Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess

Xining, China

Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess

Xining, China
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Li C.,CAS Northwest Institute of Plateau Biology | Li C.,Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess | Li C.,University of Chinese Academy of Sciences | Xiao Y.,CAS Northwest Institute of Plateau Biology | And 8 more authors.
Zhongguo Zhongyao Zazhi | Year: 2011

Objective: To develop an HPLC method for determination of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and pipeline in Tibetan medicine Dangzuo, and to compare the content of four active components in Dangzuo of different Tibetan regions. Method: The separation was carried out on a Waters XTerra RP-C18 column (4.6 mm x 250 mm, 5 μm). The mobile phases were methanol and water, all contained 0.1 % glacial acetic acid, for gradient elution. The gradient program was as follows; 0-22.5 min, methanol was changed from 5% to 50%; 22.5-40 min, changed to 80% 80:20. The flow rate was 1.0 mL·min-1. The detection wavelength was 270 nm. The reference wavelength was 500 nm. Result: The linear ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine were 0.040-0.640 μg (r =0.999 8), 0.090-1.440 μg (r=0.999 9), 0.031-0.500 μg (r=0.999 9) and 0.092-41.477 μg (r=0.998 9), respectively. The average recoveries (n=6) were 97.42% (RSD 1.9%), 97.55% (RSD 2.9%), 98.69% (RSD 0.96%) and 96.72% (RSD 4.0%), respectively. The content ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Dangzuo samples of different Tibetan regions were 0.113-41.69 mg·g-1, 0.889-1.51 mg·g-1, 0.000-40.606 mg·g-1 and 1.96-2.73 mg·g-1, respectively. Conclusion: The method is a simple and effective for quality control of Tibetan medicine Dangzuo.


Li C.,CAS Northwest Institute of Plateau Biology | Li C.,Qinghai Province Key Laboratory of Tibetan Medicine Pharmacology and Safety Assess | Li C.,University of Chinese Academy of Sciences | Yang H.-X.,CAS Northwest Institute of Plateau Biology | And 9 more authors.
Guang Pu Xue Yu Guang Pu Fen Xi/Spectroscopy and Spectral Analysis | Year: 2011

The mercury in Tibetan medicine has become important focus in the research on medicine safety evaluation. The total mercury and the ionic mercury in artificial gastric juice of Tibetan medicine Dangzuo were detected by Gold Amalgam Enrichment-Atomic Fluorescence Spectrometry (GAE-AFS). In the present study, Tibetan medicine Dangzuo was prepared by H2SO4-KNO3 digestion system and artificial gastric juice. The established method and condition of instrument were investigated. Under the optimum experimental conditions and instrumental operation parameters, the recovery (n=6) of HgS is 99.56% (RSD=1.94%), the limit of detection for mercury is 0.2 ng·L-1, the linear range is 0~500 ng·L-1, and r=0.9999. Then, the total mercury and the ionic mercury in artificial gastric juice in Dangzuo samples from different Tibetan regions were assayed. The result showed that the ranges of total mercury and ionic mercury in artificial gastric juice were 3.9980~16.7358 mg·g-1 and 45.5377~1033.9850 ng·g-1, respectively. The analytical method mentioned above is rapid and accurate for determining the amount of mercury in Tibetan medicine Dangzuo.

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