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Wang Y.,Southern Medical University | He D.,Central University of Costa Rica | Yang L.,Qianjiang Central Hospital | Wen B.,Southern Medical University | And 6 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

Hepatocellular carcinoma (HCC) is the one of the most common malignancies worldwide and its prognosis is extremely poor. Tripartite motif (TRIM) proteins play crucial roles in cancer cell biology but the function of tripartite motif 26 (TRIM26) has not been investigated. We demonstrated that low expression level of TRIM26 in tumor samples was significantly correlated with worse prognosis in HCC patients. We also demonstrated its expression level was associated with several clinicopathologic features such as AFP level and T stage of HCC patients. Furthermore, we validated that TRIM26 was significantly downregulated in HCC tissue compared with normal liver tissue. To further clarify the functional role of TRIM26 in HCC, We confirmed that TRIM26 silencing can promote cancer cell proliferation, colony forming, migration and invasion in vitro with HCC cell lines HepG2 and Bel-7402. Then we utilized bioinformatic tool to predict gene influenced by TRIM26, showing TRIM26 could modulate gene sets about cancer cell metabolism. In conclusion, we proved that TRIM26 is a novel tumor suppressor modulating multiple metabolism-related pathways in HCC. To our best knowledge, this is the first study to investigate the function of TRIM26 in cancer biology. Our findings provide useful insight into the mechanism of HCC origin and progression. Moreover, TRIM26 may represent a novel therapeutic target for HCC. © 2015 Elsevier Inc. All rights reserved.

Zhang K.,Chongqing Medical University | Qin H.,Chongqing Medical University | Pan F.,Chongqing Medical University | Liu E.,Qianjiang Central Hospital | And 2 more authors.
Medical Science Monitor | Year: 2014

Material/Methods: We retrospectively reviewed patients with stage III-IV unresectable NSCLC from 1 January 2010 to 31 December 2013 at Southwest Hospital. They all received nedaplatin (80 mg/m2, nedaplatin group) or oxaliplatin (130 mg/m2, oxaliplatin group) combined with paclitaxel (175 mg/m2) or docetaxel (75 mg/m2) as first-line treatment.Results: There are 174 patients enrolled – 123 patients in the nedaplatin group and 51 patients in the oxaliplatin group. The objective response rates were 47.3% and 34.1% and the disease control rates were 87.5% and 79.5% in nedaplatin and oxaliplatin groups, respectively. The progression-free survival time was 10.4 months and 9.6 months (p=0.722) and the overall survival time was 18.5 months and 25.5 months in the nedaplatin and ox-aliplatin groups, respectively (p=0.09). Total toxicity was greater in the oxaliplatin group (p=0.008), but there is no significant difference among ¾ grade adverse events between the 2 groups (P=0.595).Conclusions: The effect of nedaplatin plus paclitaxel and docetaxel is the same as oxaliplatin plus paclitaxel and docetaxel, and the toxicity of nedaplatin is well tolerate as first-line treatment for patients with advanced NSCLC.Background: Both nedaplatin and oxaliplatin combined with paclitaxel or docetaxel have demonstrated potent activity in advanced non-small cell lung cancer (NSCLC) patients, but there is no study comparing the difference between these 2 chemotherapy regimens. The aim of this study was to evaluate and compare the efficacy and safety between the combination chemotherapy of nedaplatin or oxaliplatin plus paclitaxel and docetaxel in patients with advanced NSCLC. © Med Sci Monit.

Long Z.-Q.,Qianjiang Central Hospital
International Eye Science | Year: 2013

AIM: To observe the clinical effect and recurrence rate of biological amnion transplantation in pterygium operation, and to provide the reference for pterygium diagnosis. METHODS: Fifty-two patients (58 eyes) with pterygium were conducted microscope excision combined with biological amnion transplantation from April, 2010 to September, 2012. The repair time of corneal epithelium and postoperative recurrence rate were recorded. Forty-six patients (53 eyes) receiving excision of pterygium with microscope alone at the same term were selected to make a controlled study. RESULTS: The recurrence rate of patients in research group was significantly lower than that in control group. The proportion of healing cases in research group on the 3rd and 5th days after operation were remarkably higher than that in control group, while that on the 14th day conspicuously lower than that in control group. The mean healing days of patients in research group were markedly shorter than that in control group. In addition, the patient comfort of the research group was significantly higher than that in control group. CONCLUSION: Excision of pterygium with microscope combined with biological amnion transplantation can effectively shorten the postoperative repair time of corneal epithelium, decrease the recurrence rate distinctly, and is more comfortable, so it deserves to be recommended.

Zhang Y.,Hubei University of Chinese Medicine | Chen R.,Qianjiang Central Hospital | Xu L.,Hubei University of Chinese Medicine | Ning Y.,Hubei University of Chinese Medicine | And 2 more authors.
Analytical Sciences | Year: 2015

Silicon nanowire (SiNW) field-effect transistor (FET) biosensors have already been used as powerful sensors for the direct detection of disease-related biomarkers. However, the multiplexed detection of biomarkers in real samples is still challenging. Interleukin 8 (IL-8) and tumor necrosis factor α (TNF-α) are two typical biomarkers of oral squamous cell carcinoma (OSCC). In this study, we developed a multiplexed detection methodology for IL-8 and TNF-α detection in saliva using SiNW FET biosensors. We fabricated the SiNW FET sensors using a top-down lithography fabrication technique. Subsequently, we achieved the multiplexed detection of two biomarkers in saliva by specific recognition of the two biomarkers with their corresponding antibodies, which were modified on the SiNW. The established method was found to have a limit of detection as low as 10 fg/mL in 1×PBS as well as 100 fg/mL in artificial saliva. Because of its advantages, including label-free and multiplexed detection, non-invasive analysis, highly sensitive and specific determination, the proposed method is expected to be widely used for the early diagnosis of OSCC. © The Japan Society for Analytical Chemistry.

Wu T.,China Institute of Technology | Huang Y.,Qianjiang Central Hospital | Zhu Y.,Wuhan University | Ruan Q.,Jingmen Rehabilitation Hospital | And 2 more authors.
Medical Journal of Wuhan University | Year: 2014

Objective: To estimate the blocking efficacy of 3 kinds of root canal filling materials at various section and to analyze the influence factors. Methods: Sixty freshly extracted m andibular first premolar with single root were collected. The samples were r andomly divided into A, B, and C groups, with 20 teeth in each group. The teeth in A, B, and C groups were respectively filled with gutta-percha point of the same kind plus Zinc Oxide-eugenol (ZOE) paste, Vitapex paste and AH-Plus paste in lateral pressure way. Then roots were sectioned at 2, 4, 6, and 8mm levels respectively from apical foramen. The binding state between filling materials and the root canal wall and their micro-chink were measured under scanning electron microscope (SEM). Results: Under SEM, gaps between different filling materials and root canal wall were all observed, but the binding state of AH-Plus paste group was obviously better than that of the other two groups. At the 2 mm level from apex, the micro-chink of AH-Plus paste group ([2.25±7.63] μm) was significantly lower than that of ZOE paste group ([9.56±3.12] μm) and that of Vitapex paste group ([8.81±1.55] μm) (P<0.05). At the 4, 6 and 8 mm levels, there were no significant differences in micro-chink among these three groups (P>0.05). Conclusion: AH-Plus paste, used as root canal filling material, showed better sealing ability, and it could effectively prevent the occurrence of apical microleakage.

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