Deng S.,Shanghai University |
Deng S.,Xinjiang Medical University |
Zhou Z.,Shanghai University |
Zhou Z.,Puyang Oilfield General Hospital |
And 16 more authors.
British Journal of Dermatology | Year: 2015
Background Tinea capitis is very common in Western China, with the most widespread aetiological agent being Trichophyton violaceum, while Microsporum canis is prevalent in the remainder of China. Conventional diagnostics and internal transcribed spacer (ITS) sequencing analyses have proven relatively limited due to the close phylogenetic relationship of anthropophilic dermatophytes. Therefore, alternative molecular tools with sufficient specificity, reproducibility and sensitivity are necessary. Objectives To evaluate two molecular techniques [multiplex ligation-dependent probe amplification (MLPA) and rolling circle amplification (RCA)] for rapid detection of the aetiological agents of tinea capitis, T. violaceum and M. canis. Methods Probes of RCA and MLPA were designed with target sequences in the rDNA ITS gene region. Strains tested consist of 31 T. violaceum, 22 M. canis and 24 reference strains of species that are taxonomically close to the target species. Results The specificity and reproducibility of RCA and MLPA in detection of T. violaceum and M. canis were both 100% in both species. Sensitivity testing showed that RCA was positive at concentrations down to 1·68 × 106 copies of DNA in the TvioRCA probe, and 2·7 × 108 copies of DNA in McRCA. MLPA yielded positive results at concentrations of DNA down to 1·68 × 101 copies in the TvioMLPA probe and 2·7 × 102 in McMLPA. Conclusions The two techniques were sufficiently specific and sensitive for discriminating the target DNA of T. violaceum and M. canis from that of closely related dermatophytes. RCA and MLPA are advantageous in their reliability and ease of operation compared with standard polymerase chain reaction and conventional methods. © 2015 British Association of Dermatologists.
Jiang T.,Capital Medical University |
Tang G.-F.,Anhui Medical University |
Lin Y.,Liaoning Medical University |
Peng X.-X.,Capital Medical University |
And 9 more authors.
Chinese Medical Journal | Year: 2011
Background Although the first leading cause of death in China was malignant neoplasms (mortality, 374.1 per 100 000 person-years), the full impact of primary brain tumors (PBT) on the healthcare system is not completely described because there are a few well documented reports about the epidemiologic features of brain tumors. This study aimed to report a comprehensive assessment on the prevalence of PBT. Methods A multicenter cross-sectional study on brain tumor (MCSBT) in China was initiated in five regional centers: Daqing (northeast), Puyang (north of China), Shiyan (center of China), Ma'anshan (center of China) and Shanghai (southeast). Prevalence rate was calculated by counting the number of people living with a PBT between October 1, 2005 and September 30, 2006 and dividing by the total population of the five communities at January 1, 2006. Estimates of prevalence were expressed as percentages and grouped according to gender and to age in fifteen-year categories. Within these strata, the rates were estimated with 95% confidence intervals (CI) using the accurate calculation of CI for Poisson distribution. A chi-square test was used to compare the various frequencies with α <0.05. Age-standardized prevalence with the direct method was calculated with the ten-year age-specific prevalence and the age distribution of population prospects: the 2008 revision.Results We estimated that the overall prevalence of PBT was 24.56 per 100 000 (95% CI, 14.85 to 34.27), and the overall prevalence of PBT in female population (30.57 per 100 000 and its 95% CI ranged from 19.73 to 41.41) was higher than that in male population (18.84 per 100 000 and its 95% CI ranged from 10.33 to 27.35). However, the discrepancy between genders was not statistically significant because the 95% CI overlapped. Of 272 cases of newly diagnosed PBT, the proportion of histological subtypes by age groups, gender was statistically different (χ 2=52.6510, P <0.0001). More than half of all reported tumors (52.57%) were either gliomas or meningiomas. For the youngest (aged from 0-19) strata of the population, glioma appeared to occur more than other subtypes, accounting for 55.56% of all of cases. The majority of brain tumors presented in those aged from 20 to 59 years was pituitary adenomas (45.12%) and gliomas (31.10%). Opposed to brain tumors in adults and teenage, gliomas only accounted for 22.22%. Meanwhile, the median ages at diagnosis of the patients with PBT were similar between males and females except for pituitary adenomas (male: 59 years old; female: 45 years old). Conclusions Age standardized prevalence of PBT is 22.52 per 100 000 (95% CI, 13.22 to 31.82) for all populations, 17.64 per 100 000 (95% CI, 9.41 to 25.87) for men, and 27.94 per 100 000 (95% CI, 17.58 to 38.30) for women. Age standardization to China's 2010 population yielded an estimated population of 304 954 cases with PBT. Our prevalence estimates provide a conservative basis on which to plan health care services and to develop programmatic strategies for surviving. In the future, it would be helpful to have long-term observed survival rates that would make the assumptions and the resulting imprecision in the current estimates unnecessary.
Yan Y.,Southern Medical University |
Sha Y.-H.,Southern Medical University |
Li X.-Z.,Puyang Oilfield General Hospital |
Wang S.-G.,Southern Medical University |
And 3 more authors.
Chinese Journal of Evidence-Based Medicine | Year: 2015
To systematically review the efficacy and safety of laparoscopic Roux-en-Y gastric bypass (LRYGB) versus laparoscopic sleeve gastrectomy (LSG) for obesity and type 2 diabetes mellitus (T2DM). Methods We searched PubMed, EMbase, The Cochrane Library (Issue 8, 2014), CNKI and WanFang Data from inception to December 2014, to collect randomized controlled trials (RCTs) of LRYGB vs. LSG for obesity and T2DM. Two reviewers independently screened literature, extracted data and evaluated the risk of bias of included studies. Then, meta-analysis was performed using RevMan 5.2 software. Results A total of 8 RCTs including 828 patients were included. The results of meta-analysis showed that: There were no significant differences between the LRYGB group and the LSG group in body mass index (MD= –1.02, 95%CI –2.90 to 0.86, P=0.29), remission rate of T2DM (OR=1.11, 95%CI 0.71 to 1.73, P=0.64), reoperation rate (OR=2.74, 95%CI 1.01 to 7.42, P=0.05), level of fasting plasma glucose (MD=2.71, 95%CI -0.80 to 6.21, P=0.13), and level of serum low density lipoprotein (MD= –23.85, 95%CI –47.20 to –0.50, P=0.05). However, the LSG group had lower postoperative complication rate (OR=2.28, 95%CI 1.43 to 3.62, P=0.000 5) than that of the LRYGB group. Conclusion In short term, both LRYGB and LSG were equally efficient in the treatment of obesity and T2DM, but LSG has lower postoperative complication rate than LRYGB. Due to the limited quality and quantity of the included studies, more high quality studies are needed to verify the above conclusion. © 2015 Editorial Board of Chin J Evid-based Med.
Li H.,Henan University of Science and Technology |
Li H.,Puyang Oilfield General Hospital |
Zhou Y.,Shenzhen University |
Zheng Y.,Henan University of Science and Technology |
And 11 more authors.
Digestive Diseases and Sciences | Year: 2016
Background: Helicobacter pylori (H. pylori) is a well-recognized gastroduodenal pathogen and class I carcinogen. Dual oxidase-2 (DUOX2), a member of NADPH oxidase family, has several critical physiological functions, including thyroid hormone biosynthesis and host mucosal defense. Aim: To investigate the effect of H. pylori infection on DUOX2 gene expression in human stomach. Materials and Methods: The biopsies were obtained from patients who underwent endoscopic diagnosis. The patient serum was assayed for two virulence factors of H. pylori, CagA IgG and VacA. The inflammation in gastric mucosa was analyzed with histology. Real-time quantitative PCR was used to detect the expression of three members of NADPH oxidase, NOX1, NOX2, and DUOX2, as well as lactoperoxidase (LPO) in the gastric mucosa. NOX2, DUOX2, and myeloperoxidase (MPO) protein levels were quantified by Western blots or immunohistochemistry. Results: The H. pylori-infected gastric mucosa had more severe inflammation than uninfected samples. However, the expression of DUOX2 mRNA and protein was lower in gastric mucosa of patients with H. pylori infection compared to the uninfected. Among the H. pylori-infected patients, those having CagA IgG or VacA in the serum had lower DUOX2 expression levels than those infected with H. pylori without either virulence factor. The NOX2 and MPO levels were higher in those patients infected with H. pylori irrespective of the virulence factors than those uninfected patients. NOX1 and LPO mRNA were undetectable in the gastric mucosa. Conclusion: CagA+ or VacA+ H. pylori in the stomach of patients may suppress DUOX2 expression to promote its own survival. Increased NOX2 could not eliminate H. pylori infection. © 2016 The Author(s)
Zhou W.,Nanjing Medical University |
Zhao Y.,Nanjing Medical University |
Zhao Y.,Puyang Oilfield General Hospital |
Pan H.,Nanjing Medical University |
And 8 more authors.
Breast Cancer Research and Treatment | Year: 2016
We investigated whether great tumour burden in the primary draining lymph node would lead to obstructed lymphatic flow in breast cancer patients. Breast cancer patients with false-negative sentinel lymph nodes (SLNs) were enrolled from January 2001 to March 2011, retrospectively. A further 45 breast cancer patients were recruited prospectively from December 2013 to November 2014. Carbon nanoparticles, a lymphatic tracer, were injected into the subareolar area 24 h before surgery, followed by axillary lymph node dissection. In the SLN cohort, among the 28 false-negative cases, >50 % showed great tumour burden in the axilla. In the carbon nanoparticles cohort, we found that cases with <3 nodes involved in the pathology had more lymph nodes stained by carbon nanoparticles than the subgroup with ≥3 involved nodes (P = 0.003). Nodes stained with carbon nanoparticles showed smaller tumour burdens compared with unstained nodes (P < 0.05). Furthermore, five cases showed metastatic nodes that were not stained with carbon nanoparticles, and all the lymph nodes that were free of metastasis were stained with carbon nanoparticles. Great tumour burden in the axilla might lead to lymphatic flow obstructions in clinical practice. Nevertheless, clinical trials are still needed to validate our findings. © 2016, Springer Science+Business Media New York.
Zhang S.,Puyang Oilfield General Hospital |
Wang X.,Zhengzhou University |
Ju C.,Zhengzhou University |
Zhu L.,Zhengzhou University |
And 2 more authors.
International Immunopharmacology | Year: 2016
Objective To investigate the effects of Ca2 + activated potassium channel KCa3.1 and voltage-gated potassium channel Kv1.3 of B lymphocyte on inflammatory monocytes chemotaxis and the potential mechanisms. Materials and methods Thanswell test was used to detect the inflammatory monocyte (Ly-6Chi) chemotaxis caused by the B lymphocyte. Enzyme-linked immunosorbent assay (ELISA) was applied to detecting the C-C motif ligand 7 (CCL7) in cultured media. Cell counting kit-8 (CCK) was used to detect the proliferation of B lymphocytes after activation and blockage of both KCa3.1 and Kv1.3 channels. Western blot was used to detect the expression of phosphorylated extracellular signal-regulated kinase (P-ERK) of the B lymphocytes. Results When activated, B lymphocytes significantly proliferated. After application of KCa3.1 channel-specific inhibitor TRAM-34 and potent Kv1.3 channel inhibitor ShK, both B lymphocytes proliferation and Ly-6Chi monocyte chemotaxis were significantly inhibited. The expression of chemotaxis related factor CCL7 decreased remarkably. Conclusion The opening of KCa3.1 and Kv1.3 channels promote B lymphocyte activation, proliferation and Ly-6Chi monocyte chemotaxis. The increase of CCL7 secretion by B lymphocyte may explain the pro migration effects. © 2016 Elsevier B.V. All rights reserved.
Liu L.,Puyang Oilfield General Hospital |
Lai W.,Puyang Oilfield General Hospital
Journal of Clinical Dermatology | Year: 2016
Objective: To investigate the relationship between the invasiveness of Trichophyton rubrum and the infection site and the genotype through comparing the activity of keratinase produced by Trichophyton rubrum of different genotypes induced by different substrates in vitro. Methods: Two kinds of genotypes of Trichophyton rubrum strains, T1a and T1b, were cultured in vitro, using human fingernails, toenails, dandruff and hair as substrates respectively. The keratinase extraction was reacted with the keratin-azure and the keratinase activity was detected by the microplate reader. Results: Arranged in descending order, the keratinase activity of both T1a and T1b strains from Trichophyton rubrum respectively induced by different substrates was fingernails, toenails, dander and hair(P<0.05), while there was no significant difference between fingernails and toenails (P>0.05). There was no significant difference between the keratinase activity of T1a strain and T1b strain with the same substrate(P>0.05). Conclusions: The keratinase activity induced by different substrates is different. The invasiveness of Trichophyton rubrum are probably related to the substrate contained in the infection site, but not to the genotype.
Lu C.,Henan University of Traditional Chinese Medicine |
Jia H.,Henan University of Traditional Chinese Medicine |
Xu A.,Zhengzhou University |
Tang J.,Zhengzhou University |
And 3 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2014
Helicobacter pylori (Hp) infection is known to alter levels of pepsinogens (PG) and is correlated with several disease states, including gastric and cardiovascular diseases. This study sought to assess whether Hp infection is associated with hypertension as well as to identify the value of assessing the PG I/PG II ratio in patients with hypertension. The study included 396 individuals with hypertension who were assessed for infection with Hp by colloidal gold assay. Participants’ weight, height, blood pressure, and serum lipids were measured, and participants were examined for the presence of renal or ocular damage. H. pylori infection status or PG I/PG II ratio were compared against other variables (e.g., body mass index, serum cholesterol, diastolic blood pressure) by t-test or (Formula presented.) test, and Pearson’s correlation analysis was used to identify associations. Consistent with other studies, the PG I/PG II ratio of patients with Hp infection was significantly lower than that of patients without Hp infection (P < 0.001). The serum total cholesterol and triglycerides of patients with Hp infection were significantly higher than those of patients without Hp infection (P < 0.001), and the PG I/PG II ratio was negatively correlated with total cholesterol (r=-0.61) and triglycerides (r=-0.56) levels. However, there was no significant difference in hypertension severity by Hp infection status or PG I/PG II ratio. Interestingly, the PG I/PG II ratio was significantly lower in patients with hypertensive nephropathy or hypertensive retinopathy than in patients without these symptoms (P < 0.05). The areas under the receiver-operating characteristic curve were 0.77 and 0.83 in the diagnosis of nephropathy and retinopathy, respectively. These findings indicate that the PG I/PG II ratio is lower in individuals with hypertensive nephropathy and hypertensive retinopathy. Thus, the detection of the PG I/PG II ratio may be valuable for diagnostic screening for hypertensive organ damage. © 2014, E-Century Publishing Corporation. All rights reserved.
Liu C.,Puyang Oilfield General Hospital
International Eye Science | Year: 2015
AIM: To observe the clinical effect of on-flap and off-flap epipolis laser in situ keratomileusis (EPi-LASIK) in ametropia. METHODS: Sixty-eight myopia patients (136 eyes) receiving surgical treatment were selected and divided into research group and control group according to different therapies. The patients in research group adopted off-flap EPi-LASIK and those in control group adopted on-flap EPi-LASIK. The index like uncorrected visual acuity, diopter and Haze of two groups before surgery, 1wk, 1 and 4mo after surgery was observed. RESULTS: One month after surgery, the uncorrected visual acuity of research group was 1.33±0.22 while that of control group was 1.22±0.19 (P<0.05); Cylindrical diopter of research group was 0.10±0.55D while that of control group was 0.30±0.82D (P<0.05). One week after surgery, Haze of research group was 0.22±0.15 while that of control group was 0.23±0.18 (P<0.05). CONCLUSION: On-flap and off-flap EPi-LASIK are safe and effective surgery approaches in the clinical treatment of ametropia. The presence of corneal epithelial flap has a certain effect in the postoperative clinical outcome at early stage. The impact will be gradually reduced over time. Copyright 2015 by the IJO Press.
PubMed | Zhengzhou University, Puyang Oilfield General Hospital and Huazhong University of Science and Technology
Type: | Journal: International immunopharmacology | Year: 2016
To investigate the effects of Ca(2+) activated potassium channel KCa3.1 and voltage-gated potassium channel Kv1.3 of B lymphocyte on inflammatory monocytes chemotaxis and the potential mechanisms.Thanswell test was used to detect the inflammatory monocyte (Ly-6C(hi)) chemotaxis caused by the B lymphocyte. Enzyme-linked immunosorbent assay (ELISA) was applied to detecting the C-C motif ligand 7 (CCL7) in cultured media. Cell counting kit-8 (CCK) was used to detect the proliferation of B lymphocytes after activation and blockage of both KCa3.1 and Kv1.3 channels. Western blot was used to detect the expression of phosphorylated extracellular signal-regulated kinase (P-ERK) of the B lymphocytes.When activated, B lymphocytes significantly proliferated. After application of KCa3.1 channel-specific inhibitor TRAM-34 and potent Kv1.3 channel inhibitor ShK, both B lymphocytes proliferation and Ly-6C(hi) monocyte chemotaxis were significantly inhibited. The expression of chemotaxis related factor CCL7 decreased remarkably.The opening of KCa3.1 and Kv1.3 channels promote B lymphocyte activation, proliferation and Ly-6C(hi) monocyte chemotaxis. The increase of CCL7 secretion by B lymphocyte may explain the pro migration effects.