Quest life science Private Ltd

Chennai, India

Quest life science Private Ltd

Chennai, India

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Patil R.S.,Swami Ramanand Teerth Marathwada University | Patil S.R.,Swami Ramanand Teerth Marathwada University | Makone S.S.,Swami Ramanand Teerth Marathwada University | Krishna C.A.,Quest life science Private Ltd | Thirugnanam P.E.,Quest life science Private Ltd
Current Trends in Biotechnology and Pharmacy | Year: 2010

A simple, rapid and sensitive method using an isocratic Liquid chromatography coupled with Tandem mass spectrometry was developed and validated for the assay of Tacrolimus in the Human Blood. The Mass transition of Tacrolimus and Sirolimus (Internal standard) were M/z 821.7/ 768.7 and M/Z 931.6/864.6 in ESI Negative ionization. Linearity was observed between the Tacrolimus concentration and the peak area ratio from 0.52 to 61.00 ng/mL with r2 value of 0.99. Blood samples containing Tacrolimus were extracted with Zinc sulphate and methanol to precipitate proteins followed by SPE. The observed recovery of Tacrolimus was 66.7%. The intraday and inter-day accuracy ranged from 91.3 110.5% and from 93.9 - 100.6% respectively, at Low, middle and high level concentrations. The method will be used in the determination of the pharmacokinetic parameters of Tacrolimus after oral administration of Tacrolimus formulation in human blood.


Patil R.S.,Thermofisher Scientific India Pvt. Ltd | Chaitanya Krishna A.,Quest life science Private Ltd | Ravi Prakash P.V.D.L.S.,Quest life science Private Ltd | Patil S.R.,Quest life science Private Ltd
Current Trends in Biotechnology and Pharmacy | Year: 2012

A simple, rapid and sensitive method using an isocratic Liquid chromatography coupled with Tandem mass spectrometry was developed and validated for the assay of nitrofurantoin in the Human Plasma. The Mass transition of nitrofurantoin and losartan (Internal standard) were M/z 237.000/151.800 and M/Z 421.300/ 179.274 in ESI Negative ionization. Linearity was observed between the nitrofurantoin concentration and the peak area ratio from 10.107 to 999.900ng/mL with r2 value of 0.99. Plasma samples containing nitrofurantoin were extracted with ethyl acetate. The observed recovery of nitrofurantoin was 80.2 %. The intraday and inter-day accuracy ranged from 83.61 to 107.16% and from 93.13to 103.02% respectively, at Low, middle and high level concentrations. The method will be used in the determination of the pharmacokinetic parameters of nitrofurantoin after oral administration of nitrofurantoin formulation in human Plasma.

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