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Bayside, NY, United States

Park K.M.,Queensborough Community College
ASEE Annual Conference and Exposition, Conference Proceedings | Year: 2015

This paper presents how to bring current high technologies in engineering classes with the use of 3D printers in design and fabrication of electric go-kart parts. Students in Mechanical Engineering Technology Club with interests in automotive technology as well as manufacturing technology were given the opportunities to design and build electrically powered go-kart to learn product design, prototyping and manufacturing. Using 3D printers, students designed and built number of different go-kart body parts, including a steering wheel column cover and a front nose wing pieces. In the process, students learned to design for manufacturing, build within maximum build envelope of the 3D printers, assembly for parts, and resolve fitment issues. Additional parts to be designed include side skirts, motor and battery covers, and a rear wing. The outcome of such learning experiences from this project can be expanded to interdisciplinary project oriented courses for engineering students to enhance their learning experiences. © American Society for Engineering Education, 2015.

Guo Y.,State University of New York at Stony Brook | Golebiewska U.,Queensborough Community College | Scarlata S.,State University of New York at Stony Brook
Biophysical Journal | Year: 2011

Cardiomyocytes have a complex Ca 2+ behavior and changes in this behavior may underlie certain disease states. Intracellular Ca 2+ activity can be regulated by the phospholipase Cβ-Gα q pathway localized on the plasma membrane. The plasma membranes of cardiomycoytes are rich in caveolae domains organized by caveolin proteins. Caveolae may indirectly affect cell signals by entrapping and localizing specific proteins. Recently, we found that caveolin may specifically interact with activated Gα q, which could affect Ca 2+ signals. Here, using fluorescence imaging and correlation techniques we show that Gα q-Gβγ subunits localize to caveolae in adult ventricular canine cardiomyoctyes. Carbachol stimulation releases Gβγ subunits from caveolae with a concurrent stabilization of activated Gα q by caveolin-3 (Cav3). These cells show oscillating Ca 2+ waves that are not seen in neonatal cells that do not contain Cav3. Microinjection of a peptide that disrupts Cav3-Gα q association, but not a control peptide, extinguishes the waves. Furthermore, these waves are unchanged with rynaodine treatment, but not seen with treatment of a phospholipase C inhibitor, implying that Cav3-Gα q is responsible for this Ca 2+ activity. Taken together, these studies show that caveolae play a direct and active role in regulating basal Ca 2+ activity in cardiomyocytes. © 2011 by the Biophysical Society.

Golebiewska U.,State University of New York at Stony Brook | Golebiewska U.,Queensborough Community College | Zurawsky C.,State University of New York at Stony Brook | Scarlata S.,State University of New York at Stony Brook
Biochemistry | Year: 2014

γ-Synuclein is expressed at high levels in neuronal cells and in multiple invasive cancers. Like its family member α-synuclein, γ-synuclein is thought to be natively unfolded but does not readily form fibrils. The function of γ-synuclein is unknown, but we have found that it interacts strongly with the enzyme phospholipase Cβ (PLCβ), altering its interaction with G proteins. As a first step in determining its role, we have characterized its oligomerization using fluorescence homotransfer, photon-counting histogram analysis, and native gel electrophoresis. We found that when its expressed in Escherichia coli and purified, γ-synuclein appears monomeric on chromatographs under denaturing conditions, but under native conditions, it appears as oligomers of varying sizes. We followed the monomer-to-tetramer association by labeling the protein with fluorescein and following the concentration-dependent loss in fluorescence anisotropy resulting from fluorescence homotransfer. We also performed photon-counting histogram analysis at increasing concentrations of fluorescein-labeled γ-synuclein and found concentration-dependent oligomerization. Addition of PLCβ2, a strong γ-synuclein binding partner whose cellular expression is correlated with γ-synuclein, results in disruption of γ-synuclein oligomers. Similarly, its binding to lipid membranes promotes the monomer form. When we exogenously express γ-synuclein or microinject purified protein into cells, the protein appears monomeric. Our studies show that even though purified γ-synuclein form oligomers, when binding partners are present, as in cells, it dissociates to a monomer to bind these partners, which in turn may modify protein function and integrity. © 2013 American Chemical Society.

Danzi S.,Queensborough Community College | Klein I.,Private Practice
Medical Clinics of North America | Year: 2012

Thyroid hormone has profound effects on the heart and cardiovascular system. This article describes the cellular mechanisms by which thyroid hormone acts at the level of the cardiac myocyte and the vascular smooth muscle cell to alter phenotype and physiology. Because it is well established that thyroid hormone, specifically T 3, acts on almost every cell and organ in the body, studies on the regulation of thyroid hormone transport into cardiac and vascular tissue have added clinical significance. The characteristic changes in cardiovascular hemodynamics and metabolism that accompany thyroid disease states can then be best understood at the cellular level. © 2012 Elsevier Inc.

Meddles-Torres C.,Queensborough Community College | Hu S.,State University of New York at Stony Brook | Jurgens C.,State University of New York at Stony Brook
Journal of Infection and Public Health | Year: 2013

Background: Over 30% of the US population is colonized with methicillin resistant Staphylococcus aureus (MRSA). People within the community, without factors associated with Hospital Acquired (HA) MRSA, present with skin and soft tissue infections (SSTIs). Community Acquired MRSA (CA-MRSA) is resistant to antibiotics typically prescribed for SSTI. Many SSTIs are treated with antibiotics that are ineffective against drug resistant strains. Study objectives: This study examines the incidence of SSTIs associated with CA-MRSA, to determine if an increase in SSTI's is associated with changes in prescribing patterns for MRSA. Methods: A secondary analysis of the National Ambulatory Medical Care Survey (NAMCS) and National Hospital Ambulatory Medical Care Survey (NHAMCS) data was used to detect incidence of SSTIs based on ICD-9 coding between the periods of 1997-2002 and 2003-2008. Antibiotic prescribing patterns were examined for treatment. Results: Incidence of SSTIs increased by 84.7% from 1997-2002 to 2003-2008. Antibiotics prescribed for methicillin sensitive S. aureus decreased while treatment with MSRA antibiotics increased. Conclusion: There is an increased incidence of SSTI within the community, suggesting that CA-MRSA may be a contributing factor. Health care providers are recognizing the increased incidence of CAMRSA, and are treating SSTI with appropriate antibiotics. © 2013.

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