Chaitanya Krishna A.,Quest Life science Pvt Ltd |
Vignesh R.,Quest Life science Pvt Ltd |
Chelladurai R.,Quest Life science Pvt Ltd |
Patil R.S.,Thermo Fisher Scientific
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012
A suitable, highly specific and sensitive liquid chromatography tandem mass spectrometric method has been developed and validated for the determination of Ofloxacin from 100μL of human plasma by Protein Precipitation. Ciprofloxacin was used as an internal standard. Quantified by the transition, 362.200→261.420, 332.205→282.500 for Ofloxacin and Ciprofloxacin respectively and detected by TSQ Quantum Ultra triple quadrupole mass spectrometer. Detection was carried out by using ESI source in positive polarity. Chromatographic separation of analyte and internal standard were carried out by reverse phase C18 column at the flow rate of 0.500mL/min with mobile phase of Acetonitrile: 0.02% Formic acid (60:40) v/v. The assay of Ofloxacin was linear over the range of 20.133 ng/mL to 2001.800 ng/mL with a precision of ≤0.45 % and ≤6.88 % respectively, Mean extraction recovery obtained was 89.84%. Samples were stable at room temperature for 6 hrs and also stable at four freeze-thaw cycle. The aim is to develop a suitable, highly specific, and sensitive analytical method for the quantitation of Ofloxacin in the low nanogram range in human plasma.
Parthasarathi T.R.,Quest Life science Pvt. Ltd |
Srinivas T.S.,Quest Life science Pvt. Ltd |
Sri V.M.,Quest Life science Pvt. Ltd |
Ram S.S.,Quest Life science Pvt. Ltd |
And 2 more authors.
International Journal of Pharma and Bio Sciences | Year: 2012
A novel isocratic reverse-phase high performance liquid-chromatography method for determination of asenapine maleate was developed and validated after optimization of various chromatographic conditions. Samples were separated on a waters x-terra C18 (100 mm × 4.6 mm, 3.5 μ) analytical column. The mobile phase used was acetonitrile: 0.1M phosphate buffer (pH 3.2) 65:35%v/v operated at 30 °C column oven temperature was pumped at a flow rate of 1.0 mL min-1 and the column eluents were monitored at a wavelength of 272 nm. When sample was injected into the Finnigan surveyor high performance liquid-chromatography system through Finnigan surveyor auto-sampler injector, separation was achieved within 5.0 min. The present method was demonstrated and it was validated with the acceptable values for selectivity, linearity (within the expected concentration range (10-50 μg mL-1; r2 > 0.999)), recovery (>95%), precision (%RSD < 2.0), sensitivity (limit of detection: 1.85 μg mL-1 and lower limit of quantification: 2.34 μg mL-1), robustness, and ruggedness.
Tamilselvi M.,Quest Life science Pvt Ltd |
Srinivas A.,Quest Life science Pvt Ltd
International Journal of Pharmaceutical and Clinical Research | Year: 2014
In a double-blinded, randomized, clinical trial lasting two weeks in 20 patients with skin infections (Eczema, Dermatitis, and pruritus), inflammatory and Pruritic manifestations of corticosteroid responsive dermatoses Pruritus, Eczema and Atopic Dermatitis were treated and compared between 1% w/w hydrocortisone cream and reference 1% w/w cutisoft hydrocortisone acetate cream and reductions of the basic criteria, i.e. itching, erythema and scaling, were evaluated. Effects were compared using Visual Analogue Scale, Patients compliance and Physician Global Evaluation of efficacy. All treatment regimens significantly reduced itching, erythema and scaling after 4 visits. The patients were assessed on day 01 (Visit 1), day 05 + 01 (Visit 2), day 10 + 01 (Visit 3) and on day 14 + 01 (Visit 4) for the analyses of infection. The basic criteria scores were decreased from visit 1 to visit 4. All patients were well tolerated. The results of therapeutic outcome proved the better results for test cream over reference cuti soft cream, the statistical analysis based on Generalized Linear model shown that both the products are clinically equivalent in terms of anti-inflammatory effect of hydrocortisone cream in this study.