Qingdao Hiser Medical Group

Qingdao, China

Qingdao Hiser Medical Group

Qingdao, China
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Lang J.,Qingdao Hiser Medical Group | Li W.,Qingdao Hiser Medical Group | Zhao J.,Qingdao Hiser Medical Group | Wang K.,Qingdao Hiser Medical Group | Chen D.,Qingdao Hiser Medical Group
Xenobiotica | Year: 2017

1. Curculigoside possesses numerous pharmacological activities, and however, little data available for the effects of curculigoside on the activity of human liver cytochrome P450 (CYP) enzymes. 2. This study investigates the inhibitory effects of curculigoside on the main human liver CYP isoforms. In this study, the inhibitory effects of curculigoside on the eight human liver CYP isoforms 1A2, 2A6, 2E1, 2D6, 2C9, 2C19, 2C8, and 3A4 were investigated in human liver microsomes. 3. The results indicated that curculigoside could inhibit the activity of CYP1A2, CYP2C8, and CYP3A4, with IC50 values of 15.26, 11.93, and 9.47 μM, respectively, but that other CYP isoforms were not affected. Enzyme kinetic studies showed that curculigoside was not only a noncompetitive inhibitor of CYP1A2, but also a competitive inhibitor of CYP2C8 and CYP3A4, with Ki values of 5.43, 3.54, and 3.35 μM, respectively. In addition, curculigoside is a time-dependent inhibitor for CYP1A2, with kinact/KI values of 0.056/6.15 μM−1 min−1. 4. The in vitro studies of curculigoside with CYP isoforms suggest that curculigoside has the potential to cause pharmacokinetic drug interactions with other coadministered drugs metabolized by CYP1A2, CYP2C8, and CYP3A4. Further in vivo studies are needed in order to evaluate the significance of this interaction. © 2017 Informa UK Limited, trading as Taylor & Francis Group.


Zhao X.,Qingdao Hiser Medical Group | Wu Y.,Qingdao Hiser Medical Group | Wang D.,Qingdao Hiser Medical Group
European Journal of Drug Metabolism and Pharmacokinetics | Year: 2017

Background and Objectives: Pristimerin has been reported to possess a wide range of pharmacological activities. This study investigates the effects of glycyrrhizic acid on the pharmacokinetics of pristimerin in rats. Methods: The pharmacokinetics of orally administered pristimerin (2 mg/kg) with or without glycyrrhizic acid pretreatment (at a dose of 100 mg/kg/day for 7 days) were investigated. The plasma concentration of pristimerin was determined using a sensitive and reliable LC–MS/MS method, and the pharmacokinetics profiles were calculated and compared. Additionally, Caco-2 cell transwell model and rat liver microsome incubation experiments were also conducted to investigate its potential mechanism. Results: The results showed that when the rats were pretreated with glycyrrhizic acid, the maximum concentration (Cmax) of pristimerin decreased from 186.43 ± 14.18 to 124.62 ± 18.49 ng/mL, and area under the concentration–time curve from zero to infinity (AUC0–inf) also decreased from 918.54 ± 144.72 to 504.72 ± 115.63 μg·h/L. The elimination half-life (t1/2) value of pristimerin decreased from 3.16 ± 1.18 to 1.88 ± 0.76 h (P < 0.05). The Caco-2 cell transwell experiments indicated that glycyrrhizic acid could increase the efflux ratio of pristimerin from 2.39 to 3.64, and the rat liver microsome incubation experiments showed that glycyrrhizic acid could significantly increase its intrinsic clearance rate from 51.87 ± 5.34 to 76.79 ± 8.52 µL/min/mg protein. Conclusions: In conclusion, these results indicated that glycyrrhizic acid could affect the pharmacokinetics of pristimerin, and it might work through decreasing the absorption of pristimerin by inducing the activity of P-gp or through increasing the clearance rate in rat liver by inducing the activity of cytochrome P450 enzyme. © 2017 Springer International Publishing AG


PubMed | Eighth Peoples Hospital of Qingdao, Qingdao Women and Children Hospital, Qingdao Hiser Medical Group, Shandong University and Qingdao University
Type: Journal Article | Journal: Archives of gynecology and obstetrics | Year: 2016

Limited studies have reported the effect of prenatal alcohol exposure (PAE) on fetal liver development or liver dysfunction. The current review was conducted to systematically review published studies of PAE and liver dysfunction.Pub Med, Embase and Web of Science database were searched using terms of prenatal alcohol exposure and liver or fetal alcohol spectrum disease and liver. The pooled effect size of alcohol exposure was assessed by Hedgess g and 95% confidence interval (CI) using fixed model or random model depending on the heterogeneity determined by Q test and I (2) statistic.A total of 23 studies were included. The results indicated that gestation alcohol exposure resulted in significant reduction of fetal body weight (Hedgess g=-6.8541.149, 95% CI -9.106 to -4.602, P<0.001), but not fetal liver weight reduction (Hedgess g=-0.0760.878, 95% CI -1.799 to 1.647, P=0.931). PAE resulted in significant decline in protein synthesis or enzyme activity of offspring fetal liver including glutathione and 25(OH)2D (Hedgess g=-1.1490.108, 95% CI -1.361 to -0.938, P<0.001), as well as significant increase in proteins including oxidants and collagen (Hedgess g=1.3300.146, 95% CI 1.044-1.616, P<0.001).These results suggested that PAE affects fetal body weight but not liver weight, and that PAE may result in offspring fetal liver dysfunction.


PubMed | Shandong Provincial Hospital, Qingdao Women and Children Hospital, Qingdao Hiser Medical Group, Eighth Peoples Hospital of Qingdao and Qingdao University
Type: Journal Article | Journal: Genetics and molecular research : GMR | Year: 2016

In this case-control study, we assessed the influence of IL-10 -1082A/G and -819T/C on the development of preeclampsia. The IL-10 -1082A/G and -819T/C polymorphisms were assessed by polymerase chain reaction-restriction fragment length polymorphism. The genotype distributions of the IL-10 -1082A/G and -819T/C polymorphisms in the control subjects were in conformance with Hardy-Weinberg equilibrium (HWE; P = 0.46 and 0.17). Unconditional logistic regression analyses revealed that individuals carrying the CC genotype of IL-10 -819T/C were associated with an increased risk of preeclampsia compared to the TT genotype. The odds ratio (95% confidence interval) for the CC genotype of IL-10 -819T/C was 1.71 (1.07-3.27) compared to the TT genotype. In conclusion, the results of our study indicated that the IL-10 -819T/C polymorphism was associated with an increased risk of preeclampsia in a Chinese population.


Liang H.,Harbin Medical University | Guan D.,Qingdao Hiser Medical Group | Gao A.,Northeast Agricultural University | Yin Y.,Harbin Medical University | And 5 more authors.
Cytotherapy | Year: 2014

Background aims: The molecular mechanisms by which stem cell transplantation improves functional recovery after intracerebral hemorrhage (ICH) are not well understood. Accumulating evidence suggests that microglia cells are activated shortly after ICH and that this activation contributes to secondary ICH-induced brain injury. We studied the effect of human amniotic epithelial stem cells (HAESCs) on microglia activation. Methods: To study the effect of HAESCs in vitro, we used thrombin to activate the microglia cells. Twenty-four hours after thrombin treatment, the levels of tumor necrosis factor-α and interleukin-1β were measured by enzyme-linked immunosorbent assay. In vivo, the HAESCs were transplanted into the rat striatum 1 day after collagenase-induced ICH. The expression levels of matrix metalloproteinase (MMP)-12 and microglia infiltration in the peri-hematoma tissues were determined 7 days after ICH through the use of reverse transcriptase-polymerase chain reaction and immunohistochemical analysis, respectively. Results: Thrombin-activated microglia expression of tumor necrosis factor-α, interleukin-1β and MMP-12 was significantly reduced through contact-dependent and paracrine mechanisms when the HAESCs were co-cultured with microglia cells. After transplantation of HAESCs in rat brains, the expression levels of MMP-12 and microglia infiltration in the peri-hematoma tissues were significantly reduced. Conclusions: Our observations suggest that microglia activation could be inhibited by HAESCs both in vitro and in vivo, which may be an important mechanism by which the transplantation of HAESCs reduces brain edema and ameliorates the neurologic deficits after ICH. Therefore, we hypothesize that methods for suppressing the activation of microglia and reducing the inflammatory response can be used for designing effective treatment strategies for ICH. © 2014 International Society for Cellular Therapy.


Wang K.,Qingdao Hiser Medical Group | Zhao J.,Qingdao Hiser Medical Group | Lang J.,Qingdao Hiser Medical Group
Pharmaceutical Biology | Year: 2016

Context: Clarifying the potential mechanism of the poor oral bioavailability of curculigoside would be helpful for for investigating pharmacological effects and clinical applications. Objective: To clarify the main mechanism for poor oral bioavailability. Materials and methods: First, the pharmacokinetics of curculigoside (20 mg/kg) in rats with and without pretreatment with verapamil (10 mg/kg) was determined using a sensitive and reliable LC-MS method. Then the effects of verapamil on the transport and metabolic stability of curculigoside were investigated using Caco-2 cell transwell model and rat liver microsome incubation systems. Results: The results showed that verapamil could significantly increase the peak plasma concentration (from 60.17 ng/mL to 93.66 ng/mL) and AUC0−t (from 289.57 to 764.02 ng·h/mL) of curculigoside. The Caco-2 cell experiments indicated that the efflux ratio of curculigoside was 3.92 (PappAB 6.43 ± 0.57 × 10 −7 cm/s; PappBA 2.52 ± 0.37 × 10 −36 cm/s), P-gp might be involved in the transport of curculigoside, and verapamil could inhibit the efflux of curculigoside and increase the absorption of curculigoside significantly in the Caco-2 cell monolayer. Additionally, the rat liver microsome incubation experiments indicated that verapamil could significantly decrease the intrinsic clearance rate of curculigoside (from 38.8 to 23.6 μL/min/mg protein). Discussion and conclusion: These results indicated that verapamil could significantly change the pharmacokinetic profiles of curculigoside in rats, the poor absorption due to P-gp mediated efflux in intestine and high intrinsic clearance rate in rat liver may be the main reason for the poor oral absolute bioavailability of curculigoside. © 2016 Informa UK Limited, trading as Taylor & Francis Group


Zhu X.,Qingdao University of Science and Technology | Liu D.,Qingdao University of Science and Technology | Zhang Q.,Qingdao University of Science and Technology | Zhou Z.,Qingdao Hiser Medical Group | Liang W.,Qingdao Hiser Medical Group
Journal of Computers | Year: 2011

An optimal thenar palmprint classification model is proposed in this paper. Firstly, the thenar palmprint image is enhanced using a high-frequency emphasis filter and histogram equalization. Then, from the enhanced image thirteen textural features of gray level co-occurrence matrix (GLCM) are extracted as classification feature vectors. Finally, the SVM classifier is used for classification and the best classification model will be obtained through comparing the classification results of different kernel functions and feature vectors. The experimental results proved the feasibility and effectiveness of this model for thenar palmprint classification. © 2011 ACADEMY PUBLISHER.


Zhang Y.X.,Qingdao Hiser Medical Group
Chinese journal of traumatology = Zhonghua chuang shang za zhi / Chinese Medical Association | Year: 2013

Pneumocephalus is the presence of air in the cranial vault. The common etiologies of pneumocephalus are brain trauma and cranial surgery. We report a case of a 26-year-old man with brain trauma who developed diffuse pneumocephalus after sneezing. CT scan was performed on arrival, and the image showed subarachnoid hemorrhage without pneumocephalus. On the seventh day after a big sneeze brain CT scan was re-performed, which showed pneumocephalus. After another ten days of treatment, the patient was discharged without any symptoms.


PubMed | Qingdao Hiser Medical Group
Type: Journal Article | Journal: Pharmaceutical biology | Year: 2016

Clarifying the potential mechanism of the poor oral bioavailability of curculigoside would be helpful for for investigating pharmacological effects and clinical applications.To clarify the main mechanism for poor oral bioavailability.First, the pharmacokinetics of curculigoside (20mg/kg) in rats with and without pretreatment with verapamil (10mg/kg) was determined using a sensitive and reliable LC-MS method. Then the effects of verapamil on the transport and metabolic stability of curculigoside were investigated using Caco-2 cell transwell model and rat liver microsome incubation systems.The results showed that verapamil could significantly increase the peak plasma concentration (from 60.17ng/mL to 93.66ng/mL) and AUCThese results indicated that verapamil could significantly change the pharmacokinetic profiles of curculigoside in rats, the poor absorption due to P-gp mediated efflux in intestine and high intrinsic clearance rate in rat liver may be the main reason for the poor oral absolute bioavailability of curculigoside.


PubMed | The Chen Group, Qingdao Hiser Medical Group and The Third Peoples Hospital of Qingdao
Type: Journal Article | Journal: Pakistan journal of medical sciences | Year: 2016

To detect the risk factors for deep venous thrombosis (DVT) in patients after neurosurgery.Three hundred and seventy-six patients treated in the department of neurosurgery of our hospital from February 2013 to November 2015 were reviewed retrospectively. The clinical data including age, gender, hospital stay, operation time, occupation type, hypertension, coronary heart disease, diabetes, smoking status, drinking status, postoperative exercises, malignant tumor, and postoperative hormone or dehydrating agent were collected.In this study, 52 patients were included in the DVT group and 295 patients in the Non-DVT group. There was significant difference in age, hypertension, occupation type, malignant tumors, operation time, smoking status, and postoperative exercises between the two groups (p<0.05). However, there was no significant difference in gender, drinking status, coronary heart disease, diabetes, hospital stay, and postoperative hormone or dehydrating agent (p>0.05). In multivariate analysis, age, malignant tumor, hypertension were independent risk factors, while physical labour and postoperative exercises were protective factor for DVT.The postoperative patients with older age, malignant tumor or hypertension should be paid high attention to prevent DVT, and postoperative exercises should be selected as precautionary measures.

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