Puyang Academy of Forestry

Puyang, China

Puyang Academy of Forestry

Puyang, China
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Li Y.,Chinese Academy of Forestry | Fang W.,Chinese Academy of Forestry | Xie S.-J.,Puyang Academy of Forestry | Yang X.,Puyang Academy of Forestry | Wang L.-F.,Chinese Academy of Forestry
International Journal of Systematic and Evolutionary Microbiology | Year: 2017

A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strain, designated 2C-7T, was isolated from symptomatic bark of a Populus × euramericana canker. Growth occurred between 10 and 37°C and between pH 6 and 10, with optimal growth at 30°C and pH 7.0–8.0. Growth was present under 0–8% (w/v) salinity conditions (optimum 1–2 %). Growth occurred in the presence of 10mM chromium (Cr6+). The major fatty acids (≥10 %) of the novel strain were anteiso-C15:0, anteiso-C17:0 and iso-C16:0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and two unknown lipids. The major respiratory quinone was menaquinone MK-11. The cell wall amino acids were 2,4-diaminobutyric acid, alanine, glutamic acid and glycine. Strain 2C-7T was most similar to Leucobacter celer subsp. celer NAL101T (97.2% 16S rRNA gene sequence similarity), ‘Leucobacter kyeonggiensis’ F3-P9T (97.1 %) and Leucobacter chromiireducens L-1T (97.1 %). In the phylogenetic tree, the isolate formed a single distinct branch separate from those of L. celer subsp. celer NAL101T, ‘L. kyeonggiensis’ F3-P9T and Leucobacter chironomi DSM 19883T. The DNA–DNA hybridization values between the novel strain and the reference strains were lower than the accepted bacterial threshold level of 70% for species delineation. The DNA G+C content of strain 2C-7T was 70.0 mol%. Based on the data, strain 2C-7T represents a novel species in the genus Leucobacter, for which the name Leucobacter corticis sp. Nov. is proposed. The type strain is 2C-7T (=CFCC 11901T =KCTC 39643T). © 2017 IUMS.


Li Y.,Chinese Academy of Forestry | Guo L.-M.,Puyang Academy of Forestry | Chang J.-P.,Puyang Academy of Forestry | Xie S.-J.,Puyang Academy of Forestry | Piao C.-G.,Chinese Academy of Forestry
International Journal of Systematic and Evolutionary Microbiology | Year: 2016

Two Gram-staining-negative, aerobic, motile, slimy, glossy bacterial strains were isolated from bark tissue of Populus×euramericana. The bacteria grew at 10–37 °C, pH 5–10, with optimal growth at 28–30 °C, pH 6.0–8.0. Both strains grew with 0–3 % (w/v) NaCl. In the maximum-likelihood phylogenetic tree, the two isolates formed a distinct branch within the family Phyllobacteriaceae, and they were not closely related to any of the genera within the family Phyllobacteriaceae. The two novel isolates werepositive for oxidase andcatalase activity. The polar lipids profile revealed diphosphatidylglycerol, phosphatidylcholine, phospholipid, phosphatidylethanolamine, phosphatidylglycerol and five unknown lipids. The major fatty acids were C18: 1ω7c and C16: 0. The DNA G+C content was 56.4 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic data, the two strains represent a novel species belonging to a novel genus of the family Phyllobacteriaceae, for which the name Corticibacterium populi gen. nov., sp. nov. is proposed. The type strain of the type species is 16B10-2-7T (=CFCC 12884T=KCTC 42249T). © 2016 IUMS.


Li Y.,Chinese Academy of Forestry | He W.,Beijing Forestry University | Wang T.,Chinese Academy of Forestry | Wang T.,Sichuan Agricultural University | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

Two Gram-negative, non-motile, rod-shaped bacterial strains, 2BJ1T and 2C-3-1, were isolated from canker bark of Populus × euramericanacollected from different locations in Puyang City, Henan Province, China. The two strains were characterized using nutritional and physiological testing and DNA sequence analysis. They were found to produce acid from D-glucose. Haemolysis was not observed on agar medium supplemented with sheep erythrocytes. Phylogenetic analysis based on 16S rRNA, rpoB and gyrB gene sequences revealed that the strains formed a distinct cluster with 100 % bootstrap support within the genus Acinetobacter in all phylogenetic trees. The phenotypic characteristics most useful for the differentiation of the two strains from other species of the genus Acinetobacter were their ability to grow at 41 °C and to assimilate malonate, phenylacetate and trigonelline. Based on phenotypic, genotypic and phylogenetic characteristics, the two strains are considered to represent a novel species of the genusAcinetobacter, for which the name Acinetobacter qingfengensis sp. nov. is proposed. The type strain is 2BJ1T (= CFCC 10890T = KCTC 32225T). © 2014 IUMS.


Li Y.,Chinese Academy of Forestry | Li Y.,Beijing Forestry University | Piao C.-G.,Chinese Academy of Forestry | Ma Y.-C.,Beijing Forestry University | And 7 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

Two Gram-negative, non-motile, rod-shaped strains, BQ4-1T and NHI3-2, isolated respectively from the healthy and diseased part of Populus ×euramericana canker bark, were characterized using a polyphasic approach. Chemotaxonomic characterization supported the inclusion of the two strains in the genus Acinetobacter, with genomic DNA G+C contents (42.5-43 mol%) within the range observed for this genus (38-47 mol%) and 9-octadecenoic acid (C18: 1ω9c, 39.87 %), hexadecanoic acid (C16: 0, 11.26 %) and summed feature 3 (comprising C16: 1ω7c/C16: 1ω6c, 18.90 %) as major fatty acids. Phylogenetic analysis based on 16S rRNA, rpoB and gyrB gene sequences revealed that strains BQ4-1T and NHI3 did not cluster with any species with validly published names, and formed a distinct cluster with 99-100 % bootstrap support on three phylogenetic trees within the genus Acinetobacter. Acid was not produced from D-glucose, and haemolysis was not observed on agar media supplemented with sheep erythrocytes. On the basis of phenotypic, genotypic and phylogenetic characteristics, the two strains are considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter puyangensis sp. nov. is proposed. The type strain is BQ4-1T (= CFCC 10780T = JCM 18011T). © 2013 IUMS.


Li Y.,Beijing Forestry University | Li Y.,Chinese Academy of Forestry | Chang J.,Puyang Academy of Forestry | Guo L.-M.,Puyang Academy of Forestry | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

Five Gram-negative, non-motile, rod-shaped bacterial strains were isolated from cankers of Populus×euramericana collected from different locations in Puyang city, Henan Province, China. The five strains were characterized by nutritional and physiological testing and DNA sequence analysis. Haemolysis was not observed on agar media supplemented with sheep erythrocytes. The strains could be distinguished from members of most species of the genus Acinetobacter by their inability to assimilate L-arginine and benzoate. The five strains formed a single branch in phylogenetic trees based on 16S rRNA, gyrB and rpoB individual gene sequence analysis, indicating that they all belonged to a single taxon within the genus Acinetobacter. DNA–DNA hybridization results indicated that the five isolates represented to a single species that was separate from Acinetobacter puyangensis. On the basis of the phenotypic, genotypic and phylogenetic characteristics, the five strains are considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter populi sp. nov. is proposed. The type strain of A. populi sp. nov. is PBJ7T (CFCC 11170T=KCTC 42272T). © 2015 IUMS.


Li Y.,Chinese Academy of Forestry | Piao C.-G.,Chinese Academy of Forestry | Guo L.-M.,Puyang Academy of Forestry | Chang J.-P.,Puyang Academy of Forestry | And 4 more authors.
Forest Research | Year: 2013

In order to understand the predominant species dynamic and diversity of fungal endophytes in barks of Populus×euramericana cv. Robusta 94 and triploid of P. tomentosa,the fungal endophytes were isolated from barks of P.×euramericana cv. Robusta 94 and triploid of P. tomentosa by tissue isolation, and identified based on sequence analysis of the internal transcribed spacer and morphological characterization by microscopic observations. A total of 1 175 fungal endophytes were isolated from 996 bark tissues. The fungal endophytes were classified into 35 fungal taxa belonging to 15 genera, including 1 species of Basidiomycota and 34 taxa of Ascomycota. And Alternaria alternata, Botryosphaeria dothidea, Fusarium spp., and Diaporthe conorum were the predominant species of fungal endophytes in barks of P.×euramericana cv. Robusta 94 and triploid of P. tomentosa, while A. alternata and B. dothidea were the most common predominant species in the barks of the two cultivars. The variation of predominant fungal endophytes of two poplar species in different seasons was detected.

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