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Li J.-Y.,Qingdao Fuwai Cardiovascular Hospital | Yang C.-W.,Qingdao Fuwai Cardiovascular Hospital | Feng Q.,Qingdao Fuwai Cardiovascular Hospital | Wu W.-H.,Peking Union Medical College | And 6 more authors.
Chinese Journal of Medical Imaging Technology | Year: 2010

Objective: To explore the value of multi-slice spiral computed tomography angiography (MSCTA) and color Doppler flow imaging (CDFI) in evaluation of carotid artery atherosclerostic plaques in patients with cerebral infarction. Methods: Totally 195 consecutive patients with cerebral infarction were enrolled. All patients underwent examinations of carotid artery with both MSCTA and CDFI within 7 days. Intima-media thickness (IMT)/carotid artery wall thickness (CAWT), and the surface morphology, density, volume of the atherosclerotic plaques were analyzed. The results were divided into 4 types including normal, IMT/CAWT thickening, stable plaque and vulnerable plaque. Results: A total of 390 carotid arteries were analyzed. MSCTA showed 71 (18.21%) normal, 87 (22.31%) CAWT thickeningm 232 (59.49%) plaques including 140 (35.90%) stable plaques and 92 (23.59%) vulnerable plaques (ulcerated of 5). CDFI showed 80 (20.51%) normal, 86 (22.05%) IMT thickening, 224 (57.44%) plaques including 136 (34.87%) stable plaques and 88 (22.56%) vulnerable plaques (ulcerated of 4). The overall concordance of morphology characteristic of carotid artery between both MSCTA and CDFI was 72.60% (Kappa=0.63, P<0.05). Conclusion: MSCTA has good agreement with CDFI in assessing carotid atherosclerosis in patients with cerebral infarction. CDFI can be used as the first choice of the carotid artery imaging, while MSCTA should be routine examination in patients with vascular lesion found with CDFI.


Xia W.,Qingdao Municipal Hospital | Wang Y.,Qingdao Municipal Hospital | Duan T.,Qingdao Fuwai Cardiovascular Hospital | Rong Y.,Qingdao Municipal Hospital | And 2 more authors.
Thrombosis Research | Year: 2015

Introduction Atrial fibrillation (AF) is associated with increased thrombo-embolic events, with thrombi most frequently located in the left atrial appendage (LAA). Asymmetric dimethylarginine (ADMA), a potent endogenous inhibitor of nitric oxide synthase, is elevated in subjects with AF. We investigated the relationship between ADMA and risk of LAA thrombus in patients with non-valvular AF. Methods and results A total of 223 consecutive patients with non-valvular AF were enrolled (63 ± 10 years, 65% male). LAA thrombus was detected in 27 subjects by transesophageal echocardiography. Serum ADMA was significantly increased (2.5 ± 0.6 μmol/L vs 1.8 ± 0.4 μmol/L, p < 0.001), while serum nitrite/nitrate was significantly reduced in patients with LAA thrombus (30.3 ± 7.1 μmol/L vs 48.4 ± 9.3 μmol/L, p < 0.001). ADMA was positively correlated with age and left atrium diameter, and was negatively correlated with nitrite/nitrate and LAA peak flow velocity. The area under the receiver operating characteristic curve of ADMA predicting thrombus was 0.84. In multivariate logistic regression analysis, ADMA (OR 4.0, 95% CI 1.2-13.0; p = 0.003) was one of independent risk factors for LAA thrombus. Conclusions Our study suggested that high ADMA was independently associated with the presence of LAA thrombus in patients with non-valvular AF. © 2015 Elsevier Ltd. All rights reserved.


PubMed | Qingdao Fuwai Cardiovascular Hospital and Qingdao Municipal Hospital
Type: Journal Article | Journal: Thrombosis research | Year: 2015

Atrial fibrillation (AF) is associated with increased thrombo-embolic events, with thrombi most frequently located in the left atrial appendage (LAA). Asymmetric dimethylarginine (ADMA), a potent endogenous inhibitor of nitric oxide synthase, is elevated in subjects with AF. We investigated the relationship between ADMA and risk of LAA thrombus in patients with non-valvular AF.A total of 223 consecutive patients with non-valvular AF were enrolled (63 10 years, 65% male). LAA thrombus was detected in 27 subjects by transesophageal echocardiography. Serum ADMA was significantly increased (2.5 0.6 mol/L vs 1.8 0.4 mol/L, p<0.001), while serum nitrite/nitrate was significantly reduced in patients with LAA thrombus (30.3 7.1 mol/L vs 48.4 9.3 mol/L, p<0.001). ADMA was positively correlated with age and left atrium diameter, and was negatively correlated with nitrite/nitrate and LAA peak flow velocity. The area under the receiver operating characteristic curve of ADMA predicting thrombus was 0.84. In multivariate logistic regression analysis, ADMA (OR 4.0, 95% CI 1.2-13.0; p=0.003) was one of independent risk factors for LAA thrombus.Our study suggested that high ADMA was independently associated with the presence of LAA thrombus in patients with non-valvular AF.


Wang X.,Qingdao University | Jiang F.,Qingdao University | Liang Y.,Qingdao University | Xu L.,Heze Medical College | And 4 more authors.
PLoS ONE | Year: 2014

Objective: The purpose of our study is to investigate the relationship between IL-1β -31C/T (rs1143627) and -511T/C (rs16944) polymorphisms and the preeclampsia (PE), and analyze the Linkage disequilibrium (LD) and haplotype frequency of the two polymorphism loci.Methods: Polymorphisms at -31C/T and -511T/C of IL-1β were genotyped with the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 232 PE and 447 control subjects. Genotype and allele frequencies between case-control groups were compared by chi-square(χ2) tests. Two-point LD and haplotype frequency analyses were done with the software Haploview4.2.Results: Significant statistical differences were found between PE and control groups regarding genotype and allele frequencies of the two polymorphisms of IL-1β (For IL-1β -31C/T: χ2 = 11.478, P = 0.003; For IL-1β-511T/C: χ2 = 9.687, P = 0.008). LD analysis revealed that the IL-1β -31C/T SNP was in high LD with the IL-1β-511C/T SNP(D′ = 0.92, r2 = 0.79). Both CT and TC haplotypes showed significant differences between case and control groups. Only the plasma level of Prothrombin Time had a significantly statistical difference among TT, CT and CC groups of the preeclamptic two polymorphisms of IL-1β-31C/T and -511T/C (for IL-1β-31C/T, F = 1.644, P = 0.01; F = 1.587, P = 0.016).Conclusion: Our results revealed IL-1β was associated with the PE in Chinese Han population. The CT haplotype may increase the risk of PE, while haplotype TC could be considered as a protective haplotype of PE. © 2014 PLOS ONE.


Zhao W.,Qingdao University | Zhang B.,Qingdao University | Guo X.,Qingdao Fuwai Cardiovascular Hospital | Zhang X.,Qingdao University | And 3 more authors.
Cancer Biomarkers | Year: 2014

BACKGROUND: Hilar cholangiocarcinoma (HCC) is defined as a cholangiocarcinoma located in the bifurcation of the right and left bile duct, constituting 40%-60% of all reported cholangiocarcinoma and 58%-75% of the extrahepatic cholangiocarcinoma. In this study, we aim to investigate the expression of Ki-67, Bax and p73 in the patients with HCC, and identify their potential roles in the prognosis of HCC. OBJECTIVE: Thirty five HCC patients (male: 25, female: 10) with an average age of 62.8 years were diagnosed with HCC according to the pathological tests. A total of 20 cadavers with normal hilar bile ducts were used as control. METHODS: The expression of Ki-67, Bax, and p73 was determined using immunohistochemical analysis. RESULTS: The pathological test indicated that Ki-67 expression increased with the stage of the disease, and the infiltration of the cancer cells. Statistical difference was noted in the expression of Bax LI between the patients with HCC and normal control (32.4 ± 17.6 vs 11.2 ± 7.9, P< 0.01). Statistical difference was observed between the patients with or without lymphatic metastasis, and those of various differentiation stages (P < 0.05). Among the 35 patients with HCC, expression of p73 was observed in 11 patients (31.43%). Compared with normal control, remarkable increase of p73 expression was noted in the HCC patients (P < 0.01). Additionally, no statistical difference was noted in the expression of p73 in the patients with various disease stages, and those with or without metastasis (P> 0.05). CONCLUSIONS: Ki-67, Bax, and p73 could be used as biomarkers for the prognosis of HCC. © 2014 - IOS Press and the authors. All rights reserved.


PubMed | Qingdao Fuwai Cardiovascular Hospital and Qingdao University
Type: Journal Article | Journal: Cancer biomarkers : section A of Disease markers | Year: 2014

Hilar cholangiocarcinoma (HCC) is defined as a cholangiocarcinoma located in the bifurcation of the right and left bile duct, constituting 40%-60% of all reported cholangiocarcinoma and 58%-75% of the extrahepatic cholangiocarcinoma. In this study, we aim to investigate the expression of Ki-67, Bax and p73 in the patients with HCC, and identify their potential roles in the prognosis of HCC.Thirty five HCC patients (male: 25, female: 10) with an average age of 62.8 years were diagnosed with HCC according to the pathological tests. A total of 20 cadavers with normal hilar bile ducts were used as control.The expression of Ki-67, Bax, and p73 was determined using immunohistochemical analysis.The pathological test indicated that Ki-67 expression increased with the stage of the disease, and the infiltration of the cancer cells. Statistical difference was noted in the expression of Bax LI between the patients with HCC and normal control (32.4 17.6 vs 11.2 7.9, P< 0.01). Statistical difference was observed between the patients with or without lymphatic metastasis, and those of various differentiation stages (P < 0.05). Among the 35 patients with HCC, expression of p73 was observed in 11 patients (31.43%). Compared with normal control, remarkable increase of p73 expression was noted in the HCC patients (P < 0.01). Additionally, no statistical difference was noted in the expression of p73 in the patients with various disease stages, and those with or without metastasis (P> 0.05).Ki-67, Bax, and p73 could be used as biomarkers for the prognosis of HCC.

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