Liu N.,Linyi Central Hospital |
Sun S.,Linyi Central Hospital |
Yang X.,Qianfoshan Hospital
International Journal of Clinical and Experimental Pathology | Year: 2017
Background: SET and MYND domain-containing protein 3 (SMYD3) is a histone methyltransferases and it promotes progression of many kinds of cancers including lung cancer, ovarian cancer and gastric cancer. In colorectal cancer (CRC), SMYD3 is proved to stimulate the proliferation of cancer cells, but the clinical significance of SMYD3 in CRC has not been elucidated. Methods: In our study, we detected the expression of SMYD3 in CRC samples in TNM stage I-III with immunohistochemistry. The correlation between the expression of SMYD3 and the clinicopathological factors was analyzed with Chi-square test. The survival curve was displayed by Kaplan-Meier test and the statistical difference of subgroups was analyzed with log-rank test. Independent prognostic factors were identified by the Cox proportional hazards regression model. Results: The percentage of high SMYD3 expression and low expression accounts for 47.98% and 52.02% respectively. High expression of SMYD3 was significantly associated with advance T stage (P=0.006) and lower survival rates (P=0.010), and it could be identified as an independent prognostic factor indicating unfavorable prognosis of patients with CRC (P=0.032, HR=1.98, 95% CI=1.06-3.70). Conclusions: SMYD3 high-expression is a high risk for poorer prognosis of CRC in TNM stage I-III. Our findings suggested that detecting SMYD3 may help stratify patients by risk more preciously and help make the individual treatment strategy.
Zhai L.,Shandong University |
Zhai L.,Qingdao University |
Zhang P.,Shandong University |
Sun R.-Y.,Shandong University |
And 3 more authors.
Pharmacological Reports | Year: 2011
A novel stilbene derivative, (E)-2-(2-chlorostyryl)-3,5,6-trimethylpyrazine (CSTMP), was designed and synthesized based on the pharmacophores of tetramethylpyrazine (TMP) and resveratrol (RES). In the present study, we investigated the protective effects of CSTMP on vascular endothelial cells under oxidative stress and elucidated its molecular mechanisms. The radical scavenging activity of CSTMP was assessed by the DPPH test. Human Umbilical Vein Endothelial Cells (HUVECs) were exposed to 150 μM hydrogen peroxide (H 2O 2) for 12 h, resulting in a decrease of cell viability assessed by the MTT assay and an increase of apoptotic cells assessed by the nuclear staining assay and flow cytometry. The activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD) and nitric oxide synthase (NOS) and the contents of malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide (NO) in cells were determined by commercial kits. The expression levels of pro-apoptotic factor caspase-3 and anti-apoptotic signal ERK1/2 were detected by western blot. The results showed that CSTMP had a moderate anti-oxidative effect against the DPPH test, which was less than RES. Co-incubation with CSTMP increased the cell viability, markedly reduced the LDH leakage from the cells and decreased the lipid peroxidation. These effects of CSTMP were accompanied by increasing activity of the endogenous antioxidant enzyme SOD, the level of GSH, the production of NO and cNOS activity. Moreover, CSTMP showed stronger effects on the inhibition of apoptosis, caspase-3 expression, and the activation of phosphorylated ERK1/2 compared to RES. Furthermore, CSTMP could inhibit the expression of phospho-JNK and phospho-p38 induced by H 2O 2. These results suggest that CSTMP prevents H 2O 2-induced cell injury through anti-oxidation and anti-apoptosis via the MAPK and caspase-3 pathways. Copyright © 2011 by Institute of Pharmacology Polish Academy of Sciences.
Cao B.,Capital Medical University |
Zhu L.,Peking University |
Zhu S.,Capital Medical University |
Li D.,Qianfoshan Hospital |
And 3 more authors.
Cancer Immunology, Immunotherapy | Year: 2010
Purpose: T cell immunoglobulin and mucin domain-containing molecule 3 (TIM-3) could weaken the Th1-mediated anti-tumor responses and accelerate the tumor cell proliferation by inhabiting the production of IL-2 or IFN-γ. This study was to assess the association between TIM-3 genetic variations and the development of gastric cancer. Patients and methods: Five polymorphisms located in the promoter or encoding region of TIM-3 gene were genotyped in 212 gastric cancer patients and 252 controls who matched with the patients on the frequency of age, gender, smoking, and drinking. Logistic regression was used to determine whether the inherited variations within TIM-3 gene were associated with gastric cancer risk. Linkage disequilibrium and Haplotype analyses were performed by using SHEsis program. Results: By the individual genotype analysis, three polymorphisms (-574G/T, -882C/T, and -1516G/T) within TIM-3 gene were significantly associated with gastric cancer in the study population [ORs (95% CIs): 2.74 (1.21-6.20), 3.19 (1.29-7.91), and 2.03 (1.15-3.59); respectively]. Among the gastric cancer patients, the relationship between the -1516 polymorphic genotype and the distant metastasis of tumor was found (OR = 2.21, 95% CI = 1.05-4.63). Under the analysis of haplotypes, an even stronger association with haplotype TTGCT was observed in gastric cancer risk (OR = 5.57, 95% CI: 1.04-29.80, P = 0.024). Conclusion: These results indicated that the three genetic variations within the TIM-3 gene promoter may be associated with the increased susceptibility to gastric cancer, especially among the haplotypes with the risk. © 2010 Springer-Verlag.
Zhu L.-M.,Yantai University |
Lin P.,Yantai University |
Dai T.,Yantai University |
Zhang C.-H.,Yantai University |
Ma R.-P.,Qianfoshan Hospital
Latin American Journal of Pharmacy | Year: 2017
Cardiovascular diseases remains to threaten the health of humans, and Danshen is a traditional Chinese herbal medicine widely used to treat cardiovascular diseases. Clinical herb-drug interaction during the therapy of cardiovascular diseases was evaluated in the present study. Two major ingredients salvianolic acid A and C from Danshen were docked into the activity cavity of UDP-glucuronosyltransferase (UGT) 1A8 which has been accepted to be an important UGT isoform, trying to indicate UGT1A8 inhibition-based herb-drug interaction. Homology modeling method was used to construct the crystal structure of UGT1A8, and Autodock Version 4.2 was used to dock the salvianolic acid A/C into the activity cavity of UGT1A8. The active pocket binding with salvianolic acid A/C consisted of the following amino acids residues: Gly35, Ser36, His37, Phe39, Leu83, Phe93, Asp95, Trp98, Leu105, Phe106, Ser107, Leu108, Phe109, Ser111, Ser112, Asp115, Phe116, Arg170, Ser303, Ser306, Met307, Arg333, Gln354, Phe366, His369, Ala370, Gly371, Ser372, His373, Gly374, Phe391, Asp393, and Gln394. The hydrogen bonds were formed between salvianolic acid A/C and some amino acids residues located in the activity cavity of UGT1A8. Salvianolic acid A formed five hydrogen bonds with four amino acids residues in the binding pocket of UGT1A8, including N atom of Ser36, NH1 atom of Arg170, OD1 and OD2 atoms of Asp393, and ND1 atom of His373. Salvianolic acid C formed seven hydrogen bonds to five residues, including OD1 atom of Asp95, OG atom of Ser112, OG atom of Ser306, NH1 atom of Arg333, and OE1 atom of Gln354. Besides the hydrogen bonds, Salvianolic acid A and C also formed hydrophobic interactions with the activity cavity of UGT1A8. Salvianolic acid A formed hydrophobic contacts with non-charged polar residues Ser36, Ser112, Ser306, Gly371, charged residues His37, His369, His373, Asp393, and non-polar residues Trp98, Leu105, Phe109, Phe116, Met307, Phe391. Salvianolic acid C formed hydrophobic contacts to non-charged polar residues Ser112, Gly115, Ser306, Gln354, Gly371, charged residues His369, His373, non-polar residues Phe39, Leu83, Trp98, Leu105, Phe116, Met307, Phe391. In conclusion, the present study demonstrated the inhibition of salvianolic acid A/C on the activity of UGT1A8 using in silico docking method. © 2017, Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
Luan X.,Binzhou Medical University |
Li G.,Qianfoshan Hospital |
Wang G.,Binzhou Medical University |
Wang F.,Binzhou Medical University |
Lin Y.,Red Cross
Tissue and Cell | Year: 2013
Human placenta-derived mesenchymal stem cells (hPMSCs) have been shown to possess immunosuppressive effects against T cells and support the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB). However, the characteristics of hPMSCs compared with human bone marrow-derived mesenchymal stem cells (hBMSCs) are not fully understood. Here, we show that hPMSCs have similar regulatory effects on T cell activation, proliferation and cytokine secretion as hBMSCs and demonstrate that PDL1 and B7H4, negative co-stimulatory molecules, are involved in the T cell immunosuppressive activities of hPMSCs and hBMSCs, respectively. hPMSCs efficiently enhanced the expansion of CD34+ cells from UCB compared with hBMSCs. Furthermore, hPMSCs maintained the expression of adhesion molecules (CD11a, CD44 and CD49e) in CD34+ cells. Similar effects were observed for both hPMSCs and hBMSCs on CD34+ cell chemotaxis and cytokine production, such as SDF-1α, IL-6 and SCF. Therefore, hPMSCs may be an ideal alternative source of hBMSCs for basic research and clinical applications, which may be significant in future efforts to explore the potential clinical utility of hPMSCs. © 2012 Elsevier Ltd.
Ling J.,Michigan State University |
Anderson L.M.,State University of New York at Buffalo |
Ji H.,Qianfoshan Hospital
School Psychology International | Year: 2015
This article reviews the results of a school-based self-management intervention for Chinese obese children at risk for metabolic syndrome. Twenty-eight Chinese obese children (M age = 10 years) and their parents participated in the study. Metabolic syndrome risk factors were measured pre- and post-intervention. The risk factors included Body Mass Index, waist circumstance, systolic blood pressure, diastolic blood pressure, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, High-sensitivity C-reactive Protein, fasting plasma glucose, and fasting blood insulin. After 6 months of school-based self-management intervention, all risk factors with the exception of triglycerides and fasting plasma glucose changed significantly in the healthful direction (p < 0.01). This pilot study offers promising implications for school interventions that are delivered by school-based practitioners collaborating with parents, teachers, and children. An individualized program based on a self-care and -management framework may have utility as a relatively cost-effective, school-based intervention to improve children’s health. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav
Dun Z.,Shandong University |
Zhu S.,Shandong University |
Jiang H.,Qianfoshan Hospital
Journal of International Medical Research | Year: 2013
ObjectiveFrame-based stereotactic surgical planning systems (SSPSs) have been used for deep brain stimulation and radioneurosurgery. Here, we evaluated the feasibility, safety and efficacy of using a SSPS to aid spontaneous intracerebral haematoma (ICH) treatment.MethodsPatients with moderate spontaneous putamen haematomas were randomized into two groups: treatment (group A) and control (group B). In group B, the catheter for evacuating haematomas was inserted into a target point, located at the centre of the haematoma, using conventional frame-based stereotactics; urokinase thrombolysis was subsequently delivered through the catheter. In group A, this procedure was assisted by a SSPS, which designed both the target point and trajectory in the haematoma through virtual reality. Duration of evacuating haematomas and number of urokinase injections was compared between groups.ResultsIn total, 65 patients were recruited: in group A (n = 30), the duration of evacuating haematomas (35.27 ± 9.17 h) was shorter than in group B (n = 35; 67.77 ± 13.82 h). There were fewer urokinase injections in group A (3.63 ± 1.16) than in group B (6.40 ± 1.29).ConclusionsThe feasibility, efficacy and safety of spontaneous ICH treatment were optimized by the use of a frame-based SSPS. © The Author(s) 2013.
Qu Y.,Shandong University |
Yang Y.,Qianfoshan Hospital |
Ma D.,Shandong University |
Xiao W.,Shandong University
Oncology Reports | Year: 2012
Lung cancer is the most common cause of cancer-related deaths in the world. The trefoil factor (TFF) family is composed of three thermostable, and protease-resistant proteins, named TFF1, TFF2 and TFF3. TFF protein levels have been found to be related to the development of various types of cancer. However, it is still unclear whether TFF proteins are differentially expressed in the serum of different histological subtypes of lung cancer compared to healthy individuals. In this study, we investigated the levels of TFF proteins in serum and lung tissues of 130 lung cancer patients (58 squamous cell lung carcinoma cases, 43 adenocarcinoma cases and 29 SCLC cases) and 60 healthy individuals. It was found that TFF1 and TFF2 have similar or slightly higher levels in these three subtypes of lung cancer compared to healthy individuals, while TFF3 levels were significantly higher in the examined lung cancer cases compared to healthy individuals. Immunoblot analyses of TFF1, TFF2 and TFF3 indicated that lung cancer tissues and lung cancer cell lines have a higher expression of the TFF3 protein, but not of TFF1 or TFF2 proteins, compared to tissues from healthy individuals or from the normal cell line. Quantitative RT-PCR analysis indicated higher levels of TFF3, but not TFF1 and TFF2, transcripts in lung cancer tissues or cell lines. These results show increased TFF3 levels in serum and lung tissues, suggesting that TFF3 may serve as a promising, easily detected biomarker of lung cancer.
Li Y.,Shandong University |
Cao F.,Shandong University |
Cao D.,Qianfoshan Hospital |
Wang Q.,Shandong University |
Cui N.,Shandong University
Journal of Pediatric Surgery | Year: 2012
Background/Purpose: Posttraumatic growth (PTG) is a positive psychological change, or benefit, as a result of a major life trauma and/or loss. The role of emotional intelligence (EI), social support, self-efficacy, posttraumatic stress symptoms (PTSS), and resilience in PTG was evaluated in parents of children undergoing a surgical procedure for congenital disease. Methods: A questionnaire survey was conducted in 208 parents of children undergoing inpatient surgery for correction of congenital disease at the Departments of Pediatric Surgery, Qilu and Shandong Province Hospital, China, between May and September. Posttraumatic growth was measured using the Posttraumatic Growth Inventory. The study variables were measured using the Emotional Intelligence Scale, Perceived Social Support Scale, General Self-Efficacy Scale, Posttraumatic Stress Disorder Checklist-Civilian Version, and the 10-item Connor-Davidson Resilience Scale, respectively. Hierarchical multiple regression analyses were used to identify the significant predictors. Results: Posttraumatic growth was reported in 54.3% (113) of parents. Posttraumatic growth was positively associated with EI, self-efficacy, resilience, and PTSS (total P <.05). Social support was positively associated with the domain of relating to others in the Posttraumatic Growth Inventory (r =.208, P <.01). Hierarchical multiple regression analyses indicated EI was the main predictor of posttraumatic growth, whereas resilience and PTSS were the mediators of personal resources (EI and self-efficacy), perceived social support, and the growth. Conclusions: The relationship between PTG and other variables have been examined at only one point in time. Longitudinal studies in exploring the impact of EI and other variables affecting PTG can be used to reduce the impact of bidirectionality that may have impact on the conclusions drawn. Emotional management programs should focus on the use of EI as a proactive measure for enhancing emotional well-being and promoting positive emotions. © 2012 Elsevier Inc.
Xu W.-J.,Qianfoshan Hospital |
Guo B.-L.,Zhangqiu Maternity and Child Care hospital |
Han Y.-G.,Electrical Hospital |
Shi L.,Jining Medical University |
Ma W.-S.,Qianfoshan Hospital
Tumor Biology | Year: 2014
This study aimed to investigate the clinical values of serum alpha-fetoprotein-L3 (AFP-L3) and Golgi protein 73 (GP73) in the diagnosis of hepatocellular carcinoma (HCC) with low alpha-fetoprotein (AFP). From January 2011 to December 2013, 50 low-AFP HCC patients confirmed by the color Doppler flow imaging (CDFI) and pathological examinations were collected. Forty-five patients with chronic liver diseases were also selected, including 29 liver cirrhosis patients, 15 chronic hepatitis B patients, and one severe hepatitis patient. Furthermore, 100 health volunteers with no evidence of benign or malignant liver diseases were included. The enzyme-linked immunosorbent assay (ELISA) method was applied to the GP73 quantitative assay. Serum AFP concentrations were determined using immunoassays utilizing enhanced chemiluminescence. Diagnostic accuracy of GP73 and AFP-L3 assays for low-AFP HCC was evaluated using receiver operating characteristic (ROC) curve analysis. Statistical analyses were conducted with the GraphPad Prism 5.0 software. Low-AFP HCC patients (35/50) exhibited higher positive rates of AFP-L3 than non-HCC patients (5/45) and healthy controls (2/100) (both P < 0.05). There were also significant differences in the positive rate of GP73 of low-AFP HCC patients (40/50) compared to those of non-HCC patients (3/45) and healthy controls (1/100) (both P < 0.05). However, no obvious differences in the positive rates of AFP-L3 and GP73 were observed between non-HCC patients and healthy controls (both P > 0.05). ROC curves showed that the area under the curve (AUC) of AFP-L3 for the diagnosis of low-AFP HCC was 0.6994 (sensitivity [Sen] = 70.0 %, specificity [Spe] = 95.2 %, accuracy = 88.7 %), while the AUC of GP73 was 0.8411 (Sen = 80.0 %, Spe = 97.2 %, accuracy = 92.8 %). Compared with single detection, the combination of AFP-L3 and GP73 levels for the diagnosis of low-AFP HCC showed higher Sen (94.0 %), Spe (93.1 %), and better accuracy (93.3 %). Our findings provide empirical evidence that the combination of AFP-L3 and GP73 is a good diagnostic strategy for low-AFP HCC. © 2014, International Society of Oncology and BioMarkers (ISOBM).