Qingdao Tumor Hospital

Qingdao, China

Qingdao Tumor Hospital

Qingdao, China
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PubMed | Qingdao Tumor Hospital, Shanghai JiaoTong University and Shandong University
Type: | Journal: Colloids and surfaces. B, Biointerfaces | Year: 2016

The clinical usage of docetaxel (DTX) has been blocked in the clinic because of its poor solubility and tumour multi-drug resistance (MDR). The dominating mechanism of MDR is the over-expression of p-gp on tumour cells. Traditional nano-medicines, such as nanoparticles and micelles, have been used to physically entrap DTX to improve their solubility, while the drug loading content was very low and the tumour resistance was neglected. In this study, the synthesized reduction-sensitive mPEG-PLGA-SS-DTX conjugate was utilized to load the p-gp inhibitor veraparmil (VRP) to prepare DTX and VRP co-delivered mPEG-PLGA-SS-DTX/VRP (PP-SS-DTX/VRP) multi-functional micelles to reverse MDR and enhance the anti-tumour effect of DTX. The micelles had a high drug loading content and showed an obvious reduction-sensitive release property for both DTX and VRP. In addition, an in vitro anti-tumour assay revealed that the micelles markedly inhibited the efflux activity of p-gp and accelerated cell apoptosis, resulting in the improvement of anti-tumour activity and reversal of MDR. The PP-SS-DTX micelles markedly enhanced the in vivo circulation time and increased the drug accumulation in tumour tissues. Therefore, the PP-SS-DTX/VRP micelle is a desirable drug delivery system for multi-drug resistance therapy of DTX and is very promising for clinical usage.


Huang P.-Y.,Sun Yat Sen University | Sun Z.-Y.,Qingdao Tumor Hospital | Xie C.-M.,Sun Yat Sen University | Chen Q.-Y.,Sun Yat Sen University | And 7 more authors.
Acta Oto-Laryngologica | Year: 2012

Conclusion: Advanced parapharyngeal space (PPS) involvement showed stronger prognostic value than PPS involvement. The classification of PPS involvement proposed by Min or Sham was the most appropriate classification for parapharyngeal extension in nasopharyngeal carcinoma (NPC). The degree of tumor extension into the PPS should be considered in future TNM staging revisions. Objectives: This study was conducted to evaluate the prognostic significance of the various classifications for PPS involvement in patients with NPC. Methods: From January to July 2000, a total of 176 patients with pathologically diagnosed NPC were prospectively enrolled in this study. The extent of PPS involvement was examined by contrast-enhanced computed tomography (CT) scan and graded according to the four previously reported classifications (Min, Sham, Xiao, and Heng). Results: The incidence of PPS involvement was 81.8%. The 5-year overall survival (OS), progression-free survival (PFS), distant metastasis-free survival (DMFS), and local relapse-free survival rates (LRFS) of the patients with and without PPS involvement were 68.1% and 90.2% (p 0.010), 66.1% and 87.2% (p 0.013), 76.7% and 93.6% (p 0.032), and 84.9% and 93.1% (p 0.220), respectively. Multivariate analysis showed that PPS involvement (yes vs no) was not an independent prognostic factor. However, graded PPS involvement was an independent factor affecting the prognosis of NPC. When the four classifications were included in a Cox model, it was shown that PPS involvement based on Min's classification was an independent factor for OS (p 0.001). PPS involvement based on Sham's classification was an independent factor for PFS (p 0.010) and DMFS (p 0.009). © 2012 Informa Healthcare.


Chen X.,CAS Qingdao Institute of Oceanology | Yang S.,Qingdao Tumor Hospital | Wang J.,CAS Qingdao Institute of Oceanology | Song L.,CAS Qingdao Institute of Oceanology | And 4 more authors.
BioMed Research International | Year: 2015

Sulfated polysaccharides (GSP) were isolated from the cloned Grateloupia filicina which was cultured in Jiaozhou Bay, Qingdao, China. The yield of GSP was 15.75%. The total sugar and sulfate were 40.90 and 19.89%, respectively. And the average molecular weight was 11.7 KDa. The results of neutral sugar analysis showed that GSP was mainly sulfated polysaccharides of galactose. The experiments for activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) anticoagulant assays in vitro indicated that GSP was a good potential anticoagulant. Therefore, this study supplied new thought for the cloned Grateloupia filicina exploitation of high-value products. © 2015 Xiaolin Chen et al.


To compare the efficacy and side effects of induction chemotherapy with vinorelbine plus cisplatin (NP) or docetaxel plus cisplatin (TP) combined with concurrent chemoradiotherapy in treating locally advanced nasopharyngeal carcinoma (NPC). From January 2005 to December 2009, 146 patients with locally advanced nasopharyngeal carcinoma treated in our department were randomized into NP group (76 patients) or TP group (70 patients). Both groups received two cycles of induction chemotherapy and concurrent chemoradiotherapy. After three weeks of induction chemotherapy, the patients received concurrent chemoradiotherapy. The chemotherapy was recycled every three weeks. Two groups were treated with intensity-modulated radiation therapy. The short-term efficacy of NP group was similar to that of TP group. The 3-year overall survival rates, disease-free-survival rates, locoregional relapse-free survival rates and distant metastasis-free survival rates in the NP and TP groups were 84.2% and 82.9%, 71.1% and 74.3%, 89.5% and 91.4%, 81.6% and 77.1%, respectively (P > 0.05). The occurrence rates of leucopenia, anemia and acute mucositis were significantly higher in the TP group than those in the NP group (P < 0.05). The gastrointestinal toxicity, dermatitis and liver toxicity were similar in the two groups. The efficacy of NP regimen induction chemotherapy plus concurrent chemordiotherapy for advanced NPC is similar to that of TP regimen. The toxicity of the NP regimen is lower than that of NP regimen, tolerable, and with a good compliance.


Liu H.,Qingdao Tumor Hospital | Ma H.F.,Qingdao Tumor Hospital | Chen Y.K.,Qingdao Tumor Hospital
Genetics and Molecular Research | Year: 2015

The relationship between glutathione S-transferase M1 (GSTM1) genetic polymorphisms and lung cancer has been reported previously. However, the results are not consistent. Therefore, to clarify the association between GSTM1 polymorphisms and lung cancer, we performed a meta-analysis based on published studies. We used the Revman 5.0 software to perform literature retrieval, article selection, data collection, and statistical analysis. We utilized a random-effect model to pool the odds ratios (ORs) and 95% confidence intervals (CIs). A total of 38 eligible studies including 5737 lung cancer patients and 6843 cancer-free control subjects were analyzed. We found no association between GSTM1 genetic polymorphisms and lung cancer risk (OR = 1.15, 95%CI = 0.98-1.36, P = 0.08). Including only Chinese individuals, we found no association between GSTM1 genetic polymorphisms and lung cancer risk (OR = 1.13, 95%CI = 0.97-1.32, P = 0.13). In conclusion, we found that GSTM1 polymorphisms are not associated with lung cancer risk. ©FUNPEC-RP www.funpecrp.com.br


Xu W.,Zhejiang University | Chen Y.,Zhejiang University | He W.,Qingdao Tumor Hospital | Fu Z.,Zhejiang University | And 6 more authors.
Discovery Medicine | Year: 2014

To find new biomarkers and establish histopathology protein fingerprint models for early detection of colorectal cancer (CRC), laser capture microdissection (LCM) was utilized to obtain 3 groups of cells of interest -- CRC tissues, their adjacent normal colorectal tissues, and their adjacent adenomatous polyps tissues -- from the same 12 CRC patients. Each sample was then detected by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology and CM10 protein chip as well as bioinformatics tools. Model 1 formed by 15 protein peaks could be used to distinguish CRC tissues from normal tissues. The diagnostic pattern constructed using support vector machine (SVM) including the 15 proteins showed maximum Youden index (YI). Model 2 formed by 14 protein peaks could be used to distinguish CRC tissues from adenomatous polyps tissues. The two patterns were validated and the results showed that the sensitivity and specificity were both 100.0%. Model 3 formed by 15 protein peaks could distinguish adenomatous polyps tissues from normal tissues with a sensitivity of 92.31% and specificity of 100%. The protein peaks m/z 3570 and 5224 were identified in screening changes during cancer progression. Peak 5224 was significantly upregulated in CRC. However, peak 3570 was significantly downregulated in CRC. LCM technology coupled with SELDI protein chip and bioinformatics approaches could effectively screen the differentially expressed protein profiles and establish molecular diagnosis models with high sensitivity and specificity for CRC. © 2015, Discovery Medicine.


To compare the efficacy and side effects of induction chemotherapy with vinorelbine plus cisplatin (NP) or docetaxel plus cisplatin (TP) combined with concurrent chemoradiotherapy in treating locally advanced nasopharyngeal carcinoma (NPC).From January 2005 to December 2009, 146 patients with locally advanced nasopharyngeal carcinoma treated in our department were randomized into NP group (76 patients) or TP group (70 patients). Both groups received two cycles of induction chemotherapy and concurrent chemoradiotherapy. After three weeks of induction chemotherapy, the patients received concurrent chemoradiotherapy. The chemotherapy was recycled every three weeks. Two groups were treated with intensity-modulated radiation therapy.The short-term efficacy of NP group was similar to that of TP group. The 3-year overall survival rates, disease-free-survival rates, locoregional relapse-free survival rates and distant metastasis-free survival rates in the NP and TP groups were 84.2% and 82.9%, 71.1% and 74.3%, 89.5% and 91.4%, 81.6% and 77.1%, respectively (P > 0.05). The occurrence rates of leucopenia, anemia and acute mucositis were significantly higher in the TP group than those in the NP group (P < 0.05). The gastrointestinal toxicity, dermatitis and liver toxicity were similar in the two groups.The efficacy of NP regimen induction chemotherapy plus concurrent chemordiotherapy for advanced NPC is similar to that of TP regimen. The toxicity of the NP regimen is lower than that of NP regimen, tolerable, and with a good compliance.


PubMed | Qingdao Tumor Hospital and Shandong University
Type: Journal Article | Journal: International journal of pharmaceutics | Year: 2016

The main cause of multidrug resistance (MDR) is overexpression of active efflux transporters, such as P-glycoprotein (P-gp). To reverse MDR and improve the chemotherapy effect of paclitaxel (PTX), we propose a new drug delivery system based on mixed micelles constructed with d--tocopheryl poly(ethylene glycol) 1000 succinate (TPGS) and the mPEG-SS-PTX conjugate with consideration that TPGS is a P-gp inhibitor that can block the cancer cell action of pumping drugs outside of cells and can enhance the anticancer effect. mPEG-SS-PTX is synthesized by conjugating hydrophilic mPEG with a hydrophobic drug, PTX, via a redox-sensitive disulfide bond. The mPEG-SS-PTX conjugate is amphiphilic and can self-assemble in water. Mixed micelles formed by the mPEG-SS-PTX conjugate and TPGS have a low critical micelle concentration (CMC, 1.0510


PubMed | Qingdao Tumor Hospital
Type: Journal Article | Journal: Zhongguo fei ai za zhi = Chinese journal of lung cancer | Year: 2010

PET is a sensitive and special method of determining malignant degree of the lung cancer. This study investigates the correlation between (18)F-deoxyglucose (FDG) uptake and various biological markers in clinical stage I non-small cell lung cancer (NSCLC).The non-small cell lung cancer specimens of 23 patients were examined by flow cytometry and immunohistochemistry, the relationship between the max standard uptake value (SUV) of FDG and the expression of the biological markers p53, Ki-67, proliferating cell nuclear antigen (PCNA), vascular endothelial growth factor (VEGF), glucose transporter-1 (GLUT-1), S-phase fraction (SPF) were studied.The max SUV was correlated with the expression of p53 (P <0.05), Ki-67 (P <0.05), PCNA (P <0.05), VEGF (P <0.05) and SPF (P <0.05) in stage I NSCLC. Glut-1 expression showed significant correlation with FDG uptake (P =0.001), however the max SUV showed no correlation with tumor pathology.Our study indicates that (18)F-FDG uptake in NSCLC correlates well with the expression of p53, Ki-67, PCNA, VEGF, GLUT-1, SPF.


PubMed | Qingdao Tumor Hospital
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2015

The relationship between glutathione S-transferase M1 (GSTM1) genetic polymorphisms and lung cancer has been reported previously. However, the results are not consistent. Therefore, to clarify the association between GSTM1 polymorphisms and lung cancer, we performed a meta-analysis based on published studies. We used the Revman 5.0 software to perform literature retrieval, article selection, data collection, and statistical analysis. We utilized a random-effect model to pool the odds ratios (ORs) and 95% confidence intervals (CIs). A total of 38 eligible studies including 5737 lung cancer patients and 6843 cancer-free control subjects were analyzed. We found no association between GSTM1 genetic polymorphisms and lung cancer risk (OR = 1.15, 95%CI = 0.98-1.36, P = 0.08). Including only Chinese individuals, we found no association between GSTM1 genetic polymorphisms and lung cancer risk (OR = 1.13, 95%CI = 0.97-1.32, P = 0.13). In conclusion, we found that GSTM1 polymorphisms are not associated with lung cancer risk.

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