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Zhang R.,State Oceanic Administration | Chen J.,State Oceanic Administration | Shi Q.,State Oceanic Administration | Shi Q.,Ocean University of China | And 5 more authors.
Journal of Separation Science | Year: 2014

Flavonoids are the main active components of natural medicinal plants with many physiological functions. In this study, an HPLC fingerprinting method based on the distribution and relative amount of 11 bioactive flavonoids was established for the quality evaluation of commercially available wild Jujube leaf tea (JLT) from China. Separation of the crude flavonoid extract was achieved on a column filled with C18 material with a high carbon content. The flavonoids in wild JLT were identified based on UV spectroscopy and accurate mass measurements by TOF-MS. Twenty-one batches of practical samples collected from different habitats were analyzed by using the developed HPLC method to construct the HPLC characteristic fingerprint of wild JLT. Then, combined with clustering and similarity analyses, the HPLC characteristic fingerprint was used for the authentication and quality evaluation of commercial wild JLT. Results indicated that the proposed HPLC characteristic fingerprint reflected the inherent characteristics of wild JLT collected from different regions. Authenticity identification and quality control of commercially available wild Jujube tea were achieved based on the HPLC characteristic fingerprint analysis. This new approach to bioactive component profiling provided a promising reference method for the quality evaluation of commercial wild flower and plant tea. © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Zhang R.,State Oceanic Administration | Chen J.,State Oceanic Administration | Shi Q.,State Oceanic Administration | Shi Q.,Ocean University of China | And 5 more authors.
Food Research International | Year: 2014

Ziziphus jujube leaf tea (ZJLT. 11ZJLT is the abbreviation of Ziziphus jujube leaf tea.) is a new type of plant tea that has recently gained popularity in China. ZJLT is often advertised by its manufacturer for its special capacity to improve sleep. However, the suitability of drinking ZJLT at night still lacks scientific basis. This work aims to identify the typical phytochemical components in ZJLT infusion and try providing a scientific basis for drinking at night. An efficient liquid chromatography-diode array detector with time-of-flight mass spectrometry (LC-DAD-TOF/MS) method and online 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay were performed to clarify the principal bioactive compounds of ZJLT. First, caffeine and theophylline, which have important functions in refreshing the human body, were not detected in the ZJLT infusion. Second, the ZJLT infusion contains rich flavonoids, mainly flavonol glycosides, with possible sedative and hypnotic activities. Finally, a number of free radical scavengers were screened out in the ZJLT infusion. In this research, the comprehensive bioactive component information showed that ZJLT does not contain refreshing components; rather, ZJLT contains rich possibly sedative flavonoids and antioxidants. Hence, based exclusively on its chemical composition, ZJLT may be suitable for drinking at night. © 2013 Elsevier Ltd. Source

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