Putuo District Center Hospital

Shanghai, China

Putuo District Center Hospital

Shanghai, China
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Zhao X.,Fudan University | Zhao X.,Ningbo University | Xu Y.,West Virginia University | Zhao Q.,Putuo District Center Hospital | And 3 more authors.
Neuropharmacology | Year: 2012

Curcumin, a phenolic compound present in Curcuma longa, has been reported to exert antinociceptive effects in some animal models, but the mechanisms remain to be elucidated. This work aimed to investigate the antinociceptive action of curcumin on neuropathic pain and the underlying mechanism(s). Chronic constriction injury (CCI), a canonical animal model of neuropathic pain, was produced by loosely ligating the sciatic nerve in mice and von Frey hair or hot plate test was used to assess mechanical allodynia or thermal hyperalgesia (to heat), respectively. Chronic, but not acute, curcumin treatment (5, 15 or 45 mg/kg, p.o., twice per day for three weeks) alleviated mechanical allodynia and thermal hyperalgesia in CCI mice, accompanied by increasing spinal monoamine (or metabolite) contents. Chemical ablation of descending noradrenaline (NA) by 6-hydroxydopamine (6-OHDA), or depletion of descending serotonin by p-chlorophenylalanine (PCPA), abolished curcumin's antinociceptive effect on mechanical allodynia or thermal hyperalgesia, respectively. The anti-allodynic action of curcumin on mechanical stimuli was totally blocked by chronic co-treatment with the β 2-adrenoceptor antagonist ICI 118,551, or by acute co-treatment with the delta-opioid receptor antagonist naltrindole. Meanwhile, co-treatment with the 5-HT 1A receptor antagonist WAY-100635 chronically, or with the irreversible mu-opioid receptor antangonist β-funaltrexamine acutely, completely abrogated the anti-hyperalgesic action of curcumin on thermal stimuli. Collectively, these findings indicate that the descending monoamine system (coupled with spinal β 2-adrenoceptor and 5-HT 1A receptor) is critical for the modality-specific antinociceptive effect of curcumin in neuropathic pain. Delta- and mu-opioid receptors are likely rendered as downstream targets, accordingly. © 2011 Elsevier Ltd. All rights reserved.


Wu Y.-C.,Shanghai JiaoTong University | Zhang J.-F.,Shanghai JiaoTong University | Sun Y.-J.,Shanghai JiaoTong University | Huang C.-F.,Shanghai JiaoTong University | And 2 more authors.
Journal of Acupuncture and Tuina Science | Year: 2013

Objective: To compare the therapeutic efficacies between electroacupuncture (EA) and medication for cervical intervertebral disc herniation (CIDH). Methods: Totally 420 patients with CIDH were randomized into two groups by random number table. 210 patients in the EA group were intervened by EA at Dazhui (GV 14), Dazhu (BL 11), and Houxi (SI 3); 210 patients in the medication group were treated by oral administration of Meloxicam tablets. Results: The average ranks of both short and long term efficacies in the EA group were significantly lower than that in the medication group (both P<0.01); there were significant differences in comparing the total effective rates of both short and long terms between the two groups (both P<0.01). It shows that EA group has better therapeutic efficacy than the medication group. Conclusion: EA is better than medication in comparing both short-term and long-term therapeutic efficacies in treating CIDH. © 2013 Shanghai Research Institute of Acupuncture and Meridian and Springer-Verlag Berlin Heidelberg.


Lou L.,Beijing University of Chinese Medicine | Liu Y.,Putuo District Center Hospital | Zhou J.,Beijing University of Chinese Medicine | Wei Y.,Beijing University of Chinese Medicine | And 3 more authors.
Immunopharmacology and Immunotoxicology | Year: 2015

Context: Chlorogenic acid (CGA) and luteolin (Lut) are the predominant constituents of Caulis Lonicerae, which is usually used in the treatment for rheumatoid arthritis (RA).Objective: In this study, we investigated whether CGA and Lut could synergistically inhibit the proliferation of fibroblast-like synoviocytes (FLSs) in RA synovial tissues.Methods: Rat FLS cells (RSC-364) induced by interleukin (IL)-1β were treated by CGA, Lut or both of them. The apoptosis rates were detected by flow cytometer. Protein expression of key molecules of NF-B and JAK/STAT signaling pathways were detected by Western blot.Results and discussion: Treatment with CGA and Lut inhibited the proliferation of RSC-364 cells stimulated by IL-1β significantly and induced cell apoptosis notably. The ratio of apoptosis in RSC-364 cells induced with IL-1β accompanied by both CGA and Lut increased approximately 7-fold compared with those incubated with IL-1β alone. The results of immunoblot analysis revealed that the key molecules involved in the NF-B and JAK/STAT-signaling pathways, including NF-B p50, p100, IKKα/β, gp103, JAK1 and STAT3, were decreased significantly in RSC-364 cells treated by IL-1β plus CAG and Lut compared with those incubated with IL-1β alone. Additionally, the amounts of phospho-IKKα/β and phospho-STAT3 were also decreased significantly in cells treated with CGA and Lut. Furthermore, the synergistic effect of CGA and Lut was superior to the effect of one of these two ingredients.Conclusion: Our finding suggested that the combination of CGA and Lut may be a potential therapeutic treatment for the inflammatory proliferation of synoviocytes in patients with RA. © 2015 Taylor & Francis.


PubMed | Beijing University of Chinese Medicine, Guangxi University and Putuo District Center Hospital
Type: Journal Article | Journal: Immunopharmacology and immunotoxicology | Year: 2015

Chlorogenic acid (CGA) and luteolin (Lut) are the predominant constituents of Caulis Lonicerae, which is usually used in the treatment for rheumatoid arthritis (RA).In this study, we investigated whether CGA and Lut could synergistically inhibit the proliferation of fibroblast-like synoviocytes (FLSs) in RA synovial tissues.Rat FLS cells (RSC-364) induced by interleukin (IL)-1 were treated by CGA, Lut or both of them. The apoptosis rates were detected by flow cytometer. Protein expression of key molecules of NF-B and JAK/STAT signaling pathways were detected by Western blot.Treatment with CGA and Lut inhibited the proliferation of RSC-364 cells stimulated by IL-1 significantly and induced cell apoptosis notably. The ratio of apoptosis in RSC-364 cells induced with IL-1 accompanied by both CGA and Lut increased approximately 7-fold compared with those incubated with IL-1 alone. The results of immunoblot analysis revealed that the key molecules involved in the NF-B and JAK/STAT-signaling pathways, including NF-B p50, p100, IKK/, gp103, JAK1 and STAT3, were decreased significantly in RSC-364 cells treated by IL-1 plus CAG and Lut compared with those incubated with IL-1 alone. Additionally, the amounts of phospho-IKK/ and phospho-STAT3 were also decreased significantly in cells treated with CGA and Lut. Furthermore, the synergistic effect of CGA and Lut was superior to the effect of one of these two ingredients.Our finding suggested that the combination of CGA and Lut may be a potential therapeutic treatment for the inflammatory proliferation of synoviocytes in patients with RA.


PubMed | Beijing University of Chinese Medicine, Guangxi University and Putuo District Center Hospital
Type: Journal Article | Journal: Experimental and therapeutic medicine | Year: 2016

Chlorogenic acid (CGA) is the primary constituent of Caulis


Huang D.,Fudan University | Qian J.,Fudan University | Ge L.,Fudan University | Jin X.,Fudan University | And 8 more authors.
American Heart Journal | Year: 2012

Background: No randomized trial has been conducted to compare different vasodilators for treating no-reflow during primary percutaneous coronary intervention (PCI) for ST-segment elevation acute myocardial infarction. Methods: The prospective, randomized, 2-center trial was designed to compare the effect of 3 different vasodilators on coronary no-reflow. A total of 102 patients with no-reflow in primary PCI were randomized to receive intracoronary infusion of diltiazem, verapamil, or nitroglycerin (n = 34 in each group) through selective microcatheter. The primary end point was coronary flow improvement in corrected thrombolysis in myocardial infarction frame count (CTFC) after administration of the drug. Results: Compared with that of the nitroglycerin group, there was a significant improvement of CTFC after drug infusion in the diltiazem and verapamil groups (42.4 frames vs 28.1 and 28.4 frames, P <.001). The improvement in CTFC was similar between the diltiazem and verapamil groups (P =.9). Compared with the nitroglycerin group, the diltiazem and verapamil groups had more complete ST-segment resolution at 3 hours after PCI, lower peak troponin T level, and lower N-terminal pro-B-type natriuretic peptide levels at 1 and 30 days after PCI. After drug infusion, the drop of heart rate and systolic blood pressure in the verapamil group was greater than that in the diltiazem and nitroglycerin groups. Conclusion: Intracoronary infusion of diltiazem or verapamil can reverse no-reflow more effectively than nitroglycerin during primary PCI for acute myocardial infarction. The efficacy of diltiazem and verapamil is similar, and diltiazem seems safer. © 2012 Mosby, Inc. All rights reserved.


Wang H.-M.,Putuo District Center Hospital | Zhang X.-R.,Putuo District Center Hospital
International Journal of Ophthalmology | Year: 2010

Small heat shock proteins (sHSPs), sHSPs are the subunit of heat shock protein (HSP), which will express increased rapidly with a variety of stress state and take part in the protection and injury of organization. The research of sHSPs has attracted extensive attention from scholars at home and abroad. sHSPs play an important role in chaperone, collaborative immunization, inhibiting apoptosis, antigen-presenting and steroid hormone receptor. To fully recognize the mechanism of sHSPs in eye diseases can help understand the pathogenesis of eye diseases further and provide a new basis of molecular biology for the treatment of eye diseases.


Zhang H.-Y.,Shanghai University of Traditional Chinese Medicine | Cao X.-D.,Shanghai University of Traditional Chinese Medicine | Chen J.-J.,Shanghai University of Traditional Chinese Medicine | Luo Y.-Q.,Shanghai University of Traditional Chinese Medicine | Wang X.-C.,Putuo District Center Hospital
European Review for Medical and Pharmacological Sciences | Year: 2015

A patient had right upper quadrant pain with sclera was transferred from emergency room to the hospital, she was proposed to have acute cholecystitis, gallstones, obstructive jaundice, and a four-year history of gallbladder stones. The NMR results showed that the gallbladder was significantly enlarged and the gallbladder wall was thickening irregularly. The liver morphology was not abnormal except with extensive intrahepatic bile duct dilatation. The MRCP results demonstrated that the intrahepatic bile ducts were significant expanded. The ERCP results showed that duodenal stenosis and extra-hepatic bile duct stenosis. We placed a plastic stent of 8.5Fr and 12 cm in length in the hepatic duct, and after biliary plastic stent placement, jaundice was rapidly reduced and liver function was improved significantly. A surgery was performed and the final pathologic diagnosis is a complication of Xanthogranulomatous cholecystitis with Mirizzi syndrome. After the surgery of cholecystectomy and a bile duct repair were performed, the patient was recovered well. Conclusively, if a patient was diagnosed as biliary stricture, a biliary metal stent should not be placed until pathological diagnosis of malignancy.


Tao R.,Putuo District Center Hospital | Tu S.,Putuo District Center Hospital | Liu C.,Putuo District Center Hospital | Yang Q.,Putuo District Center Hospital | And 2 more authors.
OncoTargets and Therapy | Year: 2015

Background: With the development of proteomics, tumor markers have attracted increasing attention for the early diagnosis and treatment of lung cancer. As biochip technology and nanotechnology continues to grow, rapid and highly sensitive joint detection of multi-tumor markers has become possible. Methods: Eighty-six patients with lung cancer and 42 healthy controls were recruited for this study. Based on analysis of the detection results, we plotted four standard tumor marker graphs, and compared the results of the highly sensitive nanogold probe and protein chip detection with the results of electrochemiluminescence immunoassay and Dickkopf-1 (DKK1) detection used in the clinic. We then analyzed the relationship between the detection results and our clinical data. Results: Four plotted standard protein graphs all had stages with sound linear relationships. It was found in a correlation analysis of the detection results that overall the two methods showed consistency. Conclusion: We developed a detection method for ultra-trace protein that can detect four tumor markers, namely carcinoembryonic antigen, cytokeratin-19 fragments, neuron-specific enolase, and DKK1 in a highly sensitive way within 1.5 hours by magnifying the signal of nanogold deposition based on protein chips and nanogold probes. By comparing the results from the different detection devices, we have developed an experimental basis for detection of tumor markers in the clinic. © 2015 Tao et al.


PubMed | Putuo District Center Hospital
Type: | Journal: OncoTargets and therapy | Year: 2015

With the development of proteomics, tumor markers have attracted increasing attention for the early diagnosis and treatment of lung cancer. As biochip technology and nanotechnology continues to grow, rapid and highly sensitive joint detection of multi-tumor markers has become possible.Eighty-six patients with lung cancer and 42 healthy controls were recruited for this study. Based on analysis of the detection results, we plotted four standard tumor marker graphs, and compared the results of the highly sensitive nanogold probe and protein chip detection with the results of electrochemiluminescence immunoassay and Dickkopf-1 (DKK1) detection used in the clinic. We then analyzed the relationship between the detection results and our clinical data.Four plotted standard protein graphs all had stages with sound linear relationships. It was found in a correlation analysis of the detection results that overall the two methods showed consistency.We developed a detection method for ultra-trace protein that can detect four tumor markers, namely carcinoembryonic antigen, cytokeratin-19 fragments, neuron-specific enolase, and DKK1 in a highly sensitive way within 1.5 hours by magnifying the signal of nanogold deposition based on protein chips and nanogold probes. By comparing the results from the different detection devices, we have developed an experimental basis for detection of tumor markers in the clinic.

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