Qingdao University of Science and Technology is a university located in Qingdao, China, colloquially known as Qingkeda . Having evolved from a college specializing in the chemical industry, it is now a comprehensive, multi-disciplinary university offering academic degrees in Science, Technology, Humanities, Business, Management, Medicine, Law and Arts. The school encompasses three campuses in Sifang and Laoshan districts of Qingdao and Gaomi district of Weifang. Wikipedia.
Qingdao University of Science and Technology | Date: 2014-11-05
A single-screw extrusion desulfurization and post-processing system and a method for preparing reclaimed rubber. The single-screw extrusion desulfurization and post-processing system comprises: a single-screw desulfurization device, a single-screw post-processing device and a closed connection device for connecting the single-screw desulfurization device and the single-screw post-processing device, the single-screw post-processing device includes a post-processing feeding unit and a post-processing unit which are connected with each other, and a post-processing screw running through the post-processing feeding unit and the post-processing unit.
Zhang X.-W.,Qingdao Technological University |
Zhang Z.-X.,Qingdao University of Science and Technology
Toxicon | Year: 2012
A new method was developed to quantify domoic acid by capillary electrophoresis-based enzyme immunoassay (CE-EIA) with electrochemical (EC) detection. The method was based on noncompetitive immunoreaction between free domoic acid antigen (Ag) and excessive amount of horseradish peroxidase (HRP)-labeled antidomoic acid antibody tracer (Ab*) in liquid phase. Then the bound enzyme-labeled complex (Ab*-Ag) and unbound Ab* were separated by CE, and the system of HRP catalyzing H 2O 2/o-aminophenol (OAP) reaction was adopted for EC detection. Using CE-EIA with EC detection, equilibrium was reached in 30min, and the analytical results were obtained within a further 5min. The linear range and the detection limit were 0.1-50ng/mL and 0.02ng/mL, respectively. Analytes recoveries were 89.6-105.8%. The sensitivity of the method was 16 times greater than that of enzyme-linked immunosorbent assay (ELISA). The developed method was applied to quantitatively analysis of domoic acid in contaminated shellfish samples with rapid and simple pretreatment, and the results were consistent with the same samples analyzed through ELISA. The CE-EIA with EC detection provides a valid and sensitive analytical approach, not previously available, for determination of domoic acid in shellfish samples. © 2012 Elsevier Ltd.
Wang Y.,Tsinghua University |
Chen C.,Tsinghua University |
Peng J.,Tsinghua University |
Li M.,Qingdao University of Science and Technology
Angewandte Chemie - International Edition | Year: 2013
Three become one: Multiply substituted quinolines were synthesized from diaryliodoniums, alkynes, and nitriles by a CuII-catalyzed method. This cascade annulation is highly regioselective, step-economic, flexible with regard to the functional groups, and could potentially be applied to the synthesis of complex molecules. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Shen Y.,CAS Beijing National Laboratory for Molecular |
Fu X.,CAS Beijing National Laboratory for Molecular |
Fu W.,CAS Beijing National Laboratory for Molecular |
Li Z.,Qingdao University of Science and Technology
Chemical Society Reviews | Year: 2015
The stimuli-responsive polypeptides have drawn extensive attention because of their promising applications in biotechnology considering their biocompatibility, biodegradability, and bioactivity. In this tutorial review, we summarize the most recent progress in this area, including thermo-, redox-, photo-, and biomolecule responsive polypeptides over the past decade. The design and synthesis of stimuli-responsive polypeptides will be briefly introduced. The correlation between the structure and properties, particularly the effects of polypeptide conformation, will be emphasized here. In addition, the applications of stimuli-responsive polypeptides in controlled drug release and tissue engineering are briefly discussed. © The Royal Society of Chemistry 2015.
Luo X.,Qingdao University of Science and Technology |
Luo X.,University of Oxford |
Davis J.J.,University of Oxford
Chemical Society Reviews | Year: 2013
Electrical detection methodologies are likely to underpin the progressive drive towards miniaturised, sensitive and portable biomarker detection protocols. In being easily integrated within standard electronic microfabrication formats, and developing capability in microfluidics, the facile multiplexed detection of a range of proteins in a small analytical volume becomes entirely feasible with something costing just a few thousand pounds and benchtop or handheld in scale. In this review, we focus on recent important advances in label free assays of protein using a number of electrical methods, including those based on electrochemical impedance spectroscopy (EIS), amperometry/voltammetry, potentiometry, conductometry and field-effect methods. We introduce their mechanistic features and examples of application and sensitivity. The current state of the art, real world applications and challenges are outlined. © 2013 The Royal Society of Chemistry.
Wan J.,Qingdao University of Science and Technology
Nanotechnology | Year: 2010
Bifunctional nanoprobes with both magnetic and optical contrast have been developed for ultra-sensitive brain tumor imaging at the cellular level. The nanoprobes were synthesized by simultaneously incorporating a magnetite nanoparticle cluster and fluorescence dyes into silica encapsulation by a sol-gel approach under ultrasonic treatment. The nanoprobes maintain superparamagnetic behavior at room temperature and possess enhanced transverse relaxivity and good photostability. As a glioma targeting ligand, chlorotoxin was covalently bonded to the surface of the nanoprobes. In vitro cellular uptake assays demonstrated that the nanoprobes were highly specific, taken up by human U251-MG glioma cells via receptor-mediated endocytosis. The labeled glioma cells were readily detectable by both MR imager and confocal laser scanning microscopy.
Bi S.,Qingdao University of Science and Technology |
Zhao T.,Qingdao University of Science and Technology |
Luo B.,Qingdao University of Science and Technology
Chemical Communications | Year: 2012
The bifunctionality of graphene oxide (GO) that can highly adsorb single-stranded DNA (ssDNA) and effectively quench the emission of organic dyes is reasonably utilized in a chemiluminescence resonance energy transfer (CRET) system, achieving sensitive and selective detection of DNA (H1V1) and protein (thrombin), respectively. © 2012 The Royal Society of Chemistry.
Jie G.,Qingdao University of Science and Technology
Chemistry (Weinheim an der Bergstrasse, Germany) | Year: 2011
Magnetic electrochemiluminescent Fe(3)O(4)/CdSe-CdS nanoparticle/polyelectrolyte nanostructures have been synthesized and used to fabricate an electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA). CEA is a protein used as a biomarker for several cancers; particularly, to monitor response to treatment in colon and rectal cancer patients. The nanocomposites can be easily separated and firmly attached to an electrode owing to their excellent magnetic properties. This represents a promising advantage for bioassay applications. More importantly, the nanostructures exhibit intense and stable ECL emissions in neutral solution, which makes them ideal for ECL immunosensing. The 3-aminopropyltriethoxysilane (APS) polyelectrolyte shell on the nanostructure surface not only enhances the intensity and stability of the ECL signal, but also acts as a crosslinker for immunosensor fabrication. A CEA antibody immobilized onto a nanocomposite/APS/electrode with gold nanoparticles comprises the ECL immunosensor. The principle of ECL detection for CEA is based on a change in steric hindrance after immunoreaction, which leads to a decrease in ECL intensity. A wide detection range (0.064pg ml(-1)-10ng ml(-1)) and low detection limit (0.032pg ml(-1)) are achieved. The immunosensor is highly sensitive and selective, and exhibits excellent stability and good reproducibility. It thus has great potential for clinical protein detection. In particular, this approach uses a novel class of bifunctional nanocomposites that display both intense ECL and excellent magnetism, which renders them suitable for a large range of bioassay applications. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Bi S.,Qingdao University of Science and Technology |
Li L.,Qingdao University of Science and Technology |
Cui Y.,Qingdao University of Science and Technology
Chemical Communications | Year: 2012
The network consisting of three kinds of unlabeled stem-loop DNA molecular beacons (MBs) is activated by target DNA in the presence of exonuclease-III (Exo-III), achieving the concept of exonuclease-assisted cascaded recycling amplification (Exo-CRA) for DNA detection with a wide dynamic range of 8 orders of magnitude. © 2012 The Royal Society of Chemistry.
Jie G.,Qingdao University of Science and Technology |
Yuan J.,Qingdao University of Science and Technology
Analytical Chemistry | Year: 2012
A novel small magnetic electrochemiluminescent Fe 3O 4@CdSe composite quantum dot (QD) was facilely prepared and successfully applied to sensitive electrochemiluminescence (ECL) detection of thrombin by a multiple DNA cycle amplification strategy for the first time. The as-prepared composite QDs feature intense ECL, excellent magnetism, strong fluorescence, and favorable biocompatibility, which offers promising advantages for ECL biosensing. ECL of the composite QDs was efficiently quenched by gold nanoparticles (NPs). Taking advantages of the unique and attractive ECL and magnetic characteristics of the composite QDs, a novel DNA-amplified detection method based on ECL quenching was thus developed for a sensitive assay of thrombin. More importantly, the DNA devices by cleavage reaction were cycled multiple rounds, which greatly amplified the ECL signal and much improve the detection sensitivity. This flexible biosensing system exhibits not only high sensitivity and specificity but also excellent performance in real human serum assay. The present work opens a promising approach to develop magnetic quantum dot-based amplified ECL bioassays, which has wider potential application with more favorable analytical performances than other ECL reagent-based systems. Moreover, the composite QDs are suitable for long-term fluorescent cellular imaging, which also highlights the promising directions for further development of QD-based in vitro and in vivo imaging materials. © 2012 American Chemical Society.