Time filter

Source Type

Ding Y.,Qingdao University | Yu J.,Qingdao University | Qu P.,Binzhou Medical University | Ma P.,Qingdao Haici Medical Group | Yu Z.,Qingdao University
Inhalation Toxicology | Year: 2015

This study aims to investigate the negative effects of chronic exposure to isoflurane on spermatogenesis and explore the underlying mechanisms. Sixty male rats were randomly allocated to two groups: control group, receiving no treatment, and anesthesia group, administrated exposure to isoflurane (2 ppm) for 25 consecutive days (1 h/day). The negative effects of chronic exposure to isoflurane were evaluated by analyzing the median eminence GnRH content, the relevant hormone levels, some sperm parameters and the mRNA expressions for some reproduction-related genes. Isoflurane significantly decreased the GnRH content and the serum gonadotrophin levels compared with the control group (p < 0.01). Meanwhile, the mRNA expressions of GnRH in hypothalamus, GnRH receptor, luteinizing hormone (LH)-β and follicle-stimulating hormone (FSH)-β in pituitary, and LH receptor and FSH receptor in testes were also significantly inhibited (p < 0.01). Furthermore, the mRNA expressions of androgen receptor (AR), kisspeptin encoded gene (Kiss-1) and its receptor (GPR54) in hypothalamus were significantly diminished by isoflurane (p < 0.01). The results indicated that chronic exposure to isoflurane diminished the synthesis and secretion of GnRH by inhibiting the androgen-AR-Kisspeptin-GPR54 pathway and breaking the hypothalamic-pituitary-gonadal equilibrium, and therefore it could inhibit spermatogenesis. © 2015 © 2015 Taylor & Francis.


PubMed | Qingdao Haici Medical Group, Binzhou Medical University and Qingdao University
Type: Journal Article | Journal: Inhalation toxicology | Year: 2015

This study aims to investigate the negative effects of chronic exposure to isoflurane on spermatogenesis and explore the underlying mechanisms. Sixty male rats were randomly allocated to two groups: control group, receiving no treatment, and anesthesia group, administrated exposure to isoflurane (2ppm) for 25 consecutive days (1h/day). The negative effects of chronic exposure to isoflurane were evaluated by analyzing the median eminence GnRH content, the relevant hormone levels, some sperm parameters and the mRNA expressions for some reproduction-related genes. Isoflurane significantly decreased the GnRH content and the serum gonadotrophin levels compared with the control group (p<0.01). Meanwhile, the mRNA expressions of GnRH in hypothalamus, GnRH receptor, luteinizing hormone (LH)- and follicle-stimulating hormone (FSH)- in pituitary, and LH receptor and FSH receptor in testes were also significantly inhibited (p<0.01). Furthermore, the mRNA expressions of androgen receptor (AR), kisspeptin encoded gene (Kiss-1) and its receptor (GPR54) in hypothalamus were significantly diminished by isoflurane (p<0.01). The results indicated that chronic exposure to isoflurane diminished the synthesis and secretion of GnRH by inhibiting the androgen-AR-Kisspeptin-GPR54 pathway and breaking the hypothalamic-pituitary-gonadal equilibrium, and therefore it could inhibit spermatogenesis.


Gao X.,Chinese Academy of Sciences | Qiao Y.,Qingdao Haici Medical Group | Jia B.,Chinese Academy of Sciences | Jing X.,Chinese Academy of Sciences | And 8 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2012

Previous studies have demonstrated the efficacy of electroacupuncture at ST36 for patients with gastrointestinal motility disorders. While several lines of evidence suggest that the effect may involve vagal reflex, the precise molecular mechanism underlying this process still remains unclear. Here we report that the intragastric pressure increase induced by low frequency electric stimulation at ST36 was blocked by AP-5, an antagonist of N-methyl-D-aspartate receptors (NMDARs). Indeed, stimulating ST36 enhanced NMDAR-mediated, but not 2-amino-3-(5-methyl-3-oxo-1,2-oxazol-4-yl)propanoic-acid-(AMPA-) receptor-(AMPAR-) mediated synaptic transmission in gastric-projecting neurons of the dorsal motor nucleus of the vagus (DMV). We also identified that suppression of presynaptic μ-opioid receptors may contribute to upregulation of NMDAR-mediated synaptic transmission induced by electroacupuncture at ST36. Furthermore, we determined that the glutamate-receptor-2a-(NR2A-) containing NMDARs are essential for NMDAR-mediated enhancement of gastric motility caused by stimulating ST36. Taken together, our results reveal an important role of NMDA receptors in mediating enhancement of gastric motility induced by stimulating ST36. © 2012 Xinyan Gao et al.


Han B.,Qingdao HaiCi Medical Group | Wang H.-X.,Qingdao HaiCi Medical Group | Shao W.,Qingdao Municipal Hospital | Han Z.-L.,Qingdao Municipal Hospital
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2016

OBJECTIVE: To investigate the expressions of miR-34a in bone marrow of adult acute lymphoblastic leukemia (ALL) and its relationship with drug resistance.METHODS: Forty-seven cases of newly diagnosed adult ALL were selected and their bone marrow samples were taken at the time of newly diagnosed and relapsed or complete remission; 26 pairs of specimens were in newly diagnosed-complete remission group, and 21 pairs of specimens were in newly diagnosed-relapse group. The expressions of miR-34a in bone marrow samples, CCRF-CEM cells and resistant CEM-C1 cell strains were detected by real-time quantitative PCR. The expression of miR-34a in CCRF-CEM cells was inhibited and was increased in CEM-C1 cells detected by electroporation transfection method. All the cells were incubated at different concentration of camptothecin. The cell survival was analyzed by CCK-8 method, the cell proliferation inhibition rate (%) and resistance index (RI) were calculated.RESULTS: In newly diagnosed-complete remission group, the miR-34a expression at newly diagnosis was significantly lower than that in complete remission and the control group, the differences were statistically significant (P < 0.05). In newly diagnosed-relapsed group, the miR-34a expressions at newly diagnosis and relapse were lower than those in the control group, the differences were statistically significant (P < 0.05). The expression level in CEM-C1 cells was (2.64 ± 1.37) which significantly lower than that in CCRF-CEM cells (5.14 ± 2.06), the differences were statistically significant (P < 0.05). The expression level of miR-34a in CCRF-CEM cells transfected with miR-34a inhibitor was (3.14 ± 1.15), which significantly lower than that in the miRNA inhibitor-negative control group, the difference was statistically significant (P < 0.05). The cell proliferation inhibition rate of CCRF-CEM cells transfected with miR-34a inhibitor was significantly higher than that in the negative control transfectedcells (P < 0.05), the IC(50) was 28.73 ng/mL and 2167.00 ng/mL respectively, and RI = 75.43. The expression level of miR-34a in CEM-C1 cells transfected with miR-34a mimic was (5.06 ± 1.73), which was significantly higher than that in the miRNA mimics transteted-negative control CEM-C1 cells (P < 0.05). The proliferation inhibition rate in CEM-C1 cells transfected with miR-34a mimic was significantly lower than that in the negative control-transfected cells (P < 0.05). The IC(50) was 112.57 ng/mL and 1.27 ng/mL respectively, and the RI = 88.64.CONCLUSION: The expression of miR-34a in bone marrow samples of adult ALL is low which may be associated with the relapse and drug resistance of ALL.


PubMed | Qingdao HaiCi Medical Group and Qingdao Municipal Hospital
Type: Journal Article | Journal: Zhongguo shi yan xue ye xue za zhi | Year: 2016

To investigate the expressions of miR-34a in bone marrow of adult acute lymphoblastic leukemia (ALL) and its relationship with drug resistance.Forty-seven cases of newly diagnosed adult ALL were selected and their bone marrow samples were taken at the time of newly diagnosed and relapsed or complete remission; 26 pairs of specimens were in newly diagnosed-complete remission group, and 21 pairs of specimens were in newly diagnosed-relapse group. The expressions of miR-34a in bone marrow samples, CCRF-CEM cells and resistant CEM-C1 cell strains were detected by real-time quantitative PCR. The expression of miR-34a in CCRF-CEM cells was inhibited and was increased in CEM-C1 cells detected by electroporation transfection method. All the cells were incubated at different concentration of camptothecin. The cell survival was analyzed by CCK-8 method, the cell proliferation inhibition rate (%) and resistance index (RI) were calculated.In newly diagnosed-complete remission group, the miR-34a expression at newly diagnosis was significantly lower than that in complete remission and the control group, the differences were statistically significant (P < 0.05). In newly diagnosed-relapsed group, the miR-34a expressions at newly diagnosis and relapse were lower than those in the control group, the differences were statistically significant (P < 0.05). The expression level in CEM-C1 cells was (2.64 1.37) which significantly lower than that in CCRF-CEM cells (5.14 2.06), the differences were statistically significant (P < 0.05). The expression level of miR-34a in CCRF-CEM cells transfected with miR-34a inhibitor was (3.14 1.15), which significantly lower than that in the miRNA inhibitor-negative control group, the difference was statistically significant (P < 0.05). The cell proliferation inhibition rate of CCRF-CEM cells transfected with miR-34a inhibitor was significantly higher than that in the negative control transfectedcells (P < 0.05), the IC(50) was 28.73 ng/mL and 2167.00 ng/mL respectively, and RI = 75.43. The expression level of miR-34a in CEM-C1 cells transfected with miR-34a mimic was (5.06 1.73), which was significantly higher than that in the miRNA mimics transteted-negative control CEM-C1 cells (P < 0.05). The proliferation inhibition rate in CEM-C1 cells transfected with miR-34a mimic was significantly lower than that in the negative control-transfected cells (P < 0.05). The IC(50) was 112.57 ng/mL and 1.27 ng/mL respectively, and the RI = 88.64.The expression of miR-34a in bone marrow samples of adult ALL is low which may be associated with the relapse and drug resistance of ALL.


Cai L.-L.,Chinese Academy of Sciences | Li H.,Chinese Academy of Sciences | Liu J.-G.,Chinese Academy of Sciences | Liu L.-T.,Chinese Academy of Sciences | And 4 more authors.
Journal of Chinese Integrative Medicine | Year: 2011

Objective: To observe the effects of early intervention with effective components from a Chinese herbal formula (Huannao Yicong Formula, HNYCF) on behavior and related indicators of cholinergic system in β-amyloid precursor protein (APP) transgenic mice. Methods: Sixty 3-month-old APP695 V7171 transgenic mice were randomly divided into model group, high-dose HNYCF group (2.80 g/(kg·d)), low-dose HNYCF group (1.40 g/(kg·d)) and donepezil group (0.65 mg/(kg·d), with 15 mice in each group. Fifteen non-transgenic mice of the same genetic background were used as normal group The model group and normal group were fed with equal volume of distilled water by gavage. After 6-month continuous medication, the Morris water maze and the passive avoidance test were used to detect the visual spatial learning and memory ability of each mouse. Then the mice were decapitated and their cerebral cortex and hippocampus were isolated to homogenate by sonication. Contents of acetylcholine (ACh) and acetylcholinesterase (AChE) and choline acetyltransferase (ChAT) activity in the homogenate were determined by enzyme-linked immunosorbent assay, and protein contents of cerebral cortex and hippocampus were measured by Coomassie brilliant blue method. Results: Compared with the model group, high- and low-dose HNYCF and donepezil hydrochloride all improved spatial learning of APP mice in the Morris water maze. The ratio of swimming distance in the central area in the high-dose HNYCF group was longer than that in the model group (P<0.05). In the passive avoidance test, high- and low-dose HNYCF and donepezil hydrochloride improved memory function of APP mice by improving the escape latency and reducing the number of errors (P<0.05, P<0.01). High- and low-dose HNYCF and donepezil hydrochloride reduced the content of AChE, increased the activity of ChAT (P<0.01, P<0.05) and improved the content of ACh in hippocampus (P<0.05); high- and low-dose HNYCF and donepezil hydrochloride increased the content of ACh in cortex (P<0.05). Donepezil hydrochloride reduced the content of AChE in cortex (P<0.05), however, high- and low-dose HNYCF had no obvious influence (P>0.05). High- and low-dose HNYCF increased the content of ChAT in cortex (P<0.05), whereas donepezil hydrochloride had no obvious influence (P>0.05). Conclusion: Early intervention with HNYCF effective components can improve the learning and memory ability of APP transgenic mice. The mechanism may be related to enhancing the function of cholinergic system.


Si Q.,No. 27 | Yuan X.,Qingdao Haici Medical Group
ICIC Express Letters | Year: 2016

Decision-makers in sanatoriums often attach great importance to the selection of medical device providers. However, there are many qualitative and quantitative influencing factors in the selection of medical device providers, and various uncertainties exist. Thus, fuzzy based methods are more proper for selecting medical device providers. In this work, we use a straight-line membership function to develop a kind of unascertained measure method, and then propose an evaluation approach of medical device providers, which can identify the evaluation level of each candidate supplier. A case study validates the effectiveness of the developed approach, in comparison with classic fuzzy comprehensive evaluation method. © 2016, ICIC Express Letters ICIC International.


Wei X.-Q.,Shandong University | Wei X.-Q.,Qingdao University | Ma H.-Q.,Qingdao University | Liu A.-H.,Qingdao Haici Medical Group | Zhang Y.Z.,Shandong University
Asian Pacific Journal of Cancer Prevention | Year: 2013

Objective: Photodynamic therapy (PDT) is a promising modality for the treatment of various tumors. In order to assist in optimizing treatment, we applied 5-ALA/PDT in combination with low-dose cisplatin to evaluate cytotoxicity in Hela cells. Methods: Antiproliferative effects of 5-ALA/PDT and cisplatin, alone and in combination, were assessed using MTT assay. To examine levels of apoptosis, Hela cells treated with 5-ALA/PDT, and combination treatment were assessed with Annexin-V/PI by flow cytometry. To investigate the molecular mechanisms underlying alterations in cell proliferation and apoptosis, Western blot analysis was conducted to determine the expression of p53, p21, Bax and Bcl-2 proteins. Results: MTT assays indicated that combination treatment obviously decreased the viability of Hela cells compared to individual drug treatment. In addition, it was confirmed that exposure of Hela cells to 5-ALA/PDT in combination with low-dose cisplatin resulted in more apoptosis in vitro. Synergistic anticancer activity was related to upregulation p53 expression and alteration in expression of p21, Bcl-2 and Bax. Conclusion: Our findings suggest that administration of 5-ALA/PDT in combination with the low-dose cisplatin may be an effective and feasible therapy for cervical cancer.


Gao Z.-D.,Qingdao Haici Medical Group | Pan Q.,Qingdao Haici Medical Group
Chinese Journal of Cancer Prevention and Treatment | Year: 2011

OBJECTIVE: To summarize abroad and non-cervical HPV infection in squamous cell carcinoma of the progress of relevant research, to explore the possible carcinogenic effects of HPV, nonsquamous cell carcinoma of the cervix to provide a theoretical basis for the prevention and treatment. METHODS: PubMed and full-text database retrieval system CNKI journals were searched with "HPV, squamous cell carcinoma," as key words, from January 2005 to June 2011. Total 1134 English literature were retrieved. Inclusion criteria: 1. HPV general characteristics; 2. HPV tumorigenicity; 3. HPV and squamous cell carcinoma, the development of the relationship. According to the inclusion criteria, forty-nine literatured were analyzed. RESULTS: HPV infection are closely related with head, neck, skin, lung squamous cell carcinoma are closely related. HPV infection has a certain correlation with the formation of upper digestive tract squamous cell carcinoma, but further study should be done to the relationship between it and the lower digestive tract squamous cell carcinoma. HPV infection in the lead on various parts of the squamous cell carcinoma may have different genetic mechanisms,and it has been confirmed that in all parts of the HPV-positive squamous cell carcinoma associated with HPV infection has caught a better therapeutic effect. CONCLUSIONS: HPV infection and the formation of non-cervical cancer is closely related parts. However, all parts of HPV infection and associated squamous cell carcinoma subtype is not consistent in different areas still need further large sample test results.


Bi J.-Q.,Qingdao Haici Medical Group | Li Y.-Z.,Qingdao Haici Medical Group | Dai N.-N.,Qingdao Haici Medical Group
Journal of Acupuncture and Tuina Science | Year: 2013

Objective: To observe the clinical efficacy of Three-character-scripture School pediatric tuina for infants with night crying. Methods: Sixty cases with night crying were treated with Three-character- scripture School pediatric tuina, followed by observing the efficacy. Results: Forty-nine cases were recovered, 7 cases improved, and 4 cases failed. The total effective rate was 93. 3%. Conclusion: Three-character-scripture School pediatric tuina is effective for night crying of infants. It should be spread and applied due to its manipulation with less acupoints, simple operation and no pain for infants. © 2013 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.

Loading Qingdao HaiCi Medical Group collaborators
Loading Qingdao HaiCi Medical Group collaborators