Qinhuangdao Entry Exit Inspection and Quarantine Bureau

Qinhuangdao, China

Qinhuangdao Entry Exit Inspection and Quarantine Bureau

Qinhuangdao, China
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Wang R.,China Agricultural University | Chen S.,Key Laboratory of Functional Dairy | Pang R.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Guo H.,Beijing Laboratory for Food Quality and Safety | And 2 more authors.
Journal of Chinese Institute of Food Science and Technology | Year: 2016

During the public holiday, intestinal disorders phenomenon such as diarrhea or constipation caused by surfeit and diet imbalance is very common in China. Probiotic could modulate intestinal microflora and improve gut health. In this study, a parallel control human trial was performed to evaluate the effect of Lactobacillus casei L9 on human intestinal microflora and intestinal health during the public holiday. A total of 40 healthy adults were assigned to two groups randomly. There were 20 people in the control group, who continued their normal diet with exclusion of dairy fermented products. Another 20 subjects were in treatment group, who successfully complete the one week baseline period entered ingestion period. During ingestion period, subjects drank one bottle (100 mL) of the study product (vegetable protein drink containing 1.0x108 CFU/mL L9) for two weeks. Fecal samples were collected once every week. The change of intestinal microflora were analyzed by real-time PCR. The change in stool consistency was recorded in subject daily. The results showed that BS (Bristol Stool Form Scale) score of treatment group was significantly higher than the score of control group(P<0.05), which indicated that L9 could increase the softness of stool, and improve constipation. In following-up period, the control group had higher number of potentially pathogenic Enterococcus genus and Escherichia coli (P <0.05) and lower number of beneficial Bifidobaceterium (P<0.05), compared with baseline period. That indicated intestinal microflora changed significantly during holiday. Compared to control group and baseline period, significant lower in potentially pathogenic Escherichia coli (P<0.01) and increase in beneficial Lactobacillus (P<0.01) were abserved in treatment group in ingestion period. Compared to following-up period, treatment group had higher number of Bifidobaceterium in ingestion period (P<0.05). These results indicated that Lactobacillus casei L9 could modulate intestional microflora and improve gut health of human during the public holiday. © 2016, Editorial Office of Journal of CIFST. All right reserved.


Pang G.-F.,Chinese Academy of Inspection and Quarantine | Fan C.-L.,Chinese Academy of Inspection and Quarantine | Zhang F.,Chinese Academy of Inspection and Quarantine | Li Y.,Chinese Academy of Inspection and Quarantine | And 7 more authors.
Journal of AOAC International | Year: 2011

An efficient and sensitive method has been established for simultaneous determination of 653 pesticides in teas by GC/MS and HPLC/MS/MS. The method involved extraction with acetonitrile followed by cleanup using Cleanert-TPT SPE and subsequent identification and quantitation of 490 pesticides by GC/MS and 448 pesticides by HPLC/MS/ MS. The LODs for pesticides determined by GC/MS were between 1.0 and 500 μg/kg, and those determined by HPLC/MS/MS were between 0.03 and 4820 μg/kg. At the low fortification levels of 0.01-100 μg/kg, the average recoveries of 94% of the pesticides determined by GC/MS were between 60 and 120%, 77% of which had an RSD below 20%. For 91% of pesticides determined by HPLC/MS/MS, the average recoveries were between 60 and 120%, 76% of which had an RSD below 20%. The paper also reports a novel SPE column, Cleanert TPT, which comprised graphitized carbon black (PestiCarb), polyamine silica, and amide polystyrene for purifying the tea samples. The results indicated good repeatiblity and reproducibility. © 2012 Publishing Technology.


Yu L.,Hebei University | Cao Y.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Zhang J.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Cui Z.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Sun H.,Hebei University
Food Additives and Contaminants - Part A Chemistry, Analysis, Control, Exposure and Risk Assessment | Year: 2012

Medicinal herbs have a very important role in health protection and disease control, and have been used in health foods. Polycyclic aromatic hydrocarbons (PAHs) have carcinogenic, biological and mutagenic effects. In this paper, the content of 16 PAHs as representative contaminants in nine Chinese medicinal herbs, as additives for health foods, was investigated in order to ensure food safety from this source. A highly sensitive isotope dilution-gas chromatography-tandem mass spectrometry (ID-GC-MS/MS) method combined with gel permeation chromatography (GPC) and solid-phase extraction (SPE) was developed. Calibration curves showed good linearity for all PAHs (R2> 0.999), and the limit of quantification (LOQ) ranged from 0.42 to 2.7 μg kg-1. Average recoveries for these compounds were in the range of 52.5-117%, 52.6-119% and 81.4-108% at the concentrations of 10, 50 and 250 μg kg-1 with RSD of 1.8-15%, 0.9-15% and 1.0-15%, respectively. The proposed method was used for the analysis of nine Chinese medicinal herbs. Total levels of PAHs varied from 98.2 μg kg-1 (cassia seed) to 2245 μg kg-1 (eucommia bark). The highest level was found for phenanthrene (Phe) in liquorice root (631.3 μg kg-1), indigowoad leaf (551.0 μg kg-1), rose flower (435.2 μg kg-1) and eucommia bark (432.3 μg kg-1). The proposed method could provide a useful basis for safety monitoring of herbs and risk management for PAHs in the health food industry. © 2012 Copyright Taylor and Francis Group, LLC.


Wang S.,Yanshan University | Di N.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Song J.,Yanshan University
Advanced Materials Research | Year: 2013

Increasing use of pyrethroid insecticides in tidal farms has resulted in the severe pollution of coastal water environment and sedimentary environment. In this work, the migration and transformation of three pyrethroid insecticides, fenpropathrin, cypermethrin and deltamethrin in Bohai coastal zone were studied. The results showed that the adsorption of pyrethroids on the coastal sediments matched pseudo-second-order kinetics and the chemical adsorption played a major role. The degradation of three pyrethroids included chemical degradation and biological degradation. The chemical degradation had predominance function at the initial 14 hours, but the biological degradation effect was increasing continuously in the transformation process, so that the proportion of biological degradation in total degradation reached about 68% at 110h. M-phenoxyphenyl-acetonitril was identified as the stable degradation product by GC-MS. © (2013) Trans Tech Publications, Switzerland.


Ding S.,Northeastern University China | Zhao X.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau
2011 IEEE 3rd International Conference on Communication Software and Networks, ICCSN 2011 | Year: 2011

The key problem in P2P network is effectively to locate resources. Chord has been widely used as a routing protocol in structured P2P networks, but there is a lot of redundant information in the node's finger table. By analyzing Chord protocol, an improved Chord algorithm is proposed in this paper. Analysis on theory and simulation results show that the improved finger tables reduce redundancy and improved Chord algorithm has better performance. © 2011 IEEE.


Cui Z.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Qian Y.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Ge N.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Zhang J.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Analytical Methods | Year: 2014

A robust method for the determination of six organotin compounds (OTs), monobutyltin (MBT), dibutyltin (DBT), tributyltin (TBT), monophenyltin (MPhT), diphenyltin (DPhT) and triphenyltin (TPhT), in sea products was developed using gas chromatography-triple quadrupole tandem mass spectrometry (GC-MS/MS). The target compounds were extracted by hexane containing 0.01% tropolone, derivatizated by Grignard reagent n-PrMgBr, purified on a serial connection of silica and florisil solid-phase extraction (SPE) columns, and finally analyzed by GC-MS/MS. Improved sensitivity and selectivity were achieved using tandem MS/MS rather than single MS, especially in reducing complex interferences in biotic matrices. The limits of detection (LODs) for the six OTs were all below 0.1 μg Sn per kg in the case of wet samples, and were no higher than 0.5 μg Sn per kg for dry samples. The linearity coefficients (r2) for the six OTs were all above 0.999 within the linear range from 0.4 to 200 μg Sn per kg. The accuracy of the method was thoroughly validated using the certified reference material Mytilus edulis (ERM-CE477) and a spiked recovery test in four different biotic matrices, including tonguefishes, sea snails (Neverita didyma), scallops (Patinopecten yessoensis) and the Asian moon scallop. The butyltin concentrations determined for CE477 agreed well with the certified values, and the relative standard deviations (RSDs) for the six OTs were all below 12.1%. The spiked recoveries in four biotic matrices were within the range 70.5-105.3% for MBT, DBT, TBT, DPhT and TPhT, and 82.2-133.5% for MPhT, and the RSDs ranged from 0.5% to 12.5%. The proposed methodology was applied to the determination of butyltin and phenyltin compounds in nine different sea products sampled from the Bohai coast, China, with total OTs ranging from 1.36 to 20.54 μg Sn per kg wet weight. © 2014 The Royal Society of Chemistry.


Li Y.,Metrohm China Ltd Company | Yu H.,Metrohm China Ltd Company | Wang F.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau
Chinese Journal of Chromatography (Se Pu) | Year: 2016

An ion chromatographic method was developed for the determination of perchlorate in wine. The content of perchlorate in four kinds of wine was determined. Metrosep A Supp5 column (150 mm×4.0 mm) was used for the separation with the eluent of 1.0 mmol/L Na2CO3-acetone (85: 15, v/v) at the flow rate of 0.8 mL/min, and the column temperature was 40°. Perchlorate had good linearity in the range of 0.1-10.0 mg/L. The recoveries of the method were more than 86.0%, and the relative standard deviations were less than 2.6%. The method is simple and rapid, and can be used for the determination of perchlorate in wine.


Lian Y.-J.,Shandong Agricultural University | Pang G.-F.,Chinese Academy of Inspection and Quarantine | Shu H.-R.,Shandong Agricultural University | Fan C.-L.,Chinese Academy of Inspection and Quarantine | And 4 more authors.
Journal of Agricultural and Food Chemistry | Year: 2010

The article demonstrates a method of simultaneous determination for 352 pesticide residues in grapes using primary-secondary amine (PSA) matrix solid phase dispersion (MSPD) cleanup and gas chromatography-mass spectrometry- selected ion monitoring (GC-MS-SIM). Grape samples (15 g) were mixed with 6 g of anhydrous magnesium sulfate and 1.5 g of sodium chloride, and then extracted with acetonitrile (15 mL) and cleaned up with 0.3 g of dispersive PSA. The analytes were determined by GC-MS-SIM. Four injections for one sample were acquired to cover a total of 352 pesticides. The limit of detection (LOD) for the method was 0.0017-0.2667 mg kg-1, depending on the nature of compounds. The linear correlation coefficient (r) was equal to or greater than 0.95; at low, medium, and high fortification levels, recoveries ranged from 45% to 136% for 352 pesticides, among which the recoveries between 60%-120% accounted for 97%. The pesticides for which the relative standard deviations (RSD) were equal to or below 20% accounted for 95%. A positive of nine varieties of grape samples was detected out, one of which was abtained Changli city, Hebei province, China. Pesticides were identified by the retention time, molecule ions, fragment ions, and the abundance ratio of the selected ions. The analytical method was rugged, quick, cheap and effective, and suitable for the determination of a wide scope of 346 pesticides in grapes. © 2010 American Chemical Society.


Liu Y.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Ge N.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Wang F.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | Li J.,Qinhuangdao Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2012

A method was developed for the simultaneous determination of 57 residual volatile organic solvents (including several alkanes, aromatic hydrocarbons, alcohols, ketones, esters and ethers) in honey by headspace gas chromatography-mass spectrometry (HS-GC/ MS). The honey sample was dissolved with water in a headspace vial, and the equilibration of the sample in the headspace vessel was achieved at 80°C in 30min. A DB-624 capillary chromatographic column (60 m ×0.25mm × 1.40μm) was used for the separation of 57 volatile organic solvents, and the analysis was performed by GC/MS. The external calibrations were used for the quantification. The linear ranges of the method were 0.005-0.2μg for the alkanes, aromatic hydrocarbons and ethers, 0.05 -2.0μg for the esters, 0.5-20μg for the ketones, 2.5-100μg for the alcohols. The correlation coefficients were more than 0.996 for all the volatile organic solvents. The recoveries and the relative standard deviations were from 61.0% to 113.1% and 1.9% to 9.8%, respectively, at the spiked levels of 1.0-20μg/kg for the alkanes, aromatic hydrocarbons and ethers, 10-200μg/kg for the esters, 100-2 000μg/kg for the ketones, 500-10 000μg/kg for the alcohols. The limits of detection were 1.0μg/kg for the alkanes, aromatic hydrocarbons and ethers, 10μg/kg for the esters, 100μg/kg for the ketones, 500μg/kg for the alcohols. The method is simple, rapid, sensitive and accurate, and can be used for the simultaneous determination of residual volatile organic solvents in honey samples.


PubMed | Hebei University of Technology and Qinhuangdao Entry Exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Journal of dairy science | Year: 2015

A simple and rapid multi-class multi-residue analytical method was developed for the screening and quantification of veterinary drugs in milk by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). A total of 90 veterinary drugs investigated belonged to almost 20 classes including lincomycins, macrolides, sulfonamides, quinolones, tetracyclines, -agonists, -lactams, sedatives, -receptor antagonists, sex hormones, glucocorticoids, nitroimidazoles, benzimidazoles, nitrofurans, and some others. A modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) procedure was developed for the sample preparation without the solid-phase extraction step. The linearity, sensitivity, accuracy, repeatability, and reproducibility of the method were fully validated. The response of the detector was linear for each target compound in a wide concentration range with a correlation coefficient (R(2)) of 0.9973 to 0.9999 (among them R(2)>0.999 for 73 of 90 analytes). The range of the limit of quantification for these compounds in the milk ranged from 0.10 to 17.30g/kg. The repeatability and reproducibility were in the range of 2.11 to 9.62% and 2.76 to 13.9%, respectively. The average recoveries ranged from 72.62 to 122.2% with the RSD (n=6) of 1.30 to 9.61% at 3 concentration levels. For the screening method, the data of the precursor and product ions of the target analytes were simultaneously acquired under the all ions MS/MS mode in a single run. An accurate mass database for the confirmation and identification of the target compounds was established. The applicability of the screening method was verified by applying to real milk samples. The proposed analytical method allows the identification and confirmation of the target veterinary drugs at trace levels employing quick analysis time. Certain veterinary drugs were detected in some cases.

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