Qingdao Women and Children Hospital

Qingdao, China

Qingdao Women and Children Hospital

Qingdao, China
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Hu L.,Ocean University of China | Sun C.,Ocean University of China | Luan J.,Shandong Entry Exit Inspection and Quarantine Bureau | Lu L.,Qingdao Women and Children Hospital | Zhang S.,Ocean University of China
Acta Biochimica et Biophysica Sinica | Year: 2015

Antioxidants, or anti-oxidant agents, have attracted a great deal of attention in recent years because of their roles in prevention of chronic diseases and utilization as preservatives in food and cosmetics. In this study, we clearly demonstrated that zebrafish recombinant phosvitin (rPv) is an antioxidant agent capable of inhibiting the oxidation of the linoleic acid, and scavenging the 2,2-diphenyl-1-picrylhydrazyl radical. We also showed that zebrafish rPv is a cellular antioxidant capable of protecting radical-mediated oxidation of cellular biomolecules. Importantly, zebrafish rPv is non-cytotoxic to murine macrophage RAW264.7 cells. It is the first report that showed the antioxidant activities of Pv in fishes, suggesting that zebrafish Pv can be an important antioxidant, which can be used as preservatives in food and cosmetics and even as supplementary mediator in different diseased states. © The Author 2015.


PubMed | Eighth Peoples Hospital of Qingdao, Qingdao Women and Children Hospital, Qingdao Hiser Medical Group, Shandong University and Qingdao University
Type: Journal Article | Journal: Archives of gynecology and obstetrics | Year: 2016

Limited studies have reported the effect of prenatal alcohol exposure (PAE) on fetal liver development or liver dysfunction. The current review was conducted to systematically review published studies of PAE and liver dysfunction.Pub Med, Embase and Web of Science database were searched using terms of prenatal alcohol exposure and liver or fetal alcohol spectrum disease and liver. The pooled effect size of alcohol exposure was assessed by Hedgess g and 95% confidence interval (CI) using fixed model or random model depending on the heterogeneity determined by Q test and I (2) statistic.A total of 23 studies were included. The results indicated that gestation alcohol exposure resulted in significant reduction of fetal body weight (Hedgess g=-6.8541.149, 95% CI -9.106 to -4.602, P<0.001), but not fetal liver weight reduction (Hedgess g=-0.0760.878, 95% CI -1.799 to 1.647, P=0.931). PAE resulted in significant decline in protein synthesis or enzyme activity of offspring fetal liver including glutathione and 25(OH)2D (Hedgess g=-1.1490.108, 95% CI -1.361 to -0.938, P<0.001), as well as significant increase in proteins including oxidants and collagen (Hedgess g=1.3300.146, 95% CI 1.044-1.616, P<0.001).These results suggested that PAE affects fetal body weight but not liver weight, and that PAE may result in offspring fetal liver dysfunction.


PubMed | Shandong Provincial Hospital, Qingdao Women and Children Hospital, Qingdao Hiser Medical Group, Eighth Peoples Hospital of Qingdao and Qingdao University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

In this case-control study, we assessed the influence of IL-10 -1082A/G and -819T/C on the development of preeclampsia. The IL-10 -1082A/G and -819T/C polymorphisms were assessed by polymerase chain reaction-restriction fragment length polymorphism. The genotype distributions of the IL-10 -1082A/G and -819T/C polymorphisms in the control subjects were in conformance with Hardy-Weinberg equilibrium (HWE; P = 0.46 and 0.17). Unconditional logistic regression analyses revealed that individuals carrying the CC genotype of IL-10 -819T/C were associated with an increased risk of preeclampsia compared to the TT genotype. The odds ratio (95% confidence interval) for the CC genotype of IL-10 -819T/C was 1.71 (1.07-3.27) compared to the TT genotype. In conclusion, the results of our study indicated that the IL-10 -819T/C polymorphism was associated with an increased risk of preeclampsia in a Chinese population.


Hui L.,Qingdao Women and Children Hospital | Lei Z.,Qingdao Women and Children Hospital | Peng Z.,Qingdao Women and Children Hospital | Ruobing S.,Qingdao Women and Children Hospital | Fenghua Z.,Qingdao Women and Children Hospital
Pakistan Journal of Pharmaceutical Sciences | Year: 2014

To investigate the correlation between cytotoxic T lymphocyte antigen-4 (CTLA-4) gene polymorphism and children with acute lymphoblastic leukemia (ALL). A total of 86 children of ALL (23 HR, 54SR) and 112 healthy controls was selected. The genptypes were determined by means of polymerase chain reaction (PCR) and the PCR product sequencing. Genotype and alleles frequency of SNP-318, SNP+49 and SNP-CT60 were compares among different groups. The frequency of TC,TT genotype and T allele in ALL children at SNP-318 position were statistically higher than controls. In HR group, the frequency of TC, TT genotype at SNP-318 position was statistically higher than SR group. There was no significantly difference in genotype and allele distribution of SNP+49 position among the HR patients, SR patients and control group. (2) The frequency of GG genotype and G allele in ALL children at SNP-CT60 position were significantly higher than controls. The genotype and allele distribution of SNP-CT60 position between different clinical risk groups were no significantly different. As a result of the increased frequency of TC, TT genotype and T allele at SNP-318, ALL children synthesized more CTLA-4 to deliver the inhibitive signal, and this lead to restraint of T cell activation. Such difference at SNP-318 position was obvious in HR children. The SNP+49 position is probably not the main regulating point in ALL. (2) In SNP-CT60 position, the G allele played the main part. The increase of G allele frequency result in the high expression of CTLA-4. such difference at SNP-318 position was obvious in HR children.


Jiao B.-Y.,Qingdao Women and Children Hospital | Han Y.-N.,Qingdao Women and Children Hospital | Ding Y.,Qingdao Women and Children Hospital
Academic Journal of Second Military Medical University | Year: 2017

Objective To investigate the incidence rates of different fetal variations of inferior vena cava, and to compare the diameters and peak velocity of blood in the inferior vena cava between the normal and changed inferior vena cava. Methods Prenatal ultrasound data of 37 448 fetuses were retrospectively analyzed. We screened out the cases with variation of inferior vena cava, and calculated related incidence. Then the diameters and S-wave peak velocity of the inferior vena cava in fetuses of the two groups were measured and compared. Results We found 54 (1.44‰) cases of left inferior vena cava and 14(0.37‰) cases of double inferior vena cava. The diameter of normal fetal inferior vena cava was (2.71±0.42) mm and the peak velocity was (36.25±6.30) cm/s, the diameter of the left inferior vena cava was (2.70±0.45) mm and the peak velocity was (36.55±7.53) cm/s, both showing no significant difference when compared with the normal group (t=6.78, P>0.05; t=3.56, P>0.05). The diameter of double inferior vena cava was (2.72±0.50) mm and the peak velocity was (36.18±7.49) cm/s, showing no significant difference when compared with the normal group (t=10.43, P>0.05; t=7.01, P>0.05). Conclusion The incidence rates of left inferior vena cava and double inferior vena cava in fetuses have been found to be 1.44‰ and 0.37‰ respectively- and they have similar diameters and velocity with normal inferior vena cava. © 2017, Second Military Medical University Press. All rights reserved.


Song H.,Qingdao Women and Children Hospital
European review for medical and pharmacological sciences | Year: 2016

OBJECTIVE: Medulloblastoma is the most common malignant brain tumor in children. Despite remarkable advances over the past decades, a novel therapeutic strategy is urgently required to increase long-term survival. This study aimed to understand the role of a long non-coding RNA (lncRNA), colorectal neoplasia differentially expressed (CRNDE), in medulloblastoma tumor growth.MATERIALS AND METHODS: The transcript level of CRNDE was initially examined in dissected clinical tissues and cultured cancerous cells. Effects of CRNDE knockdown on cell viability and colony formation in vitro were assessed using the CCK-8 and colony formation assays, respectively. Cell cycle progression and survival were also determined after CRNDE knockdown. A xenograft mouse model of human medulloblastoma was established by injecting nude mice with medulloblastoma cells stably depleted of CRNDE expression.RESULTS: Our data suggest that transcript levels of CRNDE are elevated in clinical medulloblastoma tissues instead of in adjacent non-cancerous tissues. Knockdown of CRNDE significantly slowed cell proliferation rates and inhibited colony formation in Daoy and D341 cells. Tumor growth in vivo was also inhibited after CRNDE knockdown. Moreover, after knockdown of CRNDE, cell cycle progression was arrested in S phase and apoptosis was promoted by 15-20% in Daoy and D341 cells. In vivo data further showed that proliferating cell nuclei antigen (PCNA) was decreased, whereas the apoptosis initiator cleaved-caspase-3 was increased upon CRNDE knockdown in cancerous tissues from the mouse model.CONCLUSIONS: All these data suggest that CRNDE promotes tumor growth both in vitro and in vivo. This growth-promotion effect might be achieved via arresting cell cycle progression and inhibiting apoptosis. Therapeutics against CRNDE may be a novel strategy for the treatment of medulloblastoma.


Mo X.-M.,Ocean University of China | Mo X.-M.,Qingdao Women and Children Hospital | Li H.-H.,Qingdao University | Li H.-H.,Qingdao Municipal Hospital | And 2 more authors.
Oncology Letters | Year: 2014

In order to study the influence and mechanism of miR-544a on the self-renewal ability of lung cancer stem cells, TargetScan was used to predict the target gene of miR-544a. A luciferase reporter system and western blotting were used to validate the target genes identified by TargetScan. 95C and 95D low and high metastatic human lung cancer cells were transfected with miR-544a, and quantitative polymerase chain reaction (qPCR) was used to verify the miR-544a expression in these two cell lines. Tumor ball (spheroid) suspension culture was use to study the effects of miR-544a on lung cancer stem cells. TargetScan predicted that miR-544a interacted with GSK3β. A luciferase reporter system (F=201.37, P<0.01) and western blot analysis was used to validate that miR-544a could inhibit the expression of GSK3β, while β-catenin and CD133 were significantly increased in miR-544a-overexpressing 95C and 95D cells (F=9.43, 7.73 and 3.37, respectively; P<0.01). qPCR revealed that miR-544a was overexpressed in transfected 95C and 95D cells (20.51±0.97 and 15.16±1.38, respectively; F=418.05; P<0.01). miR-544a-overexpressing cells formed spheroids in suspension cultures of spheroid single cells. miR-544a was shown to reduce the expression of GSK3β and activate the Wnt signaling pathway to maintain the self-renewal ability of lung caner stem cells.


Mo X.,Ocean University of China | Mo X.,Qingdao Women and Children Hospital | Zhang F.,Qingdao Women and Children Hospital | Liang H.,Qingdao Women and Children Hospital | And 3 more authors.
OncoTargets and Therapy | Year: 2014

Objective: To find out the effect of miR-544a on the invasion of lung cancer cells and to explore the underlying molecular mechanisms. Methods: Micro-ribonucleic acid (miRNA) expression in two different invasive lung cancer cell lines 95C (low invasive ability) and 95D (high invasive ability) was analyzed by miRNA microarray and real-time quantitative polymerase chain reaction (PCR); miR-544a mimic was transfected to 95C, and its invasion ability was detected by transwell migration assay; we predicted the candidate miRNA target genes by TargetScan (Whitehead Institute for Biomedical Research, Cambridge, MA, USA) software and verified the target genes by Western blot. Results: The expression of miR-544a was significantly increased in 95D in miRNA microarray and quantitative PCR tests (P,0.05). After being transfected with miR-544a mimic, the invasion ability of 95C was enhanced (P,0.01). Moreover, transfection with miR-544a inhibitor decreased the invasion ability of 95D (P,0.01). miR-544a possibly combined with CDH1 (E-cadherin) predicted by the TargetScan analysis. 95C with miR-544a mimic reduced the expression of CDH1 and improved the expression of vimentin, while 95D with miR-544a inhibitor improved the expression of CDH1 and reduced the expression of vimentin. Conclusion: miR-544a can promote the invasion of non-small cell lung cancer by downregulation of CDH1 and upregulation of vimentin. © 2014 Mo et al. This work is published by Dove Medical Press Limited.


PubMed | Qingdao Women and Children Hospital and Qingdao University
Type: Journal Article | Journal: Systems biology in reproductive medicine | Year: 2016

Estradiol mediates its actions by binding to classical nuclear receptors, estrogen receptor (ER-) and estrogen receptor (ER-), and the non-classical G protein-coupled estrogen receptor 1(GPER). Several gene knockdown models have shown the importance of the receptors for growth of the oocyte and for ovulation. The aim of our study was to identify the pattern of GPER expression in human cumulus granulosa cells (CGCs) from ovarian follicles at different stages of oocyte maturation, and the differences of GPER expression between polycystic ovary syndrome (PCOS) patients and non-PCOS women. Thirty-eight cases of PCOS patients and a control group of thirty-two infertile women without PCOS were used in this study. GPERs location in CGCs was investigated by immunohistochemistry. Quantitative RT-PCR and western blot were used to identify the quantify GPER expression. Here we demonstrated that GPER was expressed in CGCs of both PCOS patients and non-PCOS women, and the expression of GPER was decreased significantly during oocyte maturation. But the expression levels of GPER in CGCs of PCOS patients and non-PCOS women were not significantly different. The data indicate that GPER may play a role during human oocyte maturation through its action in cumulus granulosa cells.AMHRIIs: anti-Mullerian hormone type II receptors; BMI: body mass index; CGCs: cumulus granulosa cells; COH: controlled ovarian hyperstimulation; E2: estradiol; EGFR: epidermal growth factor receptor; ER-: estrogen receptor; ER-: estrogen receptor ; FF: follicular fluid; FSH: follicle-stimulating hormone; GCs: granulosa cells; GPER: G protein-coupled estrogen receptor 1; GV: germinal vesicle; GVBD: germinal vesicle breakdown; HCG: human chorionic gonadotropin; IRS: immunoreactive score; IVF-ET: in vitro fertilization and embryo transfer; MI: metaphase I; MII: metaphase II; MAPK: mitogen-activated protein kinase; OCCCs: oocyte corona cumulus complexes; PCOS: polycystic ovarian syndrome; qquantitative real-time PCR: qRT-PCR.


Yu D.,Qingdao Women and Children Hospital | Han Y.,Qingdao Women and Children Hospital | Chen T.,Qingdao Women and Children Hospital
Otolaryngology - Head and Neck Surgery (United States) | Year: 2014

Objective. Contrast-enhanced ultrasound (CEUS) is a new noninvasive modality for the diagnosis of thyroid nodules. However, the performance of CEUS in differentiating malignant and benign thyroid nodules has not been systematically evaluated. This meta-analysis was performed to assess the accuracy of CEUS in diagnosing thyroid nodules.Data Sources. PubMed, Embase, and the references of included studies were examined.Review Methods. We recorded the characteristics of the included studies and assessed the quality of each study using the Quality Assessment of Diagnostic Accuracy Studies tool. The pooled sensitivity, specificity, positive likelihood ratio (LR), negative LR, diagnostic odds ratio (DOR), and area under the curve (AUC) were calculated. We also evaluated the publication bias.Results. This meta-analysis included 7 studies with a total of 597 thyroid nodules. The pooled the sensitivity, specificity, and positive and negative LR were 0.853, 0.876, 5.822, and 0.195, respectively. The DOR and AUC were 34.730 and 0.9162, respectively. Heterogeneity existed between the included studies. The results of subgroup analyses indicated that the evaluation processes are likely the predominant source of heterogeneity. No significant publication bias was observed.Conclusion. Contrast-enhanced ultrasound is a promising noninvasive technique for the differential diagnosis of benign and malignant thyroid nodules and could be a valuable supplemental method to fine-needle aspiration. © American Academy of Otolaryngology - Head and Neck Surgery Foundation 2014.

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