Jinan, China
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Cao B.,Capital Medical University | Zhu L.,Peking University | Zhu S.,Capital Medical University | Li D.,Qianfoshan Hospital | And 3 more authors.
Cancer Immunology, Immunotherapy | Year: 2010

Purpose: T cell immunoglobulin and mucin domain-containing molecule 3 (TIM-3) could weaken the Th1-mediated anti-tumor responses and accelerate the tumor cell proliferation by inhabiting the production of IL-2 or IFN-γ. This study was to assess the association between TIM-3 genetic variations and the development of gastric cancer. Patients and methods: Five polymorphisms located in the promoter or encoding region of TIM-3 gene were genotyped in 212 gastric cancer patients and 252 controls who matched with the patients on the frequency of age, gender, smoking, and drinking. Logistic regression was used to determine whether the inherited variations within TIM-3 gene were associated with gastric cancer risk. Linkage disequilibrium and Haplotype analyses were performed by using SHEsis program. Results: By the individual genotype analysis, three polymorphisms (-574G/T, -882C/T, and -1516G/T) within TIM-3 gene were significantly associated with gastric cancer in the study population [ORs (95% CIs): 2.74 (1.21-6.20), 3.19 (1.29-7.91), and 2.03 (1.15-3.59); respectively]. Among the gastric cancer patients, the relationship between the -1516 polymorphic genotype and the distant metastasis of tumor was found (OR = 2.21, 95% CI = 1.05-4.63). Under the analysis of haplotypes, an even stronger association with haplotype TTGCT was observed in gastric cancer risk (OR = 5.57, 95% CI: 1.04-29.80, P = 0.024). Conclusion: These results indicated that the three genetic variations within the TIM-3 gene promoter may be associated with the increased susceptibility to gastric cancer, especially among the haplotypes with the risk. © 2010 Springer-Verlag.


Luan X.,Binzhou Medical University | Li G.,Qianfoshan Hospital | Wang G.,Binzhou Medical University | Wang F.,Binzhou Medical University | Lin Y.,Red Cross
Tissue and Cell | Year: 2013

Human placenta-derived mesenchymal stem cells (hPMSCs) have been shown to possess immunosuppressive effects against T cells and support the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB). However, the characteristics of hPMSCs compared with human bone marrow-derived mesenchymal stem cells (hBMSCs) are not fully understood. Here, we show that hPMSCs have similar regulatory effects on T cell activation, proliferation and cytokine secretion as hBMSCs and demonstrate that PDL1 and B7H4, negative co-stimulatory molecules, are involved in the T cell immunosuppressive activities of hPMSCs and hBMSCs, respectively. hPMSCs efficiently enhanced the expansion of CD34+ cells from UCB compared with hBMSCs. Furthermore, hPMSCs maintained the expression of adhesion molecules (CD11a, CD44 and CD49e) in CD34+ cells. Similar effects were observed for both hPMSCs and hBMSCs on CD34+ cell chemotaxis and cytokine production, such as SDF-1α, IL-6 and SCF. Therefore, hPMSCs may be an ideal alternative source of hBMSCs for basic research and clinical applications, which may be significant in future efforts to explore the potential clinical utility of hPMSCs. © 2012 Elsevier Ltd.


Ling J.,Michigan State University | Anderson L.M.,State University of New York at Buffalo | Ji H.,Qianfoshan Hospital
School Psychology International | Year: 2015

This article reviews the results of a school-based self-management intervention for Chinese obese children at risk for metabolic syndrome. Twenty-eight Chinese obese children (M age = 10 years) and their parents participated in the study. Metabolic syndrome risk factors were measured pre- and post-intervention. The risk factors included Body Mass Index, waist circumstance, systolic blood pressure, diastolic blood pressure, triglycerides, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, High-sensitivity C-reactive Protein, fasting plasma glucose, and fasting blood insulin. After 6 months of school-based self-management intervention, all risk factors with the exception of triglycerides and fasting plasma glucose changed significantly in the healthful direction (p < 0.01). This pilot study offers promising implications for school interventions that are delivered by school-based practitioners collaborating with parents, teachers, and children. An individualized program based on a self-care and -management framework may have utility as a relatively cost-effective, school-based intervention to improve children’s health. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav


Liang Z.,Shandong University | Liang Z.,Yantaishan Hospital | Sun X.-Y.,Reproductive Medical Center | Xu L.-C.,Yantaishan Hospital | Fu R.-Z.,Qianfoshan Hospital
Medical Science Monitor | Year: 2014

Background: Increased amounts of soluble E-cadherin (E-cad) have been found in the serum in various cancers, but the role of serum soluble E-cad in the prognosis of breast cancer patients has not been explored in Asian populations. Material/Method: Blood samples from 111 consecutive patients diagnosed with breast cancer and 55 healthy controls were in-vestigated. Serum soluble E-cad expression levels were measured by enzyme-linked immunosorbent assay (ELISA) with an immunoassay kit according to the manufacturer’s directions. Kaplan-Meier analyses were used to evaluate the association between serum soluble E-cad expression level and survival. All statistical tests were 2-sided. Results: The serum levels of soluble E-cad in breast cancer patients were significantly higher than those of the control group (2218.9±319.6 ng/ml vs. 742.8±91.7 ng/ml, p<0.001). Serum levels of soluble E-cad correlated signifi- cantly with TNM stage (P=0.007), tumor grade (P=0.03), and lymph node metastasis (P<0.001). Kaplan-Meier analysis with the log-rank test indicated that high serum levels of soluble E-cad had a significant impact on overall survival (55.4% vs. 81.4%; P=0.032) and disease-free survival (36.8% vs. 67.8%; P=0.002) in breast can- cer. Multivariate analysis revealed that serum levels of soluble E-cad were independently associated with over-all survival and disease-free survival in breast cancer patients. Conclusions: Serum soluble E-cad level is an independent prognostic factor in Asian breast cancer patients. © Med Sci Monit, 2014; 20: 2776-2782.


Qu Y.,Shandong University | Yang Y.,Qianfoshan Hospital | Ma D.,Shandong University | He L.,Shandong University | Xiao W.,Shandong University
Molecular Biology Reports | Year: 2013

Chronic obstructive pulmonary disease (COPD) is associated with chronic severe airway inflammation and causes increasing global health problems. New biological markers for COPD prediction and prognosis are urgently necessary. Previous studies indicate that histone deacetylases (HDACs) play essential roles in COPD. This study is to investigate if HDAC2 levels can be used as a promising, easily detected biomarker of COPD. In this paper, 49 COPD patients were enrolled and 42 healthy individuals (smokers or non-smokers) were used as healthy controls. Human bronchial epithelial cells derived from non-smokers, smokers, or COPD patients were grown in primary cultures. Total proteins were harvested from lung tissues or bronchial epithelial cells and then subjected to immunoblot analyses of HDAC2, HDAC3, and HDAC5. Quantitative RT-PCR analysis of HDAC2, HDAC3, and HDAC5 mRNA levels in tissues or cells were also preformed. We found that among the three HDAC proteins, the mRNA and protein levels of HDAC2, but not HDAC3 and HDAC5, in the tissues or cultured cells from patients have a significant correlation with development and prognosis of COPD. These results suggested that HDAC2 levels may serve as a promising, easily detected biomarker of COPD. © 2012 Springer Science+Business Media Dordrecht.


Dun Z.,Shandong University | Zhu S.,Shandong University | Jiang H.,Qianfoshan Hospital
Journal of International Medical Research | Year: 2013

ObjectiveFrame-based stereotactic surgical planning systems (SSPSs) have been used for deep brain stimulation and radioneurosurgery. Here, we evaluated the feasibility, safety and efficacy of using a SSPS to aid spontaneous intracerebral haematoma (ICH) treatment.MethodsPatients with moderate spontaneous putamen haematomas were randomized into two groups: treatment (group A) and control (group B). In group B, the catheter for evacuating haematomas was inserted into a target point, located at the centre of the haematoma, using conventional frame-based stereotactics; urokinase thrombolysis was subsequently delivered through the catheter. In group A, this procedure was assisted by a SSPS, which designed both the target point and trajectory in the haematoma through virtual reality. Duration of evacuating haematomas and number of urokinase injections was compared between groups.ResultsIn total, 65 patients were recruited: in group A (n = 30), the duration of evacuating haematomas (35.27 ± 9.17 h) was shorter than in group B (n = 35; 67.77 ± 13.82 h). There were fewer urokinase injections in group A (3.63 ± 1.16) than in group B (6.40 ± 1.29).ConclusionsThe feasibility, efficacy and safety of spontaneous ICH treatment were optimized by the use of a frame-based SSPS. © The Author(s) 2013.


Qu Y.,Shandong University | Yang Y.,Qianfoshan Hospital | Ma D.,Shandong University | Xiao W.,Shandong University
Oncology Reports | Year: 2012

Lung cancer is the most common cause of cancer-related deaths in the world. The trefoil factor (TFF) family is composed of three thermostable, and protease-resistant proteins, named TFF1, TFF2 and TFF3. TFF protein levels have been found to be related to the development of various types of cancer. However, it is still unclear whether TFF proteins are differentially expressed in the serum of different histological subtypes of lung cancer compared to healthy individuals. In this study, we investigated the levels of TFF proteins in serum and lung tissues of 130 lung cancer patients (58 squamous cell lung carcinoma cases, 43 adenocarcinoma cases and 29 SCLC cases) and 60 healthy individuals. It was found that TFF1 and TFF2 have similar or slightly higher levels in these three subtypes of lung cancer compared to healthy individuals, while TFF3 levels were significantly higher in the examined lung cancer cases compared to healthy individuals. Immunoblot analyses of TFF1, TFF2 and TFF3 indicated that lung cancer tissues and lung cancer cell lines have a higher expression of the TFF3 protein, but not of TFF1 or TFF2 proteins, compared to tissues from healthy individuals or from the normal cell line. Quantitative RT-PCR analysis indicated higher levels of TFF3, but not TFF1 and TFF2, transcripts in lung cancer tissues or cell lines. These results show increased TFF3 levels in serum and lung tissues, suggesting that TFF3 may serve as a promising, easily detected biomarker of lung cancer.


Liu J.,Qianfoshan Hospital | Wang A.,Qianfoshan Hospital | Li L.,Shandong University | Huang Y.,Qianfoshan Hospital | And 2 more authors.
Seizure | Year: 2010

Oxidative stress, which is defined as the over-production of free radicals, can dramatically alter neuronal function and has been linked to status epilepticus (SE). The pathological process and underlying mechanisms involved in the oxidative stress during SE are still not fully clear. In the current study, SE was induced in rats by lithium-pilocarpine administration. Our data show that hippocampal neuron death occurs at 6 h and is sustained for 7 days after SE. The production of nitric oxide (NO) started to increase at 30 min and was evident at 6 h and 7 days after SE, which coincided with increased expression of neuronal nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS) and malondialdehyde (MDA) after SE, whereas, activated caspase-3 prominently appeared at 7 days after SE. Further, FK506, an immunosuppressant, partially rescued the neuron death and attenuated the expression of NO, nNOS, iNOS, MDA and activated caspase-3. Taken together, our study indicates that oxidative stress mediated hippocampal neuron death occurs prior to caspase-3 activation and that FK506 plays an important role in protecting hippocampal neurons during status epilepticus. © 2010 British Epilepsy Association.


Li Y.,Shandong University | Cao F.,Shandong University | Cao D.,Qianfoshan Hospital | Wang Q.,Shandong University | Cui N.,Shandong University
Journal of Pediatric Surgery | Year: 2012

Background/Purpose: Posttraumatic growth (PTG) is a positive psychological change, or benefit, as a result of a major life trauma and/or loss. The role of emotional intelligence (EI), social support, self-efficacy, posttraumatic stress symptoms (PTSS), and resilience in PTG was evaluated in parents of children undergoing a surgical procedure for congenital disease. Methods: A questionnaire survey was conducted in 208 parents of children undergoing inpatient surgery for correction of congenital disease at the Departments of Pediatric Surgery, Qilu and Shandong Province Hospital, China, between May and September. Posttraumatic growth was measured using the Posttraumatic Growth Inventory. The study variables were measured using the Emotional Intelligence Scale, Perceived Social Support Scale, General Self-Efficacy Scale, Posttraumatic Stress Disorder Checklist-Civilian Version, and the 10-item Connor-Davidson Resilience Scale, respectively. Hierarchical multiple regression analyses were used to identify the significant predictors. Results: Posttraumatic growth was reported in 54.3% (113) of parents. Posttraumatic growth was positively associated with EI, self-efficacy, resilience, and PTSS (total P <.05). Social support was positively associated with the domain of relating to others in the Posttraumatic Growth Inventory (r =.208, P <.01). Hierarchical multiple regression analyses indicated EI was the main predictor of posttraumatic growth, whereas resilience and PTSS were the mediators of personal resources (EI and self-efficacy), perceived social support, and the growth. Conclusions: The relationship between PTG and other variables have been examined at only one point in time. Longitudinal studies in exploring the impact of EI and other variables affecting PTG can be used to reduce the impact of bidirectionality that may have impact on the conclusions drawn. Emotional management programs should focus on the use of EI as a proactive measure for enhancing emotional well-being and promoting positive emotions. © 2012 Elsevier Inc.


Xu W.-J.,Qianfoshan Hospital | Guo B.-L.,Zhangqiu Maternity and Child Care hospital | Han Y.-G.,Electrical Hospital | Shi L.,Jining Medical University | Ma W.-S.,Qianfoshan Hospital
Tumor Biology | Year: 2014

This study aimed to investigate the clinical values of serum alpha-fetoprotein-L3 (AFP-L3) and Golgi protein 73 (GP73) in the diagnosis of hepatocellular carcinoma (HCC) with low alpha-fetoprotein (AFP). From January 2011 to December 2013, 50 low-AFP HCC patients confirmed by the color Doppler flow imaging (CDFI) and pathological examinations were collected. Forty-five patients with chronic liver diseases were also selected, including 29 liver cirrhosis patients, 15 chronic hepatitis B patients, and one severe hepatitis patient. Furthermore, 100 health volunteers with no evidence of benign or malignant liver diseases were included. The enzyme-linked immunosorbent assay (ELISA) method was applied to the GP73 quantitative assay. Serum AFP concentrations were determined using immunoassays utilizing enhanced chemiluminescence. Diagnostic accuracy of GP73 and AFP-L3 assays for low-AFP HCC was evaluated using receiver operating characteristic (ROC) curve analysis. Statistical analyses were conducted with the GraphPad Prism 5.0 software. Low-AFP HCC patients (35/50) exhibited higher positive rates of AFP-L3 than non-HCC patients (5/45) and healthy controls (2/100) (both P < 0.05). There were also significant differences in the positive rate of GP73 of low-AFP HCC patients (40/50) compared to those of non-HCC patients (3/45) and healthy controls (1/100) (both P < 0.05). However, no obvious differences in the positive rates of AFP-L3 and GP73 were observed between non-HCC patients and healthy controls (both P > 0.05). ROC curves showed that the area under the curve (AUC) of AFP-L3 for the diagnosis of low-AFP HCC was 0.6994 (sensitivity [Sen] = 70.0 %, specificity [Spe] = 95.2 %, accuracy = 88.7 %), while the AUC of GP73 was 0.8411 (Sen = 80.0 %, Spe = 97.2 %, accuracy = 92.8 %). Compared with single detection, the combination of AFP-L3 and GP73 levels for the diagnosis of low-AFP HCC showed higher Sen (94.0 %), Spe (93.1 %), and better accuracy (93.3 %). Our findings provide empirical evidence that the combination of AFP-L3 and GP73 is a good diagnostic strategy for low-AFP HCC. © 2014, International Society of Oncology and BioMarkers (ISOBM).

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