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Lahore, Pakistan

Khan I.U.,Government College University Lahore | Ashfaq M.,University of Gujrat | Razzaq S.N.,Government College University Lahore | Mariam I.,Queen Marry College
Journal of Liquid Chromatography and Related Technologies | Year: 2013

A fast, simple, specific, and accurate stability indicating liquid chromatographic method is described for simultaneous determination of piroxicam and paracetamol in bulk drugs and pharmaceutical formulations. Optimum chromatographic separations among the piroxicam, paracetamol, and stress-induced degradation products were achieved within 6 min by using a Hypersil BDS C8 column as stationary phase with acetonitrile and 0.02 M phosphate buffer pH 3.0 (60:40, v/v) as the mobile phase at a flow rate of 1.0 mL min-1 with detection using a diode array detector at 254 nm. ICH guidelines were used to validate the developed method. Linearity was from 1.6-6.4 g mL-1 for piroxicam and 26-104 g mL-1 for paracetamol. All the analytes including the degradation products were separated with acceptable peak tailing and resolution. The peak purity index for both the analytes after all types of stress is >0.999, indicating complete separation of both analytes peaks from the stress induced degradation products. The developed method can be successfully used for simultaneous determination of piroxicam and paracetamol in pharmaceutical formulations and stability studies. © 2013 Copyright Taylor and Francis Group, LLC. Source


Razzaq S.N.,Government College University Lahore | Khan I.U.,Government College University Lahore | Mariam I.,Queen Marry College | Razzaq S.S.,Medipharm Pharmaceuticals Kot Lakhpat
Chemistry Central Journal | Year: 2012

Background: A simple, specific, and fast stability indicating reverse phase liquid chromatographic method was established for instantaneous determination of moxifloxacin and prednisolone in bulk drugs and pharmaceutical formulations.Results: Optimum chromatographic separations among the moxifloxacin, prednisolone and stress-induced degradation products were achieved within 10 minutes by use of BDS Hypersil C8 column (250 X 4.6 mm, 5 μm) as stationary phase with mobile phase consisted of a mixture of phosphate buffer (18 mM) containing 0.1% (v/v) triethylamine, at pH 2.8 (adjusted with dilute phosphoric acid) and methanol (38:62 v/v) at a flow rate of 1.5 mL min -1. Detection was performed at 254 nm using diode array detector. The method was validated in accordance with ICH guidelines. Response was a linear function of concentrations over the range of 20-80 μg mL -1 for moxifloxacin (r2 ≥ 0.998) and 40-160 μg mL -1 for prednisolone (r2 ≥ 0.998). The method was resulted in good separation of both the analytes and degradation products with acceptable tailing and resolution. The peak purity index for both the analytes after all types of stress conditions was ≥ 0.9999 indicated a complete separation of both the analyte peaks from degradation products. The method can therefore, be regarded as stabilityindicating.Conclusions: The developed method can be applied successfully for simultaneous determination of moxifloxacin and prednisolone in pharmaceutical formulations and their stability studies. © 2012 Razzaq et al.; licensee Chemistry Central Ltd. Source


Razzaq S.N.,Government College University Lahore | Ashfaq M.,University of Gujrat | Khan I.U.,Government College University Lahore | Mariam I.,Queen Marry College
Journal of the Chilean Chemical Society | Year: 2012

The present study reports the development and validation of a simple, economic and sensitive HPLC method for the concurrent determination of naproxen and esomeprazole in pharmaceutical formulations. Isocratic chromatography was performed with C-18 column and mixture of phosphate buffer (pH 6.1) and acetonitrile in ratio of (40:60, v/v) at 1.5 mlmin-1. The eluents were monitored at 302 nm using UV detector. The method was isocratic in the range of 9.38 to 300 μgml-1 for naproxen and 0.5 to 16 μgml-1 for esomeprazole. Validation of the method was performed by testing parameters like linearity, accuracy, precision, robustness, specificity, LOD and LOQ values. In the specificity the drugs were subjected to forced degradation studies like acidic, basic, oxidative and thermal stresses. Both the analytes were separated within three minutes. As the method separates the degradation products produced during forced degradation studies from the active analytes so it can be used not only for regular determination of naproxen and esomeprazole but also for their stability studies. Source


Razzaq S.N.,Government College University Lahore | Khan I.U.,Government College University Lahore | Ashfaq M.,University of Gujrat | Mariam I.,Queen Marry College
Quimica Nova | Year: 2012

A simple, RP-HPLC method was established for determining moxifloxacin and ketorolac in pharmaceutical formulations. Moxifloxacin, ketorolac and their degradation products were separated using C8 column with methanol and phosphate buffer pH 3.0 (55:45 v/v) as the mobile phase. Detection was performed at 243 nm using a diode array detector. The method was validated using ICH guidelines and was linear in the range 20-140 ?g mL-1 for both analytes. Good separation of both the analytes and their degradation products was achieved using this method. The developed method can be applied successfully for the determination of moxifloxacin and ketorolac. Source


Razzaq S.N.,Government College University Lahore | Mariam I.,Queen Marry College | Khan I.U.,Government College University Lahore | Ashfaq M.,University of Gujrat
Journal of Liquid Chromatography and Related Technologies | Year: 2012

A fast, sensitive, and accurate stability indicating reverse phase high performance liquid chromatographic (RP-HPLC) method was developed and validated for simultaneous determination of gatifloxacin and ketorolac tromethamine in combined dosage form. Chromatographic separations were achieved on BDS Hypersil C 8 column (250 x 4.6 mm) with mobile phase that consisted of methanol and phosphate buffer (pH 3.0) in the ratio of (55:45 v/v) at a flow rate of 1.5 m Lmin -1. The analytes were detected at 270 nm using ultraviolet detection. The retention times of gatifloxacin and ketorolac tromethamine were found to be 2.460 and 6.366 min, respectively. When forced degradation studies were applied to both the drugs in combination, it was found that both gatifloxacin and ketorolac tromethamine were very stable under the basic, acidic, wet heat and oxidative environment. The method was linear in the concentration range of 30-90 μg mL -1for gatifloxacin and 50-110 μg mL -1 for ketorolac tromethamine. The correlation coefficients were found to be 0.9998 and 0.9999 for gatifloxacin and ketorolac tromethamine, respectively. The method resulted in good separation of both the analytes with acceptable tailing and resolution. The developed method can be used for routine determination of gatifloxacin and ketorolac tromethamine in commercial formulations. Copyright © Taylor & Francis Group, LLC. Source

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