Queensland Fertility Group

Queensland, Australia

Queensland Fertility Group

Queensland, Australia

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Gosalvez J.,Autonomous University of Madrid | Coppola L.,Centro Medico Biologico | Fernandez J.L.,Complejo Hospitalario Universitario runa CHUAC | Lopez-Fernandez C.,Autonomous University of Madrid | And 8 more authors.
Reproductive BioMedicine Online | Year: 2017

The nitroblue tetrazolium (NBT) reaction as a tracer of oxidative stress was examined in 707 ejaculates from seven clinics. Semen was initially surveyed by classifying the NBT reaction using a pre-established rank for the Oxisperm® test based on three colourimetric levels: L1, low (n = 141 [20%]); L2, medium (n = 538 [76%]) and L3, high (n = 28 [4%]). L3 was indicative of a high level of superoxide anions. Halosperm® chromatin dispersion assay was used to analyse samples of ejaculates 30 min after ejaculation; no difference was found in DNA fragmentation of L1 or L3; L3 category semen samples incubated for 24 h at 37oC showed a significantly faster rate (P < 0.001) of DNA damage than those in L1. The NBT reaction was further characterized in the ejaculates of 100 patients to determine the relative contribution of seminal plasma, spermatozoa, or both. Seminal plasma was the most significant fraction of •O2 - localization, whereas sperm fractions generated detectable reactive oxygen species in only 32% of the ejaculates. Formazan precipitates were primarily associated with the sperm mid-piece and seminal leukocytes; however, not all spermatozoa stained positive to formazan and not all leukocytes presented with equivalent production of superoxide anions. © 2017 Reproductive Healthcare Ltd.


Walls M.,Fertility Specialists of WA | Zuvela E.,Fertility Specialists of WA | Ayres C.,Demeter Laboratories | Sherrin D.,Queensland Fertility Group | And 14 more authors.
Asian Pacific Journal of Reproduction | Year: 2012

Objective: To undertake a multi-centre study to maximize the number of Makler chambers used. Methods: A total of 15 laboratories participated with 31 Makler chambers. A suspension of latex beads was prepared to a concentration of 20 millions per milliliter, and 0.5 mL aliquots distributed to each participating laboratory. They measured the concentration on their Makler chamber(s) used for routine semen analysis by adding 3, 4, 5, 7 and 10 μL volumes of bead suspension to the chamber. Results: There was no difference in within-chamber analysis of the bead concentration according to the volume of bead suspension applied within the range of 3-10 μL (F4,14=2.634, P=0.056). However, the between-chamber effects were significantly different (F30,124=4.937, P=0.000), and 24/31 (77.5%) chambers tested had an average bias>10% compared to the target bead concentration. Conclusions: A volume of 3-10 μL added to Makler counting chambers does not influence the concentration measured of latex beads, but the between-chamber variability and positive bias seen would suggest that other sources of error are present which are yet to be identified. © 2012 Hainan Medical College.


Matson P.L.,Sir Charles Gairdner Hospital | Matson P.L.,Murdoch University | Myssonski K.,Canberra Fertility Center | Yovich S.,Pivet Medical Center | And 4 more authors.
Reproductive Biology | Year: 2010

A multi-centre study was undertaken to: a/determine the density of human semen, and b/assess the validity of measuring semen volume either volumetrically or gravimetrically. Semen samples from four clinical categories (azoospermia following vasectomy, azoospermia without vasectomy, oligozoospermia (<20×106/ml) and normozoospermia (>20×106/ml)) had similar densities (one-way ANOVA: F(3,180) =1.25, not signifi cant), being close to 1.0 g/ml when taken to one decimal place. Measurement of semen volume was then made with either a graduated pipette or by weighing and assuming a density of 1 g/ml. A comparison of the two methods gave an excellent correlation, with a gradient of 1.0571 and a coeffi cient of determination (R2) of 0.98 (p <0.0001). However, it was noted that the aspiration of the ejaculate in to a graduated pipette underestimated the volume by approximately 0.2 ml, but in an inconsistent manner making the use of a set correction factor inappropriate. The estimation of volume to one decimal place by weighing the collection container before and after ejaculation, assuming a density of 1 g/ml, would seem to be a viable alternative although the density of a small number of samples may deviate from this assumption. Whilst the relatively small underestimation of volume with a pipette is unlikely to have clinical signifi cance, the known reporting of inaccurate results by a laboratory is contrary to the philosophy and key principles of quality management. © 2010 by the Society for Biology of Reproduction.


Delatycki M.B.,Austin Health | Delatycki M.B.,Murdoch Childrens Research Institute | Burke J.,Tasmanian Clinical Genetics Services | Christie L.,Hunter Genetics | And 13 more authors.
Twin Research and Human Genetics | Year: 2014

Since the discovery in 1989 that mutations in cystic fibrosis transmembrane conductance regulator (CFTR) underlie cystic fibrosis (CF), the most common life shortening genetic disorder in Caucasians, it has been possible to identify heterozygous mutation carriers at risk of having affected children. The Human Genetics Society of Australasia has produced a position statement with recommendations in relation to population-based screening for CF. These include: (1) that screening should be offered to all relatives of people with or carriers of CF (cascade testing) as well as to all couples planning to have children or who are pregnant; (2) the minimum CFTR mutation panel to be tested consists of 17 mutations which are those mutations that are associated with typical CF and occur with a frequency of 0.1% or higher among individuals diagnosed with CF in Australasia; (3) that genetic counselling is offered to all couples where both members are known to have one or two CFTR mutations and that such couples are given the opportunity to meet with a physician with expertise in the management of CF as well as a family/individual affected by the condition. © 2014 The Author(s).


Sanchez-Calabuig M.-J.,Instituto Nacional Of Investigacion Y Tecnologia Agraria Y Alimentaria Inia | Lopez-Fernandez C.,Autonomous University of Madrid | Martinez-Nevado E.,Zoo Aquarium | Perez-Gutierrez J.F.,Complutense University of Madrid | And 5 more authors.
Reproduction in Domestic Animals | Year: 2014

Over the last two decades, there have been significant advances in the use of assisted reproductive technology for genetic and reproductive management of captive dolphin populations, including evaluation of sperm DNA quality. This study validated a customized sperm chromatin dispersion test (SCDt) for the bottlenose dolphin (Tursiops truncatus) as a means of assessing sperm DNA damage both in the field and in the laboratory. After performing the SCDt, two different sperm morphotypes were identified: (i) sperm with fragmented DNA showed large haloes of dispersed DNA fragments emerging from a compact sperm nucleoid core and (ii) sperm containing non-fragmented DNA displayed small compact haloes surrounded by a dense core of non-dispersed DNA and protein complex. Estimates of sperm DNA fragmentation by means of SCDt were directly comparable to results obtained following a two-tailed comet assay and showed a significant degree of correlation (r = 0.961; p < 0.001). This investigation also revealed that the SCDt, with minor modifications to the standard protocol, can be successfully conducted in the field using a LED florescence microscopy obtaining a high correlation (r = 0.993; p = 0.01) between the data obtained in the laboratory and in the field. © 2014 Blackwell Verlag GmbH.


Sanchez-Calabuig M.J.,Instituto Nacional Of Investigacion Y Tecnologia Agraria Y Alimentaria Inia | Lopez-Fernandez C.,Autonomous University of Madrid | Johnston S.D.,University of Queensland | Blyde D.,University of Queensland | And 4 more authors.
Reproduction in Domestic Animals | Year: 2015

Contents: Sperm DNA fragmentation is one of the major causes of infertility; the sperm chromatin dispersion test (SCDt) evaluates this parameter and offers the advantage of species-specific validated protocol and ease of use under field conditions. The main purpose of this study was to evaluate sperm DNA fragmentation dynamics in both fresh and post-thaw bottlenose dolphin sperm using the SCDt following different cryopreservation protocols to gain new information about the post-thaw differential sperm DNA longevity in this species. Fresh and cryopreserved semen samples from five bottlenose dolphins were examined for sperm DNA fragmentation dynamics using the SCDt (Halomax®). Sperm DNA fragmentation was assessed immediately at collection and following cryopreservation (T0) and then after 0.5, 1, 4, 8, 24, 48 and 72 h incubation at 37°C. Serially collected ejaculates from four dolphins were frozen using different cryopreservation protocols in a TES-TRIS-fructose buffer (TTF), an egg-yolk-free vegetable lipid LP1 buffer (LP1) and human sperm preservation medium (HSPM). Fresh ejaculated spermatozoa initially showed low levels of DNA fragmentation for up to 48 h. Lower Sperm DNA fragmentation (SDF) was found in the second fresh ejaculate compared to the first when more than one sample was collected on the same day (p < 0.05); this difference was not apparent in any other seminal characteristic. While there was no difference observed in SDF between fresh and frozen-thawed sperm using the different cryopreservation protocols immediately after thawing (T0), frozen-thawed spermatozoa incubated at 37°C showed an increase in the rate of SDF after 24 h. Sperm frozen in the LP1{sound recording copyright} buffer had higher levels (p < 0.05) of DNA fragmentation after 24- and 48-h incubation than those frozen in TTF or HSPM. No correlation was found between any seminal characteristic and DNA fragmentation in either fresh and/or frozen-thawed samples. © 2015 Blackwell Verlag GmbH.


PubMed | Materials Hospital, Monash Medical Center, Royal Hobart Hospital, St Vincents Hospital and 10 more.
Type: Journal Article | Journal: Pathology | Year: 2016

Chromosome microarrays are an essential tool for investigation of copy number changes in children with congenital anomalies and intellectual deficit. Attempts to standardise microarray testing have focused on establishing technical and clinical quality criteria, however external quality assessment programs are still needed. We report on a microarray proficiency testing program for Australasian laboratories. Quality metrics evaluated included analytical accuracy, result interpretation, report completeness, and laboratory performance data: sample numbers, success and abnormality rate and reporting times. Between 2009 and 2014 nine samples were dispatched with variable results for analytical accuracy (30-100%), correct interpretation (32-96%), and report completeness (30-92%). Laboratory performance data (2007-2014) showed an overall mean success rate of 99.2% and abnormality rate of 23.6%. Reporting times decreased from >90 days to <30 days for normal results and from >102 days to <35 days for abnormal results. Data trends showed a positive correlation with improvement for all these quality metrics, however only report completeness and reporting times reached statistical significance. Whether the overall improvement in laboratory performance was due to participation in this program, or from accumulated laboratory experience over time, is not clear. Either way, the outcome is likely to assist referring clinicians and improve patient care.


PubMed | University of Auckland, Genea Fertility, IVF Australia, University of New South Wales and Queensland Fertility Group
Type: Journal Article | Journal: Human reproduction (Oxford, England) | Year: 2016

Have ART live birth rates improved in Australia over the last 12 years?There were striking improvements in per-cycle live birth rates observed for frozen/thaw embryo transfers, blastocyst transfer and single embryo transfer (SET), while live birth rates following ICSI were lower than IVF for non-male factor infertility in most years.ART and associated techniques have become the predominant treatment of infertility over the past 30 years in most developed countries. However, there are differences in ART laboratory and clinical practices, and success rates worldwide. Australia has one of the highest ART utilization rates and lowest multiple birth rates in the world, thus providing a unique setting to investigate the contribution of common ART strategies in an unrestricted population of patients to ART success rates.A retrospective cohort study of 585 065 ART treatment cycles performed in Australia between 2002 and 2013 using the Australian and New Zealand Assisted Reproduction Database (ANZARD).An unrestricted population of all women who underwent autologous ART treatment between 2002 and 2013. Visual descriptive analysis was used to assess the trends in ART procedures by the calendar years. Adjusted odds ratios (aORs) of a live birth for four common ART techniques were calculated after controlling for important confounders including female age, infertility diagnosis, stage of the embryo (blastocyst versus cleavage stage), type of embryo (fresh versus thawed), fertilization method (IVF versus ICSI) and number of embryos transferred (SET versus multiple embryos).The overall live birth rate per embryo transfer increased from 19.2% in 2002 to 23.3% in 2013 (21.9-24.3% for fresh embryo transfers and 14.6-23.3% for frozen/thaw embryo transfers). This occurred concurrently with an increase in SET from 29.7% to 78.9%, and an increase in the average age of women undergoing treatment from 35.0 to 35.9 years. Individuals who had a frozen/thaw embryo transfer cycle in 2002 had 43% (aOR: 0.57, 95% CI: 0.53-0.61) reduced odds of a live birth compared with a fresh embryo transfer cycle. This contrasted with 16% (aOR: 0.84, 95% CI: 0.80-0.98) reduced odds of a live birth from frozen/thaw embryo transfer cycles in 2013. In 2013, the odds of blastocyst transfer resulting in a live birth were more than twice as great as for cleavage stage transfer (aOR 2.01, 95% CI: 1.92-2.11). The adjusted odds of live birth per SET compared with multiple embryo transfer increased significantly over the last 12 years, from a 38% reduced odds of a live birth follow SET in 2002 (aOR: 062, 95% CI: 0.57-0.67) compared to an 8% reduced odds in 2013 (aOR: 0.92, 95% CI: 0.87-0.98). The aOR of a live birth using ICSI compared to IVF in non-male factor patients was lower in most years bringing into question its widespread use.This is a retrospective cohort analysis and cannot confirm causality. High-level evidence on the effectiveness of particular ART techniques, particularly ICSI and blastocyst culture, requires prospective randomized controlled trials or detailed statistical analysis using large-scale data that counts for fertilization failure, embryo loss, prognostic factors and cycle characteristics.The most striking improvements in ART success rates in Australia have been observed for frozen/thaw embryo transfers, blastocyst transfer and SET. Further studies of the role of ICSI in non-male factor infertility and blastocyst transfer success rates that take into account embryo loss are needed.No funding was received to undertake this study. The authors declare that they do not have competing interests with this study.NA.


Harrison K.,Queensland Fertility Group | Darling N.,Queensland Fertility Group | Vargas K.,Queensland Fertility Group | Irving J.,Queensland Fertility Group | And 3 more authors.
Australian and New Zealand Journal of Obstetrics and Gynaecology | Year: 2015

In assisted reproduction, knowledge of the presence of transmissible disease assists diagnosis and permits appropriate risk minimisation. The overall incidence was lowest in the Brisbane full-cost clinic and highest in the Springwood low-cost clinic. Male partners predominated over females, particularly in the low-cost clinic. Hepatitis C was the most commonly detected infection with the highest incidence in the low-cost clinic. HIV was the least commonly detected infection amongst those tested. © 2015 The Royal Australian and New Zealand College of Obstetricians and Gynaecologists.


McDowell S.,Queensland Fertility Group Research Foundation | Harrison K.,Queensland Fertility Group | Kroon B.,Queensland Fertility Group Research Foundation | Kroon B.,University of Queensland | And 4 more authors.
Fertility and Sterility | Year: 2013

Objective: To determine if men with malignancy have increased sperm DNA fragmentation compared with men presenting for sperm donation. Design: Retrospective observational study. Setting: Tertiary-level fertility center. Patient(s): Eighty-nine men with cancer presenting for prophylactic semen cryopreservation and 35 men presenting for sperm donation. Intervention(s): None. Main Outcome Measure(s): Sperm DNA fragmentation index (DFI) measured by sperm chromatin assay. Result(s): The mean sperm DFI in men with a diagnosis of cancer, 9.88% (95% confidence interval [CI] 7.84%-12.44%), did not differ from that found in men presenting for sperm donation 10.46% (95% CI 8.68%-11.80%). There were no significant differences in mean sperm DFI within cancer subgroups or when comparing testicular and nontesticular cancers. Subgroup analysis lacked statistical power. Men with testicular cancer have significantly reduced sperm concentration compared with both control subjects and men with nontesticular cancer. Conclusion(s): In our study population there was no difference in sperm DFI between men undergoing prophylactic semen cryopreservation and men presenting for sperm donation. Sperm DFI assessment has limited utility in the routine evaluation of men presenting for semen cryopreservation. © 2013 American Society for Reproductive Medicine, Published by Elsevier Inc.

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