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Ding Z.,Qingdao Women and Childrens Hospital
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery

To investigate the clinical pathological features of tracheobronchial foreign bodies and to improve the diagnosis and treatment. A retrospective study was conducted on 1050 cases with suspected tracheobronchial foreign bodies. Each patient was analyzed for age, sex, history, complication and location of the foreign body. Nine hundred and forty-nine out of 1050 cases were diagnosed as tracheobronchial foreign body, in which 13 patients coughed out the foreign bodies before operation and the rest 936 cases were given operation by bronchoscope under general anesthesia. Among the 949 cases, 936 cases were given operation, with successful removal of foreign bodies in 932 cases (99.6%). One patient suffered from complication (0.1%). For the diagnosis of tracheobronchial foreign bodies, it is very important to collect the detailed history of foreign body inhalation, physical examination and chest roentgenoscopy. Spiral CT with 3-D reconstruction of trachea and bronchus is performed in patients with suspected foreign bodies. Complete surface anesthesia of respiratory tract mucosa is the key procedure in the removal of foreign bodies from respiratory tract by bronchoscope. Source

Fu Y.,Qingdao Women and Childrens Hospital | Sui X.,Qingdao University | Han Y.,Ocean University of China | Feng X.,Ocean University of China
Engineering Technology, Engineering Education and Engineering Management - International Conference on Engineering Technology, Engineering Education and Engineering Management, ETEEEM 2014

An active ingredient glycoprotein by H&F (GHF) was separated from fresh scapharca kagoshimensis, and its physical–chemical properties and anti-tumor activity were researched. Preliminary analysis suggested that it was a kind of glycoprotein; the pharmacology experiment showed that GHF could significantly inhibit the growth of the tumor in the mice with S180, and it showed a good dose–effect relationship. The inhibition rate of GHF on the tumor of transplanted S180 was 60.85%, when the dosage was 200 mg/kg. © 2015 Taylor & Francis Group, London. Source

Yue B.,Qingdao University | Zhang L.,Qingdao Women and Childrens Hospital
European Review for Medical and Pharmacological Sciences

OBJECTIVE: ERK5 is over expressed in a many of human cancers and this overexpression has been associated with metastasis and invasion. Furthermore, ERK5 silencing inhibits aggressive phenotypes of cancer cells. However, mechanisms by which ERK5 regulates tumour progression or metastasis have not been elucidated. In this study, using human osteosarcoma cell lines U2OS as a model, we explored the involvement of ERK5 silencing on invasiveness of U2OS cells. MATERIALS AND METHODS: ERK5 siRNA targeting ERK5 was stably transfected into the human osteosarcoma cell lines U2OS. ERK5 knocked-down U2OS cells was then transfected with Slug cDNAorMMP-9 cDNA plasmidtore-express Slug or MMP-9. Cell proliferation was detected by MTT assay. Cell invasion and metastasis was detected by Matrigel invasion and wound healing assay. An orthotopic nude mouse model of U2OS was applied for in vivo lung metastasis experiments. ERK5, Slug, MMP-9 and E-cadherin were analyzed by realtime PCR, and Western blotting. RESULTS: ERK5 silencing by siRNA in U2OS cells decreased Slug and MMP-9 expression. Compared with the vector-transfected cells, ERK5 knocked-down cells showed reduced migration and invasion in vitro, as well as decreased metastatic potential in experimental metastasis. Re-expression of Slug or MMP-9 in ERK5 knocked-down cells restored the invasive phenotypes. We also discovered that Re-expression of Slug in ERK5 knocked-down cells restored the MMP-9 expression, and re-expression of MMP-9 in ERK5 knocked-down cells did not affect Slug and ERK5 expression. CONCLUSIONS: Our data suggest that ERK5 knockdown inhibits aggressive behaviour of human U2OS cells through modulating Slug signaling and MMP-9 expression. Source

He X.,Qingdao University | Wang L.,Qingdao Women and Childrens Hospital | Li W.,Qingdao University | Yu Z.,Qingdao University | Wang X.,Qingdao University
International Journal of Clinical and Experimental Medicine

Most patients will choose breast reconstruction after breast cancer surgery, while radiotherapy will damage skin and soft tissue so that will have adverse effect on reconstruction. In this study, we assume that the usage of Amifostine can reduce the incidence of complications after breast reconstruction so that provides more choices of reconstruction operation. Dividing SD rats into surgical placement expansion material group (include 15 ml normal saline) and simple operation group. Then further divide the former into non intervention group , radiation group and Radiation therapy combined with Amie amifostine treatment group. The decubation is 45 days after operation. Macroscopic evaluate the complications of skin and soft tissue by ImageJ. There is no obvious complications of skin and soft tissue for control group, radiotherapy alone group and radiotherapy with application of Amifostine group by macroscopic evaluation. The animals that are in expanded object group, damage probability of skin and soft tissue when use Amifostine is lower than that of radiotherapy alone group (30% vs. 69%, P=0.041). ImageJ shows the necrosis probability of skin and soft tissue when use Amifostine is obvious lower than radiotherapy alone group (6.96% vs. 12.94%, P=0.019). In conclusion, prevention and treatment of Amifostine can significantly reduce the complications of skin and soft tissue which is helpful to breast reconstruction after breast cancer surgery. © 2015, E-Century Publishing Corporation. All rights reserved. Source

Ma S.,Shandong University | Hu S.,Shandong University | Gao F.,Qingdao Hiser Hospital | Liang R.,Qingdao Women and Childrens Hospital
Surgical Laparoscopy, Endoscopy and Percutaneous Techniques

Background: Endoscopy lithotomy (percutaneous transhepatic cholangioscopic lithotomy or through a T-tube track) has become a well-established treatment for intrahepatic gallstones. However, the relationship between strictures and the results of this treatment is still controversial. Materials and Methods: We searched PubMed, EMBASE, Cochrane Library databases, clinicaltrails, CBM, and Google scholar systematically for all articles. The rate of complete clearance, the recurrence rate, and major complications were analyzed. A meta-analysis was performed by RevMan 5.2. Results: Thirteen studies were included. In patients with intrahepatic duct stricture, the rate of complete clearance was significantly lower, but the rate of recurrence was significantly higher. The morbidity rate of major complications was significantly lower in complete stone removal patients. Conclusions: Intrahepatic biliary stricture is a major cause of treatment failure for intrahepatic stones and stone recurrence. To prevent recurrent cholangitis or cholangiocarcinoma, complete removal of intrahepatic gallstones is very important. © 2015 Wolters Kluwer Health, Inc. All rights reserved. Source

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