Qianjiang Central Hospital

Yuanlin, China

Qianjiang Central Hospital

Yuanlin, China

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Wang Z.,Renmin University of China | Chen A.,Qianjiang Central Hospital | Yan S.,Qianjiang Central Hospital | Li C.,Renmin University of China
Cell Biochemistry and Function | Year: 2016

The aim of this study was to explore the curative effect of differentiated human umbilical cord–derived mesenchymal stem cells (hUC-MSCs) transplantation on rat of advanced Parkinson disease (PD) model. Human umbilical cord–derived mesenchymal stem cells were cultured and induced differentiation in vitro. The PD rats were established and allocated randomly into 2 groups: differentiated hUC-MSCs groups and physiological saline groups (the control group). Rotation test and immunofluorescence double staining were done. The result showed that hUC-MSCs could differentiate into mature dopamine neurons. Frequency of rotation was significantly less in differentiated hUC-MSCs groups than in normal saline group. After we transplanted these cells into the unilateral lesioned substantia nigra induced by striatal injection of 6-hydroxydopamine and performed in the medial forebrain bundle and ventral tegmental area, nigral tyrosine hydroxylase–positive cells were observed and survival of at least 2 months. In addition, transplantation of hUC-MSCs could make an obviously therapeutic effect on PD rats. Copyright © 2016 John Wiley & Sons, Ltd.


Xia Z.-P.,Wuhan University | Zheng X.-M.,Wuhan University | Zheng H.,Wuhan University | Liu X.-J.,Wuhan University | And 2 more authors.
Asian Journal of Andrology | Year: 2012

Cold-inducible RNA-binding protein (CIRP) is an RNA-binding protein that is expressed in normal testes and downregulated after heat stress caused by cryptorchidism, varicocele or environmental temperatures. The purpose of this study was to investigate the functions of CIRP in the testes. We employed RNAi technique to knock down the expression of CIRP in the testes, and performed haematoxylin and eosin staining to evaluate morphological changes following knockdown. Germ cell apoptosis was examined by terminal deoxynucleotidal transferase-mediated dUTP nick end labelling (TUNEL) assay, and mitogen-activated protein kinase (MAPK) signalling pathways were investigated by Western blotting to determine the possible mechanism of apoptosis. We found that using siRNA is a feasible and reliable method for knocking down gene expression in the testes. Compared to controls, the mean seminiferous tubule diameter (MSTD) and the thickness of the germ cell layers decreased following siRNA treatment, whereas the percentage of apoptotic seminiferous tubules increased. The p44/p42, p38 and SAPK/JNK MAPK pathways were activated after downregulation of CIRP. In conclusion, we discovered that downregulation of CIRP resulted in increased germ cell apoptosis, possibly via the activation of the p44/p42, p38 and SAPK/JNK MAPK pathways. © 2012 AJA, SIMM & SJTU. All rights reserved.


Li T.-B.,Qianjiang Central Hospital | Zhang H.-Q.,Qianjiang Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Previously, many authors advocated that the rib fractures can be treated by conservative method. However, with the progress of internal fixation materials science and in-depth study of the rib injury, many authors have begun to recommend that rib fractures should be treated by internal fixation surgery, which may shorten the treatment period and improve patient’s quality of life. But which is the optimal fixation methods, is still controversial. OBJECTIVE: To summarize the clinical outcomes of the memory alloy osteosynthesis plate fixation for flail chest injuries. METHODS: From May 2008 to May 2011, 50 patients with flail chest were treated with memory alloy osteosynthesis plate fixation in Department of Thoracic Surgery, Qianjiang Central Hospital. There are 39 males and 11 females, the mean age was 42.6 years (range 22-67 years). The flail chest injuries were caused by traffic accidents in 38 cases, by falling from height in 11 cases, and by tire blast injury in 1 case. The mean number of rib fractures was 6.4±4.3 (range 3-11); the mean fracture sites were 11.2±5.6 (range 7-23). There were 25 patients with flail chest at left side, 17 cases at right side, and 8 cases at bilateral sides. The mean time from injury to the surgical time was 3.7±2.2 days (range 2-7 days). The length of postoperative ventilator use, postoperative ICU days, length of hospital stay, peripheral complications, fracture reduction and fracture healing time were observed. The chest wall pains before and after surgery were evaluated with the visual analog scale. RESULTS AND CONCLUSION: All incisions obtained healing by first intention and all patients were followed up for 13-22 months. There were no intraoperative complications. Mean postoperative ventilator days were 1.8±0.7 days (range 1-3 days); mean post-operative ICU days were 2.6±1.1 days (range 2-5 days); mean lengths of hospital stay were 14.9±3.1 days (range 12-17 days); the visual analog scale scores was reduced from preoperative 8.1±1.2 points to 4.9±0.9 points at 1 day post-surgery and 3.2±1.1 points at 1 week post-surgery. No cases of hardware failure, hardware prominence, wound infection, or nonunion were observed. All fractures reached clinical healing, and mean healing time was 3.1±1.2 months (range 2-4 months). The memory alloy osteosynthesis plate fixation is an effective technique for the treatment of flail chest, can provide stability for the injured segments of the chest wall, reduce ventilator time, decrease ventilator-associated complications, reduce deformity, and decrease chronic pain. © 2015, Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved.


Wang Y.,Southern Medical University | He D.,Central University of Costa Rica | Yang L.,Qianjiang Central Hospital | Wen B.,Southern Medical University | And 6 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

Hepatocellular carcinoma (HCC) is the one of the most common malignancies worldwide and its prognosis is extremely poor. Tripartite motif (TRIM) proteins play crucial roles in cancer cell biology but the function of tripartite motif 26 (TRIM26) has not been investigated. We demonstrated that low expression level of TRIM26 in tumor samples was significantly correlated with worse prognosis in HCC patients. We also demonstrated its expression level was associated with several clinicopathologic features such as AFP level and T stage of HCC patients. Furthermore, we validated that TRIM26 was significantly downregulated in HCC tissue compared with normal liver tissue. To further clarify the functional role of TRIM26 in HCC, We confirmed that TRIM26 silencing can promote cancer cell proliferation, colony forming, migration and invasion in vitro with HCC cell lines HepG2 and Bel-7402. Then we utilized bioinformatic tool to predict gene influenced by TRIM26, showing TRIM26 could modulate gene sets about cancer cell metabolism. In conclusion, we proved that TRIM26 is a novel tumor suppressor modulating multiple metabolism-related pathways in HCC. To our best knowledge, this is the first study to investigate the function of TRIM26 in cancer biology. Our findings provide useful insight into the mechanism of HCC origin and progression. Moreover, TRIM26 may represent a novel therapeutic target for HCC. © 2015 Elsevier Inc. All rights reserved.


Zhang K.,Chongqing Medical University | Qin H.,Chongqing Medical University | Pan F.,Chongqing Medical University | Liu E.,Qianjiang Central Hospital | And 2 more authors.
Medical Science Monitor | Year: 2014

Material/Methods: We retrospectively reviewed patients with stage III-IV unresectable NSCLC from 1 January 2010 to 31 December 2013 at Southwest Hospital. They all received nedaplatin (80 mg/m2, nedaplatin group) or oxaliplatin (130 mg/m2, oxaliplatin group) combined with paclitaxel (175 mg/m2) or docetaxel (75 mg/m2) as first-line treatment.Results: There are 174 patients enrolled – 123 patients in the nedaplatin group and 51 patients in the oxaliplatin group. The objective response rates were 47.3% and 34.1% and the disease control rates were 87.5% and 79.5% in nedaplatin and oxaliplatin groups, respectively. The progression-free survival time was 10.4 months and 9.6 months (p=0.722) and the overall survival time was 18.5 months and 25.5 months in the nedaplatin and ox-aliplatin groups, respectively (p=0.09). Total toxicity was greater in the oxaliplatin group (p=0.008), but there is no significant difference among ¾ grade adverse events between the 2 groups (P=0.595).Conclusions: The effect of nedaplatin plus paclitaxel and docetaxel is the same as oxaliplatin plus paclitaxel and docetaxel, and the toxicity of nedaplatin is well tolerate as first-line treatment for patients with advanced NSCLC.Background: Both nedaplatin and oxaliplatin combined with paclitaxel or docetaxel have demonstrated potent activity in advanced non-small cell lung cancer (NSCLC) patients, but there is no study comparing the difference between these 2 chemotherapy regimens. The aim of this study was to evaluate and compare the efficacy and safety between the combination chemotherapy of nedaplatin or oxaliplatin plus paclitaxel and docetaxel in patients with advanced NSCLC. © Med Sci Monit.


Zhang Y.,Hubei University of Chinese Medicine | Chen R.,Qianjiang Central Hospital | Xu L.,Hubei University of Chinese Medicine | Ning Y.,Hubei University of Chinese Medicine | And 2 more authors.
Analytical Sciences | Year: 2015

Silicon nanowire (SiNW) field-effect transistor (FET) biosensors have already been used as powerful sensors for the direct detection of disease-related biomarkers. However, the multiplexed detection of biomarkers in real samples is still challenging. Interleukin 8 (IL-8) and tumor necrosis factor α (TNF-α) are two typical biomarkers of oral squamous cell carcinoma (OSCC). In this study, we developed a multiplexed detection methodology for IL-8 and TNF-α detection in saliva using SiNW FET biosensors. We fabricated the SiNW FET sensors using a top-down lithography fabrication technique. Subsequently, we achieved the multiplexed detection of two biomarkers in saliva by specific recognition of the two biomarkers with their corresponding antibodies, which were modified on the SiNW. The established method was found to have a limit of detection as low as 10 fg/mL in 1×PBS as well as 100 fg/mL in artificial saliva. Because of its advantages, including label-free and multiplexed detection, non-invasive analysis, highly sensitive and specific determination, the proposed method is expected to be widely used for the early diagnosis of OSCC. © The Japan Society for Analytical Chemistry.


Long Z.-Q.,Qianjiang Central Hospital
International Eye Science | Year: 2013

AIM: To observe the clinical effect and recurrence rate of biological amnion transplantation in pterygium operation, and to provide the reference for pterygium diagnosis. METHODS: Fifty-two patients (58 eyes) with pterygium were conducted microscope excision combined with biological amnion transplantation from April, 2010 to September, 2012. The repair time of corneal epithelium and postoperative recurrence rate were recorded. Forty-six patients (53 eyes) receiving excision of pterygium with microscope alone at the same term were selected to make a controlled study. RESULTS: The recurrence rate of patients in research group was significantly lower than that in control group. The proportion of healing cases in research group on the 3rd and 5th days after operation were remarkably higher than that in control group, while that on the 14th day conspicuously lower than that in control group. The mean healing days of patients in research group were markedly shorter than that in control group. In addition, the patient comfort of the research group was significantly higher than that in control group. CONCLUSION: Excision of pterygium with microscope combined with biological amnion transplantation can effectively shorten the postoperative repair time of corneal epithelium, decrease the recurrence rate distinctly, and is more comfortable, so it deserves to be recommended.


PubMed | Hubei University and Qianjiang Central Hospital
Type: Journal Article | Journal: Cell biochemistry and function | Year: 2016

The aim of this study was to explore the curative effect of differentiated human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) transplantation on rat of advanced Parkinson disease (PD) model. Human umbilical cord-derived mesenchymal stem cells were cultured and induced differentiation in vitro. The PD rats were established and allocated randomly into 2 groups: differentiated hUC-MSCs groups and physiological saline groups (the control group). Rotation test and immunofluorescence double staining were done. The result showed that hUC-MSCs could differentiate into mature dopamine neurons. Frequency of rotation was significantly less in differentiated hUC-MSCs groups than in normal saline group. After we transplanted these cells into the unilateral lesioned substantia nigra induced by striatal injection of 6-hydroxydopamine and performed in the medial forebrain bundle and ventral tegmental area, nigral tyrosine hydroxylase-positive cells were observed and survival of at least 2months. In addition, transplantation of hUC-MSCs could make an obviously therapeutic effect on PD rats.


PubMed | Hubei University of Medicine, Qianjiang Central Hospital, Renmin University of China, Southern Medical University and Central University of Costa Rica
Type: Journal Article | Journal: Biochemical and biophysical research communications | Year: 2015

Hepatocellular carcinoma (HCC) is the one of the most common malignancies worldwide and its prognosis is extremely poor. Tripartite motif (TRIM) proteins play crucial roles in cancer cell biology but the function of tripartite motif 26 (TRIM26) has not been investigated. We demonstrated that low expression level of TRIM26 in tumor samples was significantly correlated with worse prognosis in HCC patients. We also demonstrated its expression level was associated with several clinicopathologic features such as AFP level and T stage of HCC patients. Furthermore, we validated that TRIM26 was significantly downregulated in HCC tissue compared with normal liver tissue. To further clarify the functional role of TRIM26 in HCC, We confirmed that TRIM26 silencing can promote cancer cell proliferation, colony forming, migration and invasion in vitro with HCC cell lines HepG2 and Bel-7402. Then we utilized bioinformatic tool to predict gene influenced by TRIM26, showing TRIM26 could modulate gene sets about cancer cell metabolism. In conclusion, we proved that TRIM26 is a novel tumor suppressor modulating multiple metabolism-related pathways in HCC. To our best knowledge, this is the first study to investigate the function of TRIM26 in cancer biology. Our findings provide useful insight into the mechanism of HCC origin and progression. Moreover, TRIM26 may represent a novel therapeutic target for HCC.


PubMed | Qianjiang central Hospital
Type: Journal Article | Journal: Acta cirurgica brasileira | Year: 2016

To investigate the protective effects of dexmedetomidine (Dex) against renal ischemia/reperfusion injury (IRI).Sprague-Dawley rats were randomly divided to sham group, IRI group and Dex group. The SD rats were subjected to 45 min of ischemia followed by eight weeks of reperfusion. Prior to ischemia, rats were either treated with Dex or not. Blood samples were collected for the detection of blood urea nitrogen (BUN) and creatinine (Cr) levels. Immunohistochemistry was performed for CD3 T-cell infiltrates. Real-time PCR and western blot were detected for the expression of TNF-, IL-1, ICAM-1, HMGB1 and TLR4.Compared with sham group, renal IRI significantly increased the serum levels of BUN and Cr. The H&E staining indicated that renal IRI resulted in obvious renal injury and immunohistochemistry found that there were more CD3 T-cell infiltrates in IRI group. Also, renal IRI upregulated the expression of TNF-, IL-1, ICAM-1, HMGB1 and TLR4. However, all these changes were alleviated by the treatment with Dex.Dexmedetomidine has beneficial effects on long term inflammation induced by renal ischemia/reperfusion injury. Its mechanisms may be achieved through inhibiting the HMGB1/TLR4 pathway to exert protective effects.

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