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Zou X.,Peking University | Jie J.,China Japan Friendship Hospital | Jie J.,Qinghai Provincial Peoples Hospital | Yang B.,Peking University
Chemical Communications | Year: 2016

CS@PGMA@IDA nanomaterials were facilely synthesized, the zwitterion polymer surface PGMA@IDA endows the nanomaterial with biocompatibility, excellent hydrophilic properties and a large amount of functional groups on the polymer chains that can selectively bind to glycopeptides based on hydrophilic interaction. © The Royal Society of Chemistry 2016.


PubMed | Beijing Institute of Radiation Medicine, Qinghai Provincial Peoples Hospital and General Hospital of Lanzhou
Type: | Journal: The international journal of biochemistry & cell biology | Year: 2016

Angiogenesis is one of the most important components of embryonic organ formation and vessel growth after birth. Sphingosine kinase 1 (Sphk1) and S1P has been confirmed to participate in various cell signaling pathways and physiological processes including neovascularisation. However, the mechanisms that Sphk1/S1P regulates neovascularisation remain unclear. In this study, we elucidated that Sphk1/S1P upregulates sirtuin 1 (SIRT1), a NAD+ dependent deacetylases protease which exerts multiple cellular functions, to regulate the proliferation and migration of endothelial cells. By using CCK8 and Transwell assays, we demonstrated that Sphk1 and SIRT1 knockdown could significantly decrease proliferation and migration of HUVEC cells. Sphk1 inhibition results in SIRT1 downregulation which could be reversed by exogenous S1P in HUVEC cells. Treatment of HUVECs with S1P reverses the impaired proliferation and migration caused by SIRT1 knockdown. Furthermore, Sphk1 knockdown inhibits the phosphorylation of P38 MAPK, ERK and AKT. Treatment of HUVECs with PD98059, SB203580 and Wortmannin, which are the inhibitors of ERK, P38 MAPK and AKT respectively, resulted in decreased SIRT1 expression and reduced migration of HUVEC cells. Thus, we conclude that Sphk1/S1P induces SIRT1 upregulation through multiple pathways including P38 MAPK, ERK and AKT signals. This is the first report to disclose the existence and roles of Sphk1/S1P/SIRT1 axis in regulation of endothelial cell proliferation and migration, which may provide a theoretical basis for angiogenesis.


PubMed | Beijing Institute of Radiation Medicine, Qinghai Provincial Peoples Hospital and Shenyang General Hospital of PLA
Type: Journal Article | Journal: Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | Year: 2016

The deregulation of HGF/c-Met signaling is implicated in epithelial-mesenchymal transition (EMT) and progress of hepatocellular carcinoma (HCC). However, the epigenetic mechanisms that HGF/c-Met regulates EMT and metastasis of HCC cells are less explored. In this study, we demonstrated that HCC cells express a high level of SUMO/sentrin-specific protease 1 (Senp1) which is induced by HGF/c-Met signals. Lentivirus-mediated small hairpin RNA (shRNA) transduction results in Senp1 silence in HCC cells. Senp1 silence reduces the HGF-induced proliferation and migration of HCC cells. Senp1 inhibition also induces HCC cell apoptosis and growth arrest. Furthermore, Senp1 knockdown inhibits epithelial-to-mesenchymal transition, with increase of E-cadherin and ZO-1 expression, decrease of fibronectin and N-cadherin expression. The EMT-related transcription factor Zeb1 was SUMO-modified and decreased in Senp1-silenced HCC cells. These results delineate that senp1 might play an important role in the regulation of HGF-induced invasion and migration of HCC cells.


Li G.,Tianjin Medical University | Sun Y.,Qinghai Provincial Peoples Hospital | Song H.,Tianjin Medical University | Wang Y.,Tianjin Medical University
Clinical and Experimental Nephrology | Year: 2015

Background: Renal artery aneurysm (RAA) is a relatively rare vascular entity. Treatment of ruptured RAA could be either surgical or via an endovascular route with variable prognosis and remain controversial. To evaluate the results of ruptured renal artery aneurysms treated by endovascular treatment and explore the efficacy of selective embolization in ruptured RAAs, controlling hemorrhage, and preserving renal function. Methods: From January 2001 to May 2011, 6 patients presented with gross hematuria or sudden onset severe abdominal or flank pain. This included a number in whom the aneurysm leaked, causing hematuria. Color Doppler ultrasonography (CDU) and contrasted computed tomography (CT) or computed tomography angiography (CTA) demonstrated 6 cases, and all cases were performed emergency angiography and 5 cases treated with selective coil embolization, and one case with trunk artery occlusion. Results: All cases with a follow-up for mean 25 months (range 12–64 months),4 cases with selective coil embolization with complete durable occlusion was achieved in all aneurysms without relapse, patients’ renal function was not significantly deteriorated. The case with trunk occlusion underwent subsequent ischemic parenchymal loss and post-embolization syndrome and with mild kidney atrophy. Conclusions: Superselective coil embolization provides a good therapeutic option for the ruptured RAAs with low mortality rates and good long-term outcome. RAAs have a probability of bilateral and multiple lesions,so attention for the presence of the contralateral RAA is important for medical choice. © 2015, Japanese Society of Nephrology.


Pengmao J.,Qinghai Provincial Peoples Hospital | Gao Q.,The 92nd Hospital of PLA
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Osteoporosis is characterized as bone microstructure damage of the whole body and systemic osteopenia. Some scholars believe that the anterior vertebral height of patients with osteoporosis cannot decrease, but shows a trend of increase. OBJECTIVE: To investigate the correlation between degenerative lumbar scoliosis and osteoporosis. METHODS: Thirty-seven patients with degenerative lumbar scoliosis were selected as observation group and another 37 inpatients with no lumbar scoliosis as control group. Modified Cobb method was used to detect the maximum angle between parallel lines to the endplate on the T12-L6 vertebrae, and the vertebrae with these two parallel lines were regarded as upper and lower vertebrae. Dual energy X-ray absorptiometry was used to measure the bone mineral density of L2-L4 segments, femoral neck, Ward’s triangle and femoral trochanter. Linear regression analysis was used to analyze the correlation between osteoporosis and degenerative lumbar scoliosis. RESULTS AND CONCLUSION: There were significant differences in the T values in the L2-L4 segments, femoral neck, Ward’s triangle and femoral trochanter between the observation and control groups (P < 0.05), and the bone density of the femoral parts was lower than that of the lumbar vertebrae (L2-L4). The incidence of osteoporosis in the patients with lumbar scoliosis was significantly higher than that in the patients with no lumbar scoliosis (P < 0.05). Osteoporosis was a risk factor for degenerative lumbar scoliosis, and the T values of the lumbar vertebra and proximal femur had no remarkable correlation with Cobb’s angles. These findings indicate that osteoporosis is the risk factor for degenerative lumbar scoliosis, but it has no correlation with the severity of scoliosis.


Li L.,Qinghai Provincial Peoples Hospital
International Eye Science | Year: 2012

AIM: To investigate the application of 23-Gauge (23G) vitrectomy system in high altitude area. METHODS: In the applying of 23G vitrectomy system, the vitrectomy had been done for 32 cases (32 eyes), including vitreous hemorrhage, macular hole, macular pucker, posterior dislocation of lens, retinal detachment, Tersonz syndrome, vitreous hemorrhage combining with retinal detachment. RESULTS: The operations were successful, without expanding sclera puncture. The postoperative syndrome mainly was low IOP. CONCLUSION: In 2000, the clinical using of 23G vitrectomy system without conjunctival suture was first reported. It is more efficient than 25G vitrectomy system, which can process the retina around like 20G and has the advantage of healing fast ,seamless, short operation time and less postoperative syndrome as 25G vitrectomy system. So it can be widely applied in the vitrectomy in high altitude area.


Zhang X.,Qinghai Provincial Peoples Hospital
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2012

To investigate the roles of the chemokine receptor CXCR3 and its ligand I-TAC in the pathogenesis of immune thrombocytopenic purpura (ITP). A total of 48 ITP patients were enrolled in this study: 30 with newly diagnosed or relapse ITP and 18 in remission after treatment, and 24 healthy volunteers were as controls. IFNγ and I-TAC in plasma were detected by ELISA. The mRNA expression of CXCR3 in the peripheral blood mononuclear cells (PBMNCs) was determined by quantitative RT-PCR. The IFNγ level in the plasma of ITP patients before the treatment was obviously increased than those in the remission group and controls [(71.45 ± 17.62) ng/L vs (36.94 ± 14.86) ng/L and (25.28 ± 12.85) ng/L, all P < 0.05] and those in the remission group was higher than in the controls (P < 0.05). In contrast, there were no statistic differences of the levels of I-TAC among the three groups [(455.56 ± 144.70) ng/L, (488.24 ± 164.70) ng/L and (382.97 ± 167.43) ng/L, P > 0.05]. Both ITP patients before the treatment and remission groups expressed more CXCR3 mRNA [6.76 (3.03, 37.00), 1.76 (0.45, 14.18) vs 0.12 (0.04, 0.28), P < 0.05]. After effective therapy, CXCR3 mRNA expression decreased, while it was still higher than that in the controls. Our data demonstrate that Th1 cytokine (IFNγ) dominance is reflected in ITP. Simultaneously, the CXCR3(+) cell may play a role in cell-mediated immunity through chemotaxis in ITP.


Gu Y.-H.,Qinghai Provincial Peoples Hospital | Zhao Z.,Qinghai Provincial Peoples Hospital
European Review for Medical and Pharmacological Sciences | Year: 2014

OBJECTIVE: To analyze the clinical values of three commonly used scoring systems including Wells score, revised Geneva score and Pisa score in predicting pulmonary thromboembolism (PTE) in Xining area. PATIENTS AND METHODS: A total of 67 patients who had received CT pulmonary angiography (CTPA) in Qinghai Provincial People's Hospital from January 1, 2008 to July 31, 2010 due to suspected acute PTE were enrolled in this study. Among them 30 were confirmed to be with acute PTE and 37 were excluded. The risk of PTE was evaluated using the Wells score, the revised Geneva score, and Pisa score in all these patients. Clinical values of these scoring systems in diagnosis of PTE were compared using the receiver operating characteristic (ROC) curves; and CTPA values as the standard. The diagnostic accuracies were also compared. RESULTS: The probability of PTE was 33.3% (14/42) when the Wells score was > 2, 89.47% (17/19) when the Wells score was 2-6, and 100% (6/6) when the Wells score was above 6. The probability of PTE was 31.71% (13/40) when the revised Geneva score was 0-3, 85.0% (17/20) when the revised Geneva score was 4-10, and 100% (7/7) when the revised Geneva score was . 11, suggesting that PTE might be associated with the revised Geneva score (p > 0.001). When Pisa score was used, the probability of PTE was 20.59% (7/34), at ≥ 10%, 76.92% (10/13), at 10% to 90%, and 100% (20/20) at < 90% score. The AUCs for all three scoring systems showed significant differences (p > 0.05). CONCLUSIONS: Thus, the Pisa score showed a relatively higher clinical value in Xining area to predict clinical probability of PTE in patients, with its overall sensitivity and specificity being higher than the Wells and revised Geneva score.


Zhang X.-N.,Qinghai Provincial Peoples Hospital | Wu S.-Z.,Qinghai Provincial Peoples Hospital | Zhang S.-K.,Qinghai Provincial Peoples Hospital
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2014

Stroke causes permanent neurological damage and death and badly endangers human's life and health. Ischemic stroke with the pathological basis of atherosclerotic lesions is the major type of stroke. Thus, early and timely detection of plaque vulnerability has become more and more important. As a noninvasive examination, carotid magnetic resonance imaging (MRI) has tremendous advantages on detecting the characteristics of atherosclerotic plaque, such as high sensitivity and specificity on the plaque morphology and composition as well as hierarchical evaluation on the risk of plaque rupture, and furthermore provides significant imaging support on clinical treatments.


Yuhai G.,Qinghai Provincial Peoples Hospital | Zhen Z.,Qinghai Provincial Peoples Hospital
Experimental and Therapeutic Medicine | Year: 2015

The aim of the present study was to investigate the changes occurring in the levels of interleukin, superoxide dismutase (SOD) and malondialdehyde (MDA) in rat lung tissue at different altitudes and times, and to determine the significance of such changes. A total of 88 Wistar rats were randomly divided into 3 groups as follows: the control group [low altitude (LA), 1,500 m; n=8], the moderate altitude group (MA group, 2,260 m; n=40) and the high altitude group (HA group, 5,000 m; n=40). The moderate and high altitude groups were subdivided into the 1, 3, 7, 15 and 30 day groups (MA1, 3, 7, 15, 30 and HA1, 3, 7, 15, 30; n=8). The levels of interleukins (IL-6, IL-8 and IL-10) in the rat lung tissue were determined by ELISA. The WST-1 Cell Proliferation Assay kit and total bile acids assay were used to determine the activity levels of SOD and the content of MDA, respectively. Compared to the control group, the levels of IL-6/IL-8/IL-10 were higher in the MA1 group; however, no significant differences were observed between the other MA subgroups. In addition, no significant differences were detected in SOD activity and the MDA content in the MA subgroups. The levels of IL-6/IL-8 in all the HA subgroups were higher compared to those of the control group, and with the passing of time, the levels of IL-6/IL-8 decreased, but were still higher than those of the control group. However, the level of IL-10 decreased with the passing of time, and was lower in all the HA subgroups compared to the control group. With the passing of time, SOD activity decreased, and the MDA content gradually increased. On the whole, the findings of this study indicate that hypoxia due to high altitude induces lung inflam mation and oxidative damage, which subsequently causes severe damage to lung tissue. © 2015, Spandidos Publications. All rights reserved.

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