards, zolazepam metabolism appeared to be primarily via demethylation. There was evidence for hydroxylation in leopards, but much less in tigers than leopards. No major differences between the species in parent pharmacokinetics were identified. The metabolism of tiletamine could not be defined with any degree of certainty for either species. © 2014 Elsevier Ltd.
Kingsley C.,Quotient Bioresearch
Sp2 | Year: 2010
The Bioanalytical Sciences division of Quotient Bioresearch was set up in response to the needs of the marketplace and has developed a range of technologies and services in the areas of immunogenicity testing for biotherapeutics; measuring immune response to new drugs coming to market; cell-based neutralising antibody assays; bioanalytical testing; and other services related to biopharmaceuticals.
Agency: Cordis | Branch: FP7 | Program: CP-FP | Phase: KBBE-2008-2-4-02 | Award Amount: 4.40M | Year: 2009
Biocides have been in use for hundreds of years for antisepsis, disinfection and preservation. Despite this widespread and ever increasing use most bacterial and fungal species remain susceptible to biocides. The dramatic increase and spread of resistance to antibiotics linked to reports of co- and cross-resistance between antibiotics and biocides raised speculations on potential hazard of biocide use. The overarching question which BIOHYPO is aimed to address is: has the use of biocides contributed to the development and spread of clinically significant antibiotic resistance in human pathogens? Core of BIOHYPO are a high throughput screening approach on collections of thousands of well characterized microorganisms and an interactive web based data analysis platform. Phenotypic screening for reduced susceptibility to biocides, detection of novel resistance genes and mobile elements, and screening for their molecular epidemiology and metagenomics will be accompanied by methodological innovation for testing, risk evaluation and registration of biocides. Altogether BIOHYPO aims to provide solid data and analysis to direct future issuing of guidelines for safe environmental, medical and industrial use of biocides.