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Wang D.,Qianfoshan Hospital of Shandong Province | Yuan X.,Lanzhou University | Liu T.,Shihezi University | Liu L.,Shihezi University | And 5 more authors.
Molecules | Year: 2012

The air-dried aerial parts of Lavandula angustifolia Mill, a traditional Uygur herbal drug, is used as resuscitation-inducing therapy to treat neurodisfunctions, such as stroke. This study was designed to assess the neuroprotective effects of lavender oil against ischemia/reperfusion (IR) injury in mice. Focal cerebral ischemia was induced by the intraluminal occlusion method with a nylon string. The neurodysfuntion was evaluated by neurological deficit and the infarct area was showed by 2,3,5-triphenyltetrazolium chloride (TTC) staining. The histopathological changes were observed by hematoxylin and eosin staining. The levels of mitochondria-generated reactive oxygen species (ROS), malondialdehyde (MDA) and carbonyl, the ratio of reduced glutathione (GSH)/glutathione disulfide (GSSG), the activities of superoxide dismutase (SOD), catalase (CAT) and glutathion peroxidase (GSH-Px) in brain tissue were measured to estimate the oxidative stress state. Neurological deficit, infarct size, histopathology changes and oxidative stress markers were evaluated after 22 h of reperfusion. In comparison with the model group, treatment with lavender oil significantly decreased neurological deficit scores, infarct size, the levels of MDA, carbonyl and ROS, and attenuated neuronal damage, upregulated SOD, CAT, GSH-Px activities and GSH/GSSG ratio. These results suggested that the neuroprotective effects of lavender oil against cerebral ischemia/reperfusion injury may be attributed to its antioxidant effects. Source


Wu M.,Shandong University | Shao G.,Shandong University | Zhang F.,Shandong University | Ruan Z.,Shandong University | And 2 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2015

Purpose: This study was to evaluate the accuracy and clinical application of a new technique in the fetal weight estimation by ultrasound. Methods: The fetal biparietal diameter (BPD), head circumference (HC) and femur length (FL) were measured by ultrasound examination within one week before delivery. Two different fetal abdominal sectors were measured for the assessment of abdominal circumference (AC). The fetal weight of 516 singleton fetuses was estimated according to above measurements and their actual birth weight was recorded after delivery. Results: There were no significant differences in the fetal AC or body weight evaluated before delivery and recorded after delivery. Conclusion: This new technique is more convenient and applicable for the evaluation of fetal AC as compared to standard method, and seems to be reliable and accurate for the assessment of fetal weight. © 2015, E-Century Publishing Corporation. All rights reserved. Source


Fang W.-T.,Nanjing Medical University | Li H.-J.,Qianfoshan Hospital of Shandong Province | Zhang H.,Nanjing Medical University | Jiang S.,Nanjing Medical University
British Journal of Clinical Pharmacology | Year: 2012

AIMS The use of statins has been suggested to protect against atrial fibrillation (AF) in some clinical observational and experimental studies but has remained inadequately explored. This study was designed to examine whether statins can reduce the risk of AF. METHODS Meta-analysis of randomized, controlled trials with use of statins on incidence or recurrence of AF was performed. RESULTS Twenty studies with 23577 patients were included in the analysis. Seven studies investigated the use of statins in patients with AF, 11 studies investigated the primary prevention of statins in patients without AF, and two studies investigated mixed populations of patients. The incidence or recurrence of AF occurred in 1543 patients. Overall, statin therapy was significantly associated with a decreased risk of AF compared with control (odds ratio 0.49, 95% confidence interval 0.37-0.65; P<0.00001). A beneficial effect was found in the atorvastatin subgroup and the simvastatin subgroup, but not in the pravastatin subgroup or the rosuvastatin subgroup. The benefit of statin therapy appeared to be more pronounced in secondary prevention (odds ratio 0.34, 95% confidence interval 0.18-0.64; P<0.0008) than in primary prevention (odds ratio 0.54, 95% confidence interval 0.40-0.74; P<0.0001). CONCLUSIONS Statin therapy was significantly associated with a decreased risk of incidence or recurrence of AF. Heterogeneity was explained by differences in statin types, patient populations and surgery types. The benefit of statin therapy seemed more pronounced in secondary than in primary prevention. © 2012 The Authors. British Journal of Clinical Pharmacology © 2012 The British Pharmacological Society. Source


Han J.,Shihezi University | Wang D.,Qianfoshan Hospital of Shandong Province | Yu B.,Shihezi University | Wang Y.,Shihezi University | And 4 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2014

The generation of reactive oxygen species (ROS) is a major cause of heart injury induced by ischemia-reperfusion. The left ventricular developed pressure (LVDP) and the maximumup/down rate of left ventricular pressure (±dp/dtmax) were documented by a physiological recorder. Myocardial infarct size was estimated macroscopically using 2,3,5-triphenyltetrazolium chloride staining. Coronary effluent was analyzed for lactate dehydrogenase (LDH) and creatine kinase (CK) release to assess the degree of cardiac injury. The levels of C-reactive protein (CRP), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) were analyzed to determine the inflammation status of the myocardial tissue. Cardiomyocyte apoptosis analysis was performed using the In Situ Cell Death Detection Kit, POD. Accordingly, licochalcone B pretreatment improved the heart rate (HR), increased LVDP, and decreased CK and LDH levels in coronary flow. SOD level and GSH/GSSG ratio increased, whereas the levels of MDA, TNF-α, and CRP and activities of IL-8 and IL-6 decreased in licochalcone B-treated groups. The infarct size and cell apoptosis in hearts from licochalcone B-treated groupwere lower than those in hearts from the I/R control group. Therefore, the cardioprotective effects of licochalcone B may be attributed to its antioxidant, antiapoptotic, and anti-inflammatory activities. Copyright © 2014 Jichun Han et al. Source


Ma J.,Binzhou Medical University | Ma J.,Yantai University | Yuan X.,Shihezi University | Qu H.,Binzhou Medical University | And 5 more authors.
Life Sciences | Year: 2015

Aims The alteration of ROS level is frequently observed in the course of morphine addiction, and ROS is proverbially involved in this process. This study aims to explore the relationship among morphine addiction, reactive oxygen species (ROS) and expression of μ-opioid receptor (MOR) in differentiated SH-SY5Y cells. Main methods SH-SY5Y cells were induced to differentiation by treatment with retinoic acid (RA); the activity of lactate dehydrogenase (LDH) and the nitro blue tetrazolium (NBT) reduction were assessed by spectrophotometry. Intracellular reactive oxygen species (ROS) was measured with the 2,7-dichlorofluorescin diacetate (DCFH-DA) assay. Cellular cAMP was determined by using a competitive protein binding kit. The mRNA expression of μ-opioid receptor (MOR) was evaluated by qRT-PCR. Key findings Morphine-induced ROS are generated in a concentration- and time-dependent manner and inhibited by naloxone. Exogenous oxidants increase the level of ROS and aggravate morphine addiction, while the exogenous antioxidants efficiently reverse these effects. Morphine decreases the mRNA level of MOR in a concentration-dependent manner. And the mRNA level of MOR is remarkably reduced in the presence of exogenous oxidants and effectively promoted by antioxidants. Significance This study indicates that ROS can affect morphine addiction through involving MOR. Treatment with ROS scavenging can serve as a medical therapy for morphine addiction. © 2015 Elsevier Inc. All rights reserved. Source

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