Qingdao Institute for Drug Control

Qingdao, China

Qingdao Institute for Drug Control

Qingdao, China
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Chu H.,Qingdao University | Xia J.,Qingdao University | Yang Z.,Qingdao Institute for Drug Control | Gao J.,Qingdao University
International Journal of Neuroscience | Year: 2012

Melanocortin 4 receptor (MC4R) is implicated in the initiation and maintenance of neuropathic pain. Although the effect of MC4R on neuropathic pain is known, it remains unclear how MC4R mediates neuropathic pain. In vitro MC4R activates mitogen-activated kinase (MAPK). Accordingly, we investigate whether MC4R activates the p38MAPK cascade in vivo to trigger pain behavior of Wistar rats after chronic constriction injury (CCI). Intrathecal injection of MC4R antagonist HS014 (5 μg/day) at the moment of CCI for seven days attenuated thermal hyperalgesia and mechanical allodynia. Similarly, intrathecal injection of a p38 inhibitor (SB203580, 10 mg/day) at the moment of CCI for seven days was also effective. To assess whether the effects of HS014 were mediated via increased p38MAPK activation, ipsilateral L4 and L5 dorsal root ganglion (DRG) were analyzed for MC4R and phosphorylated p38MAPK (p-p38MAPK) after CCI alone or CCI combined with HS014 treatment or SB203580 treatment. After CCI, DRG p-p38MAPK and MC4R were elevated by three, seven, and 14 days. Treatment with SB203580 blocked p38 activation. Both MC4R and phosphorylated p38 localized in DRG neurons. These data suggest a sequential role for MC4R and p38 in the induction and maintenance of neuropathic pain. MC4R plays an important role in the establishment of neuropathic pain following CCI, seemingly dependent on p38 activation. © 2012 Informa Healthcare USA, Inc.


Chu H.,Qingdao University | Niu Z.,Qingdao University | Yang Z.,Qingdao Institute for Drug Control | Zhang X.,Qingdao University
Neural Regeneration Research | Year: 2010

Previous studies have confirmed that the number of glial fibrillary acidic protein-positive cells significantly increases during morphine tolerance. However, morphine tolerance is reversed with melanocortin receptor antagonists, and analgesic action is enhanced accordingly. However, these mechanisms remain unclear. In the present study, following addition of morphine to Wistar rat spinal cord astrocytes, glutamate levels in the supernatant significantly increased (P < 0.05). At 30-120 minutes following addition of intervention agent to spinal cord astrocytes, naloxone significantly increased glutamate release in morphine-tolerant model cells (P < 0.05), while melanocortin receptor antagonist HS014 decreased glutamate release (P < 0.05). Additional naloxone and HS014 to astrocytes significantly decreased glutamate release compared with additional naloxone alone (P < 0.01). Results from the present study demonstrated that glutamate release was increased in spinal cord astrocytes co-cultured with morphine. Naloxone increased glutamate release, and HS014 reduced glutamate release.


Lu J.-G.,Qingdao Institute for Drug Control | Wang L.-Y.,Qingdao Institute for Drug Control | Li X.-R.,Qingdao Institute for Drug Control | Cong J.,Qingdao Institute for Drug Control
Chinese Pharmaceutical Journal | Year: 2012

OBJECTIVE: To establish a near infrared spectroscopy method for the determination of the concentration of tiopronin for injection. METHODS: By using optical fiber probes and 1 mm sampling accessories, the near infrared transmission reflectance spectra of tiopronin for injection from seven pharmaceutical factories were obtained. The partial least square method was applied to establish the model with HPLC as the reference method. RESULTS: The model had a good prediction performance. The value of the correlation coefficient (r 2) was 99.0%, the mean difference of prediction for test sets was 1.30%, and the root mean square error of prediction (RMSEP) was 0.982%. CONCLUSION: NIR is a rapid, reliable and powerful method for the quantitation of tiopronin for injection, and can be applied to rapid drug determination on the spot and stability study. Copyright 2012 by the Chinese Pharmaceutical Association.


Fan Y.,The Qingdao First Sanatorium of PLA | Yang Z.,Qingdao Institute for Drug Control | Zhu Y.-J.,Qingdao Institute for Drug Control
Chinese Pharmaceutical Journal | Year: 2013

OBJECTIVE: To establish a method for determination of four nitrofuran metabolites in animal-derived heparin by UPLC triple-quadruple tandem mass spectrometer. METHODS: Samples are hydrolyzed with hydrochloric acid, and derivatized at 37°C for 16 h with 2-nitrobenzaldehyde. The target compounds are eluted with ethyl acetate. Identification is achieved by electrospray ionization in positive mode (ESI+) using multiple reaction monitoring (MRM). The quantification is performed with internal standards. RESULTS: A linearity of nitrofuran antibiotics is obtained from 0.1 to 10.0 μg · L-1. r are 0.994-0.996. The LODs are between 0.08-0.226 μg · kg-1 and the average recoveries are 82.4%-109.7% respectively. CONCLUSION: The method is rapid, precise, sensitive, and suitable for the determination of four nitrofuran metabolites in heparin.


Sun L.,National Institutes for Food and Drug Control | Zhang C.,Suzhou Institute for Food and Drug Control | Tian R.-T.,Chemmind Technologies Co. | Liu L.-N.,National Institutes for Food and Drug Control | And 3 more authors.
Chinese Pharmaceutical Journal | Year: 2015

OBJECTIVE: To propose and establish a novel holistic strategy of identification and quality evaluation of frankincense by chromatographic fingerprint analysis. METHODS: The strategy contained five steps. The first step was multi-species sample collection. The second was to establish fingerprint identification and assay under the same chromatographic conditions. The third was components identification by means of reference substances and LC-MS. The fourth was multivariate analysis for identification and classification of certified products and adulterants. The fifth was to evaluate TCMs using similarity threshold and content range criteria. RESULTS: Twenty-six chromatographic peaks were separated and fourteen were identified. Furthermore, HCA heatmap analysis, principal component analysis, partial least squares discrimination, self-organizing map clustering, and support vector machine were used for pattern recognition. The common patterns for three species of frankincenses were established. The content ranges for KBA and AKBA were also calculated. CONCLUSION: The method is specific and can be used to accurately identify and systematically evaluate medicinal frankincense. ©, 2015, Chinese Pharmaceutical Association. All right reserved.


Zhong N.,QingDao Institute for Drug Control | Zhong L.,ShanDong City Service Technology | Hao L.,First Institute of Oceanography | Luan C.,QingDao Institute for Drug Control | Li X.,QingDao Institute for Drug Control
Analytical Letters | Year: 2015

Selenium species in enriched garlic (Allium Sativum L) sprouts were determined by high-performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The garlic was grown in a 10 mg/L selenite nutrient solution, and the total selenium accumulated by the sprouts was 250 µg/g. Three mobile phase systems were investigated and a buffer of 20 mmol/L ammonium acetate/5% methanol was chosen for subsequent analysis. Comparative experiments were performed to determine the selenium species in the garlic sprouts with four extraction solutions: 0.1 mol/L HCl, 0.1 mol/L NaOH, 20 mmol/L ammonium acetate/5% methanol and protease XIV. The most suitable results were obtained using 0.1 mol/L HCl as the extracting solution. Validation was performed; all selenium compounds had recoveries of 102.5% to total selenium, with good linearity and precision. The major compound accumulated in the garlic sprouts was methylselenocysteine, which accounted for 65.01% of the selenium. © 2015, Copyright Taylor & Francis Group, LLC.


Wang X.,Qingdao University | Wang X.,Qingdao Institute for Drug Control | Jiang Q.,Qingdao University | Wang W.,Qingdao University | And 3 more authors.
Journal of Photochemistry and Photobiology B: Biology | Year: 2014

This study investigated the molecular mechanisms of polypeptides from Chlamys farreri (PCF)'s anti-apoptotic effects in ultraviolet A-rays (UVA) exposed HaCaT cells. UVA-induced apoptosis in HaCaT cells was confirmed with Hoechst 33258 fluorescent staining; PCF treatment inhibited UVA-induced apoptosis in HaCaT cells, increased transcriptional activities of heat shock factor protein 1 (HSF1) and the expression of heat shock protein 70 (HSP70), whereas inhibited activation of c-Jun N-terminal kinases (JNK), expression of xanthine oxidese (XO), inducible nitric oxide synthase (iNOS) and release of nitric oxide (NO)/reactive oxygen species (ROS). Meanwhile, the HSF1 transcription inhibitor quercetin increased UVA-induced apoptosis, activation of JNK, expression of XO and iNOS and release of NO/ROS. Among the two NO release peaks we found in UVA exposed HaCaT cells, XO inhibitor oxypurinol was found to be able to inhibit NO release at 3 h post UVA exposure but not 18 h, while iNOS inhibitor S-methylisothiourea sulfate (SMT) was found to inhibit iNOS expression and NO release at 18 h but not 3 h. PCF's protection against UVA-induced apoptosis in HaCaT cells involves increased transcriptional activity of HSF1, increased expression of HSP70, and the subsequential inhibition of JNK pathway, XO and iNOS expression and ROS/NO release. © 2013 Elsevier B.V. All rights reserved.


Hao L.,State Oceanic Administration | Chen L.,Qingdao Municipal Hospital | Chen L.,Shandong University | Hao J.,Qingdao Technological University | Zhong N.,Qingdao Institute for Drug Control
Ecotoxicology and Environmental Safety | Year: 2013

In this study, bioaccumulation and sub-acute toxicity of water-borne nano-ZnO in the test fish, juvenvile carp (Cyprinus Carpio) were evaluated. To clarify the contribution of particle size and free Zn ion to NPs toxicity, its bulk counterparts (bulk-ZnO) and the released Zn2+ were also tested. The results showed that after a 30-day exposure, 50mg/L of nano-ZnO and bulk-ZnO could be significantly accumulated and distributed in various tissues of fish, but nano-ZnO exhibited more hyper-bioaccumulation than bulk-ZnO. Liver and gill might be the target tissues with exposure to nano-ZnO, instead, the target tissue for bulk-ZnO might be intestine. Also, 50mg/L of nano-ZnO caused more severe histopathological changes than the same concentration of bulk-ZnO, which was in accordance with the induction of higher levels of intracellular oxidative stress. The effects of dissolved Zn ions were assessed and the ion toxicity was negligible herein. The results of this study indicated that the observed toxicities of nano-ZnO were not likely a result solely of particle dissolution and identified as a function of particle toxicity and the possibility for a size dependence. The main toxic mechanism of nano-ZnO was possibly by increasing cellular oxidative stress response. © 2013 Elsevier Inc.


PubMed | Qingdao Institute for Drug Control
Type: Journal Article | Journal: Se pu = Chinese journal of chromatography | Year: 2010

An analytical method for the solvent residues in the raw material drug of glimepiride has been established by head-space sampling capillary gas chromatography. General evaluation was made for the distribution of organic residual solvents in glimepiride samples from 8 different domestic manufacturers. Based on the evaluation-test results and the information provided by manufacturers, 14 target solvents were ascertained including acetone, ethyl acetate, methanol, isopropanol, ethanol, chloroform, toluene, 1,4-dioxane, pyridine, chlorobenzene, ether, dichloromethane, n-hexane and benzene. The target solvents were divided into two groups for baseline separation according to their column-retention specificity. Acetone, ethyl acetate, methanol, isopropanol, ethanol, chloroform, toluene, 1,4-dioxane, pyridine and chlorobenzene were separated on a Supelco-Wax capillary column with acetonitrile as internal standard, while ether, dichloromethane, n-hexane and benzene were determined on a Supelco OVI-G43 capillary column with butanone as internal standard. Linear responses were obtained for the 14 residual solvents in their respective concentration ranges (r = 0.99167-0.99997, n = 8), and the limits of detection were 0.2-13.5 microg/g. The inter-day reproducibilities, measured as relative standard deviations (RSDs), were 0.6%-9.2% (n = 3). The average recoveries of three concentration levels were 86.3%-104.1% with the RSD of 0.2%-5.3% (n = 16). The developed method is simple, sensitive, and accurate for the residual solvent analysis in glimepiride samples.


PubMed | Qingdao Institute for Drug Control, Qingdao Hiser Medical Center and TCM Hospital of Zhangdian District of Zibo City
Type: Journal Article | Journal: Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2014

To observe the clinical efficacy and safety of electroacupuncture (EA) combined with herbal acupoint sticking in the treatment of Bells palsy and provide optimizations for the clinic.One hundred and two cases of Bells palsy were randomized into an EA combined with herbal acupoint sticking group (group A, 50 cases) and an EA group (group B, 52 cases), EA at Cuanzhu (BL 2), Yangbai (GB 14), Taiyang (EX-HN 5), Quanliao (SI 18),Xiaguan (ST 7), Yingxiang (LI 20), etc. were applied in both groups and facial paralys No.I was applied at Yifeng (TE 17) in group A, once daily and 10 times totally were needed. The score of facial nerve function, clinical efficacy were compared before and after treatment. At 1 and 3 month follow up visit, the quality of life scale( WHOQOL-BREF) and the occurrence of complication were observed.The scores of facial nerve function in group A and group B were all significantly improved compared with those before treatment (48. 2+/- 2. 9 vs 25. 7 +/- 4. 9, 45. 9 +/- 6. 2 vs 25. 8 +/- 5. 5, both P0. 05). The occurrence of complication in group A (1 case) was significantly less than that in group B (8 cases, P 0. 05).Compared with EA, the combination of EA and acupoint sticking therapy for Bells palsy cannot only improve the clinical efficacy and reduce the occurrence of complication but also reliable without any side effect.

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