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Chen N.,Shandong Peanut Research Institute | Yang Q.,Shandong Peanut Research Institute | Su M.,Qingdao Entry Exit Inspection and Quarantine Bureau | Pan L.,Shandong Peanut Research Institute | And 7 more authors.
Plant Molecular Biology Reporter | Year: 2012

Several ethylene-responsive element binding factor (ERF) family proteins have been demonstrated to play important roles in transcriptional regulation of a variety of biological processes, including responses to environmental conditions such as drought, salt, and cold. To date, there have been no reports on this family in peanut. In this study, 40 ESTs in a peanut cDNA library, whose amino acid sequences contained AP2/ ERF domain, have been identified. Of these, full-length sequences of six genes were cloned. Sequence analysis indicated that all six proteins contained only one AP2/ ERF domain and should belong to the ERF family. The six genes were designated Arachis hypogaea ERF 1-6 (AhERF1-6). The expression patterns of AhERF1-6 were analyzed under cold, salt and drought stress. The results indicated that the expression of AhERF4 and AhERF6 were rapidly and substantially enhanced under abiotic stress. The expression of AhERF1 and AhEERF5 were slightly enhanced under certain stress conditions. Some genes were down-regulated when under stress, such as AhEERF3 in leaves under salt stress and AhERF2 in leaves under drought stress. Interestingly, the expression of AhERF3 and AhERF5 exhibited contrary expression patterns in peanut leaves and roots upon PEG treatment. These results suggested that different ERF proteins may have different functions in peanut abiotic stress acclimation. © 2012 Springer-Verlag.

Wang L.,Chinese Academy of Agricultural Sciences | Yao Y.,Chinese Academy of Agricultural Sciences | He Z.,Chinese Academy of Agricultural Sciences | Wang D.,Chinese Academy of Agricultural Sciences | And 2 more authors.
Journal of Cereal Science | Year: 2013

High-performance liquid chromatography (HPLC) was used to determine the distribution of phenolic acids in wheat flours produced from five milling extraction rates ranging from 60% to 100% in four cultivars sown in two locations in the 2008-2009 season. Considerable variation was observed in free and bound phenolic acids, and their components in flours with different extraction rates. Most phenolic acids, including the component ferulic, were present in the bound form (94.0%). Ferulic (51.0%) was the predominant phenolic acid in wheat grain, and caffeic (22.8%) and p-coumaric (17.6%) acids were abundant. The phenolic acids and their components were all significantly influenced by effects of cultivar, milling, location, and cultivar × milling interaction, with milling effect being the predominant. The proportions of phenolic compounds varied considerably among milling extractions and cultivars, and their levels depended on both initial grain concentrations and on selection of milling extraction that was incorporated into the final product. The grain phenolic acid concentrations determined ranged from 54 μg g-1 in flour produced at 60% extraction rate to 695 μg g-1 in flour produced at 100% extraction rate, indicating their higher concentrations in bran associated with cell wall materials. Therefore, wholemeal wheat products maximize health benefits and are strongly recommended for use in food processing. © 2012.

Yu H.T.,Shandong Peanut Research Institute | Yang W.Q.,Shandong Peanut Research Institute | Tang Y.Y.,Shandong Peanut Research Institute | Wang X.Z.,Shandong Peanut Research Institute | And 4 more authors.
Grasas y Aceites | Year: 2013

FAD2A and FAD2B are homoeologous genes from A and B genomes in cultivated peanuts (Arachis hypogaea L.) encoding fatty acid desaturates which convert oleate to linoleate. To study the genetics of oleate and breed high oleate peanut cultivars, a simple allele specific-PCR (AS-PCR) protocol for the accurate genotyping of FAD2A/FAD2B was developed to discriminate the wild and mutant allele of both genes (FAD2A 448 G > A and FAD2B 441-442insA). The results may serve to develop a feasible procedure for producing highly desired high oleate peanut cultivars through hybridization.

Chen N.,Shandong Peanut Research Institute | Yang Q.,Shandong Peanut Research Institute | Pan L.,Shandong Peanut Research Institute | Chi X.,Shandong Peanut Research Institute | And 6 more authors.
Gene | Year: 2014

The MYB superfamily constitutes one of the most abundant groups of transcription factors and plays central roles in developmental processes and defense responses in plants. In the work described in this article, 30 unique peanut MYB genes that contained full-length cDNA sequences were isolated. The 30 genes were grouped into three categories: one R1R2R3-MYB, nine R2R3-MYBs and 20 MYB-related members. The sequence composition of the R2 and R3 repeats was conserved among the nine peanut R2R3-MYB proteins. Phylogenetic comparison of the members of this superfamily between peanut and Arabidopsis revealed that the putative functions of some peanut MYB proteins were clustered into the Arabidopsis functional groups. Expression analysis during abiotic stress identified a group of MYB genes that responded to at least one stress treatment. This is the first comprehensive study of the MYB gene family in peanut. © 2013 Elsevier B.V.

Wan J.-H.,China University of Petroleum - East China | Han Z.-Z.,China University of Petroleum - East China | Han Z.-Z.,Qingdao Agricultural University | Song X.-X.,Qingdao Entry Exit Inspection and Quarantine Bureau | Liu J.,Qingdao Agricultural University
Faguang Xuebao/Chinese Journal of Luminescence | Year: 2016

In order to identity different oil spill by the fluorescence phenomena of oil and its products, the hyperspectral images data of six varieties of oil spills samples were collected under two kind of illuminations (UV and halogen lights) using hyperspectral imaging camera. In the spectral region of 400-720 nm (10 nm spectral bandwidth), four radiation index were obtained which include radiation index of individual spectral bands and the difference, ratio, and the normalized difference radiation index of consecutive spectral bands. Then, a novel method composed of Fisher and PCA to identify most significant wavelengths was proposed, and a classified model based on REF-SVM and the proposed method was established. By comparison, it is found that the different radiation index, light fusions and model consensus of feather selected method all can improve the accuracy of recognition rate. The overall accuracy rate by our method is above 99.1%, which is obviously higher than traditional methods only use one method. The experiment results show that the multi-pattern fusion can effectively improve the recognition rate of marine oil spill. © 2016, Science Press. All right reserved.

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