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Wang H.-P.,Peking University | Cao F.,Qiannan Medical College for Nationalities | Yang X.-W.,Peking University
Chinese Traditional and Herbal Drugs | Year: 2013

Objective: To study the chemical constituents in the aerial parts of Polygonum capitatum. Methods: The chemical constituents were isolated and purified by various column chromatographic methods. Their chemical structures were identified by physicochemical properties and spectral analyses. Results: Twenty-five compounds were isolated from the 70% ethanolic extract and identified as {1-[(12E, 16E)-12, 16-eicosadienoyl]-2-[(E, E)-7, 11-octadecadienoyl]-3-stearoylglycerol} (1), 24-methylenecyclo-artenol (2), phytol (3), β-sitosterol (4), kaempferol (5), ethyl protocatechuate (6), p-hydroxybenzoic acid (7), ethyl gallate (8), 5, 7-dihydroxychromone (9), 3-O-methylquercetin (10), gallic acid (11), N-trans-caffeoyltyramine (12), β-daucosterol (13), quercitrin (14), kaempferol-3-O-α-L-rhamnopyranoside (15), kaempferol-3-O-β-D-glucopyranoside (16), 2″-O-galloylquercitrin (17), quercetin (18), 2″-O-galloylhirsutrin (19), myricitrin (20), ethyl brevifolin carboxylate (21), brevifolin (22), quercetin-3-O-[(6″-O-trans- feruloyl)-β-D-galactopyranoside] (23), flazine (24), and ellagic acid (25), respectively. Conclusion: Compounds 1-3, 7, 12, 21-25 are isolated from this plant for the first time.

Zhang R.,Qiannan Medical College for Nationalities | Zhang R.,Chinese Academy of Forestry | Zhang H.,Chinese Academy of Forestry | Zhang Z.,New Zealand Institute for Plant and Food Research | And 3 more authors.
Advanced Materials Research | Year: 2012

Thermal properties of 5 natural resin and wax samples (shellac, rosin, shellac wax, beeswax, Chinese insect wax) were examined by differential scanning calorimetry (DSC). The DSC melting and crystallization curves of the samples were presented in this paper. Three DSC parameters, To, Tf and ΔT (difference between To and Tf), were selected from each curve. Evaluation results of the parameters showed that they were statistically significant with individual excellent reproducibility. Information was provided by evaluation of changes among thermal absorption or release peaks of the curves in differentiating the five resins and waxes. It was demonstrated in this paper that DSC is rapid, convenient, reliable and accurate to qualitatively identify the resins and waxes mentioned above. © (2012) Trans Tech Publications, Switzerland.

Zhang L.,Guizhou University | Zhang L.,Peking Union Medical College | Zhang L.,Qiannan Medical College for Nationalities | Wu B.,Peking Union Medical College | And 4 more authors.
Journal of Integrative Plant Biology | Year: 2014

SQUAMOSA promoter binding protein-likes (SPLs) are plant-specific transcription factors playing vital regulatory roles in plant growth and development. There is no information about SPLs in Salvia miltiorrhiza (Danshen), a significant medicinal plant widely used in Traditional Chinese medicine (TCM) for >1,700 years and an emerging model plant for TCM studies. Through genome-wide identification and subsequent molecular cloning, we identified a total 15 SmSPLs with divergent sequence features, gene structures, and motifs. Comparative analysis showed sequence conservation between SmSPLs and their Arabidopsis counterparts. A phylogenetic tree clusters SmSPLs into six groups. Many of the motifs identified commonly exist in a group/subgroup, implying their functional redundancy. Eight SmSPLs were predicted and experimentally validated to be targets of miR156/157. SmSPLs were differentially expressed in various tissues of S. milltiorrhiza. The expression of miR156/157-targeted SmSPLs was increased with the maturation of S. miltiorrhiza, whereas the expression of miR156/157 was decreased, confirming the regulatory roles of miR156/157 in SmSPLs and suggesting the functions of SmSPLs in S. miltiorrhiza development. The expression of miR156/157 was negatively correlated with miR172 during the maturation of S. miltiorrhiza. The results indicate the significance and complexity of SmSPL-, miR156-, and miR172-mediated regulation of developmental timing in S. miltiorrhiza. © 2013 Institute of Botany, Chinese Academy of Sciences.

Hu X.,Nanjing Southeast University | Hu X.,Qiannan Medical College for Nationalities | Zhu K.,Nanjing Southeast University | Guo Q.,Nanjing Southeast University | And 3 more authors.
Analytica Chimica Acta | Year: 2014

This paper reports the construction of a simple CdTe quantum dots (QDs)-based sensor with 1,10-phenanthroline (Phen) as ligand, and the demonstration of a novel ligand displacement-induced fluorescence switch strategy for sensitive and selective detection of Cd2+ in aqueous phase. The complexation of Phen at the surface quenches the green photoluminescence (PL) of QDs dominated by a photoinduced hole transfer (PHT) mechanism. In the presence of Cd2+, the Phen ligands are readily detached from the surface of CdTe QDs, forming [Cd(Phen)2(H2O)2]2+ in solution, and as a consequence the PL of CdTe QDs switches on. The detection limit for Cd2+ is defined as ~0.01nM, which is far below the maximum Cd2+ residue limit of drinking water allowed by the U.S. Environmental Protection Agency (EPA). Two consecutive linear ranges allow a wide determination of Cd2+ from 0.02nM to 0.6μM. Importantly, this CdTe QDs-based sensor features to distinctly discriminate between Cd2+ and Zn2+, and succeeds in real water samples. This extremely simple strategy reported here represents an attempt for the development of fluorescent sensors for ultrasensitive chemo/biodetection. © 2014 Elsevier B.V.

Ma Y.-M.,Peking Union Medical College | Yuan L.-C.,Peking Union Medical College | Zhang L.-S.,Peking Union Medical College | Zhang L.-S.,Qiannan Medical College for Nationalities | And 3 more authors.
Chinese Traditional and Herbal Drugs | Year: 2014

Objective: To clone and identify the squalene synthase genes in Salvia miltiorrhiza. Methods: The primers were designed based on the predication result of S. miltiorrhiza genome genes and two squalene synthase genes (SmSQS1 and SmSQS2) from S. miltiorrhiza were amplified via RT-PCR method. Some bioinformatic methods and softwares were used for the gene structure analysis, the analyses of sequence homology and protein conserved domains of the two gene encoding polypeptides were carried. Real-time quantitative PCR (RT-qPCR) method was used for the analysis of gene expression patterns. Results: The two squalene synthase genes of S. miltiorrhiza were obtained by RT-PCR method. The encoded polypeptide of the two genes has the related domains and a conserved motif for squalene synthases. The two genes had the different exon/intron characteristics and the different tissue-specific and time-specific expression patterns. Conclusion: There are two squalene synthase genes existing in the genome of S. miltiorrhiza, which have the different gene structures and expression patterns, and probably play the different roles in the biosynthesis process of steroids and triterpenoids in S. miltiorrhiza.

Huang B.,Nanjing Medical University | Liu H.,Nanjing Medical University | Huang D.,Nanjing Changjiang Hospital | Mao X.,Yixing Peoples Hospital | And 5 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2016

Wogonin (5,7-dihydroxy-8-methoxyflavone) is one of the active components of flavonoids isolated from Scutellariae radix and possesses antitumor effect against leukemia. Cadmium-telluride quantum dots (CdTe-QDs) are a kind of nanoparticles with great potential in functioning as an efficient drug delivery vector in biomedical research. In this study, we investigated the synergistic effect of CdTe-QDs with Wogonin on the induction of apoptosis using drug-resistant human leukemia KA cells. Flow cytometry analysis, assay of morphology under electron microscope, quantitative analysis of tumor volume and micro-CT imaging demonstrated that compared with that by pure CdTe-QDs or wogonin, the apoptosis rate increased sharply when treated wirh CdTe-QDs together with wogonin on KA cells. These results proved that the nanocomposites readily overcame the barrier of drug-resistance and provoked cell apoptosis in vitro and in vivo by facilitating the interaction between wogonin and KA cells. As known to all, it is an inevitable tendency that new effective therapies will take the place of conventional chemotherapy and radiotherapy presenting significant disadvantages. According to this article, CdTe-QD combined with wogonin is a possible alternative for some cancer treatments. Copyright © 2016 American Scientific Publishers. All rights reserved.

Zheng H.,Chinese Academy of Forestry | Zhang R.,Qiannan Medical College for Nationalities | Zhang R.,Chinese Academy of Forestry | Zhang H.,Chinese Academy of Forestry | And 3 more authors.
Procedia Engineering | Year: 2011

For more wide development of natural wax applied in food additives industry, thermal characteristics of four insect waxes were analyzed by differential scanning calorimetry (DSC), compared with paraffin (an abiological wax) and canauba wax (a vegetal wax). It was indicated that melting point of the four insect waxes were varied at 90.42°C of coccerin as the highest, 70.34°C of beeswax as the lowest, and 86.56°C of insect white wax, 83.82°C of shellac wax. All of the four waxes were higher with the melting point than paraffin (63.32°C), and the coccerin's melting point was even higher than that of canauba wax (87.85°C). The melting enthalpy of insect waxes ranged at 168.1 to 203.2J/g, so did the crystallization enthalpy at 166.9 to 198.8J/g. Both melting and crystallization enthalpies were near to other waxes such as paraffin and canauba wax. It was showed that DSC was qualitatively and quantitatively available for thermal analysis of insect wax, and insect wax was good in thermal stability. © 2011 Published by Elsevier Ltd.

Li K.,Chinese Academy of Forestry | Zhang R.,Qiannan Medical College for Nationalities | Zhang H.,Chinese Academy of Forestry | Zheng H.,Chinese Academy of Forestry | Zhang W.,Chinese Academy of Forestry
Advanced Materials Research | Year: 2012

For the wide application of insect wax, it was studied on its thermal properties under different conditions in which the wax was stored at cold, dark, indoor and sunshine environment. Differential scanning calorimetry (DSC) was selected to achieve thermal parameters and reveal the influence of temperature on the stability of the wax. The result showed that within 20 months of natural storage, it was significant influence on the melting enthalpy of Hunan insect wax, the melting point of Sichuan insect wax and the melting peak of Sichuan insect wax with the Sig values were 0.013, 0.009 and 0.000. and the quality of wax was characterized by IR, XRD and physical-chemical indexes. The results showed that within 20 months, it was slightly changed in the crystallinity and chemical constituents. Therefore, the wax with good stability can be used for medicine, food, functional materials and other industrial purposes safely. © (2012) Trans Tech Publications, Switzerland.

Long Y.-G.,Qiannan Medical College for Nationalities | Yu Y.-S.,Qiannan Medical College for Nationalites | Long S.-F.,Qiannan Medical College for Nationalities | Jiang J.-Z.,Qiannan Medical College for Nationalities
Acta Anatomica Sinica | Year: 2010

Objective: To investigate genetic polymorphism of 3 STR loci in Bouyei ethnic group in Guizhou. Methods: The DNAs of 101 healthy unrelated Bouyei individuals in Guizhou were extracted using chelex-100 method and were multiplex amplificated by PCR technique and the denatured PAGE was used, the PCR product was typied using silver stain method. The data was statistically analysed by modified-powerstat software package. Results: A total of 21 alleles and 50 genotypes were observed in the 3 loci and the distributions of these genotypes were consistent with the law of Hardy-Weinberg equilibrium. The heterozygosity (H) of CSFlPO, TPOX and THO1 loci were 0.7287, 0.5423 and 0.6904; the polymorphism information content (PIC) were 0.6805, 0.4479 and 0.6426; the discrimination power (DP) were 0.8782, 0.7361 and 0.8563 and the probabilities of paternity exclusion (PPE) were 0.6148, 0.3643 and 0.5737 respectively. Conclution: CSFlPO and THOX are highly genetic polymolphism, which is valuable for population genetic research and forensic individual identification. The distrbution of gene frequencies and their area of ethnic groups have parallel relation.

PubMed | Nanjing Medical University, Nanjing University and Qiannan Medical College for Nationalities
Type: Journal Article | Journal: Journal of nanobiotechnology | Year: 2017

Currently, graphene oxide has attracted growing attention as a drug delivery system due to its unique characteristics. Furthermore, utilization of microRNAs as biomarkers and therapeutic strategies would be particularly attractive because of their biological mechanisms and relatively low toxicity. Therefore, we have developed functionalized nanocompounds consisted of graphene oxide, quantum dots and microRNA, which induced cancer cells apoptosis along with targeted imaging.In the present study, we synthesized a kind of graphene-P-gp loaded with miR-122-InP@ZnS quantum dots nanocomposites (GPMQNs) that, in the presence of glutathione, provides controlled release of miR-122. The miR-122 actively targeted liver tumor cells and induced their apoptosis, including drug-resistant liver tumor cells. We also explored the near-infrared fluorescence and potential utility for targeting imaging of InP@ZnS quantum dots. To further understand the molecular mechanism of GPMQNs-induced apoptosis of drug-resistant HepG2/ADM hepatoma cells, the relevant apoptosis proteins and signal pathways were explored in vitro and in vivo. Furthermore, near-infrared GPMQNs, which exhibited reduced photon scattering and auto-fluorescence, were applied for tumor imaging in vivo to allow for deep tissue penetration and three-dimensional imaging.In conclusion, techniques using GPMQNs could provide a novel targeted treatment for liver cancer, which possessed properties of targeted imaging, low toxicity, and controlled release.

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