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Hao L.,State Oceanic Administration | Chen L.,Qingdao Municipal Hospital | Chen L.,Shandong University | Hao J.,Qingdao Technological University | Zhong N.,Qingdao Institute for Drug Control
Ecotoxicology and Environmental Safety | Year: 2013

In this study, bioaccumulation and sub-acute toxicity of water-borne nano-ZnO in the test fish, juvenvile carp (Cyprinus Carpio) were evaluated. To clarify the contribution of particle size and free Zn ion to NPs toxicity, its bulk counterparts (bulk-ZnO) and the released Zn2+ were also tested. The results showed that after a 30-day exposure, 50mg/L of nano-ZnO and bulk-ZnO could be significantly accumulated and distributed in various tissues of fish, but nano-ZnO exhibited more hyper-bioaccumulation than bulk-ZnO. Liver and gill might be the target tissues with exposure to nano-ZnO, instead, the target tissue for bulk-ZnO might be intestine. Also, 50mg/L of nano-ZnO caused more severe histopathological changes than the same concentration of bulk-ZnO, which was in accordance with the induction of higher levels of intracellular oxidative stress. The effects of dissolved Zn ions were assessed and the ion toxicity was negligible herein. The results of this study indicated that the observed toxicities of nano-ZnO were not likely a result solely of particle dissolution and identified as a function of particle toxicity and the possibility for a size dependence. The main toxic mechanism of nano-ZnO was possibly by increasing cellular oxidative stress response. © 2013 Elsevier Inc. Source


Zhang Y.,Qingdao Institute for Drug Control | Yang Z.,Qingdao Institute for Drug Control | Zhu R.-J.,Qingdao University | Li X.-R.,Qingdao Institute for Drug Control | And 2 more authors.
Chinese Pharmaceutical Journal | Year: 2012

OBJECTIVE: To establish a method for determination of 30 antihypertensive medicines adulterated in traditional Chinese patent medicines and health foods by UPLC triple-quadrupole tandem mass spectrometer. METHODS: Multi-reactions monitoring (MRM) technology was used. The separation was performed on a Waters ACQUTTY UPLC™ BEHC 18 column with a gradient mobile phase of methanol - 0.1% formic acid at flow rate of 0.2 mL · min -1. The ionization mode was ESI +. RESULTS: The standard curves of the 30 chemical medicines showed good linearity over the concentration ranges of 10 - 1000.0, 0 - 400.0 and 2.0 - 400.0 μg · L -1 (r = 0.9903-0.9998). The detection limits of the 30 chemical medicines were between 0.01 - 0.83 μg · L -1 and the average recoveries were 72.2% - 123.4% at 3 levels. CONCLUSION: The method is rapid and sensitive, and suitable for the determination of 30 chemical medicines in traditional Chinese patent medicines and health foods. Copyright 2012 by the Chinese Pharmaceutical Association. Source


Wang X.,Qingdao University | Wang X.,Qingdao Institute for Drug Control | Jiang Q.,Qingdao University | Wang W.,Qingdao University | And 3 more authors.
Journal of Photochemistry and Photobiology B: Biology | Year: 2014

This study investigated the molecular mechanisms of polypeptides from Chlamys farreri (PCF)'s anti-apoptotic effects in ultraviolet A-rays (UVA) exposed HaCaT cells. UVA-induced apoptosis in HaCaT cells was confirmed with Hoechst 33258 fluorescent staining; PCF treatment inhibited UVA-induced apoptosis in HaCaT cells, increased transcriptional activities of heat shock factor protein 1 (HSF1) and the expression of heat shock protein 70 (HSP70), whereas inhibited activation of c-Jun N-terminal kinases (JNK), expression of xanthine oxidese (XO), inducible nitric oxide synthase (iNOS) and release of nitric oxide (NO)/reactive oxygen species (ROS). Meanwhile, the HSF1 transcription inhibitor quercetin increased UVA-induced apoptosis, activation of JNK, expression of XO and iNOS and release of NO/ROS. Among the two NO release peaks we found in UVA exposed HaCaT cells, XO inhibitor oxypurinol was found to be able to inhibit NO release at 3 h post UVA exposure but not 18 h, while iNOS inhibitor S-methylisothiourea sulfate (SMT) was found to inhibit iNOS expression and NO release at 18 h but not 3 h. PCF's protection against UVA-induced apoptosis in HaCaT cells involves increased transcriptional activity of HSF1, increased expression of HSP70, and the subsequential inhibition of JNK pathway, XO and iNOS expression and ROS/NO release. © 2013 Elsevier B.V. All rights reserved. Source


Zhong N.,Qingdao Institute for Drug Control | Zhong L.,ShanDong City Service Technology | Hao L.,First Institute of Oceanography | Luan C.,Qingdao Institute for Drug Control | Li X.,Qingdao Institute for Drug Control
Analytical Letters | Year: 2015

Selenium species in enriched garlic (Allium Sativum L) sprouts were determined by high-performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The garlic was grown in a 10 mg/L selenite nutrient solution, and the total selenium accumulated by the sprouts was 250 µg/g. Three mobile phase systems were investigated and a buffer of 20 mmol/L ammonium acetate/5% methanol was chosen for subsequent analysis. Comparative experiments were performed to determine the selenium species in the garlic sprouts with four extraction solutions: 0.1 mol/L HCl, 0.1 mol/L NaOH, 20 mmol/L ammonium acetate/5% methanol and protease XIV. The most suitable results were obtained using 0.1 mol/L HCl as the extracting solution. Validation was performed; all selenium compounds had recoveries of 102.5% to total selenium, with good linearity and precision. The major compound accumulated in the garlic sprouts was methylselenocysteine, which accounted for 65.01% of the selenium. © 2015, Copyright Taylor & Francis Group, LLC. Source


Qi Q.H.,Qingdao Hiser Medical Center | Ni S.S.,Qingdao Hiser Medical Center | Wang Y.L.,Qingdao Institute for Drug Control | Peng K.,TCM Hospital of Zhangdian District of Zibo City | And 4 more authors.
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2013

To observe the clinical efficacy and safety of electroacupuncture (EA) combined with herbal acupoint sticking in the treatment of Bell's palsy and provide optimizations for the clinic. One hundred and two cases of Bell's palsy were randomized into an EA combined with herbal acupoint sticking group (group A, 50 cases) and an EA group (group B, 52 cases), EA at Cuanzhu (BL 2), Yangbai (GB 14), Taiyang (EX-HN 5), Quanliao (SI 18),Xiaguan (ST 7), Yingxiang (LI 20), etc. were applied in both groups and "facial paralys No.I " was applied at Yifeng (TE 17) in group A, once daily and 10 times totally were needed. The score of facial nerve function, clinical efficacy were compared before and after treatment. At 1 and 3 month follow up visit, the quality of life scale( WHOQOL-BREF) and the occurrence of complication were observed. The scores of facial nerve function in group A and group B were all significantly improved compared with those before treatment (48. 2+/- 2. 9 vs 25. 7 +/- 4. 9, 45. 9 +/- 6. 2 vs 25. 8 +/- 5. 5, both P0. 05). The occurrence of complication in group A (1 case) was significantly less than that in group B (8 cases, P 0. 05). Compared with EA, the combination of EA and acupoint sticking therapy for Bell's palsy cannot only improve the clinical efficacy and reduce the occurrence of complication but also reliable without any side effect. Source

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