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Liu M.-L.,Ocean University of China | Jiang M.,Qingdao Municipal Medical Group | Zheng K.,Ocean University of China | Li Y.-T.,Ocean University of China | And 2 more authors.
Journal of Coordination Chemistry | Year: 2014

A new mononuclear copper(II) complex identified as [Cu(bpy) 2(pic)](pic), where bpy represents 2,2-bipyridine and pic stands for picrate (pic), has been synthesized and characterized by elemental analysis, molar conductivity, IR and electronic spectral studies, and single-crystal X-ray diffraction. The crystal structure analysis reveals that copper(II) has a distorted square-pyramidal coordination geometry. The hydrogen bonding and π-π stacking interactions contribute to a 3-D supramolecular structure in the crystal. The DNA-binding properties of the copper(II) complex are investigated both theoretically and experimentally, revealing that the copper(II) complex interacts with herring sperm DNA (HS-DNA) by intercalation, and the molecular docking of the copper(II) complex with the self-complementary DNA duplex of sequence d(ACCGACGTCGGT)2 facilitates the binding events. The in vitro anticancer activities suggest that the copper(II) complex is active against selected tumor cell lines. The influence of the complexation of pic in the mononuclear copper(II) complexes on DNA-binding properties and in vitro anticancer activities is discussed. © 2014 Taylor & Francis. Source


Xing T.-T.,Ocean University of China | Zhan S.-H.,Qingdao Municipal Medical Group | Li Y.-T.,Ocean University of China | Wu Z.-Y.,Ocean University of China | Yan C.-W.,Ocean University of China
Journal of Coordination Chemistry | Year: 2013

A new tetracopper(II) complex bridged by oxamido and carboxylate, [Cu4(bhyox)2(phen)2(H2O)2](pic)2, where H3bhyox, phen and Hpic denote N-benzoate-N′-[2-(2-hydroxyethylamino)ethyl]oxamide, 1,10-phenanthroline, and 2,4,6-trinitrophenol, respectively, has been synthesized and characterized by elemental analysis, molar conductivity, IR, electronic spectra, and single-crystal X-ray diffraction The crystal structure reveals that the cyclic tetracopper(II) cation [Cu4(bhyox)2(phen)2(H2O)2]2+ with an embedded center of inversion is assembled by a pair of cis-oxamido-bridged bicopper(II) units via carboxylate bridges, in which copper(II) ions are distorted square pyramidal The CuCu separations through the oxamido and the carboxylate bridges are 5.1944(6) and 5.3344(7) Å, respectively In the crystal, the supramolecular structure is composed of classical hydrogen bonding chains assembled by 2D non-classical hydrogen-bonding networks and π-π stacking interaction In vitro cytotoxicity experiment shows that the tetracopper(II) complex exhibits cytotoxic activity against the SMMC7721 and A549 cell lines The reactivity towards herring sperm DNA (HS-DNA) and protein bovine serum albumin (BSA) suggests that the tetracopper(II) complex can interact with the DNA by intercalation, and the complex binds to protein BSA responsible for quenching of tryptophan fluorescence by static quenching mechanism © 2013 Taylor & Francis. Source


Li X.-J.,Ocean University of China | Zheng K.,Ocean University of China | Li Y.-T.,Ocean University of China | Yan C.-W.,Ocean University of China | And 2 more authors.
Journal of Coordination Chemistry | Year: 2015

A 1-D copper(II) coordination polymer formulated as {[Cu2(bdpox)(dabt)](NO3)•H2O}n, where H3bdpox and dabt denote N-benzoate-N'-[3-(diethylamino)propyl]oxamide and 2,2'-diamino-4,4'-bithiazole, respectively, was synthesized and characterized by elemental analyses, molar conductance measurement, IR and electronic spectra studies, and single-crystal X-ray diffraction. The crystal structure analysis reveals that copper(II) ions are bridged by both cis-oxamido and carboxylato groups to form a 1-D coordination polymer with corresponding Cu•••Cu separations of 5.2420(10) and 5.1551(8) Å. The endo- and exo-copper(II) ions of the cis-oxamido-bridge are located in distorted square-planar and square-pyramidal geometries, respectively. There is a 2-D hydrogen bonding network in the crystal. The in vitro anticancer activities suggest that the copper(II) complex is active against selected tumor cell lines. The reactivities toward herring sperm DNA and bovine serum albumin (BSA) reveal that the copper(II) complex can interact with DNA by intercalation and effectively quench the intrinsic fluorescence of BSA via a static mechanism. The influence of hydrophobicity of the substituents in bridging ligands on DNA and protein binding properties and the in vitro anticancer activities of such copper(II) polymers is discussed. © 2015 Taylor & Francis. Source


Jiang Q.,Capital Medical University | Yin X.,Qingdao Municipal Medical Group | Shan Z.,Capital Medical University | Ma Y.,Capital Medical University | And 3 more authors.
Molecular and Cellular Biochemistry | Year: 2013

Mesenchymal stem cells (MSCs) derived from dental tissues show promise for use in tooth-related tissue regeneration, but the molecular mechanisms underlying their directed differentiation remain unclear, limiting their usefulness. Sonic Hedgehog (Shh) signaling is a major signaling pathway that regulates cell differentiation and osteogenesis. We found that when Shh signaling was activated by human recombinant SHH-N protein or by overexpression of active mutant M2-Smoothened (SMO) in stem cells from apical papilla (SCAPs), GLI1, a key downstream transcription factor and a marker of Shh signaling, was upregulated. Subsequently, in vitro osteo/dentinogenic differentiation and in vivo osteogenesis were inhibited in SCAPs. Moreover, the expression of GLI1 and SMO were downregulated by BMP signaling while osteo/dentinogenic differentiation in SCAPs was upregulated. These results provide insights into the role of Shh signaling in the directed differentiation of MSCs derived from dental tissues and suggest possible target genes for optimizing the use of stem cells of dental origin for tissue regeneration applications. © 2013 Springer Science+Business Media New York. Source


Jiao J.,Ocean University of China | Jiang M.,Qingdao Municipal Medical Group | Li Y.-T.,Ocean University of China | Wu Z.-Y.,Ocean University of China | Yan C.-W.,Ocean University of China
Journal of Biochemical and Molecular Toxicology | Year: 2014

A new dinuclear copper(II) complex bridged by N-[3-(dimethylamino)propyl]-N′- (2-carbo-xylatophenyl)oxamide (H3dmapob), and endcapped with 2,2′-diamino-4,4′-bithiazole (dabt), namely [Cu2(dmapob)(dabt)(CH3OH)(pic)]·(DMF)0.75·(CH3OH)0.25 has been synthesized and characterized by elemental analysis, molar conductivity measurement, infrared and electronic spectra studies, and single-crystal X-ray diffraction. In the crystal structure, both copper(II) ions have square-pyramidal coordination geometries. The Cu···Cu separation through the oxamido bridge is 5.176(9) Å. A two-dimensional supramolecular framework is formed through hydrogen bonds and π-π stacking interactions. The reactivities toward herring sperm DNA and bovine serum albumin (BSA) show that the complex can interact with the DNA via intercalation mode and bind to the BSA responsible for quenching of tryptophan fluorescence by the static quenching mechanism. The in vitro anticancer activities suggest that the copper(II) complex is active against the selected tumor cell lines. The influence of different bridging ligands in dinuclear complexes on the DNA- and BSA-binding properties as well as anticancer activities is preliminarily discussed. © 2014 Wiley Periodicals, Inc. 28 2 February 2014 10.1002/jbt.21535 Research Article Research Articles © 2013 Wiley Periodicals, Inc. Source

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