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Qu S.-Z.,Qingdao University | Li S.-Z.,Qingdao University | Gao J.-K.,Qingdao University | Zhang J.-F.,Qingdao Hiser Medical Group
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Osteosarcoma is the most common bone primary malignant tumors, and the occurrence of osteosarcoma may relate with osteosarcoma stem cells. OBJECTIVE: To evaluate the isolation, culture and identification method of osteosarcoma stem cells, to investigate the expression of relative tumor markers. METHODS: The osteosarcoma stem cells were isolated and cultured with magnetic activated cell sorting method under serum-free condition and serum-free joint antineoplastic condition, in order to sort Stro-1 positive and CD133 positive osteosarcoma stem cells. The expression level and tumorigenic properties of CD133, Oct3 / 4 and Nanog markers of osteosarcoma cancer stem cells were tested with immunofluorescence staining and Western blotting methods. RESULTS AND CONCLUSION: Suspended cell condensation could be seen in the osteosarcoma stem cells after culture for 2-10 days, the proliferation incubation period was about 24 hours, and the Stro-1 positive stem cells could form the suspended cell condensation, while the Stro-1 negative stem cells could not form the suspended cell condensation. In addition, osteosarcoma stem cells could highly express Oct3/4, Nanog and CD133, the CD133-positive osteosarcoma stem cells could highly express CD133 molecule with strong invasiveness, while the CD133 negative cells could not express CD133 molecule and the invasiveness was weak. Celecoxib could inhibit the formation of osteosarcoma stem cells to some extent, and could reduce the expression of tumor angiogenic blood vessels and vascular endothelial growth factor. Source

Lin R.,Qingdao University | Guan R.,Qingdao University | Liu X.,Qingdao University | Zhao B.,Qingdao University | And 2 more authors.
BMC Public Health | Year: 2014

Conclusions: The prevalence of asthma in children aged 0-14 years in Qingdao China increased significantly based on data obtained ten years ago (2000). Respiratory tract infections were the most important precursors of asthma attack. The attack was most commonly manifested as cough. The treatment, especially the use of ICS, was more rational. However, a certain difference was found, which has yet to be contrasted with the Global Initiative for Asthma (GINA) project.Background: Recent investigations suggested that the trend of childhood asthma has been stabilizing or even reversing in some countries. The observation provides contrast to our experience. Thus, the study aimed to investigate the prevalence and clinical features of asthma in children aged 0-14 years in Qingdao China, determine the changes of childhood asthma in China, and discover evidence that can allow better diagnosis and treatment of childhood asthma.Methods. A cluster sampling method was used. We randomly extracted the investigation clusters from schools, kindergartens, and communities in Qingdao. Subsequently, we interviewed the members of the clusters using a questionnaire from the International Study of Asthma and Allergies in Childhood (ISAAC) to find children with asthmatic symptoms. After determination by the doctors, more details on the asthmatic children were obtained by asking questions from the National Epidemiology Study of Asthma and Allergies in China questionnaire to obtain more details. We intended to survey 10,800 children. However, the actual number of children was 10,082.Results: The prevalence of asthma in Qingdao children aged 0-14 years was 3.69%. The prevalence among male children was higher than in female (χ2 = 24.53,P < 0.01). Among the asthmatic children, 68.0% had their first attack when they were less than three years old. Moreover, 71.2% once suffered respiratory tract infections. For 95.7% of asthmatic children, the asthma attack was first manifested as cough. Asthmatic children who used inhaled corticosteroids (ICS) only accounted for 46%. © 2014Lin et al.; licensee BioMed Central Ltd. Source

Liang H.,Harbin Medical University | Guan D.,Qingdao Hiser Medical Group | Gao A.,Northeast Agricultural University | Yin Y.,Harbin Medical University | And 5 more authors.
Cytotherapy | Year: 2014

Background aims: The molecular mechanisms by which stem cell transplantation improves functional recovery after intracerebral hemorrhage (ICH) are not well understood. Accumulating evidence suggests that microglia cells are activated shortly after ICH and that this activation contributes to secondary ICH-induced brain injury. We studied the effect of human amniotic epithelial stem cells (HAESCs) on microglia activation. Methods: To study the effect of HAESCs in vitro, we used thrombin to activate the microglia cells. Twenty-four hours after thrombin treatment, the levels of tumor necrosis factor-α and interleukin-1β were measured by enzyme-linked immunosorbent assay. In vivo, the HAESCs were transplanted into the rat striatum 1 day after collagenase-induced ICH. The expression levels of matrix metalloproteinase (MMP)-12 and microglia infiltration in the peri-hematoma tissues were determined 7 days after ICH through the use of reverse transcriptase-polymerase chain reaction and immunohistochemical analysis, respectively. Results: Thrombin-activated microglia expression of tumor necrosis factor-α, interleukin-1β and MMP-12 was significantly reduced through contact-dependent and paracrine mechanisms when the HAESCs were co-cultured with microglia cells. After transplantation of HAESCs in rat brains, the expression levels of MMP-12 and microglia infiltration in the peri-hematoma tissues were significantly reduced. Conclusions: Our observations suggest that microglia activation could be inhibited by HAESCs both in vitro and in vivo, which may be an important mechanism by which the transplantation of HAESCs reduces brain edema and ameliorates the neurologic deficits after ICH. Therefore, we hypothesize that methods for suppressing the activation of microglia and reducing the inflammatory response can be used for designing effective treatment strategies for ICH. © 2014 International Society for Cellular Therapy. Source

Liu Q.,Shandong University | Gao F.,Qingdao Hiser Medical Group | Liu X.,Qingdao Women and Children Hospital | Li J.,Qingdao University | And 3 more authors.
Archives of Gynecology and Obstetrics | Year: 2016

Purpose: Limited studies have reported the effect of prenatal alcohol exposure (PAE) on fetal liver development or liver dysfunction. The current review was conducted to systematically review published studies of PAE and liver dysfunction. Methods: Pub Med, Embase and Web of Science database were searched using terms of “prenatal alcohol exposure” and “liver” or “fetal alcohol spectrum disease” and “liver”. The pooled effect size of alcohol exposure was assessed by Hedges’s g and 95 % confidence interval (CI) using fixed model or random model depending on the heterogeneity determined by Q test and I2 statistic. Results: A total of 23 studies were included. The results indicated that gestation alcohol exposure resulted in significant reduction of fetal body weight (Hedges’s g = −6.854 ± 1.149, 95 % CI −9.106 to −4.602, P < 0.001), but not fetal liver weight reduction (Hedges’s g = −0.076 ± 0.878, 95 % CI −1.799 to 1.647, P = 0.931). PAE resulted in significant decline in protein synthesis or enzyme activity of offspring fetal liver including glutathione and 25(OH)2D (Hedges’s g = −1.149 ± 0.108, 95 % CI −1.361 to −0.938, P < 0.001), as well as significant increase in proteins including oxidants and collagen (Hedges’s g = 1.330 ± 0.146, 95 % CI 1.044–1.616, P < 0.001). Conclusion: These results suggested that PAE affects fetal body weight but not liver weight, and that PAE may result in offspring fetal liver dysfunction. © 2016, Springer-Verlag Berlin Heidelberg. Source

Chen S.,Shandong University | Zhu Y.,Shandong University | Feng X.,Qingdao Hiser Medical Group | Zhang Z.,Shandong University | And 2 more authors.
Urologia Internationalis | Year: 2014

Results: In diabetic rats, the lowest urethral pressure (UPP nadir) during urethral relaxation was significantly higher. Intravenous administration of tamsulosin, an a1-adrenoceptor antagonist, significantly decreased the UPP nadir and baseline UPP in diabetic rats. RT-qPCR and Western blotting studies showed a statistically significant increase of a1a- and a1b-adrenoceptor in the urethras from the diabetic group (p < 0.05). The expression of NGF was significantly decreased in the urethras from the diabetic group while the expression of proNGF was significantly increased (p < 0.05). The p75 NTR level in the urethras of diabetic rats was decreased compared with controls (p < 0.05) and there was no significant difference regarding sortilin between the two groups (p > 0.05).Conclusion: This study validated the diabetic urethral dysfunction and furthermore indicated that the increase in the expression of a1-adrenoceptor and changes in the NGF/ proNGF pathway may be involved in diabetic urethral dysfunction.Objective: To investigate the changes in the a1-adrenoceptor and nerve growth factor (NGF)/NGF precursor (proNGF) pathway in the urethra after diabetes induction.Materials and Methods: Urethral relaxation function was determined by simultaneous recordings of intravesical pressure under isovolumetric conditions and urethral perfusion pressure (UPP) in diabetic rats. The expression of a1-adrenoceptor, NGF, proNGF, low-affinity p75 receptor for neurotrophins (p75 NTR ) and sortilin in the urethras was measured using realtime quantitative polymerase chain reaction (RT-qPCR), enzyme- linked immunosorbent assay and Western blotting. © 2014 S. Karger AG, Basel. Source

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