Qingdao Entry Exit Inspection and Quarantine Bureau

Qingdao, China

Qingdao Entry Exit Inspection and Quarantine Bureau

Qingdao, China

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Chen C.,Jiangxi Agricultural University | Wei X.,Qingdao Entry Exit Inspection and Quarantine Bureau | Xiao H.,Jiangxi Agricultural University | He H.,Jiangxi Agricultural University | And 2 more authors.
PLoS ONE | Year: 2014

The fall webworm, Hyphantria cunea (Drury), enters facultative diapause as a pupa in response to short-day conditions during autumn. Photoperiodic response curves showed that the critical day length for diapause induction was 14 h 30 min, 14 h 25 min and 13 h 30 min at 22, 25 and 28°C, respectively. The photoperiodic responses under non-24 h light-dark cycles demonstrated that night length played an essential role in the determination of diapause. Experiments using a short day length interrupted by a 1-h light pulse exhibited two troughs of diapause inhibition and the effect of diapause inhibition was greater in the early scotophase than in the late scotophase. The diapause-inducing short day lengths of 8, 10 and 12 h evoked greater intensities of diapause than did 13 and 14 h. Diapause can be terminated without exposure to chilling, but chilling at 5°C for 90 and 120 d significantly accelerated diapause development, reduced mortality, and synchronized adult emergence. Additionally, the potential for H. cunea from the temperate region (Qingdao) to emerge and overwinter under field conditions in subtropical regions (Nanchang) of China was evaluated. Pupae that were transferred to Nanchang in early July showed a 60% survival rate and extremely dispersed pupal period (from 12 to 82 days), suggesting that some pupae may undergo summer diapause. Diapausing temperate region pupae that were moved out-of-doors in Nanchang during October showed approximately 20% overwintering survival; moreover, those pupae that overwintered successfully emerged the next spring during a period when their host plants would be available. The results indicate that this moth has the potential to expand its range into subtropical regions of China. © 2014 Chen et al.


PubMed | Qingdao Entry Exit Inspection and Quarantine Bureau, Yantai Jieke Inspection Service Co. and Yantai Entry Exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Se pu = Chinese journal of chromatography | Year: 2014

A method for the simultaneous determination of the residues of 11 quinolones in hotpot ingredients by quick, easy, cheap, effective, rugged and safe (QuEChERS) extraction and ultra performance liquid chromatography-tandem mass spectrometric method (UPLC-MS/MS) was established. The sample was extracted with acetonitrile (containing 5% formic acid) and followed by stratifying with a salting-out agent. Clean-up of the extracts was processed by C18 and PSA, a modified QuEChERS procedure. The analytes were then separated on a Poroshell 120 EC-C18 column, and finally detected by tandem mass spectrometry in positive ESI mode. The linearity of all the 11 quinolones in the range from 1.0 to 100.0 microg/kg had correlation coefficients greater than 0.998. The limits of detection (LOD) of the method were from 1.8 to 3.1 microg/kg and the limits of quantification (LOQ) were from 6.0 to 10.3 microg/kg. The average recoveries of the 11 quinolones were in the range from 70.1% to 100.3%, with relative standard deviations from 2.42% to 10.88%. The established method is sensitive and of good recoveries. It can be applied as a rapid and reliable method for the determination of the 11 quinolones in hotpot ingredients.


Duan W.,Ningbo Entry Exit Inspection and Quarantine Bureau | Duan W.,Ningbo Academy of Inspection and Quarantine | Guo L.,Ningbo Entry Exit Inspection and Quarantine Bureau | Guo L.,Ningbo Academy of Inspection and Quarantine | And 7 more authors.
Journal of Applied Phycology | Year: 2012

During the summer of 2008 and 2009, massive algal blooms repeatedly broke out in the Yellow Sea of China. These were undoubtedly caused by the accumulations of one or more species in the macroalgal genus Ulva. In previous reports, morphological observation indicated that the species involved in this phenomenon is Ulva prolifera but molecular analyses indicated that the species belongs to an Ulva linza-procera-prolifera (LPP) clade. Correct identification of the bloom species is required to understand and manage the blooms, but the taxonomic status of the bloom species remains unclear. In the current study, the taxonomic status of 22 selected specimens from the Yellow Sea was assessed by using both morphological and molecular (ITS and rbcL sequences) data. In addition, 5S rDNA analyses were performed for those samples clustering in the LPP clade, and phylogenetic tree and ribotype analyses were constructed for determining the possible origin of the bloom. Three free-floating and two attached Ulva species were distinguished and described: Ulva compressa Linnaeus and Ulva pertusa Kjellman were found in free-floating samples; U. linza Linnaeus was found on rocks; and U. prolifera O. F. Müller was found in both habitats. Diversity in free-floating Ulva of the Yellow Sea appears to be greater than previously thought. The dominant free-floating Ulva species, U. prolifera, was not closely related to local populations attached to rocks but was closely related to populations from Japan. © 2011 Springer Science+Business Media B.V.


Wang L.,Chinese Academy of Agricultural Sciences | Yao Y.,Chinese Academy of Agricultural Sciences | He Z.,Chinese Academy of Agricultural Sciences | Wang D.,Chinese Academy of Agricultural Sciences | And 2 more authors.
Journal of Cereal Science | Year: 2013

High-performance liquid chromatography (HPLC) was used to determine the distribution of phenolic acids in wheat flours produced from five milling extraction rates ranging from 60% to 100% in four cultivars sown in two locations in the 2008-2009 season. Considerable variation was observed in free and bound phenolic acids, and their components in flours with different extraction rates. Most phenolic acids, including the component ferulic, were present in the bound form (94.0%). Ferulic (51.0%) was the predominant phenolic acid in wheat grain, and caffeic (22.8%) and p-coumaric (17.6%) acids were abundant. The phenolic acids and their components were all significantly influenced by effects of cultivar, milling, location, and cultivar × milling interaction, with milling effect being the predominant. The proportions of phenolic compounds varied considerably among milling extractions and cultivars, and their levels depended on both initial grain concentrations and on selection of milling extraction that was incorporated into the final product. The grain phenolic acid concentrations determined ranged from 54 μg g-1 in flour produced at 60% extraction rate to 695 μg g-1 in flour produced at 100% extraction rate, indicating their higher concentrations in bran associated with cell wall materials. Therefore, wholemeal wheat products maximize health benefits and are strongly recommended for use in food processing. © 2012.


Chen N.,Shandong Peanut Research Institute | Yang Q.,Shandong Peanut Research Institute | Su M.,Qingdao Entry Exit Inspection and Quarantine Bureau | Pan L.,Shandong Peanut Research Institute | And 7 more authors.
Plant Molecular Biology Reporter | Year: 2012

Several ethylene-responsive element binding factor (ERF) family proteins have been demonstrated to play important roles in transcriptional regulation of a variety of biological processes, including responses to environmental conditions such as drought, salt, and cold. To date, there have been no reports on this family in peanut. In this study, 40 ESTs in a peanut cDNA library, whose amino acid sequences contained AP2/ ERF domain, have been identified. Of these, full-length sequences of six genes were cloned. Sequence analysis indicated that all six proteins contained only one AP2/ ERF domain and should belong to the ERF family. The six genes were designated Arachis hypogaea ERF 1-6 (AhERF1-6). The expression patterns of AhERF1-6 were analyzed under cold, salt and drought stress. The results indicated that the expression of AhERF4 and AhERF6 were rapidly and substantially enhanced under abiotic stress. The expression of AhERF1 and AhEERF5 were slightly enhanced under certain stress conditions. Some genes were down-regulated when under stress, such as AhEERF3 in leaves under salt stress and AhERF2 in leaves under drought stress. Interestingly, the expression of AhERF3 and AhERF5 exhibited contrary expression patterns in peanut leaves and roots upon PEG treatment. These results suggested that different ERF proteins may have different functions in peanut abiotic stress acclimation. © 2012 Springer-Verlag.


Chen N.,Shandong Peanut Research Institute | Yang Q.,Shandong Peanut Research Institute | Pan L.,Shandong Peanut Research Institute | Chi X.,Shandong Peanut Research Institute | And 6 more authors.
Gene | Year: 2014

The MYB superfamily constitutes one of the most abundant groups of transcription factors and plays central roles in developmental processes and defense responses in plants. In the work described in this article, 30 unique peanut MYB genes that contained full-length cDNA sequences were isolated. The 30 genes were grouped into three categories: one R1R2R3-MYB, nine R2R3-MYBs and 20 MYB-related members. The sequence composition of the R2 and R3 repeats was conserved among the nine peanut R2R3-MYB proteins. Phylogenetic comparison of the members of this superfamily between peanut and Arabidopsis revealed that the putative functions of some peanut MYB proteins were clustered into the Arabidopsis functional groups. Expression analysis during abiotic stress identified a group of MYB genes that responded to at least one stress treatment. This is the first comprehensive study of the MYB gene family in peanut. © 2013 Elsevier B.V.


Wan J.-H.,China University of Petroleum - East China | Han Z.-Z.,China University of Petroleum - East China | Han Z.-Z.,Qingdao Agricultural University | Song X.-X.,Qingdao Entry exit Inspection and Quarantine Bureau | Liu J.,Qingdao Agricultural University
Faguang Xuebao/Chinese Journal of Luminescence | Year: 2016

In order to identity different oil spill by the fluorescence phenomena of oil and its products, the hyperspectral images data of six varieties of oil spills samples were collected under two kind of illuminations (UV and halogen lights) using hyperspectral imaging camera. In the spectral region of 400-720 nm (10 nm spectral bandwidth), four radiation index were obtained which include radiation index of individual spectral bands and the difference, ratio, and the normalized difference radiation index of consecutive spectral bands. Then, a novel method composed of Fisher and PCA to identify most significant wavelengths was proposed, and a classified model based on REF-SVM and the proposed method was established. By comparison, it is found that the different radiation index, light fusions and model consensus of feather selected method all can improve the accuracy of recognition rate. The overall accuracy rate by our method is above 99.1%, which is obviously higher than traditional methods only use one method. The experiment results show that the multi-pattern fusion can effectively improve the recognition rate of marine oil spill. © 2016, Science Press. All right reserved.


Wang C.T.,Shandong Peanut Research Institute | Wang X.Z.,Shandong Peanut Research Institute | Tang Y.Y.,Shandong Peanut Research Institute | Wu Q.,Shandong Peanut Research Institute | And 3 more authors.
Advanced Materials Research | Year: 2014

Eighty-four, 61 and 105 groundnut seeds, including high oleate genotypes and F2 seeds of normal oleate × high oleate crosses, genotypes with high or low oil and F2 seeds from high oil × low oil crosses, and randomly selected samples representing various origins, different seed sizes, and varied seed coat color and protein content, were used to develop the NIRS models for main fatty acids, oil and protein. For oleic, linoleic and palmitic acid, the optimized spectrum pretreatment method was first derivative plus multiplicative scattering correction; for stearic acid and the four bad fatty acids, first derivative plus vector normalization. The Rcal 2 and RMSECV for oleic acid were 97.20% and 2.65%; for linoleic acid, 96.90% and 2.40%; and for palmitic acid, 93.39% and 0.53%, respectively. The best spectrum pretreatment method for oil and protein was first derivative plus multiplicative scattering correction and min-max normalization. For oil and protein, the Rcal 2 was 89.06% and 91.45%, and RMSECV, 0.89% and 0.78%, respectively. The NIRS models can be used to develop groundnut cultivars both with high oil and with high oleate to cater the growing need for biodiesel production. © (2014) Trans Tech Publications, Switzerland.


PubMed | University of Hawaii at Manoa, Chinese Academy of Sciences and Qingdao Entry Exit Inspection and Quarantine Bureau
Type: Journal Article | Journal: Journal of separation science | Year: 2016

The current theory of programmed temperature gas chromatography considers that solutes are focused by the stationary phase at the column head completely and does not explicitly recognize the different effects of initial temperature (To ) and heating rate (rT ) on the retention time or temperature of a homologue series. In the present study, n-alkanes, 1-alkenes, 1-alkyl alcohols, alkyl benzenes, and fatty acid methyl esters standards were used as model chemicals and were separated on two nonpolar columns, one moderately polar column and one polar column. Effects of To and rT on the retention of nonstationary phase focusing solutes can be explicitly described with isothermal and cubic equation models, respectively. When the solutes were in the stationary phase focusing status, the single-retention behavior of solutes was observed. It is simple, dependent upon rT only and can be well described by the cubic equation model that was visualized through four sequential slope analyses. These observed dual- and single-retention behaviors of solutes were validated by various experimental data, physical properties, and computational simulation.


PubMed | Shandong Agricultural University and Qingdao Entry Exit Inspection And Quarantine Bureau
Type: Journal Article | Journal: Poultry science | Year: 2015

The diagnosis of avian leukosis virus subgroup J (ALV-J) infection in Chinese Partridge Shank chickens was confirmed by necropsy, histopathological examinations, antibody tests, viral isolation, immunofluorescence assays, and sequence analysis. Myelocytoma, myeloma, and fibrosarcoma were simultaneously found in Partridge Shank flock with ALV-J infection. Sequence analysis of the env genes of ALV-J demonstrated that both gp85 and gp37 were highly homologous among the three strains from local chickens of those among ALV-J strains isolated from white meat-type chickens. The phylogenetic trees indicated that the three strains isolated in this study were closely related to reference strains isolated in so-called Chinese yellow chickens and some strains isolated from white meat-type chickens, both from the USA and China. The observed ALV-J infection was the first report on Partridge Shank chickens, and myelocytoma, myeloma, and fibrosarcoma were found at the same time in this batch of local chickens.

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