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Yin Y.,Ocean University of China | Yin Y.,Shanghai Ocean University | Guo J.,Shanghai Ocean University | Guo J.,First Institute of Oceanography | And 5 more authors.
5th International Conference on Bioinformatics and Biomedical Engineering, iCBBE 2011 | Year: 2011

Four strains of fungi isolated from the sediments of badly contaminated Li-Cun River were studied to investigate their ability to remove 6 target PCBs. Having different viability at different conditions, these 4 fungi all showed relatively better PCB degradation capability and higher efficiency for total PCB removal in the absence of the ligninolytic enzymes. The findings that chloride concentration changes and some acids are generated as the metabolic products contribute to our understanding of PCBs degradation pathways. Combinations of different PCB concentrations and fresh fungi weight inoculated were attempted to achieve maximum efficiency. After the bio-treatment, the concentrations of PCBs are significantly reduced with the chloride ion concentration increasing slightly. Not only as a group but also as a unit these 4 fungi show high capability in the PCBs degradation process. © 2011 IEEE.

Xie H.,Qingdao Quality Supervision and Inspection Center for Shopping Basket Commodity | Zhou M.,Chinese Academy of Fishery Sciences | Zhao H.,Qingdao Quality Supervision and Inspection Center for Shopping Basket Commodity | Wang Y.,Qingdao Quality Supervision and Inspection Center for Shopping Basket Commodity | And 2 more authors.
Chinese Journal of Chromatography (Se Pu) | Year: 2014

This study was aimed to the establishment of an analytical method for the determina tion of three exogenous plant hormone residues in bean sprout by high performance liquid chromatography-quadrupole-time of flight mass spectrometry (HPLC-Q-TOF-MS). The target compounds were gibberellins, 6-benzylaminopurine and parachlorophenoxyacetic acid. The QuECh-ERS (quick, easy, cheap, effective, rugged, and safe) method was used for sample preparation. The analytes were extracted with a solution containing 1% (v/v, if not specified) acetic, 50% ethanol, 49% acetonitrile, and cleaned-up by dispersive solid-phase extraction with diatomite dispersant, then degreased by hexane. The three target compounds were separated on an Eclipse Plus C18 column (100 mm×3. 0 mm, 1. 8 μm) with mobile phases A (water containing 0. 1% formic acid) and B (methanol) by gradient elution within 15 min, and detected under negative electrospray ionization (ESI) mode. The quantitative analysis was carried out by extracting the peak area with accurate mass. The confirmatory analysis of the target compounds was performed with the qualitative fragments. The results showed that the limits of quantification (LOQs, S/N =10) for the three target compounds were from 5. 0 μg/kg to 10 μg/kg. The respective mean recoveries were found to be in the range of 79. 1% -96. 1%, and the RSDs were 5. 7%-10. 4%. It was applicable to the analysis of the three exogenous plant hormones in bean sprout samples. This method is simple, fast and efficient.

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