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Zhang X.,Shandong University of Traditional Chinese Medicine | Cheng B.,Shandong University of Traditional Chinese Medicine | Cheng B.,Chinese Academy of Sciences | Jing X.,Chinese Academy of Sciences | And 7 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2012

A large number of studies have been conducted to explore the efficacy of electroacupuncture (EA) for the treatment of gastrointestinal motility. While several lines of evidence addressed the basic mechanism of EA on gastrointestinal motility regarding effects of limb and abdomen points, the mechanism for effects of the back points on gastric motility still remains unclear. Here we report that the NMDA receptor (NMDAR) antagonist kynurenic acid inhibited the gastric emptying increase induced by high-intensity EA at BL21 and agonist NMDA enhanced the effect of the same treatment. EA at BL21 enhanced NMDAR, but not AMPA receptor (AMPAR) component of miniature excitatory postsynaptic current (mEPSC) in gastric-projecting neurons of the dorsal motor nucleus of the vagus (DMV). In sum, our data demonstrate an important role of NMDAR-mediated synaptic transmission of gastric-projecting DMV neurons in mediating EA at BL21-induced enhancement of gastric emptying. Copyright © 2012 Xin Zhang et al. Source


Gao X.,Chinese Academy of Sciences | Qiao Y.,Qingdao HaiCi Medical Group | Jia B.,Chinese Academy of Sciences | Jing X.,Chinese Academy of Sciences | And 8 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2012

Previous studies have demonstrated the efficacy of electroacupuncture at ST36 for patients with gastrointestinal motility disorders. While several lines of evidence suggest that the effect may involve vagal reflex, the precise molecular mechanism underlying this process still remains unclear. Here we report that the intragastric pressure increase induced by low frequency electric stimulation at ST36 was blocked by AP-5, an antagonist of N-methyl-D-aspartate receptors (NMDARs). Indeed, stimulating ST36 enhanced NMDAR-mediated, but not 2-amino-3-(5-methyl-3-oxo-1,2-oxazol-4-yl)propanoic-acid-(AMPA-) receptor-(AMPAR-) mediated synaptic transmission in gastric-projecting neurons of the dorsal motor nucleus of the vagus (DMV). We also identified that suppression of presynaptic μ-opioid receptors may contribute to upregulation of NMDAR-mediated synaptic transmission induced by electroacupuncture at ST36. Furthermore, we determined that the glutamate-receptor-2a-(NR2A-) containing NMDARs are essential for NMDAR-mediated enhancement of gastric motility caused by stimulating ST36. Taken together, our results reveal an important role of NMDA receptors in mediating enhancement of gastric motility induced by stimulating ST36. © 2012 Xinyan Gao et al. Source


Qi D.,Shandong University of Traditional Chinese Medicine | Qiao Y.,Qingdao HaiCi Medical Group | Zhang X.,Shandong University of Traditional Chinese Medicine | Yu H.,Shandong University of Traditional Chinese Medicine | And 4 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2012

Previous studies demonstrated that Alzheimers disease was considered as the consequence produced by deficiency of Kidney essence. However, the mechanism underlying the symptoms also remains elusive. Here we report that spatial learning and memory, escape, and swimming capacities were damaged significantly in Kidney-yang deficiency rats. Indeed, both hippocampal A 40 and 42 increases in Kidney-yang deficiency contribute to the learning and memory impairments. Specifically, damage of synaptic plasticity is involved in the learning and memory impairment of Kidney-yang deficiency rats. We determined that the learning and memory damage in Kidney-yang deficiency due to synaptic plasticity impairment and increases of A 40 and 42 was not caused via NMDA receptor internalization induced by A increase. -Adrenergic receptor agonist can rescue the impaired long-term potential (LTP) in Kidney-yang rats. Taken together, our results suggest that spatial learning and memory inhibited in Kidney-yang deficiency might be induced by A increase and the decrease of 2 receptor function in glia. Copyright © 2012 Dongmei Qi et al. Source


Ding Y.,Qingdao University | Yu J.,Qingdao University | Qu P.,Binzhou Medical University | Ma P.,Qingdao HaiCi Medical Group | Yu Z.,Qingdao University
Inhalation Toxicology | Year: 2015

This study aims to investigate the negative effects of chronic exposure to isoflurane on spermatogenesis and explore the underlying mechanisms. Sixty male rats were randomly allocated to two groups: control group, receiving no treatment, and anesthesia group, administrated exposure to isoflurane (2 ppm) for 25 consecutive days (1 h/day). The negative effects of chronic exposure to isoflurane were evaluated by analyzing the median eminence GnRH content, the relevant hormone levels, some sperm parameters and the mRNA expressions for some reproduction-related genes. Isoflurane significantly decreased the GnRH content and the serum gonadotrophin levels compared with the control group (p < 0.01). Meanwhile, the mRNA expressions of GnRH in hypothalamus, GnRH receptor, luteinizing hormone (LH)-β and follicle-stimulating hormone (FSH)-β in pituitary, and LH receptor and FSH receptor in testes were also significantly inhibited (p < 0.01). Furthermore, the mRNA expressions of androgen receptor (AR), kisspeptin encoded gene (Kiss-1) and its receptor (GPR54) in hypothalamus were significantly diminished by isoflurane (p < 0.01). The results indicated that chronic exposure to isoflurane diminished the synthesis and secretion of GnRH by inhibiting the androgen-AR-Kisspeptin-GPR54 pathway and breaking the hypothalamic-pituitary-gonadal equilibrium, and therefore it could inhibit spermatogenesis. © 2015 © 2015 Taylor & Francis. Source


Si Q.,No. 27 | Yuan X.,Qingdao HaiCi Medical Group
ICIC Express Letters | Year: 2016

Decision-makers in sanatoriums often attach great importance to the selection of medical device providers. However, there are many qualitative and quantitative influencing factors in the selection of medical device providers, and various uncertainties exist. Thus, fuzzy based methods are more proper for selecting medical device providers. In this work, we use a straight-line membership function to develop a kind of unascertained measure method, and then propose an evaluation approach of medical device providers, which can identify the evaluation level of each candidate supplier. A case study validates the effectiveness of the developed approach, in comparison with classic fuzzy comprehensive evaluation method. © 2016, ICIC Express Letters ICIC International. Source

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