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Li Y.,Chinese Academy of Forestry | He W.,Beijing Forestry University | Wang T.,Chinese Academy of Forestry | Wang T.,Sichuan Agricultural University | And 5 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2014

Two Gram-negative, non-motile, rod-shaped bacterial strains, 2BJ1T and 2C-3-1, were isolated from canker bark of Populus × euramericanacollected from different locations in Puyang City, Henan Province, China. The two strains were characterized using nutritional and physiological testing and DNA sequence analysis. They were found to produce acid from D-glucose. Haemolysis was not observed on agar medium supplemented with sheep erythrocytes. Phylogenetic analysis based on 16S rRNA, rpoB and gyrB gene sequences revealed that the strains formed a distinct cluster with 100 % bootstrap support within the genus Acinetobacter in all phylogenetic trees. The phenotypic characteristics most useful for the differentiation of the two strains from other species of the genus Acinetobacter were their ability to grow at 41 °C and to assimilate malonate, phenylacetate and trigonelline. Based on phenotypic, genotypic and phylogenetic characteristics, the two strains are considered to represent a novel species of the genusAcinetobacter, for which the name Acinetobacter qingfengensis sp. nov. is proposed. The type strain is 2BJ1T (= CFCC 10890T = KCTC 32225T). © 2014 IUMS. Source


Li Y.,Chinese Academy of Forestry | Piao C.-G.,Chinese Academy of Forestry | Guo L.-M.,Puyang Academy of Forestry | Chang J.-P.,Puyang Academy of Forestry | And 4 more authors.
Forest Research | Year: 2013

In order to understand the predominant species dynamic and diversity of fungal endophytes in barks of Populus×euramericana cv. Robusta 94 and triploid of P. tomentosa,the fungal endophytes were isolated from barks of P.×euramericana cv. Robusta 94 and triploid of P. tomentosa by tissue isolation, and identified based on sequence analysis of the internal transcribed spacer and morphological characterization by microscopic observations. A total of 1 175 fungal endophytes were isolated from 996 bark tissues. The fungal endophytes were classified into 35 fungal taxa belonging to 15 genera, including 1 species of Basidiomycota and 34 taxa of Ascomycota. And Alternaria alternata, Botryosphaeria dothidea, Fusarium spp., and Diaporthe conorum were the predominant species of fungal endophytes in barks of P.×euramericana cv. Robusta 94 and triploid of P. tomentosa, while A. alternata and B. dothidea were the most common predominant species in the barks of the two cultivars. The variation of predominant fungal endophytes of two poplar species in different seasons was detected. Source


Li Y.,Chinese Academy of Forestry | Li Y.,Beijing Forestry University | Piao C.-G.,Chinese Academy of Forestry | Ma Y.-C.,Beijing Forestry University | And 7 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

Two Gram-negative, non-motile, rod-shaped strains, BQ4-1T and NHI3-2, isolated respectively from the healthy and diseased part of Populus ×euramericana canker bark, were characterized using a polyphasic approach. Chemotaxonomic characterization supported the inclusion of the two strains in the genus Acinetobacter, with genomic DNA G+C contents (42.5-43 mol%) within the range observed for this genus (38-47 mol%) and 9-octadecenoic acid (C18: 1ω9c, 39.87 %), hexadecanoic acid (C16: 0, 11.26 %) and summed feature 3 (comprising C16: 1ω7c/C16: 1ω6c, 18.90 %) as major fatty acids. Phylogenetic analysis based on 16S rRNA, rpoB and gyrB gene sequences revealed that strains BQ4-1T and NHI3 did not cluster with any species with validly published names, and formed a distinct cluster with 99-100 % bootstrap support on three phylogenetic trees within the genus Acinetobacter. Acid was not produced from D-glucose, and haemolysis was not observed on agar media supplemented with sheep erythrocytes. On the basis of phenotypic, genotypic and phylogenetic characteristics, the two strains are considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter puyangensis sp. nov. is proposed. The type strain is BQ4-1T (= CFCC 10780T = JCM 18011T). © 2013 IUMS. Source


Li Y.,Beijing Forestry University | Li Y.,Chinese Academy of Forestry | Chang J.,Puyang Academy of Forestry | Guo L.-M.,Puyang Academy of Forestry | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

Five Gram-negative, non-motile, rod-shaped bacterial strains were isolated from cankers of Populus×euramericana collected from different locations in Puyang city, Henan Province, China. The five strains were characterized by nutritional and physiological testing and DNA sequence analysis. Haemolysis was not observed on agar media supplemented with sheep erythrocytes. The strains could be distinguished from members of most species of the genus Acinetobacter by their inability to assimilate L-arginine and benzoate. The five strains formed a single branch in phylogenetic trees based on 16S rRNA, gyrB and rpoB individual gene sequence analysis, indicating that they all belonged to a single taxon within the genus Acinetobacter. DNA–DNA hybridization results indicated that the five isolates represented to a single species that was separate from Acinetobacter puyangensis. On the basis of the phenotypic, genotypic and phylogenetic characteristics, the five strains are considered to represent a novel species of the genus Acinetobacter, for which the name Acinetobacter populi sp. nov. is proposed. The type strain of A. populi sp. nov. is PBJ7T (CFCC 11170T=KCTC 42272T). © 2015 IUMS. Source

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