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Wang J.,Harbin Medical University | Wang J.,Heilongjiang University of Chinese Medicine | Wang A.,Qinghai Province Peoples Hospital | Zeng H.,Hunan Wangwang Hospital | And 4 more authors.
Anatomical Record | Year: 2012

Triptolide (TP) has been used in the treatment of rheumatoid arthritis (RA), but its mechanism of action is not understood. T-cell activation and associated release of cytokines appear to be major factors in the pathogenesis of RA. The overexpression of T-cell receptor (TCR) variable gene (V gene) fragments can cause the activation and infiltration of autoreactive T cells. This study examines the effects of TP on rats with collagen-induced arthritis (CIA). The levels of interleukin-10 (IL-10) in the serum were examined with ELISA. Compared to the CIA group, the levels of IL-10 were greater in the TP treatment group. Real-time quantitative polymerase chain reaction confirmed that the expression of TCR V beta (BV) 15 and TCR BV19 was increased in the CIA group, whereas in the TP treatment group, the expression was decreased. In this study, TP was found to enhance IL-10 levels and decrease the expression levels of TCR BV15 and TCR BV19. These changes might help explain the effectiveness of TP in the treatment of RA. © 2012 Wiley Periodicals, Inc. Source

Zhu Y.-B.,Beijing University of Chinese Medicine | Wang Q.,Beijing University of Chinese Medicine | Cai J.,Geriatrics Institute of Integrated Traditional Chinese and Western Medicine | Song X.-H.,Qinghai Province Peoples Hospital | Yan X.,Beijing University of Chinese Medicine
Journal of Chinese Integrative Medicine | Year: 2010

Objective: To investigate the relationships between constitutional types of traditional Chinese medicine (TCM) and hypertension so as to provide epidemiological evidence for the theory of correlation between constitution and disease. Methods: A cross-sectional survey of TCM constitution data from a population of 7 782 from Beijng and 8 provinces of China (Jiangsu, Anhui, Gansu, Qinghai, Fujian, Jilin, Jiangxi, and Henan) was made in the study. The survey of TCM constitutions was performed by standardized TCM Constitution Questionnaire. Discriminatory analysis was used to judge the individual constitutional types including normal constitution, and qi deficiency, yang deficiency, yin deficiency, phlegm-dampness, damp-heat, blood stasis, qi stagnation and special constitutions. A multiple stepwise logistic regression analysis was applied to explore the significantly influential constitutional factors of hypertension. Results: After controlling several factors like gender, age, marital status, occupation, and educational background, three TCM constitutional factors according to different degrees of relative risks were entered into the multiple stepwise logistic regression model. The three factors were phlegm-dampness, yin deficiency and qi deficiency constitutions, and the odds ratio (OR) and 95% confidence interval (CI) were 2.00 [1.58, 2.55], 1.66 [1.33, 2.08] and 1.37 [1.13, 1.66] respectively. The main constitutional influential factors of hypertension in male patients were phlegm-dampness and yin deficiency constitutions, with OR and 95% CI of 1.61 [1.22, 2.14] and 1.60 [1.17, 2.19]. Phlegm-dampness, yin deficiency and qi deficiency constitutions were the main constitutional influential factors of hypertension in female patients. The OR and 95% CI were 2.80 [1.79, 4.39], 1.55 [1.13, 2.14] and 1.39 [1.05, 1.84] respectively. Phlegm-dampness constitution had more influence on hypertension in female patients than other constitution types. Conclusion: Phlegm-dampness, yin deficiency, and qi deficiency constitutions are the main influential factors of hypertension. Hypertensive patients with different gender have different constitutional influential factors. Source

Wang X.-S.,Qinghai Province Peoples Hospital | Zhang G.-X.,Qinghai Province Peoples Hospital | Dong X.-Q.,Soochow University of China | Yang J.-C.,Soochow University of China | Sheng W.-H.,Soochow University of China
Chinese Journal of Cancer Prevention and Treatment | Year: 2014

OBJECTIVE: To explore the anti-cancer effect and chemotherapy sensitization of adenovirus-mediated ING4 gene on the human breast cancer multidrug resistant cell line MCF-7/ADR and its possible mechanisms in vitro.METHODS: The human breast cancer multidrug resistant cells MCF-7/ADR were infected by Ad-ING4 combined with doxorubicin (ADR) in vitro and were randomly divided into five groups: the PBS negative control group (PBS group), the idling adenovirus group (Ad-GFP group), the adenovirus-mediated ING4 group (Ad-ING4 group), the ADR positive control group with the final concentration 3.0 μg/mL (ADR group), the group of Ad-ING4 combined with ADR final concentration 3.0 μg/mL (referred to as the joint group).The methods of reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of ING4 in breast cancer MCF-7/ADR cells. The influence of Ad-ING4 transfection on cell proliferation was evaluated by CCK-8 assay, The cell cycle of MCF-7/ADR cells infected with Ad-ING4 and doxorubicin was detected by flow cytometry(FCM). The influence of Ad-ING4 and doxorubicin on MCF-7/ADR cell apoptosis was detected by double fluorescent labeling with Annexin V-PE/7-AAD. The expression of apoptosis-related genes (Bax, Bcl-2, Survivin) was detected by RT-PCR and Western blot.RESULTS: The adenovirus-mediated ING4 was successfully transcribed in MCF-7/ADR cells. The adenovirus-mediated ING4 and doxorubicin significantly inhibited MCF-7/ADR cell growth, induced cell apoptosis and made the cell cycle partly arrested in G2/M phase(F=129.294, P<0.05). The Ad-ING4 combined with Doxorubicin could acquire higher rate of growth inhibition and apoptosis. After cultured for four days, the growth inhibition rate of the joint group was significantly higher than Ad-ING4 group and ADR group, (73.13±3.78)% vs (19.29±1.53)% and (48.39±2.96)%, F=257.397, P<0.05). The apoptosis rate of the joint group was significantly higher than that of Ad-ING4 group and ADR group, (26.48±3.13)% vs (11.57±1.26)% and (17.21±2.34)%, F=30.253, P=0.001. Ad-ING4 and ADR not only increased the expression of Bax, but also lowered the expression of Bcl-2 and Survivin. Compared with other groups, the joint group's effect was the most remarkable(P<0.05).CONCLUSIONS: Ad-ING4 can inhibit the growth of MCF-7/ADR cells and induce apoptosis in vitro. ING4 can increase doxorubicin chemotherapeutic effect. Its mechanism may be related to the ratio of Bcl-2/Bax reduced by ING4 and the reduced expression level of Survivin. ©, 2014, The Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved. Source

Li Y.,Peking Union Medical College | Jing L.,Qinghai Province Peoples Hospital | Jiang J.,Tongji University | Wang Z.,Xuzhou Medical College | And 14 more authors.
Pharmacoepidemiology and Drug Safety | Year: 2014

Purpose: The aim of this study was to assess the efficacy and safety of ivabradine (Iva) noninferiority to atenolol (Aten) in Chinese patients with chronic stable angina pectoris. Methods: In this double-blind, double-dummy trial, patients with symptomatic angina pectoris and positive exercise tolerance test were randomized into the Iva [5 or 7.5mg bis in die (BID)] or Aten group (12.5 or 25mg BID) according to computer-generated random numbers for 12weeks. Results: One hundred and sixty-eight patients were randomized to the Iva group and 166 to the Aten group. In a full analysis set, increases in the total exercise duration (TED) were 54.3±120.1seconds with Iva 5mg and 58.8±114.7seconds with Aten 12.5mg at the fourth week, and at the 12th week, TED improved by 84.1±130.5seconds with Iva and 77.8±126.6seconds with Aten (95%CI: -21.4-34.1seconds, p=0.0011 for noninferiority). The analysis of per protocol set yielded similar results (95%CI: -31.4-33.0seconds, p=0.0131 for noninferiority). Heart rate was reduced in both groups at rest and during peak exercise. There were small, nonsignificant differences in the number of adverse events between the two groups (66 in Iva and 73 in Aten, p>0.05). Nine patients (5.42%) were reported to develop phosphenes/luminous phenomena and blurred vision in the Iva group (p=0.0035). Conclusions: Iva is effective in reducing heart rates and improving exercise capacity and noninferior to Aten in Chinese patients with chronic stable angina pectoris. Iva is well tolerated and safe. © 2014 John Wiley & Sons, Ltd. Source

Xu G.-C.,Qinghai Province Peoples Hospital | Zan M.,Qinghai Province Peoples Hospital
World Chinese Journal of Digestology | Year: 2015

AIM: To assess the curative efficacy of Shenqi Fuzheng injection in combination with Endostar in the treatment of patients with advanced gastric cancer (AGC) as well as the effect on the immune function and quality of life. METHODS: One hundred and fifty AGC patients were randomly and equally divided into two groups: a control group and an observation group. The control group was treated with Endostar in combination with FOLFOX regimen, and the observation group was treated with Shenqi Fuzheng injection on the basis of treatment in the control group. The curative efficacy, toxic side effects, immune function and quality of life were compared for the two groups. RESULTS: The curative efficacy between the observation group and the control group had no significant difference (62.7% vs 56.0%, P > 0.05). Serum levels of tumor supplied group of factor (TSGF), carbohydrate antigen 72-4 (CA72-4) and carcino-embryonic antigen (CEA) in the observation group were significantly lower than those in the control group after treatment (49.37 U/mL ± 3.24 U/mL vs 54.46 U/mL ± 4.33 U/mL, 3.27 U/mL ± 2.36 U/mL vs 8.34 U/mL ± 2.12 U/mL, 0.41 ng/mL ± 0.24 ng/mL vs 0.48 ng/mL ± 0.17 ng/mL, P < 005). Compared to the control group, the incidences of myelosuppression and neutropenia in the observation group were significantly decreased, and the immune function and quality of life were significantly improved after treatment (24.0% vs 44.0%, 21.3% vs 44.0%, P < 0.05). CONCLUSION: Shenqi Fuzheng injection in combination with Endostar for AGC is effective, and can significantly improve the immune function and quality of life. © 2015 Baishideng Publishing Group Inc. All rights reserved. Source

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