Qiannan Medical College for Nationalities

Duyun, China

Qiannan Medical College for Nationalities

Duyun, China
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Zeng X.,Nanjing University | Zeng X.,Nanjing Medical University | Yuan Y.,Nanjing Medical University | Wang T.,Nanjing Medical University | And 6 more authors.
Journal of Nanobiotechnology | Year: 2017

Background: Currently, graphene oxide has attracted growing attention as a drug delivery system due to its unique characteristics. Furthermore, utilization of microRNAs as biomarkers and therapeutic strategies would be particularly attractive because of their biological mechanisms and relatively low toxicity. Therefore, we have developed functionalized nanocompounds consisted of graphene oxide, quantum dots and microRNA, which induced cancer cells apoptosis along with targeted imaging. Results: In the present study, we synthesized a kind of graphene-P-gp loaded with miR-122-InPZnS quantum dots nanocomposites (GPMQNs) that, in the presence of glutathione, provides controlled release of miR-122. The miR-122 actively targeted liver tumor cells and induced their apoptosis, including drug-resistant liver tumor cells. We also explored the near-infrared fluorescence and potential utility for targeting imaging of InP@ZnS quantum dots. To further understand the molecular mechanism of GPMQNs-induced apoptosis of drug-resistant HepG2/ADM hepatoma cells, the relevant apoptosis proteins and signal pathways were explored in vitro and in vivo. Furthermore, near-infrared GPMQNs, which exhibited reduced photon scattering and auto-fluorescence, were applied for tumor imaging in vivo to allow for deep tissue penetration and three-dimensional imaging. Conclusion: In conclusion, techniques using GPMQNs could provide a novel targeted treatment for liver cancer, which possessed properties of targeted imaging, low toxicity, and controlled release. © 2017 The Author(s).


Hu X.-Y.,Nanjing Southeast University | Hu X.-Y.,Qiannan Medical College for Nationalities | Meng T.-H.,Qiannan Medical College for Nationalities | Zhang R.-G.,Qiannan Medical College for Nationalities | And 2 more authors.
Faguang Xuebao/Chinese Journal of Luminescence | Year: 2017

The fluorescent nanoprobe for the detection of DNA was established by utilizing mercaptopropionic acid coated InP@ZnS QDs and Dured. In this nanoprobe. The InP@ZnS QDs/Dured fluorescent nanoprobes were structured by electrostatic interactions between environment friendly, negatively charged InP@ZnS quantum dots and positively charged Dured, and then the fluorescence of InP@ZnS QDs/Dured was quenched through fluorescence resonance energy transfer (FRET). At presence of DNA, DNA and Dured were specific binding, which enable Dured removing from the surface of InP@ZnS QDs and achieving fluorescence recovery of InP@ZnS QDs. Thus, the detection of DNA was realized. The linear range of the InP@ZnS QDs/Dured fluorescent nanoprobes for DNA detection and the detection limit were 2.0-275.0 ng·L-1 and 1.0 ng·L-1, respectively. The InP@ZnS QDs/Dured fluorescent nanoprobes can be also used in rapid detection of DNA under simulated physiological conditions. © 2017, Science Press. All right reserved.


Wan X.-L.,Zunyi Medical University | Wan X.-L.,Qiannan Medical College for Nationalities | Lu Y.-F.,Zunyi Medical University | Xu S.-F.,Zunyi Medical University | And 2 more authors.
Biomedicine and Pharmacotherapy | Year: 2017

Oleanolic acid (OA) is a triterpenoid contained in many herbal medicines. The aim of this study was to investigate the protective effect of OA against D-galactosame plus lipopolysaccharides (D-GalN/LPS)-induced acute liver injury and the underling mechanisms. Mice were randomly divided into normal control with vehicles only (corn oil), D-GalN/LPS only (700 mg/10 μg/kg, ip), OA-po (200 μmol/kg in corn oil, po) plus D-GalN/LPS, and OA-sc (50 μmol/kg in 2% tween 80, sc) plus D-GalN/LPS groups. OA pretreatment was conducted twice daily for 4 consecutive days. Hepatotoxicity was evaluated by histopathology, serum enzyme activity, hepatic lipid peroxidation and GSH levels. To reveal the possible mechanisms of the protection, mRNA and protein expressions of toxicity-relevant genes and proteins were examined by real-time RT-PCR and western-blot analysis. Both OA-po and OA-sc at therapeutic doses successfully protected liver injury induced by D-GalN/LPS, as evidenced by reduced serum enzyme activities, prevented liver hemorrhage, massive necrosis, and reduced degenerative lesions. OA increased hepatic GSH contents and decreased lipid peroxidation (MDA) levels. Furthermore, OA significantly inhibited the mRNA expression of the tumor necrosis factor-α (TNF-α) and ER responsive gene Gadd45. D-GalN/LPS-induced activation of p-JNK and NF-κB p65, and protein overexpression of caspase-3, caspase-8, and COX2 were significantly suppressed by OA. These results clearly demonstrated OA-po is effective as OA-sc in protecting against D-GalN/LPS-induced liver injury, and the protection mechanisms are related to reduction of oxidative damage, suppression of TNFα-triggered signaling through the NF-kB and JNK pathways, thus reducing apoptosis and hepatocellular death. © 2017 Elsevier Masson SAS


Zhang L.,Peking Union Medical College | Zhang L.,Qiannan Medical College for Nationalities | Lu S.,Peking Union Medical College
Mini-Reviews in Medicinal Chemistry | Year: 2016

Terpenoids are hydrocarbon compounds derived from common five-carbon isoprene (C5H8) building blocks. They are formed through the condensation and subsequent modification of isoprene units in various ways including-among others-cyclization and/or oxygenation. Their synthesis is localized either to the chloroplast and/or to the cytoplasm/peroxisome/endoplasmic reticulum and mitochondrion. Terpenoids represent a very large and diverse class of metabolites and play important roles in plant growth and development. In addition, they have been intensively used in human health care, disease treatment and in dietary supplements. Approximately 60% of natural products known so far are terpenoids. This review briefly summarizes the biosynthetic pathways of major plant terpenoids. Then, five well-known and medicinally important diterpenoids, including paclitaxel, tanshinone, ginkgolide, triptolide and oridonin are discussed in detail. Their structures, occurrence, extraction and identification methods, pharmacological properties and clinical uses are also reviewed. Finally, the prospects of using biotechnology to produce medicinally important terpenoids are also briefly discussed. © 2016 Bentham Science Publishers.


Wang H.-P.,Peking University | Cao F.,Qiannan Medical College for Nationalities | Yang X.-W.,Peking University
Chinese Traditional and Herbal Drugs | Year: 2013

Objective: To study the chemical constituents in the aerial parts of Polygonum capitatum. Methods: The chemical constituents were isolated and purified by various column chromatographic methods. Their chemical structures were identified by physicochemical properties and spectral analyses. Results: Twenty-five compounds were isolated from the 70% ethanolic extract and identified as {1-[(12E, 16E)-12, 16-eicosadienoyl]-2-[(E, E)-7, 11-octadecadienoyl]-3-stearoylglycerol} (1), 24-methylenecyclo-artenol (2), phytol (3), β-sitosterol (4), kaempferol (5), ethyl protocatechuate (6), p-hydroxybenzoic acid (7), ethyl gallate (8), 5, 7-dihydroxychromone (9), 3-O-methylquercetin (10), gallic acid (11), N-trans-caffeoyltyramine (12), β-daucosterol (13), quercitrin (14), kaempferol-3-O-α-L-rhamnopyranoside (15), kaempferol-3-O-β-D-glucopyranoside (16), 2″-O-galloylquercitrin (17), quercetin (18), 2″-O-galloylhirsutrin (19), myricitrin (20), ethyl brevifolin carboxylate (21), brevifolin (22), quercetin-3-O-[(6″-O-trans- feruloyl)-β-D-galactopyranoside] (23), flazine (24), and ellagic acid (25), respectively. Conclusion: Compounds 1-3, 7, 12, 21-25 are isolated from this plant for the first time.


Zhang R.,Qiannan Medical College for Nationalities | Zhang R.,Chinese Academy of Forestry | Zhang H.,Chinese Academy of Forestry | Zhang Z.,New Zealand Institute for Plant and Food Research | And 3 more authors.
Advanced Materials Research | Year: 2012

Thermal properties of 5 natural resin and wax samples (shellac, rosin, shellac wax, beeswax, Chinese insect wax) were examined by differential scanning calorimetry (DSC). The DSC melting and crystallization curves of the samples were presented in this paper. Three DSC parameters, To, Tf and ΔT (difference between To and Tf), were selected from each curve. Evaluation results of the parameters showed that they were statistically significant with individual excellent reproducibility. Information was provided by evaluation of changes among thermal absorption or release peaks of the curves in differentiating the five resins and waxes. It was demonstrated in this paper that DSC is rapid, convenient, reliable and accurate to qualitatively identify the resins and waxes mentioned above. © (2012) Trans Tech Publications, Switzerland.


Zhang L.,Guizhou University | Zhang L.,Peking Union Medical College | Zhang L.,Qiannan Medical College for Nationalities | Wu B.,Peking Union Medical College | And 4 more authors.
Journal of Integrative Plant Biology | Year: 2014

SQUAMOSA promoter binding protein-likes (SPLs) are plant-specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs in Salvia miltiorrhiza (Danshen), a significant medicinal plant widely used in Traditional Chinese medicine (TCM) for >1,700 years and an emerging model plant for TCM studies. Through genome-wide identification and subsequent molecular cloning, we identified a total 15 SmSPLs with divergent sequence features, gene structures, and motifs. Comparative analysis showed sequence conservation between SmSPLs and their Arabidopsis counterparts. A phylogenetic tree clusters SmSPLs into six groups. Many of the motifs identified commonly exist in a group/subgroup, implying their functional redundancy. Eight SmSPLs were predicted and experimentally validated to be targets of miR156/157. SmSPLs were differentially expressed in various tissues of S. milltiorrhiza. The expression of miR156/157-targeted SmSPLs was increased with the maturation of S. miltiorrhiza, whereas the expression of miR156/157 was decreased, confirming the regulatory roles of miR156/157 in SmSPLs and suggesting the functions of SmSPLs in S. miltiorrhiza development. The expression of miR156/157 was negatively correlated with miR172 during the maturation of S. miltiorrhiza. The results indicate the significance and complexity of SmSPL-, miR156-, and miR172-mediated regulation of developmental timing in S. miltiorrhiza. © 2013 Institute of Botany, Chinese Academy of Sciences.


Hu X.,Nanjing Southeast University | Hu X.,Qiannan Medical College for Nationalities | Zhu K.,Nanjing Southeast University | Guo Q.,Nanjing Southeast University | And 3 more authors.
Analytica Chimica Acta | Year: 2014

This paper reports the construction of a simple CdTe quantum dots (QDs)-based sensor with 1,10-phenanthroline (Phen) as ligand, and the demonstration of a novel ligand displacement-induced fluorescence switch strategy for sensitive and selective detection of Cd2+ in aqueous phase. The complexation of Phen at the surface quenches the green photoluminescence (PL) of QDs dominated by a photoinduced hole transfer (PHT) mechanism. In the presence of Cd2+, the Phen ligands are readily detached from the surface of CdTe QDs, forming [Cd(Phen)2(H2O)2]2+ in solution, and as a consequence the PL of CdTe QDs switches on. The detection limit for Cd2+ is defined as ~0.01nM, which is far below the maximum Cd2+ residue limit of drinking water allowed by the U.S. Environmental Protection Agency (EPA). Two consecutive linear ranges allow a wide determination of Cd2+ from 0.02nM to 0.6μM. Importantly, this CdTe QDs-based sensor features to distinctly discriminate between Cd2+ and Zn2+, and succeeds in real water samples. This extremely simple strategy reported here represents an attempt for the development of fluorescent sensors for ultrasensitive chemo/biodetection. © 2014 Elsevier B.V.


Zheng H.,Chinese Academy of Forestry | Zhang R.,Qiannan Medical College for Nationalities | Zhang R.,Chinese Academy of Forestry | Zhang H.,Chinese Academy of Forestry | And 3 more authors.
Procedia Engineering | Year: 2011

For more wide development of natural wax applied in food additives industry, thermal characteristics of four insect waxes were analyzed by differential scanning calorimetry (DSC), compared with paraffin (an abiological wax) and canauba wax (a vegetal wax). It was indicated that melting point of the four insect waxes were varied at 90.42°C of coccerin as the highest, 70.34°C of beeswax as the lowest, and 86.56°C of insect white wax, 83.82°C of shellac wax. All of the four waxes were higher with the melting point than paraffin (63.32°C), and the coccerin's melting point was even higher than that of canauba wax (87.85°C). The melting enthalpy of insect waxes ranged at 168.1 to 203.2J/g, so did the crystallization enthalpy at 166.9 to 198.8J/g. Both melting and crystallization enthalpies were near to other waxes such as paraffin and canauba wax. It was showed that DSC was qualitatively and quantitatively available for thermal analysis of insect wax, and insect wax was good in thermal stability. © 2011 Published by Elsevier Ltd.


PubMed | Nanjing Medical University, Nanjing University and Qiannan Medical College for Nationalities
Type: Journal Article | Journal: Journal of nanobiotechnology | Year: 2017

Currently, graphene oxide has attracted growing attention as a drug delivery system due to its unique characteristics. Furthermore, utilization of microRNAs as biomarkers and therapeutic strategies would be particularly attractive because of their biological mechanisms and relatively low toxicity. Therefore, we have developed functionalized nanocompounds consisted of graphene oxide, quantum dots and microRNA, which induced cancer cells apoptosis along with targeted imaging.In the present study, we synthesized a kind of graphene-P-gp loaded with miR-122-InP@ZnS quantum dots nanocomposites (GPMQNs) that, in the presence of glutathione, provides controlled release of miR-122. The miR-122 actively targeted liver tumor cells and induced their apoptosis, including drug-resistant liver tumor cells. We also explored the near-infrared fluorescence and potential utility for targeting imaging of InP@ZnS quantum dots. To further understand the molecular mechanism of GPMQNs-induced apoptosis of drug-resistant HepG2/ADM hepatoma cells, the relevant apoptosis proteins and signal pathways were explored in vitro and in vivo. Furthermore, near-infrared GPMQNs, which exhibited reduced photon scattering and auto-fluorescence, were applied for tumor imaging in vivo to allow for deep tissue penetration and three-dimensional imaging.In conclusion, techniques using GPMQNs could provide a novel targeted treatment for liver cancer, which possessed properties of targeted imaging, low toxicity, and controlled release.

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